Identification of a sensitive urinary biomarker, selenium-binding protein 1, for early detection of acute kidney injury

Kim, Kyeong Seok,Yang, Hun Yong,Song, Hosup,Kang, Ye Rim,Kwon, JiHoon,An, JiHye,Son, Ji Yeon,Kwack, Seung Jun,Kim, Young-Mi,Bae, Ok-Nam,Ahn, Mee-Young,Lee, Jaewon,Yoon, Sungpil,Lee, Byung μ,Kim, Hyung TAYLOR & FRANCIS 2017 Journal of Toxicology and Environmental Health Vol.80 No.9

<P>Acute kidney injury (AKI) is associated with increased mortality rate in patients but clinically available biomarkers for disease detection are currently not available. Recently, a new biomarker, selenium-binding protein 1 (SBP1), was identified for detection of nephrotoxicity using proteomic analysis. The aim of this study was to assess the sensitivity of urinary SBP1 levels as an early detection of AKI using animal models such as cisplatin or ischemia/reperfusion (I/R). Sprague-Dawley rats were injected with cisplatin (6 mg/kg, once i.p.) and sacrificed at 1, 3, or 5 days after treatment. Ischemia was achieved by bilaterally occluding both kidneys with a microvascular clamp for 45 min and verified visually by a change in tissue color. After post-reperfusion, urine samples were collected at 9, 24, and 48 hr intervals. Urinary excretion of protein-based biomarkers was measured by Western blot analysis. In cisplatin-treated rats, mild histopathologic alterations were noted at day 1 which became severe at day 3. Blood urea nitrogen (BUN) and serum creatinine (SCr) levels were significantly increased at day 3. Levels of urinary excretion of SBP1, neutrophil gelatinase-associated lipocalin (NGAL), and a tissue inhibitor of metalloproteinase-1 (TIMP-1) were markedly elevated at day 3 and 5 following drug treatment. In the vehicle-treated I/R group, serum levels of BUN and SCr and AST activity were significantly increased compared to sham. Urinary excretion of SBP1 and NGAL rose markedly following I/R. The urinary levels of SBP1, NGAL, TIMP-1, and KIM-1 proteins excreted by AKI patients and normal subjects were compared. Among these proteins, a marked rise in SBP1 was observed in urine of patients with AKI compared to normal subjects. Based upon receiver-operator curves (ROC), SBP1 displayed a higher area under the curve (AUC) scores than levels of SCr, BUN, total protein, and glucose. In particular, SBP1 protein was readily detected in small amounts of urine without purification. Data thus indicate that urinary excretion of SBP1 may be useful as a reliable biomarker for early diagnosis of AKI in patients.</P>

비장애 아동과 전반적 발달장애 아동에서 회전 후 안구진탕 기간의 비교

김진미,송지원,홍은경,김성희,김경미 대한감각통합치료학회 2005 대한감각통합치료학회지 Vol.3 No.1

Objective : The purpose of this study was to get the mean of duration of post-rotatory nystagmus test in normal children and to differentiate the duration of post-rotatory nystagmus test between normal children and children with pervasive developmental disorder. Method : 84 subjects were between 3 and 5 years of age and consisted of 64 normal children and 20 children with the pervasive developmental disorder. Analysis of the data was done by using t-test and ANOVA. Results : The results were as follows: 1. Range of duration of post-rotatory nyatagmus test in normal children was 5-22second on left and 7-21 second on right and the mean was 12.63 second on left and 12.59 second on right. 2. Range of duration of post-rotatory nystagmus test in children with the pervasive developmental disorder was 3-11 second on both and the mean was 5.65 second on left and 5.60 second on right. 3. There was no significant difference between males and females with normal children in duration of post-rotatory nystagmus test. However, there was a significant difference of the mean duration between 3 and 5 years old normal group. 4. Children with pervasive developmental disorder significantly have relatively lower duration than the duration of post-rotatory nystagmus test of normal children. Conclusions : The results of the study showed significant difference between normal children and children with pervasive developmental disorder in duration of post-rotatory nystagmus test and suggest that they could be applied to the baseline of clinical therapy.

위절제술 환자의 표준진료지침 개발 및 적용 효과

김은희,김철규,이순교,김순덕,이혜옥,권정순,이경미,이민미,심순미,유용만,신종식,강은희,이상일,김병식,오성태,육정환,박수길 한국의료QA학회 2003 한국의료질향상학회지 Vol.10 No.2

Background : Gastric cancer is the most common malignant tumor in Korea. surgical operation is one of the major treatment modalities for gastric cancer patients. Therefore, gastrectomy is one of the most common procedures in General Surgery. There were variation in length of hospital stay and medical treatment for gastrectomy between three surgeons at Asan Medical Center. Clinical pathways have received considerable attention as a tool for recucing the medical practice variation, increasing the efficiency of care process, and improving the quality of care. The aim of this study was to evaluate the effect of a clinical pathway for gastrectomy in gastric cancer patients. Methods : The clinical pathway for gastrectomy was developed and implemented by a multidisciplinary group in Asan Medical Center. A computerized clinical pathway program was developed and revised after a pilot test. A total of 145 patients underwent gastrectomy by three surgeons at Asan Medical Center. We compared the length of hospital stay, patient satisfaction, and unplanned readmission rate between the pre-pathway group(n=67) and the post-pathway group(n=78). We also investigated the degree of satisfaction among the physicians and nurses who were main end-users of the clinical pathway. Results : The clinical pathway was applied to all target patients. The average length of hospital stay was shortened from 12.7days to 10.6days(p<0.01). The degree of patient satisfaction with the care process changed from 90.3% to 89.2% after the implementation of the clinical pathway, but the difference was of satistically significant(p=0.761). Unplanned readmission rate was 2.9% in the pre-pathway group. More than 90% of physicians and nurses answered that the clinical pathway had been a useful tool in their medical practice. Conclusions : The findings of the study demonstrated that implementation of the clinical pathway for gastrectomy produced substantial reduction in the length of hospital stay while improving the quality of patient outcomes. The computerized clinical pathway program can be used as one of the powerful patient management tools for reducing the practice variations and increasing the efficiency of care process in Korea hospital settings.

관상동맥 스텐트 삽입술을 시행한 환자에서 안지오텐신 전환효소 억제제와 안지오텐신 수용체 차단제가 재협착에 미치는 영향

김연경,정진원,윤경호,박은미,송미진,최준호,이은미,유남진,김남호,오석규 圓光大學校 醫科學硏究所 2006 圓光醫科學 Vol.21 No.1

민들레 잎과 뿌리 분말을 첨가한 설기떡의 품질 특성

유경미,김세희,장정화,황인경,김경임,김성수,김영찬 한국조리과학회 2005 한국식품조리과학회지 Vol.21 No.1

In order to develop a dandelion (Taraxacum officinale) with natural food applications, the effects of dandelion leaves and roots powder content on the physical, textural and sensory properties of sulggidduk were examined. Dandelion powders of leaves and roots at 0, 1, 2 and 3% were added to the dandelion sulgidduk. In the dandelion roots powder in sulgidduk, the color intensity of L values and the values and moisture content decreased however, the sensory color intensity, dandelion flavor and overall acceptability increased with increasing the dandelion powder content. The sensory overall acceptability indicated the dandelion roots powder content at 3% had the highest overall and flavor scores. As the dandelion leaves content was increased, the moisture contents, lightness, adhesiveness and cohesiveness of sulggidduk decreased, while the sensory color intensity, sensory hardness, hardness of texture properties and dandelion flavor increased. The addition at 1% of the dandelion leaves powder content with the dandelion sulggidduk and the addition at 3% of the dandelion roots powder content with the dandelion sulggidduk showed the highest overall acceptability.

급성 관동맥증후군 환자의 신기능 이상과 주요 심장사건과의 관계

이상재,윤경호,최준호,송미진,김연경,박은미,김현정,이은미,유남진,김남호,오석규,정진원 圓光大學校 醫科學硏究所 2006 圓光醫科學 Vol.21 No.1

광주 비엔날레' 특별 세미나 참관기

김미경 한국정신분석학회 1997 精神分析 (Psychoanalysis) Vol.8 No.2

그룹 감각통합치료가 학령전기 발달지연 아동의 감각운동 및 행동 변화에 미치는 효과

김진미,김경미 대한감각통합치료학회 2009 대한감각통합치료학회지 Vol.7 No.2

목적: 본 연구는 그룹 감각통합치료가 학령전기 발달지연 아동의 감각운동 및 행동 변화에 미치는 효과에 대하여 알아보았다. 연구방법: 연구대상은 만 3~5세의 발달지연 아동 5명이었고 실험기간은 2009년 6월에서 11월까지이었다. 그룹 감각통합치료는 주 1회(매 70분) 총 20회기를 실시하였다. 그룹 감각통합치료의 효과를 알아보기 위해 단일집단 사전-사후 설계(one group pretest-posttest design)를 사용하였다. 그룹 감각통합치료 사전/사후의 임상관찰, Behavior during testing의 차이는 윌콕슨 순위검정(Wilcoxon matched-pairs signed ranks test) 을 사용하여 분석하였다. 결과: 그룹 감각통합치료 전과후의 임상관찰(Z = -2.023, p = .043) 점수는 소폭 상승하였으나 통계적으로 유의하지 않았다. 행동 변화를 보기 위한 하위 카테고리인 사회성(Z = -2.121, p = .034), 주의집중(Z = -2.032, p = .042)과 Behavior during testing 총점(Z = -2.032, p = .042)에서 통계적으로 유의한 차이를 보였다. 결론: 본 연구는 그룹 감각통합치료가 감각운동 발달에 긍정적인 영향을 미치나 확실한 근거를 제시하기에는 미흡하며 사회성 및 주의집중과같은 행동 변화에는 효과가 있음을 제시하였다. 이는 개별적인 감각통합치료에 대한 연구는 많으나 그룹 감각통합치료의 효과에 대한 연구는 거의 없는 실정이므로 그룹치료에 대한 근거를 제시하였다는 것에 의의가 있다. 그러나 연구대상자의 수가 적. 고 다양한 내적·환경적 요소에 의해 영향을 받을 수 있으므로 연구결과를 일반화하기에는 어려움이 있다. 따라서 향후 연구에서는 대상 아동의 수를 고려하고 외적변인을 제한하며 보다 객관적인 자료 수집 방법을 사용해야 할 것으로 사료된다.

가사노동의 에너지 대사에 관한 연구 : 작업대 높이를 중심으로 On the basis of the height of Work Surface

김미현,김영숙,신경원,이미선 효성여자대학교 가정대학 학도호국단 1986 家政大論集 Vol.5 No.-

본 연구의 목적은 동일 작업대에서 가사노동시 작업자의 신장과 에너지 대사와의 관계를 분석하는데 있다. 실험은 가사에 경험이 있는 자로서 신장별로 A(152㎝), B(159㎝), C(172㎝)를 피실험자로 하여 Respilyzer를 이용한 간접열량 측정법을 이용하였다. 실험 결과를 요약하면 다음과 같다. 1. 각 작업의 R. M. R. 평균은 설겆이 작업 0.64, 고구마 채썰기 0.77, 고구마 볶기는 0.42였다. 2. 개수대 작업에서 신장별에 따라 R. M. R.은 피실험자 C. A. B.의 순으로 키가 큰 사람이 (C) 1.16으로 에너지소모가 가장 많았다. 3. 준비대 작업에서 신장별에 따라 R. M. R.은 피실험자 A. B. C의 순으로 나타났으나 그 차이가 타작업에 비해 근소하였다. 이상의 결과를 볼 때 현재 시판되고 있는 작업대 높이에서 주부가 가사노동할 경우 표준신장에 가까운 사람에 비해 신장에 차이가 있는 사람은 개수대, 준비대에서의 작업시 에너지소모가 많다는 것을 알 수 있다. 생활수준의 향상과 더불어 신장도 증가하는 일로에 있으나 가사노동하는 주부의 체위는 다양하다. 이러한 점을 고려할 때 기업체에서는 시판 작업대 제작시 높이를 평균 키에 맞추어 단일화시킬 것이 아니라 작업자의 체위를 고려하여 적어도 상, 중, 하의 높이의 형으로라도 되어질 것이 요구된다고 하겠다. The purpose of present research is to analyze the relation of the height of homeworker and the Energy- Metabolism when welted on Sink Center, Mix Center andRange . Experiment was used indirection - calorimetry with Respilyzer and subjects wereclassified into A(152cm), B(158.6cm) and C(172cm) according to height. The results of this study were as follows ; 1. The average of R. M. R. in each homeworking was 0.64 in dishwashing (Sink Center), 0.77 in sweet potato slicing (Mix Center) and 0.42 in sweet potato Panbroiling (Range). 2. In dishwashing, R. H. R. according to height was the highest in the subject Cin order of C.A. B. 3. In sweet potato slicing, R. M. R. according to height was the hightest in thesubject A in order of A. C. B. 4. In sweet potato pan-broiling, R. M. R. according to height was high in orderof the subject A. B. C but the difference of R. M. R. among A. B. C was a few ascompared with dishwashing and sweet potato slicing.

감각통합중재 효과에 관한 체계적 고찰

김경미 대한감각통합치료학회 2009 대한감각통합치료학회지 Vol.7 No.2

목적: 감각통합중재의 효과에 대한 체계적인 고찰을 통하여 감각통합중재의 객관적인 근거기반을 제시하기 위하여 감각통합중재가 어떤 효과가 있는지 알아보고, 중재 결과 측정에 사용된 평가도구에 관하여 알아보고자 한다. 연구방법: 연구방법으로 체계적 고찰을 사용하였다. 사용된 데이터베이스는 MEDLINE/PubMed, PsycINFO, CINAHL, Cochrane Library, OTSeeker 이었고, 검색어는 "effects of sensory integration", "sensory integration therapy"와 "sensory integration intervention"이었다. 최종적으로 분석에 사용된 논문은 11개로, 근거의 질적 수준은 수준Ⅰ과 수준 Ⅱ, Ⅳ이었다. 분석내용으로는 감각 통합중재의 대상군과 중재방법, 중재결과 측정 영역에 따라 중재결과를 측정하는 도구 및 중재효과를 분석하였다. 결과: 1. 감각통합중재 대상으로는 자폐스펙트럼장애가 30.7%로 가장 많았으며, 전반적발달장애와 조산아가 각각 15.4%이었다. 2. 감각통합중재 방법으로는 J. Ayres에 의해 제시된 전통적인 방법이 36.4%로 가장 많이 사용되었고, 감각통합 중재의 효과를 알아보기 위하여 사용된 결과측정 영역은 행동의 문제를 해결하기 위한 영역이 40.63%로 가장 많았으며, 운동수행결과를 보기 위한 것이 28.13%, 감각기초 결과를 보기 위한 것이 25.0%이었다. 3. 감각통합중재 효과는 운동수행기술의 중재결과가 가장 많았으며(66.7%), 그 다음으로는 감각기초를 위한 중재결과가 62.5%, 그리고 행동 문제 해결에 효과적인 경우가 61.5% 순으로 분석 되었다. 학습과 관련하여 쓰기기술에는 효과적이지 않은 것으로 나타났다. 결론: 감각통합중재는 운동수행기술과 감각기초능력 향상, 그리고 행동문제의 해결에 도움을 제공할 수 있는 치료적 접근방법이었으나 학습에 관하여는 효과적이지 않았다. 앞으로 감각통합중재의 일차적인 효과가 지속되어 학습이나 일상생활에까지 영향을 미칠 수 있는가에 대한 연구문제를 가지고 연구방법 및 평가도구 중재방법 등에 관한 연구가 필요할 것으로 생각된다. Objective : The purpose of this study was to provide evidence for the effectiveness of Sensory Integrative Intervention (SII) through a systematic review. Method : The systematic review was executed using MEDLINE/PubMed, PsycINFO, CINAHL, Cochrane Library, OTSeeker. The key words for search was “effects of sensory integration”, “sensory integration therapy”, and “sensory integration intervention”. 11 studies were used for data analysis, and the level of evidence were level Ⅰ, level Ⅱ and level Ⅳ. The substances of review were subject (population), meth ­od (intervention type), effect of the intervention, outcome parameter, and outcome measurement. Results : 1. The subjects of SII were autism spectrum disorders (30.7%), pervasive developmental disorder (15.4%) and preterm infants (15.4%). 2. Most frequently used method was the traditional intervention of J. Ayres (36.5%). The outcome parameters of SII were behavioral outcome (40.63%), motor perform­ance outcome (28.13%), sensory-based outcome (25.0%), and academic outcome (6.35%). 3. The effects of SII were improvement of motor performance (66.7%), sensory-based (62.5%), and behavior problems (61.5%). In most studies, however, academic skill such as writing skill was not a positive outcome pa­rameter in terms of effectiveness of SII. Conclusions : The results implied that the sensory integrative intervention is effective on motor performance, sensory-based and behavioral change, but not on the academic skill. It is suggested that the future re­search need to be done to examine whether and how the effect of sensory integration intervention can be long-lasting and contribute any academic skill and activities of daily living performance.