계피 추출물의 항산화 효과 및 화장품소재의 응용

이영숙,유민정 한국피부과학연구원 2019 아시안뷰티화장품학술지 Vol.17 No.1

Purpose: This study examines potential abilities and commercial values of Cinnamon extract as bioactive and cosmetics ingredients. Methods: Cinnamomum cassia bark was extracted with hot water and 70% ethanol to examine its antioxidant effects through its total polyphenol, flavonoid content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, and (2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) ABTS radical scavenging activity. Also, this study examined its nitric oxide (NO) inhibition effect through RAW 264.7 cells into which inflammatory reaction was induced by lipopolysaccharides (LPS), and antihistamine activity in the RBL-2H3 cells. The antibacterial effects of Cinnamomum cassia bark extract on 8 types of bacteria were also studied. Results: As a result of measuring the antioxidant effect, the total polyphenol contents were 90.12 and 113.07 μg/mL, respectively, while the total flavonoid contents were 36.42 and 54.31 μg/mL, respectively. The DPPH scavenging effect was confirmed as 84.93% in the hot water extract and 90.25% in the ethanol extract for 400 μg/mL. The ABTS radical scavenging effect was 82.20% in the hot water extract and 92.21% in the ethanol extract for 400 μg/ mL. Upon measuring the NO inhibitive effect through the RAW 264.7 cells into which inflammation reaction was induced by LPS, the NO generation decreased to 14.57 μM in the hot water extract and 10.15 μM in the ethanol extract in the concentration of 100 μg/mL. This result, compared to the increase up to 20.11 μM by LPS, shows the NO inhibitive effect of Cinnamoum cassia bark extract. As a result of measuring the antihistamine activity in the RBL-2H3 cells, β-hexosaminidase increased to 176.21% by IgE-NDP whereas it was 117.25% in the hot water extract and 100.09% in the ethanol extract in the concentration of 100 μg/mL, confirming the inhibitive effect on β-hexosaminidase discharge. The antibacterial effect was confirmed on all bacteria through the measurement on the Cinnamomum cassia Bark extract on eight types of bacteria. Particularly, the antibacterial effect of the hot water extract and ethanol extract was found to be high in Staphylococcus epidermidis (S. epidermidis), Pityrosporum ovale (P. ovale). Conclusion: Cinnamomum cassia bark extract is deemed prospective as a natural functional cosmetics’ ingredient with excellent antioxidant, anti-inflammatory, antihistamine inhibitive and antibacterial effects. 목적: 본 연구에서는 계피 추출물의 생리활성 및 화장품 소재로서의 가능성과 산업적 활용가치를 확인하고자 하였다. 방법: 계피를 열수 그리고 70% 에탄올로 추출물하여 이용하여 총 폴리페놀, 플라보노이드 함량, 2,2-Diphenyl-1-picrylhydrazyl (DPPH) 소거활성, ABTS radical 소거활성을 조사하여 항산화 효과를 확인하였으며, lipopolysaccharides (LPS)로 염증반응이 유도된 RAW 264.7 세포를 통해 nitric oxide (NO) 생성 저해 효과와 RBL-2H3세포에서 항히스타민 활성을 알아보았다. 그리고 8종의 균에 대한 계피 추출물의 항균효과도 확인 하였다. 결과: 항산화 효과 측정 결과, 총 폴리페놀 함량은 각각 90.12, 113.07 μg/g 이고, 총 플라보노이드 함량은 36.42, 54.31 μg/g의 함량이 확인 되었다. DPPH 소거활성은 400 μg/mL에서 열수 추출물은 84.93%, 에탄올 추출물은 90.25%의 소거능이 확인 되었으며, ABTS 라디칼 소거활성을 측정 결과, 400 μg/mL에서 열수 추출물은 82.20%, 에탄올 추출물은 92.21%의 소거능이 확인 되었다. LPS로 염증반응이 유도된 RAW 264.7 세포를 통해 NO 저해 효과 측정 결과, LPS에 의해 NO의 생성량이 20.11 μM까지 증가된 것에 비해 100 μg/mL의 농도에서는 열수 추출물은 14.57 μM 에탄올 추출물은 10.15 μM 까지 감소된 것으로 보아 계피 추출물의 NO 생성 억제 효과를 확인할 수 있었다. RBL-2H3 세포에서 항히스타민 활성을 측정한 결과, IgE-DNP에 의해 β-hexosaminidased이 176.21%로 증가된 것에 비해 100 μg/mL의 농도에서는 열수 추출물은 117.25% 에탄올 추출물은 100.09%로 β-hexosaminidase 방출에 대한 억제 효과를 확인할 수 있었다. 8종 균에 대한 계피 추출물의 추출물의 항균효과를 측정한 결과 모든 균에서 항균효과가 확인 되었으며 특히 S. epidermidis, P. ovale 에서 열수 추출물과 에탄올 추출물 항균효과가 높게 확인되었다. 결론: 계피 추출물은 항산화 효과 및 항염 효과 및 항히스타민 억제효과 그리고 항균효과가 우수하였으 며, 천연 기능성 화장품 소재로서의 가능성이 있다고 판단된다.

Antioxidant and Proliferative Activities of Bupleuri Radix Extract Against Serum Deprivation in SH-SY5Y Cells

서미경,조혜연,이찬홍,구경아,박용기,이정구,이봉주,박성우,김영훈 대한신경정신의학회 2013 PSYCHIATRY INVESTIGATION Vol.10 No.1

Objective Bupleuri Radix (BR) is a major component of several Oriental herbal medicines used to treat stress and mental illness. There are evidences that antidepressant drugs modulate oxidative damage implicated in the pathophysiology of neuropsychiatric disorder, including depression. The aim of the present study was to investigate antioxidant and proliferative effects of BR against oxidative stress induced by serum deprivation in SH-SY5Y cells. Methods We examined the antioxidant effects of BR on a number of measures, including cell viability, formation of reactive oxygen species (ROS), superoxide dismutase (SOD) activity and levels of both Bcl-2 and Bax. We also investigated the effects of BR on cell proliferation using the bromodeoxyuridine (BrdU) assay, and used Western blot analysis to measure changes in expression of the cell cycle phase regulators. Results 1) Serum deprivation significantly induced the loss of cell viability, the formation of ROS, the reduction of SOD activity, down-regulation of Bcl-2 expression and up-regulation of Bax expression. However, BR extract reversed these effects in dose-dependent manner. 2) Serum deprivation significantly reduced cell proliferation. Western blot analysis revealed that serum deprivation significantly decreased cyclinD1 and phosphorylated retinoblastoma (pRb) expression, and increased p27 expression. On the other hand, BR dose dependently reversed these effects. Conclusion This study suggests that aqueous extract of BR may exert potent antioxidant effects and also play an important role in regulating cell cycle progression during neurogenesis. These effects of BR may be a potentially important mechanism of antidepressant underlying the observed antioxidant and proliferative effects.

SH-SY5Y 세포주에서 하고초, 금은화, 황금 에탄올 추출물의 6-OHDA로 유도된 산화적 손상에 미치는 영향

민관식,김수영,김민우,이기상,Min, Kwan-Sik,Kim, Su-Young,Kim, Min-Woo,Lee, Key-Sang 대한한방내과학회 2011 大韓韓方內科學會誌 Vol.32 No.4

Objectives : Categorized as 'cheongyeol' herbs, Herba Prunellae, Flos Lonicerae and Radix Scutellaria have been proven to have effect on degenerative brain disease, cerebrovascular disease and brain tumor because of their anti inflammation, antioxidant, or anticancer effects. In this study, we studied activity against reactive oxygen species and anti inflammation effect of these three 'Cheongyeol' herbs. Methods : We measured each herb's yield of ethanol extracts, phenolic contents and activities against DPPH, hydroxyl radical and superoxide anion. Also through 6-hydroxydopamine (6-OHDA) induced oxidative damage in SH-SY5Y human neuroblastoma cell line, we measured antioxidant effect and NO activity of the three herbs. From the three herbs, we chose Prunella Herba, which showed the highest antioxidant effect, and studied its cell survival rate and anti inflammation effect through COX-2 and iNOS. Results : All three herbs showed significant results, and especially Prunella Herba showed significant effect on phenol contents, antioxidant effect on various active oxygen and antioxidant, and anti inflammation effect through cell line. Conclusions : Further study of the origin concept of 'cheongyeol' and research into specific mechanisms and role in treatment of cranial nerve disease, seems warranted.

멜라닌생성 자동산화제인 FeSO4의 세포독성 및 멜라닌화에 대한 청미래덩굴 추출물의 영향

표애자,윤미영,양현옥 한국피부과학연구원 2013 대한피부미용학회지 Vol.11 No.1

To evaluate the cytotoxicity and protective effect of Smilax china L. (SC) extract on ferrous sulfate (FeSO4), an autooxidant of melanin formation, cell viability were analysed by XTT assay after human skin melanoma cells (SK-MEL-3) were cultured in media containing various concentrations of FeSO4. And also, the effect of antioxidant α-tocopherol on FeSO4-induced cytotoxicity was assessed. For the protective effect of SC extract on FeSO4-induced cytotoxicity, SK-MEL-3 cells were pretreated with 50 or 80 μg/㎖ of SC extract for 2 h before the treatment of FeSO4. And also, the antioxidative effects of SC extract were assessed by latate dehydrogenase (LDH) activity. In this study, FeSO4 remarkably decreased cell viability dose-dependent manner compared with control. And the XTT50 value was determined at 62.0 μM of FeSO4. In the effect of antioxidant, α-tocopherol effectively prevented FeSO4-induced cytotoxicity by the significant increase of cell viability. In the protective effect of SC extract on FeSO4-induced cytotoxicity, SC extract remarkably increased cell viability which was decreased by FeSO4-induced cytotoxicity, and also it showed the antioxidative effects such as a significant decrease of LDH activity. In the melanin generation, SC extract effectively blocked melanin generation by the decrease of tyrosinase activity and total amount of melanin. From these results, it is suggested that the cytotoxicity of FeSO4 was involved in oxidative stress, and also, SC extract effectively prevented the cytotoxicity and melanogenesis induced by an autooxidant of melanin formation, FeSO4 through antioxidative effect. Conclusively, SC extract may be a putative resources as an protective agent for oxidative stress-mediated skin hyperpigmentation via hyperactivity of autooxidant in melanin formation.

메꽃(Calystegia pubescens Lindl.) 추출물의 항균, 항산화 및항염증 효과에 관한 연구

변지아,신운교,장예진,황수빈,이선아,김가연,이진태,송일대,권용진 한국응용과학기술학회 2024 한국응용과학기술학회지 Vol.41 No.3

Calystegia pubescens Lindl. (C. pubesens)는 전통적으로 이뇨, 피로 및 혈당 강하 효과로사용되어 온 한국 토종 식물이다. C. pubesens는 이전 연구에서 항산화 및 미백 효과를 보였지만 항균및 항염증 특성에 대한 연구가 제한적이고 기능성 소재로서의 입증이 부족하다. 본 연구에서는 70% 에탄올로 추출한 C. pubesens의 잎(CPL)과 줄기(CPS) 추출물의 항균, 항산화 및 항염증 효과를 조사했다. CPL은 CPS보다 여드름균, 황색포도상구균, 대장균과 녹농균에 대해 우수한 항균 효과를 보였다. 또한CPL은 CPS보다 DPPH 및 ABTS+ 라디칼 소거 분석에서 더 높은 항산화 활성을 나타냈다. 따라서 CPL 은 CPS에 비해 우수한 항균 및 항산화 효과를 보였다. 이러한 결과를 바탕으로 RAW264.7 대식세포를이용한 CPL의 항염증 효과를 확인하기 위한 추가 연구를 진행하였다. 지질다당류(LPS) 자극에 의해 생성된 산화질소(NO)는 CPL에 의해 감소되었다. 또한, LPS에 의해 증가된 iNOS의 단백질 및 mRNA 발현은 CPL에 의해 감소되었으며, 이는 NO 생산과 동일한 경향을 나타내었다. 결론적으로 본 연구는CPL이 CPS에 비해 우수한 항균 및 항산화 효과를 가지고 있음을 확인하였으며, LPS에 의해 유도된iNOS 발현 및 NO 생성 억제를 통해 CPL의 항염증 효과를 입증했다. 본 연구 결과를 바탕으로 CPL의기능성 소재로서의 가능성을 제시한다 Calystegia pubescens Lindl. (C. pubesens) is a native Korean herb that has been traditionally used for its diuretic, fatigue, and blood sugar-lowering effects. In previous studies, C. pubesens has shown antioxidant and whitening effects, but research on its antibacterial and anti-inflammatory properties is limited, and its potential as functional materials is lacking. In this study, we investigated the antibacterial, antioxidant, and anti-inflammatory effects of leaf (CPL) and stem (CPS) extracts of C. pubesens extracted with 70% ethanol. When the anti-bacterial effect was confirmed, CPL showed superior anti-bacterial effect than CPS for C. acne, S. aureus, E. coli and P. aeruginosa. Additionally, CPL exhibited significantly higher antioxidant activity compared to CPS, as demonstrated by DPPH and ABTS+ radical scavenging assays. Therefore, CPL showed superior anti-bacterial and antioxidant effects compared to CPS. Based on these results, further investigation was conducted to confirm the anti-inflammatory effect of CPL using RAW264.7 macrophages. Nitric oxide (NO) produced by lipopolysaccharide (LPS) stimulation was significantly reduced by CPL treatment in a dose-dependent manner. Additionally, the protein and mRNA expression of iNOS was reduced by CPL in LPS-indueced RAW264.7 cells, which was consistent with NO production. In conclusion, this study confirmed that CPL has superior anti-bacterial and antioxidant effects compared to CPS, and demonstrated anti-inflammatory effect of CPL via inhibiting iNOS expression and NO production induced by LPS. Based on the result, we suggest the potential of CPL as a valuable functional materials.

Menadione에 의해 유발된 간독성에 미치는 홍삼사포닌의 영향

장봉준,배춘식,조용성,차용호,박창원,조대현,장경진,Jang, Bong-jun,Bae, Chun-sik,Cho, Yong-seong,Cha, Yong-ho,Park, Chang-won,Cho, Tae-hyun,Chang, Kyung-jin 대한수의학회 1997 大韓獸醫學會誌 Vol.37 No.3

It is known that 2-methyl-1,4-naphtoquinone(menadione, MD) induces hepatotoxicities both in vivo and in vitro. These toxic effects are believed to result from oxidative damages to hepatocytes by "active oxygen" species via one-electron reduction of the naphtoquinone. The ginsenoside(GS) is a complex mixture of individual ginsenosides which is known to produce a range of effects on the cardiovascular and central nervous systems. In particular, GS has an antioxidant effect. In this experiment we studied the effect of GS from red panax ginseng(red ginseng total saponin, RGTS) on free radical-induced liver injuries by MD. Administration of MD($150{\mu}M$) caused an increase in aspartate aminotransferase(AST) activities and lipid peroxidation, decrease in alkaline phosphatase(ALP) activities and total bilirubin levels in blood, caused depletion of GSH and changes of antioxidant enzyme(superoxide dismutase, catalase) activities are shown in liver tissue. Administration of RGTS restored the AST levels that increased by MD, but catalase showed no significant changes. RGTS also had an effect of restoring the GSH level and had some synergistic effects with SOD. These data suggest that RGTS may have some protective effects on liver injury which is related with the oxygen free radical.

차씨오일의 항산화 활성 및 뇌 해마유래 HT22 세포에서의 산화적 스트레스에 대한 보호효과

김종민(Jong Min Kim),박선경(Seon Kyeong Park),강진용(Jin Yong Kang),배성경(Seong-kyeong Bae),정가희(Ga-Hee Jeong),조경환(Kyoung Hwan Cho),김종철(Jong Cheol Kim),김재중(Jae Joong Kim),허호진(Ho Jin Heo ) 한국차학회 2018 한국차학회지 Vol.24 No.1

The antioxidant activities and hippocampal neuroprotective effects of tea (Camellia sinensis) seed oil against H 2 O 2 -induced oxidative stress were investigated to confirm its physiological effects. The antioxidant effects of tea seed oil were evaluated by measuring its 1,1-diphenyl-2-picrylhydrazyl, 2,2 -azino-bis (3-ethyl benzothiazoline-6-sulfonic acid) radical scavenging activities and malondialdehyde inhibitory effect. Tea seed oil was found to have significant antioxidant activity and inhibitory effect of lipid peroxidation. To confirm the ameliorating effect of the cholinergic system, the inhibitory effect of acetylcholinesterase (AChE) derived from PC12 was investigated. Tea seed oil was found to inhibit the AChE activity. Also, the protective effects of hippocampal neuronal cells against H 2 O 2 -induced neuronal cytotoxicity were investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide and 2 ,7 -dichlor-ofluorescein diacetate in HT22 cells. Tea seed oil was found to significantly increase the cell viability and reactive oxygen species scavenging activity.

어성초 메탄올 추출물로부터 항산화 효능을 가진 활성물질의 확인

김혜지(Hyeji Kim),황희성(Heesung Hwang),박수민(Sumin Park),강성욱(Sungwook Kang),김혜정(Hyejeong Kim),홍수경(Sugyeong Hong),김문무(Moon-Moo Kim),오영희(Yunghee Oh) 한국생명과학회 2017 생명과학회지 Vol.27 No.7

본 연구는 어성초의 메탄올 추출물을 이용하여 항산화 효능이 가장 좋은 fraction을 찾고, 항산화 효능을 나타내는 성분을 분석하였다. 메탄올 추출에 의한 유기 용매 별 분획에서 항산화 효과가 가장 좋은 ethyl acetate 분획물을 칼럼 크로마토그래피하여 12가지의 fraction 중 가장 높은 항산화 효과를 보인 Fr. 10을 이용하여 DPPH 라디칼의 소거활성, 환원력, 지질과산화, 세포독성, DNA 산화 및 DCFH-DA를 이용한 세포내 과산화수소 제거효과를 조사하였다. DPPH radical scavenging activity, reducing power, TBARS, cell viability, DNA oxidation and DCF fluorescence 12가지의 fraction들 중 항산화 효능이 가장 좋은 Fr. 10의 DPPH radical 소거 활성 결과로 64 μg/ml 농도에서 양성대조군에 근접하는 60%의 억제능을 보였다. Reducing power 결과로 32 μg/ml의 농도에서 140%로 양성대조군과 비슷한 결과 값을 보였다. TBARS 결과로 2 μg/ml에서 양성대조군과 같은 값의 활성산소 억제능이 나타난다. 또한 cell viability 결과로 32 μg/ml 이상의 농도에서 세포 독성에 의해 생존율이 감소하였고, DCF fluorescence 결과로 농도의존적으로 H2O2에 의한 산화적 손상을 억제하는 것으로 나타났다. DNA oxidation 실험결과 1 μg/ml 이상의 농도에서 DNA의 손상을 감소시킴을 확인할 수 있었다. IR 및 LC-MS를 이용하여 Fr. 10의 유효성분을 조사한 결과 rutin (분자량, 610)으로 확인 되었다. 결론적으로 어성초의 메탄올 추출물로부터 분리한 Fr. 10은 농도 의존적으로 항산화 효능이 우수하게 나타났고, 어성초는 화장품 및 기능성 식품의 소재로 활용될 수 있음을 시사한다. This study was carried out to evaluate the antioxidant effect of methanolic extract of Houttuynia cordata (HCME) and to identify a compound having antioxidant effect. The ethyl acetate fraction of HCME showed the highest antioxidant effect in organic solvent fractions. The fraction was then separated into 12 fractions by open column chromatography. Among these fractions, the fraction 10 (Fr. 10) with the highest antioxidant activity was isolated, and its antioxidant effect was evaluated by DPPH radical scavenging activity, reducing power, TBARS, cell viability, DNA oxidation and DCF fluorescence. The Fr. 10 at a 64 μg/ml showed 60% of inhibitory effect similar to that of vitamin C at 10 μg/ml, compared with blank group. The Fr. 10 at 64 μg/ml showed 264% of reducing power, compared with blank group. TBARS assay showed that the Fr. 10 at 64 μg/ml had 35.5% of inhibitory effect similar to that of vitamin E at 1,000 μg/ml, compared with blank group. The Fr. 10 above 32 μg/ml displayed cytotoxicity. However, it was observed that the Fr. 10, above 1 μg/ml reduced DNA damage. DCF fluorescence assay showed that the Fr. 10 inhibited oxidative stress by H₂O₂ in a dose dependent manner. The compound of Fr. 10 was identified to be rutin whose molecular weight is 610 by the IR and LC-MS analyses. Therefore, these results suggest that the rutin of Fr. 10 could use as a natural antioxidant for development of cosmetics and functional foods.

Effect of Antioxidant and Antifungal Activity of Deacetylated Hyaluronic Acid

( Choon Geun Lee ),( Jae Kweon Park ) 한국키틴키토산학회 2015 한국키틴키토산학회지 Vol.20 No.3

HA is unbranched linear polysaccharide chain which has a high molecular weight composed of D-glucuronic acid (GlcUA) and N-acetyl-D-glucosamine (GlcNAc). It was distributed broadly throughout connective tissue, joints, epitherial in human and mammals. In this study, deacetylated hyaluronic acid (DA-HA) and high molecular weight hyaluronic acid (HMWHA) were studied to examine the antioxidant activity and antifungal effect. DA-HA indicated significant antioxidant relative activity of ferric ion reducing antioxidant power (FRAP) and 2,2``-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) di-ammonium salt (ABTS) radical cation scavenging, respectively. In particular, DA-HA showed higher antioxidant activity than HMWHA. Also, It has significant antifungal effect on P. italicum known as fungi of clementine peel. These results demonstrated that DA-HA could be considered as a material of functional cosmetics owing to superior antioxidant activity and applied to agricultural industry as a fungi static agent.

Cisplatin의 세포독성에 대한 레몬밤 추출물의 억제 효과

최유선 ( Yu Sun Choi ),김선주 ( Sun Ju Kim ),유영월 ( Yeong Wol Yu ),임요섭 ( Yo Sup Rim ),황은희 ( Eun Hee Hwang ),이화정 ( Hwa Jeong Lee ),최은영 ( Eun Young Choi ),장병수 ( Byung Soo Chang ),정인주 ( In Ju Jung ) 대한미용학회 2013 대한미용학회지 Vol.9 No.1

The purpose of this study is to evaluate the protective effect of Lemon balm (Mellisa officinalis) extract on the cytotoxicity induced by cisplatin, pharmaceutic alopecia inducer. To achieve this, cisplatin-induced cytotoxicity was assessed by XTT assay after human skin fibroblasts (Detroit 551) were cultured in media containing various concentrations of cisplatin. And also, the effect of vitamin E was examined on the cisplatin-induced cytotoxicity. For the protective effect of Lemon balm extract on cisplatin-induced cytotoxicity, Detroit 551 cells were pretreated with 80 or 100 μg/mL of Lemon balm extract for 2 hours, and also, antioxidative effects of Lemon balm extract such as 1,1-diphenyl-2-picrylhydrazyl (DPPH)-radical scavenging activity and lactate dehydrogenase (LDH) activity were analysed. The results were as follows. Cisplatin showed a significant decrease of cell viability in dose dependently, and the XTT50 value was calculated at 25.7 μM. In the effect of vitamin E, it significantly increased cell viability which were decreased by the cisplatin-induced cytotoxicity. In the protective effect of Lemon balm extract on cisplatin-induced cytotoxicity, it significantly increased cell viability which was decreased by cisplatin-induced cytotoxicity, and also it showed the DPPH-radical scavenging activity and LDH inhibitory activity. Therefore, these results suggested that the cytotoxicity of cisplatin may be involved in oxidative stress, and also, Lemon balm extract effectively prevented the cytotoxicity induced by cisplatin via antioxidative effect. Conclusively, the natural extract like Lemon balm may be useful for the development as antioxidative agent via the prevention of the cytotoxicity induced by pharmaceutic alopesia inducer correlated with oxidative stress.