김치에서 분리한 Lactobacillus sp. JC-7과 Lactobacillus acidophilus 88간의 Electrofusion 최적조건 설정

조영배,최현정,백형석,전홍기 부산대학교 유전공학연구소 1997 분자생물학 연구보 Vol.13 No.-

김치 발효 숙성기간을 연장하고 신선도를 오랫동안 유지 할 수 있는 김치발효 starter의 개발을 목적으로 최적숙성기에 있는 김치로부터 bacteriocin생성능이 없는 유산균을 분리하여 동정하였으며, bacteriocin생성능이 우수한 L.acidophilus 88을 융합시키기 위해 electrofusion에 대한 최적 조건을 검토하였다. 분리균주는 Lactobacillus속으로 동정되어 Lactobacillus sp. JC-7이라 명명하였다. Electrofusion에 의해 생성된 융합주를 식별하기 위해 streptomycin(2.5㎎/ml)에 내성을 나타내는 Lactobacillus sp. JC-7변이주와 kanamycin(600㎍/ml)에 내성을 나타내는 L.acidophilus 88변이주를 분리하였다. Electrofusion을 100V/㎝, 120msec(72ohms,1670 capacitance) 에서 수행했을 때 융합효율이 가장 양호하였으며 전기장의 세기와 시간이 중가할수록 융합 효율이 현저히 감소하였다. 2가 양이온은 농도가 중가할수록 대체적으로 융합효율을 감소시키는 경향을 나타내었으나 1mM MGCl_2에서는 대조군에 비해 융합효율이 약간 증가하였다. PEG매개에 의한 융합법의 융합효율을 비교한 결과, 융합효율은 chemical fusion<electrofusion<electrofusion+20% PEG순을 나타내었다. Evaluation of Optimum Conditions for the Electrofusion between Lactobacillus sp. JC-7 Isolated from Kimchi and Lactobacillus acidophilus 88. Young-Bae Jo, Hyun-Jung Choi, Hyung-Suk Baik and Hong-Ki Jun*. Department of Microbiology, Pusan National University, Pusan 609-735,Korea-A lactic acid bacterium was isolated from kimchi. The isolated strain was identified as the genus Lactobacillus thrugh its morphological characteristics and named as Lactobacillus sp. JC-7. The optimum conditions for the electrofusion between streptomycin(2.5㎎/ml)resistant mutant of Lactobacillus acidophilus 88 and kanamycin(600㎍/ml) resistant mutant Lactobacillus sp. JC-7 were evaluated. The highest number of fusants were obtained at a capacitance value of 120msec(1670㎌), a field strength of 100V/㎝,and a pulse controller setting of 72Ω. The potimum pH of elecroporation buffer was 7.5 and the concentration of divalent cation was 1mM MG^2+. Electrofusants were efficiently obtained by addition 20% polyethylene glycol to electroporation buffer. The yield of fusion was better than that of using polyethylene glycol mediated chemical induction.

개에 있어서 심장사상충증을 동반한 창상성 횡격막 허니아의 수술적 치료 1례

조승혁,임영환,손정민,노경완,박창식,전무형,김명철,송근호,정성목 忠南大學校 獸醫科大學 動物醫科學硏究所 2005 動物醫科學硏究誌 Vol.13 No.-

A 7-month old, castrated male Shih-tzu dog was referred to the Veterinary Medical Teaching Hospital of Chungnam National University with history of depression, dyspnea, mild scale, tachypnea, exercise intolerance and severe leanness. On physical examination, cardiac arrhythmias was heard and heart worm ELISA test was positive. Microfilaria was found on the direct blood smear. Survey radiographs showed loss of diaphragmatic line and cardiac silhouette, displacement of lung fields and presence of gas filled intestines in the thoracic cavity. Rib fracture and pelvic fracture with callus formation were found incidentally. On the basis of physical examination and radiographic findings, it was diagnosed as traumatic diaphragmatic hernia and heart worm disease. Herniorrhaphy was performed. Postoperatively, it was treated for heart worm disease and scale. There is no evidence of recurrence of diaphragmatic hernia and heart worm disease over 1 year.

복강내에 발생한 다발성 혈관종 : 증례 보고

정갑중,김형호,최홍조,김영훈,조세헌,정진숙,남경진,김상순 동아대학교의과대학 1992 동아의대학술지 Vol.4 No.1

개에 있어서 심장사상충증을 동반한 창상성 횡격막 허니아의 수술적 치료 1례

조승혁,임영환,손정민,노경완,박창식,전무형,김명철,송근호,정성목 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10

A 7-month old. castrated male Shih-tzu dog was referred to the Veterinary Medical Teaching Hospital of Chungnam National University with history of depression. dyspnea. mild scale. tachypnea. exercise intolerance and severe leanness. On physical examination. cardiac arrhythmias was heard and heart worm ELISA test was positive. Microfilaria was found on the direct blood smear. Survey radiographs showed loss of diaphragmatic line and cardiac silhouette. displacement of lung fields and presence of gas filled intestines in the thoracic cavity. Rib fracture and pelvic fracture with callus formation were found incidentally. On the basis of physical examination and radiographic findings. it was diagnosed as traumatic diaphragmatic hernia and heart worm disease. Herniorrhaphy was performed. Postoperatively. it was treated for heart worm disease and scale. There is no evidence of recurrence of diaphragmatic hernia and heart worm disease over 1 year.

Alginate 배양내에서 Transglutaminase가 연골막세포에 미치는 영향

박정선,공차배,김충현,안현정,정미현,성상철,김희중,조현철,이상훈,이명철 대한정형외과연구학회 2003 대한정형외과연구학회지 Vol.6 No.2

보신 관광에서 감염된 수입성 Sparganosis 1예 : Intake of Thailand Snake during Trip

서환조,박명재,고경식,백영한,조유정,양현종,류경남,정형근,전정열 대한감염학회 1992 감염 Vol.24 No.1

Human sparganosois was reported more than 100 cases in Korean population. However no case reported yet in Korea as a patient was infected by sparganum from other geographical area, such as Thailand. The case we are reported here is a 38 year-old Korean male who had been suffered from mass being a small, round and soft consistance in upper portion of his right thigh. The patients stated that he had an opportunity to eat living snake in Bangkok, Thailand during trip. As therapy praziquantel (75㎎/㎏/day) was given p.o. for 14 days, but in vain to cure. Following medical treatment, surgical exicision was performed to remove a alive, soft, whitish-milky, mobile and elongated sparganum. Serlogical test by micro-ELISA for sparganum-specific antibody revealed a high serum antibody level of absorbance 0.67 (positive criterion: absorbance 0.22).

고분자 복합재료를 위한 액정중합체(I)

조철형,조성휴,조병욱,김정규,김숭평,권중근 조선대학교 생산기술연구소 1983 生産技術硏究 Vol.1983 No.-

Copolyesters containing oxybenzoate as a rigid rod molecule and methylene group and dimethyl tetramethyl disiloxane as flexible spacers were prepared by room temperature solution polymerization for the purpose of the developement of polymeric composite materials. The thermal properties and the characteristics of the polymers were investigated by DSC and a polarizing microscope. The polymers may be formed nematic liquid crystal phase, and the thermodynamic characteristics of their mesophase to isotropic phase transition could be explained on the basis of its structural features.

담양신설 IC의 교통소음도 조사연구

최형일,강영주,채희정,김연풍,조도호,설동화,정숙경 조선대학교 환경공해연구소 2007 環境公害硏究 Vol.20 No.1

After the vehicle has become a large formation with high-speed, loud noise from an express highway is increasing, and the number of large-scale apartment complexes, which are recently being constructed in the metropolitan area nearby the express highway and IC, is increasing gradually. Compared with a main road, the traffic volume and the speed of a vehicle between IC and the ramp way [turn-off] are smaller. If the ramp way is rightly connected with the downtown area, however, the traffic-noise that comes about in surrounding areas will be in conjunction with the traffic-noise from the main road of express highway and that of the ramp way. This research has selected a place that hasn't had a road or a commercial quarter nearby the main-road of IC and ramp way. Then we have studied the traffic-noise status under minimizing influence of its outer-noise, which occurs in IC. 1. The traffic volume change of a small and full-sized car with time has been kept on regularity in 2004 and 2005. As the traffic volume of a full-sized car has diminished remarkably, it has been maintaining through 6:00a.m.-10:00 a.m. As it has decreased since lO:OO a.m.. we have also observed the traffic proportion going up. 2. Monthly traffic volume of small cars was higher in May, August and October than other months. We have observed that the high traffic volume during these months is due to the following: May-The Damyang festival, August-a high-demand season, October-viewing scarlet maple leaves. Monthly traffic volume of full-sized cars was generally higher in August, but it has decreased during 2006. This was due to the many construction trucks that were working on the extension of the 88 Olympic expressway. Therefore, there was more traffic volume in 2006 compared to 2004 and 2005. 3. In this study, the noise figures have been less than 55 dB(A) in 18 spots. There were 14 spots from 55dB(A) to 58dB(A). 58-61dB(A) : 20, 61-64dB(A) : 17, 64-67dB(A) : 18. There were 8 spots we observed at the highest level from 67dB(A) to 70dB(A). Those were the nearby spots separating the main road and Damyang IC. 4. Traffic-noise at the boundary between IC and the main road of an expressway was higher than that of the same distance in other places. 5. We have observed that traffic-noise is low in places with mountains that serve the role of soundproofing walls. Thus, the fundamental sound hadn't affected it.

정상 관동맥을 가진 심장 점액종 환자에서 발생한 급성 심근 경색증 1예

이정민,이현승,전은정,윤호중,정욱성,오용석,김철민,김재형,최규보,홍순조,이교영,심상인 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.9

도로교통소음의 수평·수직적 전달 및 감쇠 특성

최형일,채희정,김연풍,조도호,설동화,정숙경,김정애 조선대학교 생산기술연구소 2007 生産技術硏究 Vol.29 No.1

This study has been conducted to achieve the following objectives: First, in order to understand the horizontal propagation and attenuation characteristics for the road traffic noise, we selected actively developed areas within 100 meters away from the road to measure the level of traffic noise near a road. To measure the noise near a railroad, we selected areas within 100 meters away from the road. In this way, we analyzed the horizontal propagation and attenuation characteristics for the traffic noise occurring in diversified areas. Second, in order to understand the vertical propagation and attenuation characteristics for the road traffic noise, we measured and analyzed the distributional characteristics of vertical sound pressure levels on each floor of multi-storied apartment buildings according to changes of traffic load and types, and the existence or nonexistence of soundproof walls. For the case of the road traffic noise, we also selected we measured and analyzed the different noise levels on each floor of multi-storied apartment buildings from the soundproof wall. The results of Horizontal propagation and attenuation characteristics for the road traffic noise are as follows: In cases of the soundproof wall + raising ground, raising ground, cutting land, and flat land as distance increases, the sound pressure level steadily decreases. The soundproof wall + raising ground has an especially high distance decrease compared to the other areas, and the sound pressure level for the raising ground is higher than that of the cutting land with a measurement of 10.7~12.1dB(A). Vertical propagation and attenuation characteristics for the road traffic noise are as follows: The amount of sound pressure level decrease is 12.6- 14.5dB(A) for first floor when measuring the vertical sound pressure levels at soundproof wall, which indicates a fairly large decrease. At fifth and sixth floor, the amount of decrease is 2.3dB(A), which indicates a small decrease.