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박재용,임민규,오영규,박재용,한석영,Park, Jae-Yong,Im, Min-Kyu,Oh, Young-Kyu,Park, Jae-Yong,Han, Seog-Young 한국생산제조학회 2010 한국생산제조학회지 Vol.25 No.5
Reliability-based topology optimization (RBTO) is to get an optimal topology satisfying uncertainties of design variables. In this study, reliability-based topology optimization method is applied to the inner reinforcement of vehicle's hood based on BESO. A multi-objective topology optimization technique was implemented to obtain optimal topology of the inner reinforcement of the hood. considering the static stiffness of bending and torsion as well as natural frequency. Performance measure approach (PMA), which has probabilistic constraints that are formulated in terms of the reliability index, is adopted to evaluate the probabilistic constraints. To evaluate the obtained optimal topology by RBTO, it is compared with that of DTO of the inner reinforcement of the hood. It is found that the more suitable topology is obtained through RBTO than DTO even though the final volume of RBTO is a little bit larger than that of DTO. From the result, multiobjective optimization technique based on the BESO can be applied very effectively in topology optimization for vehicle's hood reinforcement considering the static stiffness of bending and torsion as well as natural frequency.
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박재용,황승민,임민규,오영규,박재용,한석영 한국공작기계학회 2009 한국공작기계학회 춘계학술대회논문집 Vol.2009 No.-
This paper presents a reliability-based topology optimization (RBTO) using bi-directional evolutionary structural optimization (BESO). An actual design involves uncertain conditions such as material property, operational load and dimensional variation. Deterministic topology optimization (DTO) is obtained without considering of uncertainties related to the uncertainty parameters. However, the RBTO can consider the uncertainty variables because it has the probabilistic constraints. In this paper, the reliability index approach (RIA) is adopted to evaluate the probabilistic constraint. RBTO based on BESO starting from various design domains produces a similar optimal topology each other. Numerical examples are presented to compare the DTO with the RBTO.
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Streptococcus thermophilus의 자연적 형질전환 메커니즘과 <br>Leuconostoc 속 세균에서 그 메커니즘의 추론
박재용 한국유산균·프로바이오틱스학회 2013 Current Topic in Lactic Acid Bacteria and Probioti Vol.1 No.1
Natural genetic transformation is one of mechanism for horizontal transfer of genetic elements in bacteria which is an integral part of the physiology of bacteria. Streptococcus thermophilus utilize a novel quorum sensing system to control for development of natural competence which composes Rgg-type response regulator (ComR) and hydrophobic peptide pheromone (ComS). Matured ComS is released outside of cell during early exponential phase when growing in peptide free chemical defined medium (CDM). Matured ComS is transported via Ami (oligopeptide transport system) form outside to cytoplasm, and then bind to ComR. Activated ComR (matured ComS combining form of ComR) induces transcription of comX, which encodes an alternative sigma factor (σ<sup>x</sup>) that regulates expression of late competence genes. Adding synthetic matured form of ComS to CDM, it can induce genetic transformation of S. thermophilus. The genus Leuconostoc has not been identified natural transformation mechanism even though presenting homologous genes related with late competence development. The study for identifying natural transformation mechanism of Leuconostoc can realize development of improved strains for kimchi industry.
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