한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

Elevated TRAF2/6 expression in Parkinson's disease is caused by the loss of Parkin E3 ligase activity.

Chung, Ji-Yun,Park, Hee Ra,Lee, Su-Jin,Lee, Sun-Hye,Kim, Jin Sik,Jung, Youn-Sang,Hwang, Sang Hyun,Ha, Nam-Chul,Seol, Won-Gi,Lee, Jaewon,Park, Bum-Joon United States and Canadian Academy of Pathology [e 2013 Laboratory investigation Vol.93 No.6

<P>Parkinson's disease (PD) is the second leading neurodegenerative disease, and is known to be induced by environmental factors or genetic mutations. Among the verified genetic mutations of PD, Parkin, isolated from the PARK2 locus, shows an autosomal recessive inheritance pattern and is known to be an E3 ligase. However, the physiological target of Parkin and the molecular mechanism of Parkin-deficiency-induced PD have not been clearly demonstrated until now. It has recently been proposed that inflammation, suggesting as a causal factor for PD, is enhanced by Parkin deficiency. Thus, we examined the relationship between inflammation-related factors and Parkin. Here, we provide the evidence that Parkin suppresses inflammation and cytokine-induced cell death by promoting the proteasomal degradation of TRAF2/6 (TNF-α receptor-associated factor 2/6). Overexpression of Parkin can reduce the half-lives of TRAF2 and TRAF6, whereas si-Parkin can extend them. However, mutant Parkins did not alter the expression of TRAF2/6. Thus, loss of Parkin enhances sensitivity to TNF-α- or IL-1β-induced JNK activation and NF-κB activation. Indeed, si-Parkin-induced apoptosis is suppressed by the knockdown of TRAF6 or TRAF2. We also observed elevated expression levels of TRAF6 and a reduction of IκB in an 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced PD mouse model. Moreover, elevated expression levels or aggregation of TRAF6 were detected in approximately half of the human PD tissues (7/15 cases) and 2 cases, respectively. In addition, TRAF6 and Parkin expression levels show a reverse relationship in human PD tissues. Our results strongly suggest that the reduction of Parkin or overexpression of TRAF2/6 by chronic inflammation would be the reason for occurrence of PD.</P>

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

식이내 율무함량이 Rat 의 생식작용에 미치는 영향

박윤진,신덕규 청주대학교 교육문제연구소 1998 교육과학연구 Vol.11 No.3

This study were carried out to investigate the effect of Job's tears amount in diet on the reproduction function of Rats. The rats were divided in 8 groups which were fed basal diet(A), 40% Job's tears diet(B), 50% Job's tears group(C), 60% Job's tears group(D) Job's tears were revealed on the effect of reproduction of Rats. 60% Job's tears Diet group were showed 5% pregnancy, 50% Job's tears diet group were showed 66% pregnancy, 40% Job's tears diet group were showed 85.7% pregnancy higher than control group.

운동훈련에 따른 활성산소의 생성과 조직손상

박윤진 청주대학교 학술연구소 2008 淸大學術論集 Vol.12 No.-

This research was investigated study for production and system injure of ROS by exercise and training. More carbohydrate was consumed as exercise energy in strong intensity exercise. The continuous exercise time is long in low intensity exercise as sufficient oxygen supply so that the increase of fat acids depend on energy supply of fat. Main factors of decision are acid of cells and feedback balance state in continuously co-function. Activity physical exercise is increasing fat triglyceride cause to oxygen free-radical production occur to acid stress of body. DNA injure by regular training is insignificancy difference than pre-training in measurement results during 24 hours.

심장질환 재활을 위한 운동요법

朴潤珍 청주대학교 학술연구소 2007 淸大學術論集 Vol.9 No.-

The purpose of this study was to investigate the exercise prescription for CVD disease rehabillitation. 1st, heredic factor, living habit of 20-30generation were to prognostic posibly CVD disease and risk fact after middle-age period. CVD disease and prognostic children period and adult obesity was come out Co-disease that to prevent middle-age period CVD disease as hopable living habit form in young people period. 2nd, extreme high dentisity exercise was occure muscle hurt that to effect negatively which increasing circulation of vitalmeterial an inflammation immunity reaction. 3rd, high relating fat prognostic about CVD disease that relate to VDL-C/HDL-C, TD/HDL-C rate or increasing TG AND TC/HDL-C rate which were effected through whole generation. 1st, exercise items for CVD disease rehabillitation patients were effective to prevent circulation disease which was walking, bicycling or jogging and cross country-running. 2nd, aerobic endurance exercise was care for breathing method in cross country-ski, swimming as aerobic exercise that to inject an irregular pulse under 27℃ water, to escape climing big curve that heavy materials instrument deliver as golf. 3rd, pingpong was hopable that enjoying recreation standard which tennis and bedminton are need to escape for participating game. 4th, This exercise are not moderate that climbing, a downhill race-ski, soccur, volleyball, handball, short running-track or throwing and high-jump. Exercise prescription for CVD disease rehabillitation patients was to practice which 1st, 1st period among enter-hospital period for come back society practically as purpose of exercise capacity, increase 2nd period as out-hospital to prevent reoccure and maintain health after back to society to practice divided 3rd period that indicate exercise prescription period. 2nd, to practice exercise prescription were not occure as serious co-disease among 1st period and 2nd period about patients exercise-cure, 1st periods exercise prescription was pationts age, disease measure, co-diseases therefore to practice most of them was difference in exercise that 3-6 weeks as 10 steps was practiced. 3rd, to practice ready ECG, emergency demand set up place of 1st period patients and 2nd period patients that are effective 8 weeks or 10 weeks and 12 weeks in exercise prescription. 4th, 3th period people are to moderate selfish each situation in home, health club to exercise regularly that constantly or continually and slowly is important which exercise intensity was to recommend 60-90% of max-HR, 50-80% of max-Oxygen intake amount.

음식물 쓰레기 퇴비가 오이 발아와 유묘생장에 미치는 영향

박진영,조경철,김희경,지연태,정순주 全南大學校 農業科學技術硏究所 2001 農業科學技術硏究 Vol.36 No.-

This study was conducted to find out toxicity of compost made of food waste to cucumber seed germination and seedlings growth when it was mixed with Mixlite for bed substrate. Mixlite was solely used as bed soil, or mixed with food waste compost ranging 1% to 100% (w/w). Germination percentage and seedling growth of cucumber were examined in 4 weeks after seedling. When cucumber seeds were sowed in Mixlite mixed with food waste compost at 100, 50, 30, 20, 10 or 1%, germination percentages were 30, 36, 38, 46, 74 and 84% compared to with control(100%), respectively. It showed that germination percentage percentage was adversely affected by the amount of food waste composed mixed in the bed substract. Plant height and hypocotyls diameter, root length and root fresh weight, hypocotyls fresh weight and cotyledon fresh weight of cucumber seedling were similarly adversely affected by the amount of food waste compost. Optimum content of the food waste compost for cucumber nursery substrate was found to be 1% of Mixlite.

운동부하에 따른 지질대사산물과 노화작용

박윤진 청주대학교 교육문제연구소 1999 교육과학연구 Vol.13 No.-

영양의 불균형과 노화에 관한 연구

박윤진 淸州大學校 學術硏究所 2006 淸大學術論集 Vol.7 No.-

The purpose of this study is to get the problem of nutritional unbalance and aging in modem living not only obesity by over-intake but also low-weight disease by nutritional control and social function in which cause to starvation condition and environment cause of inherent, posteriori disease which to prevent this disease and aging to gain material of life-long healthy that the results from this study are as follows. To continue adequate intake condition and to contain fuel(especially fat) starvation condition not consume all of them which nutritional unbalance contain fat into obesity. After 2000 years revealed marvelously low-weight, low-birth, low-bone density, low-rice consume statement by starvation excellently to risk healthy of women and children. We need to applicate for prevent of disease and aging that to resolve the nutritional unbalance and starvation according to universal food life change consist of (Estimated Average Requirement, EAR), (Recommended Intake, RI), (Adequate Intake, AI), (Tolerable Upper Intake Level, UL), to decide (Dietary Reference Intakes; DRIs) in korean nutrition recommended intake. Aging was expected one of them expect-change which change of obesity-feed cycle and exercise cycle that this time shows to decrease sense density about hormone of main organization in body, as a result of starvation-feed cycle transfer organization normal reaction decrease ability. Aging is decide to factor limited age of people that relate to aging of man and the old disease. Aging and exercise metabolism statement is in decrease nutrition, hormone shows in metabolic organization as metabolic corelation among organization.