경안면 괴사성 근막염 : 증례보고

박관수,정기훈,김효언,정정권,윤규호,전인성 대한악안면성형재건외과학회 2001 Maxillofacial Plastic Reconstructive Surgery Vol.23 No.1

Necrotizing fasciitis is defined as infectious disease showing extensive necrosis of the superficial fascia with widespread involvement of the surrounding tissues and concurrent systemic toxicity. It is found commonly in the extremities, the trunk and the perineum, but shows extremely rare occurrence in the well vascularized area such as head and neck area. Occurred in the head and neck area, it is called "cervicofacial necrotizing fasciitis" (CFNF). It is a fatal disease which carries a mortality rate up to 60%. Early diagnosis, aggressive surgical debridement of necrotic tissues and massive antibiotics therapy are essential for achieving a favorable outcome. We present a case of cervicofacial necrotizing fasciitis in 35-year-old male patient with literature review.

E. coli 과량발현을 통한 B형 간염 바이러스 preS1 유도체의 안정한 동위원소 N의 치환

박병관,김세하,한규훈,김선영,홍요정,이시형,한동설,김순종,박진구 木浦大學校基礎科學硏究所 1997 基礎科學硏究誌 Vol.15 No.-

We have used molecular biology technique to label the side chains and backbone of HBV preS1(1-56) with stable isotope (??) during E. coli over-expression and purified the protein to near homogeneity. The expression level of ?? labelled preS1(1-56) seems to be ∼15% of the total protein. to characterize the secondary structure of the labelled protein, circular dichroism was used. In different buffer conditions including pH 3.3 and pH 7.4 the protein seems to be devoid of any stable secondary structure probably suggesting that the N-terminal portion (preS1(1-56)) of HBV preS1 might be unstructured before binding to its target cells.

들잔디 성숙종자로부터 캘러스배양 및 식물체 재분화에 미치는 몇 가지 요인의 영향

이상훈,김범수,원성혜,조진기,김기용,박근제,성병렬,이효신,이병현 Plant molecular biology and biotechnology research 2004 Plant molecular biology and biotechnology research Vol.2004 No.-

In an effort to optimize tissue culture responses of zoysiagrass (Zoysia japonica Steud.) for genetic transformation, factors affecting callus induction and plant regeneration were investigated. MS medium containing 3 ㎎/L 2,4-D was optimal for embryogenic callus induction from mature seed. The plant regeneration frequency of 73.3% was observed when embryogenic calli induced in this medium were transferred to N6 medium supplemented with 0.1 ㎎/L 2,4-D and 5 ㎎/L BA. Among several basic media, MS and N6 medium were optimal for callus induction and plant regeneration, respectively. Regenerated plants were grown normally when shoots transplanted to the soil. A rapid and efficient plant regeneration system established in this study will be useful for molecular breeding of turfgrass through genetic transformation.

Methylosinus trichosporium OB3b에서 세포내 메탄자화효소의 활성 측정

송효학,김경태,박성훈,강정미 釜山大學校生産技術硏究所 1996 生産技術硏究所論文集 Vol.50 No.-

메탄자화균을 이용한 지방족 염소화합물의 분해를 위해 메탄자화균의 일종인 Metlylosirus trichosporium OB3b를 배양하고 메탄자화효소의 활성을 5ml vial을 이용하여 여러조건에서 측정하였다. 기절로 propene을 사용하고 생성물인 propene oxide를 가스크로마토그래프(GC)로 분석하였다. 환원력 공급제로 formate가 첨가될 경우 그렇지 않은 경우에 비해 MMO의 활성이 4-5배 가량 높았고, 최적 pH와 온도는 각각 pH 7.0-7.5, 30℃였다. 기질에 대한 포화상수 Km의 값은 ?????mol/L였고 고농도 propene oxide는 활성을 저해하였다. 균체량이 너무 적으면 GC에서의 분석감도에 문제가 있었고 너무 많으면 산소전달이 제한되므로 MMO 활성 측정값이 저하되었다. 분석에 적합한 최적 균체량은 MMO 활성에 따라 달라지지만 대략 0.3∼0.8㎎/ml 정도였다. In order to determine the optimal assay conditions, the activity of the whole-cell methane monooxygenase(MMO) in Methylosinus trichosporium OB3b was measured under various conditions using gas chromatograph(GC). The enzymatic reaction monitored was propene oxidation to propene oxide and the reaction container was 5ml vial with a PTFE-faced rubber septum. When fermate was added as reducing agent, MMO activity was consistently 4-5 times higher compared to the case that fermate was missing. The optimal pH and temperature were 7.0-7.5 and 30℃, respectively. The dependence of enzymatic activity on propene concentration followed Michaelis-Menten kinetic model and the saturation constant(Km) was observed to be ?????mol/L The enzymatic activity was reduced with increasing incubation time or by high concentration of propene oxide. Considering the sensitivity of GC measurement and mass transfer resistance of gas substrates, proper cell density in the present assay system was estimated to be 0.3-0.8㎎/ml.

비소세포폐암에서 Microsatellite Instability

전효성,김정란,손지웅,박선하,박태인,김창호,김인산,정태훈,박재용 경북대학교 병원 2001 경북대학교병원의학연구소논문집 Vol.5 No.1

연구베경:MMR 유전자의 불활성화에 의해 야기되는 뮤전적 불안정성은 발암기전의 한 부류로 인정되고 있다.저자들은 비소세포폐암의 발암과정에서의 MSI의 역할을 규명하기 위해 비소세포폐암에서 MSI의 빈도 및 MSI 유무에 따른 임상상의 차이를 조사하였다. 대상 및 방법:근치적 절제술을 받은 비소세포폐암 20예를 대상으로 하였다.동결된 폐암조직과 환자의 림프구에서 DNA를 추출한 후 3P와 9p의 15개의 marker들을 대상으로 PCR을 시행하고 7% polyacrylamide gel에서 전기영동한 후 silver 염색을 시행하였다. 암조직과 림프구 DNA의 PCR product의 band를 비교하여 MSI와 LOH를 판정하였다. 결과:1)대상환자들은 남자 19예,여자 1예였으며 모두 흡연자였고 평균 흠연력은 47 갑년이었다. 폐암의 조직형은 편평상피암 15예, 선암 4예,대세포암 1예였고, 술 후 병리학적 병기는 Ⅰ기 6예,Ⅱ기 5예, ⅢA기 7예,ⅢB기 2예였다. 2)20예 가운데 8예(40%)에서 MSI가 관찰되었으며 3예는 한 개의 marker에서,5예는 2개 이상의 marker에서 MSI가 관찰되었다. 3)LOH는 10예(50%)에서 있었으며,LOH유무에 따른 병가 및 흡연력의 차이가 없었다. 4)분석한 marker의 10% 이상에서 MSI가 관찰된 MSI-L종양은 5예였으며, 대부분의 marker에서 MSI양성인 MSI-H종양은 없었다.MSS종양과 MSI-L종양은 흡연력, 병기, 폐암 조직형 및 LOH 빈도의 유의한 차이가 없었다. 결론:비소세포폐암에서 MSI는 비교적 흔히 관찰되지만 MMR 유전자의 불활성화에 의한 MMP pathway 는 비소세포폐암의 주요 발생기전은 아닐 것으로 생각된다.향 후 비소세포폐암의 발암과정에 있어서 MMP pathway의 역할을 규명하기 위해서는 보다 많은 예를 대상으로 한 연구가 필요할 것으로 생각되며, MSI 발생기전에 관한 추가적인 연구가 필요할 것으로 생각된다. Purpose: Microsatellite instability(MSI) is frequently used as an indicator of microsatellite mutator phenotype (MMP) tumors.MSI has been observed in a percentage of non-small cell lung cancer(NSCLC).However, its role in tumorigenesis of NSCLC remains unknown.The frequency and partten of MSI in NSCLC were evaluted and clinical parameters of MSI-positive tumors with those of MSS(microsatellite stable) tumors were compared. Materials and Methods: Twenty surgically resected NSCLCs were analyzed for 15 microsatellite markers located at chromosomes 3p and 9p.The peripheral blood lymphocytes of patients were used as the source of the normal DNA. Results:1) of 20 cases, 8(40%) demonstrated MSI. 2) Instability was observed more frequently in tri- and tetra-nucleotide repeats that in dinucleotide repeats. In all cases, instability appeared as a shift of individual allelic bands. 3) LOH was observed in 10(50%) of 20 tumors analyzed. 4) Of 20 cases, MSI-H tumor(showing MSI in the majority of markers) was absent. There were 5 MSI-L tumors( showing MSI in a greater than 10% of markers). 5) No significant difference was observed between MSI-L tumors and MSI negative tumors in terms of clinicopathologic features such as pack-year history of smoking, histologic subtype, and (delete) stage of disease.There was also no significant difference in the incidence of LOH in relation to the status of MSI. Conclusion: These data strongly suggest that MSI plays different roles in lung and colon cancer.MMP pathway appears to be far less important in the tumorigenesis of NSCLC, caused mainly by cigarette smoke, with little familial tendency.(tuberculosis and Respiratory Diseases 2000,48:24-32)

악성 성상세포종의 수술 및 방사선의 병합 치료에 대한 임상적 고찰

김상훈,이승재,백민우,박효일 최신의학사 1982 最新醫學 Vol.25 No.2

사하구 대기오염물질 배출원 자료체계 구축에 관한 연구

조효정,정장표,박명호,이승훈 慶星大學校 環境問題硏究所 1996 環境硏報 Vol.6 No.1

The air quality has been deteriorated dux to the rapid industrialization and the increase of traffic volume. Especially, the concentrations of air pollutants of Saha-Gu including Shinp'yong-Jangrim industrial complex showed severe air pollution level compared to other districts in Pusan. To establish proper and comprehensive cnntrol strategies for the reduction of air pollutant levels, the information on the emission quantities of air pollutants are necessarily needed. Although there are a few data on the emissions of air pollutants of various sourxes, the data have not been used enough dux to the deficiency of quality and content. Therefore, it is nexessary to establesh the data system of emission inventory for the purpose of being used in air quality management. In this study, the database program of emissions inventory was made using the clipper version 5.2 program. The major functions of datavase program are to estimate the emission quantities and contributions of air pollutants by the grid, the source scale, the source type, and the season in Saha-Gu.

기업의 기부·접대비 지출행위에 관한 연구

홍효석,김동훈,박용철 진주산업대학교 1999 산업과학기술연구소보 Vol.- No.6

This study is to analyze the relation factors of corporate contribution and entertainment expenses. We try to seek to find out the motives of corporate contributions and entertainment expense for describing factors. Previous study is reported different result for our study. Then this study reexamines about this more analytical approach. We select 2,062 sample firms during 1991-1996 and result using correlation analysis is as follows. Contribution and entertainment expense shows a significantly positive with firm size that is corporate sales except for 1991. The contribution and entertainment expense has a significant positive relation with cash flow, industry contribution level, pre-period contribution level, advertisement, research and development cost, effective tax rate, retained earnings. In the case of debt ratio, the result shows significantly negative relation. Therefore we assert that the contribution and entertainment expenses are made for earnings management.

한국인 성인 남성 폐쇄성수면무호흡 환자의 측모 두부 방사선계측학적 비교

황상희,박인숙,남기영,김종배,조용원,서영성,안병훈,박신구,박효상 대한치과교정학회 2008 대한치과교정학회지 Vol.38 No.3

본 연구는 비만도에 따른 한국인 남성 폐쇄성수면무호흡 환자의 측모 두부 방사선계측학적 특성을 파악하기 위하여 시행되었다. 이를 위하여 계명대학교 의과대학 동산의료원 수면클리닉에 수면장애를 주소로 내원하여 수면다원검사 후 치과에서 측모 두부방사선계측사진 촬영을 한 87명의 성인 환자들을 체질량지수(BMI)와 수면무호흡지수(AHI)에 따라 비비만 단순코골이군(Non-obese, simple snorers), 비만 단순코골이군(Obese, simple snorers), 비비만 수면 무호흡군(Non-obese, OSA patients), 비만 수면무호흡군(Obese, OSA patients)의 4군으로 나누어 비교하였다. 그결과, 4군 중 비만 수면무호흡군의 수면무호흡지수가 가장 컸으며, 비만 수면무호흡군보다 비비만 수면무호흡군의 하악각이 더 크고 혀 길이는 더 작았다. 또한, 비비만 수면무호흡군보다 비만수면무호흡군의 설골이 더 전하방에 위치하였고, 수면무호흡지수에 영향을 미치는 기여 인자는 비만 수면무호흡군에서는 혀 길이, 비비만 수면무호흡군에서는 설골의 후방위치였다. 이처럼 비만 수면무호흡 환자와 비비만 수면무호흡 환자의 측모 두부방사선계측학적 특성과 기여 인자가 다르게 나타나므로, 치료방법도 따라서 다르게 선택해야 할 것이다. 비만 수면무호흡 환자들에게는 먼저 체중감량이 권고되어야 할 것이고, 비비만 수면무호흡 환자들은 폐쇄부위에 따라 구강 내 장치나 Nasal CPAP (continuous positive airway pressure), UPPP (uvulopalatopharyngoplasty) 등이 추천될 수 있을 것이다. Objective: The purpose of this study was to compare the cephalometric measurements of obese and non-obese Korean male patients with obstructive sleep apnea syndrome (OSA). Methods: Eighty-seven adults who had visited the Sleep Disorder Clinic Center in Keimyung University, Daegu, Korea were examined and evaluated with polysomnography (PSG) and lateral cephalogram. They were divided into 4 groups (non-obese simple snorers, obese simple snorers, non-obese OSA patients, obese OSA patients) according to AHI (Apnea-Hypopnea Index) and BMI (Body Mass Index). Results: The obese OSA group had the highest AHI among the 4 groups. The non-obese OSA group had a significantly steeper mandibular angle and shorter tongue length than the obese OSA group. The hyoid bone of the obese OSA group was positioned anterior and inferior as compared with the non-obese OSA group. Multiple regression analysis showed that tongue length in the obese OSA group and retroposition of hyoid bone in the non-obese OSA group were significant determinants for the severity of AHI. Conclusions: From a cephalometric point of view, the obese and non-obese pateints with OSA may be characterized by different pathogeneses. Therefore, they have to be managed by individualized treatment. For the obese OSA patients, weight control must be advised as a first choice and for the non-obese OSA patients, oral appliance, nasal CPAP, UPPP and others could be chosen according to the obstructive sites.

진주산업대학교 교육원가 분석에 관한 연구

허익구,홍효석,김동훈,박용철 진주산업대학교 2000 論文集 Vol.39 No.-

Currently the need of educational service is being increased. An effort is needed to cope with dynamic environment. But It is not easy for the national university to assist the finance. The purpose of this study analyze to educational cost for the Chinju national university in 1999. The result supplies an useful information of the decision making of management in chinju national university. The range of our study is limited in calculating and analyzing the educational cost except for the post-graduate course and consignment.