Production and Characterization of Enzymes Involved in Chitin Catabolic Cascade from a Bacterial Strain Isolated from Soil

( Ji Hyun Lee ),( Jeong In Lee ),( So Yeon Park ),( Yoo Kyeong Jang ),( You Bin Lim ),( Hye Yoon Lee ),( Young Min Jung ),( Hae Chang Lee ),( Jong Hwa Lee ),( Shaheen Amna ),( Jae Kweon Park ) 한국키틴키토산학회 2019 한국키틴키토산학회지 Vol.24 No.1

The present study was aimed to isolate the bacteria producing chitinolytic enzymes including chitin deacetylase (CDA) from soil origin. About 24 bacteria were screened for their chitinolytic enzymes producing ability on the basis of colloidal chitin. Amongst one of the most potent isolates designated to JI-02 was further selected as the most prominent chitinolytic and CDAlike enzyme (CDA-X) producer based on the ability of deacetylation of an artificial substrate pNP-acetamilide. The maximum production of CDA-X in crude enzyme from JI-02 was observed in the presence of 1% colloidal chitin at 37°C and pH 6.4 after 120 h of incubation. Potent enzyme activity of CDA-X was determined toward pNP-acetamilide, demonstrating that optimal pH, temperature and ionic strength of the CDA-X were assessed to be 7.0, 52°C and 125 mM, respectively. Unexpectedly, Nacetylglucosamine (GlcNAc) was not fully converted by the action of CDA-X to glucosamine (GlcN) under the established conditions. Further study on enzyme activity toward chitin-oligosaccharides consisting of multi-N-acetylglucosamine (GlcNAc)n, n=2-5 may be necessary to elucidate the mode of action which requires the minimum size of (GlcNAc)n. However, our data suggest that CDA-X can convert chitin to chitosan with the maximum yield of approximately 0.08g/L during the fermentation of the strain in the presence of chitin. Furthermore, we found that crude enzyme showed the significant chitosanolytic activity. Taken together, our data suggest that the strain JI-02 may be a potential candidate as a particular strain for better understating of chitin catabolic pathway.

진단 기준에 따른 취학전 어린이의 중증 유아기 우식증 유병률 비교

임경욱,이광희,라지영,이동진,안소연,김지영,송지현,김윤희 大韓小兒齒科學會 2008 大韓小兒齒科學會誌 Vol.35 No.4

연구목적은 중증 유아기 우식증에 대한 두 개의 정의를 진단 기준으로 하였을 때 각 기준에 따른 유병률이 일치하는 정도를알기 위함이었다. 연구대상은 익산시 어린이집 중에서 무작위로 추출한 7개 어린이집의 36개월 이상 71개월 이하 어린이401명 이었다. 치경과 탐침을 사용한 구강검사를 통하여 치아별 우식경험유치면 (dmfs)의 수를 조사하였으며 . 중증 유아기 우식증의 진단 기준은 (1) 상악 전치에 1개 이상의, 와동이 형성된 우식,상실(우식증에 의한), 충전 평활면이 있거나 (2) 우식,상실,충전 치면의 수가 3세는 4개 이상.4세는 5개 이상.5세는 6개 이상인 경우로 하였다. 상악 전치부 우식증에 의한 우식유병률과 우식경험유치면수(dmfs)에 의한 우식유병률은 3세에서 각각 26.7%. 28.9%, 4세에서 각각 32.4%, 30.4%, 5세에서 각각 39.4%, 41.3%, 전체에서 각각 35.4%, 35.9%으로서. 연령별로는 약간의 차이가 있었으나 전체에서는 차이가0.5%로서 거의 일치하였다. 따라서 중증 유아기 우식증의 진단은 두 가지 기준 중에서 어느 하나를 사용하더라도 비슷한 결과가 나을 것이라는 것을 확인할 수 있었다. The purpose of study was to compare the prevalences of severe early childhood cahes(S-ECC) using two different diagnostic criteria. The subjects of this study included 401 preschool children from 36 to 71 months of the randomly selected seven nurseries in Iksan city. Severe early childhood caries was defined as (1) 1 or more cavitated, missing (due to caries), or filled smooth surfaces in primary maxillary anterior teeth or (2) a decayed, missing, or filled score of ≥4 (age 3), ≥5 (age 4), or ≥6 (age 5) surfaces. The prevalence determined by maxillary anterior caries and the prevalence determined by dmfs were 26.7%, 28.9% in 3 years, 32.4%, 30.4% in 4years. 39.4%, 41.3% in 5 years, and 35.4%, 35.9% in all, respectively. The results suggest that the two diag-nostic criteria of the severe early childhood caries yield almost the same results.

모 대학병원 입원환자의 직업력 기록에 관한 조사

임현술,김지용,정철 동국대학교 경주대학 1998 東國論集 Vol.17 No.2

From January 9, to January 27, 1996, we surveyed medical records for the occupational history of 352 admitted patients in university hospital located in Pohang city. Also, we interviewed the study subjects about their occupational histories to search for occupational related cases. The results obtained are as follows. The number of males were 205 cases and the number of females were 147 cases. The age and sex distributions of study subjects were not significant (p>0.05). Internal medicine (50.3%), orthopaedics (19.6%), general surgery (8.8%), neurosurgery (5.7%) and obstetrics & gynecology (3.7%) were the major admitted departments of the study subjects. The records of occupational history by doctors were only 9 cases (2.6%) but by nurses were 197 cases (56.0%). And then, there were no detailed descriptions for occupations by doctors and nurses. We would suspect that 18 cases (5.1%) were related to the occupations, of which 13 cases (3.7%) were in occupational injuries and 5 cases (1.4%) were in occupational diseases. It should be emphasized in medical education that the occupational and environmental histories were thoroughly investigated to make a correct diagnosis and appropriate treatment and that the standard forms for occupational history taking should be developed.

중등 영어교사의 자질에 대한 학생, 교사, 학부모의 인식 비교 연구

임정완,정지현 大邱大學校 師範大學 附設 敎育硏究所 2010 學校敎育硏究 Vol.5 No.2

본 연구는 우리나라와 같은 영어를 외국어로 배우고 가르치는 상황에서 영어교사에게 요구되는 전문성과 자질에 대한 의견이 학생, 학부모와 교사 간에 어떻게 다른지를 탐구해 보는 것이 주된 목적이었 다. 이 연구를 위하여 국내외의 문헌과 선행연구를 통하여 영어교사의 전문성에 대한 자질을 먼저 살펴 보고, 영어교사와 학부모, 그리고 학생집단의 설문 조사를 통하여 세 집단 간의 영어교사의 자질에 대 한 인식을 분산분석(ANOVA)을 활용하여 비교, 검토하여 하였다. 연구 결과로는 교사의 자질로는 크게 5가지 영역으로 도출되었는데, 언어지식, 수업기술, 태도, 상호작용, 성찰이었고, 이에 대한 세 집단의 인식은 각각 영역마다 다르게 나타났고, 이는 교사와 학부모가 기대하는 영어교사상과 학생집단이 기대 하는 영어교사상은 상이하다는 것을 알 수 있었다. 또한 교사의 자질에 해당하는 각 5가지 하위 영역과 나이별, 성별, 근무 연수별, 영어실력별 상관관계가 있는 지를 조사한 결과, 근무 연수와 수업기술에 따 른 차이가 발견되었으며, 나머지 요인들과는 상관관계가 없는 것으로 밝혀졌다. 근무 연수가 15년에서 20년 미만의 교사들이 ‘수업 기술’의 중요성을 가장 많이 인식하고 있는 반면에 근무 연수가 20년 이상 이 된 교사들은 오히려 그 중요성을 가장 낮게 보고 있었다. 본 논문에 참여한 세 집단의 참여자 수가 적어서 이를 일반화하기에는 무리가 따르지만, 세 집단의 의견에 공통점과 차이점이 어느 영역에서 존 재하는 것을 보여준 것으로 의의가 있다고 본다. The purpose of this study is to investigate the quality of a good secondary teacher of English from the perspectives of students, parents, and teachers. A questionnaire with 29 questions was distributed to 60 high school students, 66 parents, and 61 secondary school teachers. The questionnaire consisted of questions about the knowledge of English language, skills related to teaching, the personality of the teacher, skills related to reflection, and interaction skills. The questionnaire was analyzed by the use of SPSS 17.0. ANOVA and frequency analysis. The findings of the study were as follows: first, there was a similarity in the aspect of skills related to teaching and a difference in the aspect of knowledge of English language among teacher, parent, and student groups. The teachers and the parents had the same opinions and attitudes towards the qualities of a good language teacher, whereas the students had different opinions. Second, the correlation between the quality of a teacher and age, sex, and language ability was found to be not existent, whereas the working years of teachers correlated to the skills related to teaching. On the basis of the results of the study, implications on effective English teaching are presented, and further pedagogical implications and future study directions are suggested.

절삭유와 유기용매에 혼합 폭로된 근로자의 피부질환에 관한조사

임현술,김지용,성열오 동국대학교 경주대학 1993 東國論集 Vol.12 No.-

Cutting oils denature keratin, defat the skin and remove water from it, causing dryness, fissures and frank eczematization. Solvents remove the surface lipid film. Therefore the skin barrier is weakened and the absorption of irritant substances are increased. Saturated hydrocarbons from the solvent and paraffin series are stronger irritant than those from the aromatic series. To evaluate the health effects on skin among cutting oil and solvent exposed workers, self-administrative questionnaire survey was performed on 72 exposed workers and 91 non-exposed workers. The dermatologic examination and patch test were performed on the workers with suspected skin diseases. The results are summarized as follows ; The prevalences of some dermatologic disorders, such as primary irritant dermatitis, dyshidrotic eczema, allergic contact dermatitis and folliculitis were significantly higher among exposed group than non-exposed group(P<0.05). The positive rate of patch test among 16 workers were 6.3% for cutting oil, 18.8% for old solvent, 18.8% for new solventⅠ and 6.3% for new solvent Ⅱ. These results suggest that cutting oil and solvent may be a cause of occupational dermatoses among exposed workers.

비소세포 폐암종에서 Epidiermal growth factor receptor(EGFR)의 발현 양상

임석호,이지혜,조현득,오미혜,김의한 순천향의학연구소 2008 Journal of Soonchunhyang Medical Science Vol.14 No.1

배경: 비소세포 폐암종에서 epidermal growth factor receptor (EGFR)의 발현이 예후에 미치는 영향에 대해서는 아직 일치된 결론에 이르지 못하고 있으며, 비소세포 폐암종의 조직 병리학적 소견으로는 종양의 진행 및 예후에 대해 예측이 불가능하다. 이에 저자들은 종양의 EGFR 발현과 임상 및 병리학적 특징과의 관계를 관찰하였다. 재료 및 방법: 1990년부터 2004년까지 순천향 대학교 천안병원에서 수술로 절제된 95명 환자의 포르말린에 고정한 파라핀 포매 조직을 이용하여 EGFR 항체를 이용한 면역조직화착 검사를 시행하였으며 이의 결과를 환자의 임상양상과 병리학적 소견과 비교 분석하였다. 결과: EGFR 발현과 환자의 생존기간을 비롯한 성별, TNM 임상병기, TU댕기, N-병기와는 관련이 없었으나 조직학적 유형중 편평상피암종은 47예중 43예가 양성 반응을 보여 통계학적으로 의미 있는 상관관계를 보였다(p=0.005). 그 외에 성별, 조직학적 유형, TNM 임상병기, T-병기, N-병기중 어떠한 변수도 생존기간과의 연관성을 보이지 않았다. 결론: 비소세포 폐암종에서 EGFR의 발현은 환자의 생존기간 및 임상병리학적 소견과 유의한 상관관계를 보이지 않으므로 예후인자로서의 이용은 현재로선 불가능하여 좀 더 많은 증례를 통한 연구가 필요할 것으로 사료된다. Background: The prognostic role of epidermal growth factor receptor (EGFR) remains controversial in patients with non-small cell lung cancer (NSCLC). Histopathologic findings cannot adequately predict disease progression, so we investigated the reladonship between EGFR and clinicopathologic feature. Methods: Using immunohistochemical techniques, we retrospecdvely analyzed formalin-fixed, paraffin-embedded samples from 95 padents with resected pathological NSCLCs. Then, we correlated these data with padents' clinical outcome and pathologic finding. Results: No reladonship was found between EGFR overexpression and patient survival when the entire cohort was considered. Multivariate factors including gender, histologic type, TNM stage, T stage and N stage were not correlated to poor outcome. Also, no factors revealed correlation with EGFR overexpression except histologic type. Squamous cell carcinoma showed positive reaction in 43 out of 47 patients(p=0.005). Condusion: This result indicated that EGFR overexpression in NSCLC showed no reladonship with patients' survival. Therefore, EGFR overexpression could not be used as a poor prognostic marker.

한국, 미국, 중국 청소년의 의복 행동에 관한 연구

임숙자,이지현,문정미,장춘희 이화여자대학교 생활환경대학 인간생활환경연구소 2002 인간생활환경연구소 논집 Vol.- No.1

The purposes of this study were to determine similarities and diferences in the clothing behavior of Korean, American and Chinese Adolescents. Photographs and questionnaires were conducted for this study and the results are as follows. 1) By analyzing the questionnaire survey, Korean adolescents considered their friends advise more when purchasing clothing while Chinese adolescents depended on their parents. In clothing elements, American adolescents considered attractiveness to other sex more than other two countries. 2) For the Photograph analysis, Many bright and vivid color clothing were seen from Chinese adolescents and dull achromatic colored clothing was noticeable from Koreans. Jeans were favored by all three countries but chino pants were only noticeable from Korean adolescents. Even though it is said 'It is one world ond market', this study shows that it is very important to consider the cultural differences among countries.

놀이학습을 통한 초등 영어교육

임지현 The English Linguistic Science Association of Kore 2000 English Linguistic Science Vol.5 No.-

The purpose of the present article is to provide a good language acquistion as well as fun and relaxation for children through various plays and games. The 7th English Curriculum focused on communicative competence. Primary English Education by performing Games in English Class affects English Language learner's communicative competence. This article presents various making-stuff and game materials for children. However this education has many problems such a lack of materials. This paper holes that children would reinforce their learning in this way. Also, we wish to furnish a lot of playing materials and game information.

석면함유 슬레이트 지붕 물받이 퇴적물 중 석면 섬유 함유율

임지현,한솔민,김현석,신유민,박시은,허정윤,김민영,장봉기 순천향대학교 기초과학연구소 2022 순천향자연과학연구 논문집 Vol.28 No.1,2

This study attempted to determine the degree of asbestos release from the aging slate roof by comparing the asbestos content in the slate roof rain gutter with a colored steel plate (tin plate) over the slate roof. Four slate roof houses located in Haengmok-ri, Asan-si, Chungcheongnam-do, and one house constructed with a colored steel plate on the slate roof were selected to collect the sediment of the roof rain gutter. The asbestos fiber content was calculated by a point counting method using a polarization microscope after pretreatment with conversion treatment and hydrochloric acid treatment. The average asbestos content of the four slate roof rain gutter were 1.89%. However, asbestos was not detected in the Slate covering roof rain gutter, which were constructed on the slate roof. Asbestos fiber content was the highest at 2.89% in the slate roof rain gutter installed in 1976, followed by 2.44% in 1953. From the above results, it is necessary to minimize secondary damage as asbestos fibers released from slate roof houses to the surrounding atmosphere or leaked from slate roofs as rainwater may cause soil pollution and seriously affect residents' health. Although covering with colored steel plates (tin plates) has been shown to prevent the leakage of asbestos fibers to some extent, it is believed that a policy alternative to remove the slate roof as soon as possible is needed to solve the fundamental problem.

신축 공동주택의 VOCs와 알데히드류 성분에 대한 실태조사

임득용,김지현,황인조,김동술 경희대학교 2004 環境硏究 論文集 Vol.13 No.-

In comparison with other studies, in particular, the emission of indoor HCHO in new apartments were very serious and Vacs showed below the Japan's guidelines (260 ㎍/㎥) excepting toluene (324.31 ㎍/㎥). Concentration of volatile organic compounds (VOCs) including aldehydes in indoor air was analyzed at 3 new apartments (at the lower, middle and upper floors of each apartment) during the Spring in 2004. The sample gases of Vacs and aldehydes were collected by Silicon canister (6 L) and 2,4-DNPH cartridges, and the analysis was made by the GC/MSD and PLC. As a result of the analysis, formaldehyde (HCHO) amongst aldehydes was found the highest (874.63 ppb) at the upper floor of C Apt., and the aldehydes' concentrations were S > C > K Apt. each. Also it did not show an apparent variation of characteristics according to floor. The concentration of VOCs was the highest (819.22 ppb) at the upper floor of C Apt. which exhibited in proportion with height.