Constituents Analysis of Amino Acid and Antioxidative Activity from Cultivated Callus and Rhizome in Rhodiola sachalinensis

Song,Won-Seob,Chi,Hyung-Joon,Rim,Yo-Sup,Yoon,Jae-Ho 한국자원식물학회 2002 Plant Resources Vol.5 No.1

The material of Rhodiola sachatinensis collected from an alpine region of the west-northern China. For analysing the effect, 1 used Rhodiola sachatinensis s rhizome and cultivated callus. In EtOAc, BuOH, H₂0separation the plant showed strong antioxidative activity, but not in Hexane. The radical scavenging effect of EtOAc(RC50, 35(g), BuOH(RC50, 43(g), H₂0(RC50, 50(g) fraction and MeOH extract(RC50, 50(g) of the Rhodiola sachatinensis was comparable to that of synthetic antioxidant BHA(RC50, 14(g) and α-Tocopherol(RC50, 12(g). Total amino acid concentration of plant of In nature condition were 18,009ppm, and major components were arginine, glutamic acid, aspartic acid and valine. The ratio of essential/total amino acid on plant of In nature condition was 46.93%. Total amino acid concentration of callus of In vitro condition were 32,435ppm, and major components were valine, histidine, lysine and leucine. The ratio of essential/total amino acid on callus of In vitro condition was 56.07%. was 56.07%.

Efficient Plant Regeneration from Shoot Tip and Young Leaf in Rhodiola sachalinensis A. Bor.

Chi,Hyung-Joon,Yoon,Jae-Ho,Yang,Deok-Chun,Song,Won-Seob 한국자원식물학회 2003 Plant Resources Vol.6 No.3

The shoot tip and young leaf of Rhodiola sachalinensis were cultured to invest the plant growth regulator condition for callus induction, shoot and root regeneration. When the shoot tip was sterilized in 2.0% of NaOCl for 20min., the contamination rate was the lowest. And the survival rate of the culture material was good in carbenicillin 500mg/L treatment group. Callus was obtained from shoot tip and young leaf segments. NAA 0.1-1.0mg/L and 2,4-D 0.1-0.5mg/L alone treatment were shown to have a good response on callus induction from shoot tip culture. In the case of young leaf culture, NAA and 2,4-D 0.1-0.5mg/L alone treatment were good in callus induction. In culturing shoot tip NAA 0.5mg/L and BA 0.5mg/L, NAA1.0mg/L and BA 0.lmg/L combination treatment was good in shoot regeneration. The regenerated shoots were rooted on MS medium supplemented with NAA and BA combination treatment. Especially, NAA 1.0mg/L and BA 0.1mg/L combination treatment was effective for root regeneration.

Mongolian gerbil 심장동맥의 해부학적 연구

송치원,정주영,이미영,김무강,현병화 충남대학교 수의과대학 동물의과학연구소 1998 動物醫科學硏究誌 Vol.6 No.-

The arterial supply to heart of mongolian gerbil has been described. Observations were made by the specimens injected with latex after the flowing through in left ventricle. The results were summaried as follows; 1. A. coronaria arose from the initial portion of Aorta in heart and A. coronaria dextra and sinistus arose in Aortic sinus on 10 specimens. A. coronaria sinistra arose more near boundary of Aortic sinus than A. coronaria dextra. 2. A. coronaria sinistra which arose from Aortic sinus divided into Ramus interventricularis sinistra and Ramus circumflexus sinistra. A. coronaria dextra which arose from Aortic sinus only runed continously as Ramus interventriculares dextra. 3. Ramus interventricular sinistra not reach the heart apex through the sulcus interventricula but stopped in thoracocostal plan extending to under part. 4. R. circumflexus runed theough sulcus coronarius, thereafter it runed from left ventricle to Sulcus interventricularis dorsalis. 5. A. coronaria dextra runed to diaphargm side through sulcus interventricula dorsi thereafter it became Ramus interventricular dextra. It distrubuted left and right ventricle wall on 8 specimens and 2 specimen stopped margo obtusus.

流域特性을 考慮한 流出 모델링에 관한 硏究

金治弘,安元植,韓成大,尹如松 成均館大學校 科學技術硏究所 1985 論文集 Vol.36 No.1

This study have been carried out as a sector of the rainfall-runoff relationship research. First of all, the runoff research has the difficult problem due to non-linearity of hydrological phenomena. In this study, according to the method of runoff separation into components by Hino and Hasebe (1980) is applied. This theory considers sufficiently the physical mechanism of runoff process to separate into the groundwater, interflow, and surfaceflow, respectively. Then each component of runoff time series can be reduced to the linear subsystem, that is, ARMA model can be obtained in each subsystem. Actual flood hydrograph of the every two major gauging stations in the Han River basin, the Naktong River basin and the Kum River have been analyzed, and have taken out the characteristics of each river basin. Also the runoff model have obtained for these basins.

신라대학교 주변 백양산의 동물상

노치권(외 6명) 신라대학교 자연과학연구소 2012 自然科學論文集 Vol.22 No.-

To study fauna of Silla University located in Mt. Baek-yang, we conducted six surveys from 29 May 2011 to 28 April 2012. We found five phyla 41 orders 197 species of animals inhabiting in this area: four phyla 27 orders 163 species of invertebrates and in vertebrates one order six species of fishes, three orders nine species of amphibians and reptiles, six orders 14 species of aves, and four orders five species of mammals. Of these species, some Korean endemic species were included: a fish of Rhodeus uyekii in a pond near main gate of this university, a frog of Rana coreana in a creek near a dormitory, and a rabbit of Lepus coreanus and a water deer of Hydropotes inermis in a grass region near a main stadium. A kind of hawk, Falco tinnunculus as a natural monument (animal) of no. 323-8, was observed on a building of medical and life science. A blue frog of Hyla japonica as a variation was found near a spring resort. Therefore, this area is considered an ecologically important area having a high animal diversity.

한국산야생등줄쥐(Apodemus agrarius coreae)의 해마복합체에서 neuropeptide Y 면역반응세포에 관한 연구

김무강,송치원,이경열,박일권,정영길,이강이,김종섭 충남대학교 수의과대학 동물의과학연구소 1997 動物醫科學硏究誌 Vol.5 No.-

Neuropeptide Y(NPY) was first isolated from porcine brain. This discovery has lead some workers to study the distribution of this peptide in the central nervous system of various mammals. In this study the distribution pattern of neuropeptide Y-immunoreactive neurons was examined in the hippocampal complex of Striped field mouse in Korea(Apodemus agrarius coreae) Experimental animals were anesthesized with thiopental sodium and perfused with 4% paraformaldehyde through the left ventricle and the ascending aorta. The brains were removed and transferred 10%, 20% and 30% sucrose solution. Sections were cut on a cryostat into 40㎛-thick. The tissue was immunostained with free-floating and avidin-biotm complex(ABC) method. The results obtained in this study were summarized as followings. 1. The most of NPY-IR neurons in hippocampus proper were found in the polymorphic layer and a few was found in the pyramidal layer. 2. The process run vertically in the deep polymorphic layer and run parallel to corpus callosum in superficial polymorphic layer. 3. The shapes of neurons in hippocampus proper were varied of polymorphic, oval or round, and the nerve fibers were found in the molecular laver and the hippocampal fissure. 4. The highest density in number of NPY-IR neurons on dentate gyrus were found in hilar area of dentate gyrus. 5. The most of NPY-IR nerve fibers were found in molecular cell layer.

일부 의료서비스업 VDT작업자의 상지 근골격계 증상의 정도와 관련된 요인

김형렬,원종욱,송재석,김현수,김치년,노재훈 大韓産業醫學會 2003 대한직업환경의학회지 Vol.15 No.2

목적: 본 연구는 상지 근골격계 질환의 유병율이 높은 집단으로 알려진 VDT작업자를 대상으로 이들의 근골격계 통증을 정량화하고, 정량화된 통증점수를 종속변수로 하여, 이와 관련성을 갖는 작업요인, 사회심리적 요인을 파악하고자 하였다. 방법: 서울지역에 위치한 1500병상 규모의 대학병원에 근무하고 단말기 작업을 주로 수행한다고 알려진 부서에 대해 병원내 안전관리과와 협의를 거쳐 163명을 연구대상으로 선정하였다. 이중 138명이 설문에 참가하였고, 이들을 최종 연구대상으로 하였다. 설문지를 가지고 연구변수를 측정하여, 나이, 근무연수, 가사노동의 정도 등의 인구학적 변수와 직무스트레스 및 사회적지지, 근골격계 증상, 통증의 양상 등을 조사하였다. 분석방법은 일반적 특성 및 작업특성, 통증의 양상을 성별에 따라 파악하였고, 통증점수의 타당성을 검정하기 위해, 통증점수의 구성요소와 통증점수를 시각적 상사척도와 상관성 검정을 시행하였다. 통증점수를 종속변수로 하고 직무요구도, 직무재량도, 동료의지지, 상사의지지, 여러작업환경특성, 인구학적 특징 등을 독립변수로 하여 다중회귀분석을 시행하였다. 결과: 첫째 통증의 정량화를 위해 사용한 통증평정지수는 시각적 상사척도와의 상관관계가 높은 것으로 나타나, 어느 정도의 타당성을 지닌 방법으로 해석할 수 있었다. 둘째, 이 통증 점수를 종속변수로 하여, 관련요인 분석한 다중회귀분석에서 직무요구도가 높을수록 통증의 정도가 높고(p<0.01), 여성일수록 남성에 비해 높은 통증점수를 보였다(p<0.01). 결론: 근골격계 질환의 높은 유병율을 보이는 집단의 경우, 이들의 증상 유무만을 가지고 관련요인을 분석하는 방법을 사용하기 어렵다. 따라서 이런 경우, 통증을 정량화하여, 다양한 관련요인을 찾으려는 노력이 필요하며, 본 연구에서 사용한 통증평정지수는 타당성을 지닌 정량화 방법이라고 여겨진다. 또한 본 연구에서는 직무요구도가 높을수록, 여성일수록 높은 통증점수를 보였다. Objectives: This study was designed to quantify symptoms in the upper extremities and to identify the pain related factors among hospital workers using video display terminals. Methods: The subject-group consisted of 138 employees working at a hospital using video display terminals. A structured questionnaire was used to estimate the participants' general characteristics, and job contents. Job stress was measured using Karaseks JCQ(Job Content Questionnaire). Pain in the upper extremities was identified according to the NIOSH symptoms survey criteria. Quantification was measured using Kim Chul's method. Pearson's Correlation analysis was used to identify validity in quantification. VAS(visual analog scale) was used to compare with this method. Multiple regression analyses elucidated the relationship between quantified pain and various work factors, such as job stress. Data were analysed with SAS 6.12 program. Results: First, in quantification using Kim Chul's method, the correlation between modified PRS(pain rating score) and VAS was extremely high by 0.60(P<0.01), indicating the high validity of PRS. Second, the groups of high job demand, high job control, and females were related to an increased pain level. Conclusions: These results suggest that the self pain assessment method, PRS, is valuable and useful in analyzing occupational musculoskeletal symptoms. High demand, job control and gender may influence the levels of pain. Prospective studies about musculoskeletal disorder are required.

절수에 의한 mongolian gerbil의 체중 변화에 관한 연구

이경열,송치원,김무강,조성환,류시윤,김상근,박배근 충남대학교 수의과대학 동물의과학연구소 1996 動物醫科學硏究誌 Vol.4 No.-

Recently, the mongolian gerbils have been used in neurology and the study of water metabolism because of their easy onset of epileptic seizure and typical resistance to water deprivation. The sebaceous glands which are under the control of the gonadal hrmones are differently developed from the other laboratory animals. However the physiological data on the changes of body weight following water deprivation in mongolian gerbils is not available until now. In order to investigate the effect of the water deprivation on the body weight, the animals were deprived of water for 15 days. The body weights were measured daily by the torsion balance. The mean, the standard deviation, the standard error and the coefficient of variance of the body weights were calculated. The correlation was examined. The potential regression equation was y = 61.5458 - 2.0533 * x + 0.0487 * x^2. The regression coefficient was 0.993. The results obtained were as follows : 1. The body weights of the mongolian gerbils were continuously decrased from the 1st day to the 15th day after the water deprivation. 2. The mean body weight on the water deprivation was 63.0±7.00g and that at the 15th day was 41.4 ± 5.42g. 3. The coefficients of the variance showed the highest (13.09) at the 15th day and the lowest (10.10) at the 10th day. the coefficients of the variance kept decreasing until the 10th day and thereafter gradually increased until the 15th day. 4. The changing rate of the body weight kept continuously decreasing from the 1st day to the 15th day showing the daily loss of 1.0 - 3.3%. 5.The deviations (M - QBW) between the measured values (m) and the theorical values (QBW) using the regression equation showed the highest (1.494) at the 1st day and the lowest (0.053) at the 13th day. 6. The sum of the devitions was 0.179 and that of the deviation square was 1.15. The sum of the deviation rates between the mesaured body weights and the theorical ones was 0.484. 7. The daily body weight loss showed the highest at the 1st day by 2.053g and the lowest at the 15th day by 0.591g. 8. The specific body weight changing rates which were the rate of the body weight changing velocity to the changed body weight were decreased from 0.033% at the 1st day to 0.014% at the 15th day.

급성 조증에서 기분안정제와 리스페리돈의 병합 투여 효과 : 다기관 개방연구

김광수,배치운,윤진상,김영훈,이양현,지익성,이철,송혜경,최성구,박원명 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.2

Ohjective : The primary purpose of this study was a replication of the effectiveness and tolerability of rispehdone in the treatment of patients with acute mania in very larger cohon in naturalistic treatment setting to extend the data on the effect and tolerability of risperidone in the treatment of patients with acute mania to Asian population. Methods : A total of 909 patients with DSM-TV criteria of bipolar disorder current manic and hypomanic episode, entered this large, open, multicentre study. The Young Mania Rating Scale (YMRS), Clinical Global Impression (CGI) and Simpson-Angus Rating Scale (SARS) were measured at baseline and weeks 1, 3 and 6, for the assessment of effectiveness and extra-pyramidal symptom (EPS). Results : This study showed statistically significant reduction of scores on the YMRS and CGI-s (mean change=-23.5±11.8, p<0.0001 ; mean change=-2.7±1.5, p<0.0001, respectively) from the baseline to the endpoint (week 6). Number of patients with 50% reduction or more in the YMRS and CGI-s scores was 693 (77.8%) and 630 (70.7%) at endpoint, respec-tively. There were no statistically significant increments of scores on SARS. Risperidone was generally well tolerated. Conclusion : The present larger open study demonstrates that hsperidone add-on therapy is effective and tolerable in treat-ment of bipolar disorder, replicating results in various controlled and uncontrolled studies from Western countries.

절수에 의한 Mongolian Gerbil 장기중량변화에 관한 분석연구

김무강,이기훈,이강이,송치원,이경열,권효정,박미선,정승혁,이행연,김명철 충남대학교 수의과대학 동물의과학연구소 2000 動物醫科學硏究誌 Vol.8 No.-

In this study, authors measured the each organ weight of the long term water deprived Mongolian gerbil, after then we calculated the mean, standard deviation, variance coefficient of the real measured organ weight and induced the organ weight change quantity, rate of quantity, deviation between measured and theological organ weight, deviation rate, sum of deviation rate, organ weight change rate, rate of organ weight by the mathematical formula. The results obtained as follow 1. The weight of the brains, lung, and testis were abruptly decreased after water deprived until the 5th day, after then slowly decreased until the 20th day. 2. The weight of the heart was decreased gradually from the first day until the 20th day. 3. The weight of the liver was abruptly decreased until the 10th day, after then the more slowly decreased until the 20th day. 4. The weight of the thymus, spleen, kidney, and pancreas were abruptly decreased until the 15th day, after then increased slowly until 20th day but the weight of the kidney was abruptly increased from the 10th day. 5. The organ weight change rate and the rate of the each day organ weight were very diversified.