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      • SCOPUSKCI등재

        Quantitative Analysis of Weissella cibaria against Periodontopathic Bacteria by Real-time PCR

        Mi-Sun,Kang,Hoi-Soon,Lim,Seon-Mi,Kim,You-Jin,Lim,Hyun-Chul,Lee,Jong-Suk,Oh 대한미생물학회 2009 Journal of Bacteriology and Virology Vol.39 No.4

        The objective of this study was to analyze quantitatively whether Weissella cibaria could affect the proliferation of five periodontopathic bacteria, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Aggregatibacter actinomycetemcomitans, and Fusobacterium nucleatum, after incubation for 8~48 h. In addition, by using real-time PCR with a dual-labeled probe, each growth of bacteria was examined under different growth media conditions. The proliferation of periodontopathic bacteria was significantly inhibited by W. cibaria after incubation for 24~48 h (p < 0.05), whereas the growth of W. cibaria was not affected by these pathogenic bacteria. The growth of P. gingivalis, T. forsythia and T. denticola significantly increased in each growth media after incubation for 24 h (p < 0.05), as compared to the culture in mixed growth media. However, no differences in the growth of five periodontopathic bacteria were observed between each growth media and mixed media after incubation for 48 h. The growth and pH of W. cibaria culture significantly were changed in MRS after incubation for 24~48 h (p < 0.05), as compared to the bacterial culture in mixed growth media. The pH of P. gingivalis and F. nucleatum culture significantly was changed in both growth media and mixed media after incubation for 24~48 h (p < 0.05). Our data indicate that W. cibaria significantly inhibits the proliferation of five periodontopathic bacteria and each growth of bacteria is quantitatively analyzed under various media conditions by real-time PCR.

      • SCOPUSKCI등재

        Prevention of Mold Growth on CCA-treated Radiata Pine Lumber by Incorporation of Moldicide into the CCA Solution

        Kang,,Sun-Mi,Kim,,Gyu-Hyeok The Korean Society of Wood Science Technology 2007 목재공학 Vol.35 No.2

        Surface mold fungi growing on CCA-treated wood could be inhibited effectively by the incorporation of moldicide into treating solution. In this study, moldicides compatible with the CCA solutions from various commercial moldicides were screened, and then their optimum concentrations for controlling surface mold on CCA-treated radiata pine sapwood were examined through both the laboratory and the field trials. Among nine commercial moldicdes tested, two substituted isothiazolinones, moldicide A containing 2-n-octyl-4-isothiazoline-3-one and moldicide B containing 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one, were chemically compatible with CCA solution. The optimum concentration to be incorporated into 2% CCA treating solution was determined to be 0.001% for moldicide A and 0.003% for moldicide B.


        Effect of Leuconustoc spp. on the Formation of Streptococcus mutans Biofilm

        Kang,,Mi-Sun,Kang,,In-Chol,Kim,,Seon-MI,Lee,,Hyun-Chul,Oh,,Jong-Suk The Microbiological Society of Korea 2007 The journal of microbiology Vol.45 No.4

        Insoluble glucans synthesized by Streptococcus mutans enhance the pathogenicity of oral biofilm by promoting the adherence and accumulation of cariogenic bacteria on the surface of the tooth. The objective of this study was to investigate the effect of Leuconostoc spp. on the in vitro formation of S. mutans biofilm. Three strains, Leuconostoc gelidum A TCC 49366, Leuconostoc mesenteroides ssp. cremoris A TCC 19254 and Leuconostoc mesenteroides ssp. mesenteroides ATCC 8293, were used in this study. They exhibited profound inhibitory effects on the formation of S. mutans biofilm and on the proliferation of S. mutans. The water-soluble polymers produced from sucrose were most strongly produced by L. gelidum, followed by L. mesenteroides ssp. cremoris and L. mesenteroides ssp. mesenteroides. The mean wet weights of the artificial biofilm of S. mutans were also significantly reduced as a result of the addition of the water-soluble polymers obtained from Leuconostoc cultures. According to the results of thin-layer chromatographic analysis, the hydrolysates of the water-soluble polymers produced by Leuconostoc were identical to those of dextran T-2000, forming predominately ${\alpha}-(1-6)$ glucose linkages. These results indicate that dextran-producing Leuconostoc strains are able to inhibit the formation of S. mutans biofilm in vitro.

      • Poster Session : PS 0989 ; Ceftriaxone-Induced Acute Pancreatitis in an Adult Patient

        ( Sun Mi Kang ), ( Chang Hyeong Lee ), ( Byung Seok Kim ), ( Joon Seok Park ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

        Acute pancreatitis is extremely rare complication in patients treated with ceftriaxone. It was very rarely reported and we could fi nd only 5 cases at Pubmed. Ceftriaxone may precipitate in the bile, and that may lead formation of biliary sludge. Acute pancreatitis, cholangitis and cholecystitis may caused by biliary sludge. We experienced one case of acute pancreatitis which is developed after administration of ceftriaxone. A 70 year-old male presented to the emergency room with drowsy mentality and fever. He underwent open cholecystectomy 10 years ago. He was diagnosed liver abscess and treated with intravenous ceftriaxone (2g, once a day) and metronidazole (500 mg, three times a day). Initial computed tomography revealed liver abscess and periampullary diverticulum. And there was no dilatation of bile duct and biliary sludge. 16 days after the fi rst administration of ceftriaxone, he appealed dyspepsia and epigastric pain. Follow-up CT demonstrated pancreas swelling, peripancreatic fl uid collection, common bile duct dilatation and biliary sludge at distal common bile duct. Laboratory examination showed elevated serum amylase, lipase (272.2U/L, 279.9U/L) and leukocytosis (mm³). Hepatobiliary ultrasonography was performed and there showed mild dilatation of common bile duct. Because the patient was obese, biliary sludge was not seen on ultrasonography. Ceftriaxone-induced pancreatitis was considered, therefore ceftriaxone was immediately discontinued. 2 days after the discontinuance of ceftriaxone, laboratory examination showed normal serum amylase and lipase and symptom was subsided. 2 weeks after the discontinuance of ceftriaxone, leukocytosis has been normalized. Ceftriaxone-induced acute pancreatitis is extremely rare, but we have to aware of the complication, because sometimes it results in severe pancreatitis.

      • KCI등재

        Isolation and Identification of Lactic Acid Bacteria Inhibiting the Proliferation of Propionibacterium acnes and Staphylococcus epidermidis

        Mi-Sun,Kang,Hyun-Ju,Oh,Hyun-Chul,Lee,Jong-Suk,Oh 대한미생물학회 2009 Journal of Bacteriology and Virology Vol.39 No.1

        Propionibacterium acnes is the most common causative agent of acne. Staphylococcus epidermidis is another major bacterial strain to be found in acne lesions. Two strains of lactic acid bacteria (LAB) were isolated from normal inhabitants of humans, which inhibited the proliferation of P. acnes and S. epidermidis. The growth of P. acnes and S. epidermidis was decreased by 4-log scales after incubation for 24 h with LAB isolates, whereas the growth rate of selected LAB isolates were not affected by these pathogenic bacteria. This antibacterial activity of LAB isolates was related to lactic acids, hydrogen peroxide and bacteriocin-like compound production. Two LAB isolates efficiently adhered to human keratinocytes HaCaT and were identified by API 50 CHL medium kit and 16S rDNA partial sequencing analysis. The similarity of 16S rDNA sequences between one isolate and Lactobacillus salivarius subsp. salicinius was 100%, which suggests that they were L. salivarius subsp. salicinius. On the other hand, 16S rDNA sequence similarity between the other isolate and Lactobacillus fermentum was 99.04%, which indicates that it was L. fermentum. In conclusion, these results demonstrate that the two LAB strains isolated from human body were identified as L. salivarius subsp. salicinius and L. fermentum, which inhibit the proliferation of P. acnes and S. epidermidis.


        Influence of the Electric Field on the Ni-Induced Low-Temperature Crystallization of Amorphous Silicon Films

        Sun-mi,Kang,Duck-kyun,Choi,Jin-yong,Ahn,Young-bae,Kim 한국물리학회 2004 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.44 No.1

        Using a field-aided lateral crystallization technique, we were able to crystallize amorphous silicon films at temperatures in the range of 400 500 C. Amorphous silicon films coated with a 50 A-thick nickel catalyst, except for 120 m patterns, were furnace annealed in an applied electric field of 180 V/cm for 5 hours. A comparison between the field-aided lateral crystallization (FALC) process and the metal-induced lateral crystallization (MILC) process in which an electric field was not applied was also performed on identically prepared samples in order to study the effect of the electric field. The degree of crystallization reached 88 % at a temperature of 500 C in case of the FALC process, and the crystallization velocity of the FALC sample at 500 C was about 70 times faster than that of the MILC sample at the same crystallization temperature. In addition, reasonably fast crystallization was achieved in FALC samples even at 400 C. The activation energies of the FALC process and the MILC process turned out to be 1.47 eV and 1.9 eV, respectively. Such a difference in the activation energy is attributed to the electric-field-enhanced diffusivity of Ni atoms during the crystallization procedure.

      • KCI우수등재
      • SCIE

        SPT4 increases UV-induced mutagenesis in yeast through impaired nucleotide excision repair

        Kang,,Mi-Sun,Yu,,Sung-Lim,Kim,,Ho-Yeol,Lim,,Hyun-Sook,Lee,,Sung-Keun 대한독성유전단백체학회 2013 Molecular & cellular toxicology Vol.9 No.1

        DNA repair is a pivotal mechanism in maintaining genetic integrity and cell fate determination. As unrepaired DNA lesions inhibit transcription, UV-induced damage to transcribed DNA is repaired preferentially versus non-transcribed DNA through transcription-coupled nucleotide excision repair (TCR). Previously, we reported that TCR-related genes serve as transcription elongation factors, and defects of the genes drastically increase mutagenesis. Extensive studies on DNA damage repair have provided key information about the pathways controlling replication across DNA lesions. However, knowledge of the mechanisms dealing with stalled DNA transcription is insufficient. In this study, we demonstrated the requirement for SPT4 in cell growth along with its role in mutagenesis in both the presence and absence of DNA damage. SPT4 appeared to promote transcription elongation across DNA lesions, thereby increasing the cell survival rate in exchange for increased mutagenesis. Further, our results explain the decrease in mutant Huntingtin protein in neuronal cells upon inhibition of Supt4, the mammalian ortholog of yeast Spt4p.

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