$1{\alpha},\;25\;Dihydroxyvitamin\;D_3$이 랫드 골다공증에 미치는 영향

배춘식,최석화,Bae, Chun-Sik,Choi, Seok-Hwa 한국현미경학회 2002 Applied microscopy Vol.32 No.3

Sprague-Dawley rat에 complete Freund's adjuvant (CFA)를 투여하여 유발한 골다공증에 미치는 $1{\alpha}$, 25 dihydroxyvitamin $D_3\;(Vit\;D_3)$의 효과를 알아보기 위하여 실험동물을 정상 대조군, CFA 투여 대조군, CFA 투여후 $1{\alpha}$, 25 dihydroxyvitamin $D_3\;0.01{\mu}g/kg$ 투여군 (Vit $D_{3}L$) 및 $0.1{\mu}g/kg$ 투여군(Vit $D_{3}H$)으로 분류하여 경구로 3주간 투여하였다. 골다공증의 억제 효과를 알아보기 위하여 골밀도와 골함유량의 변화 및 주사전자현미경적 소견을 관찰한 결과 다음과 같은 결론을 얻었다. 골밀도와 골함유량은 $1{\alpha}$, 25 dihydroxyvitamin $D_3$ 투여용량과 부위에 따라 차이가 있었는데 Vit $D_3$ 투여군이 CFA 투여 대조군과 비교하여 골밀도와 골함유량이 증가하였으며 Vit $D_{3}H$군이 Vit $D_{3}L$군보다 좀 더 효과적이었다. 대퇴골의 주사전자현미경적 소견은 Vit $D_3$ 투여군의 골피질과 골소주의 손상이 CFA 투여 대조군보다 억제되었음을 볼 수 있었으며 Vit $D_{3}H$ 투여군이 Vit $D_{3}L$ 투여군보다 좀 더 효과적이었다. 이상의 결과를 종합해 보면 $1{\alpha}$, 25 dihydroxyvitamin $D_3$의 투여는 CFA에 의해 유발된 rat의 골다공증 모델에서 골다공증의 진행을 억제하였고 Vit $D_{3}H$ 투여군이 Vit $D_{3}L$ 투여군보다 좀 더 효과적이었다.

손상된 흰쥐의 좌골신경에 저출력 레이저 조사후 전기생리학적 변화

배춘식,신수범,김권영,Bae Chun-Sik,Shin Soo-Beom,Kim Kweon-Young 한국생명과학회 2006 생명과학회지 Vol.16 No.1

The purpose of this study was to determine effects of the Ga-As (Gallium-Arsenide) Dens-Bio laser on mechanically injured sciatic nerves of rats. The improvement of the injured rat sciatic nerve was evaluated by measuring of nerve conduction velocity and amplitude of compound muscle action potential. The sciatic nerves of forty male Sprague-Dawley rats were compressed with hemostatic forceps for 30 seconds. The experimental group was divided into 4 subgroups according to the duration of treatment. Lower power infrared laser irradiation was done transcutaneously to the injured sciatic nerve area, 3 minutes daily to each of four treatment groups for 1, 3, 5, and 7 weeks, respectively. Compound muscle action potential and nerve conduction velocity of sciatic nerve were obtained before nerve injury and at 1, 3, 5, and 7 weeks after injury. There were significant difference of the nerve conduction velocity and amplitudes of compound muscle action potential between the treatment group and non-treatment group at 1, 3, and 5 weeks after laser treatment. However, there were no differences found between the electrophysiologic parameters that were measured after 7 weeks in two groups. There was significant correlation between the increment of compound muscle action potential and nerve conduction velocity after time course according to laser treatment. In conclusion, the low power laser treatment had improved the sciatic nerve function, and therefore these results may provide the basic data to clarify the neurological recovery and treatment after incomplete peripheral nerve injury.

손상된 좌골신경의 재생에 미치는 Ga-As 적외선 레이저의 효과

배춘식,임성철,박석천 한국임상수의학회 2002 한국임상수의학회지 Vol.19 No.3

The purpose of this study was to examine the effect of Ga-As(Gallium-Arsenide, wave length; 904nm) infrared laser irradiation on healing of the experimentally crush injured rat sciatic nerves. The bilateral sciatic nerves of 43 adult male Sprague-Dawley rats were compressed surgically with a straight hemostat (1mm width). The right legs of all the rats were irradiated using a 27mW Ga-As infrared laser (laser irradiated group). The radiation procedure was administered for 3 minutes every day for 1, 3, 5, and 7 weeks in each group. Left legs were not irradiated and served as the control group. The numbers of total myelinated axon and degenerated myelin in the sciatic nerves of bilateral legs were measured and analyzed with image analysis system in order to make a morphological analysis of the effect of the Ga-As infrared laser on injured nerves. Total number of myelinated axons was increased with time interval, especially in the 1, 3, and 5 week of irradiated group. Conversely, the number of degenerated myelin was decreased with time interval, especially in the irradiated group. The effects in the irradiated group were more pronounced than those of the control group. In conclusion, the Ga-As infrared laser irradiation is a useful adjuvant therapy to the regeneration of the peripheral nerve injury.

홍화 (Carthamus tinctorius L.) 씨 분말의 골다공증 치료효과

배춘식,박창현,조형진,한혜정,강성수,최석화,엄창섭 한국현미경학회 2002 Applied microscopy Vol.32 No.3

The therapeutic effect of safflower seed powder on experimental osteoporosis in the rat induced by ovariectomy was evaluated. Thirty Sprague-Dawley rats were ovariectomized at the age of 12 weeks. Seven weeks postovariectomy, rats were divided into two groups: control and safflower seed powder treated group. Five animals from each group were sacrificed at the following time points: 1, 3, and 5 weeks. Scanning electron microscopic observation and morphometric analysis of the tibiae epiphysis showed that the administration of safflower seed powder significantly prevented reduction of cortical bone width and bone volume compared with the control group. In conclusion, safflower seed powder contains something that prevent bone loss due to estrogen deficiency, and was effective in preventing the osteoporotic decrease of bone mass. 홍화씨는 한방 및 민간에서 뼈에 우수한 작용이 있는 것으로 알려져 오랫동안 복용되어 왔다. 최근 고령화 사회를 맞이하면서 골다공증 문제는 사회적인 문제로 인식되고 있으며 저자들이 기존에 실시한 실험결과에 의하면 홍화씨 분말은 난소를 적출한 rat에서 골다공증의 예방효과를 나타내었다. 그러나 아직까지 골다공증이 유발된 rat에서 홍화씨 분말이 미치는 효과에 관한 실험적 자료가 제시된 것은 별로 없다. 이에 저자들은 홍화씨 분말이 골다공증의 치료에 미치는 효과를 알아보기 위하여 본 실험을 실시하였다.실험동물은 체중 230g의 12주령의 Sprague-Dawley Rats를 사용하였으며, 양쪽 난소제거 후 7주부터 홍화씨 분말을 매일 0.3g 씩 복용시키면서 1, 3 및 5주 후에 경골을 채취하여 관찰하였다. 채취된 조직은 통상적인 주사전자현미경 시료제작법으로 고겅과정을 거친 후 10% 질산으로 12시간 탈회하여 뼈의 단면을 노출시키고 탈수, 건조 및 금도금 과정을 거쳐 주사전자현미경(Hitachi, S-450)으로 관찰하여 촬영하였다.관찰결과 대조군은 골수강에서 피질골까지의 두께의 감소와 골수강의 확장이 심하였으나, 홍화씨 분말을 투여한 실험군에서는 1주에서 5주까지 거의 같은 소견을 나타내었다.이상의 결과를 종합해보면 홍화씨 분말은 여성호르몬 결핍으로 인한 골다공증의 치료에 효과가 있는 것으로 사료된다.

Extracts from Erythronium japonicum and Corylopsis coreana Uyeki reduce 1,3-dichloro-2-propanol-mediated oxidative stress in human hepatic cells

배춘식,윤철호,안태호 한국식품과학회 2019 Food Science and Biotechnology Vol.28 No.1

In this study, it was demonstrated that 1,3-dichloro-2-propanol (1,3-DCP) induced oxidative stressand cell death in HuH7, human hepatocytes. The protectiveeffects of Erythronium japonicum (E. japonicum) andCorylopsis coreana Uyeki (C. coreana Uyeki) extractsagainst 1,3-DCP-treated cells were also investigated. First,the activities of superoxide dismutase (SOD) and catalase(CAT) were diminished by the treatment of 1,3-DCP. Moreover, 1,3-DCP stimulated the expression and catalyticactivity of cytochrome P450 2E1 (CYP2E1), an enzymethat generates reactive oxygen species in the liver. Incontrast, co-treatment of 1,3-DCP with the extracts significantlydecreased ROS generation and inhibitedCYP2E1 activity without affecting its expression. The coadministrationof extracts also restored the activities ofSOD and CAT reduced by 1,3-DCP and protected against1,3-DCP-mediated cell death. In conclusion, these resultssuggest that 1,3-DCP induces oxidative stress through theelevated CYP2E1 level, which is inhibited by the extracts,protecting cells against the effects of 1,3-DCP.

Therapeutic Effects of Safflower (Carthamus tinctorius L.) Seed Powder on Osteoporosis

배춘식,박창현,조형진,한혜정,강성수,최석화,엄창섭,Bae, Chun-Sik,Park, Chang-Hyun,Cho, Hyung-Jin,Han, Hye-Jeong,Kang, Seong-Soo,Choi, Seok-Hwa,Uhm, Chang-Sub Korean Society of Microscopy 2002 Applied microscopy Vol.32 No.3

The therapeutic effect of safflower seed powder on experimental osteoporosis in the rat induced by ovariectomy was evaluated. Thirty Sprague-Dawley rats were ovariectomized at the age of 12 weeks. Seven weeks postovariectomy, rats were divided into two groups: control and safflower seed powder treated group. Five animals from each group were sacrificed at the following time points: 1, 3, and 5 weeks. Scanning electron microscopic observation and morphometric analysis of the tibiae epiphysis showed that the administration of safflower seed powder significantly prevented reduction of cortical bone width and bone volume compared with the control group. In conclusion, safflower seed powder contains something that prevent bone loss due to estrogen deficiency, and was effective in preventing the osteoporotic decrease of bone mass.

난소적출 흰쥐에서 홍화(Carthamus tinctorius L.)씨 분말이 골흡수에 미치는 영향

배춘식,박창현,장병준,김휘율,조익현,엄창섭,Bae, Chun-Sik,Park, Chang-Hyun,Chang, Byung-Joon,Kim, Hwi-Yool,Cho, Ick-Hyun,Uhm, Chang-Sub 한국현미경학회 2001 Applied microscopy Vol.31 No.2

Safflower has been cultivated in Korea and thought to have excellent effects on bone in oriental medicine and folk remedy and has been taken for a long time. Safflower is thought to be helpful for the development and sustenance of bones according to the result of recent assay of its components. Otherwise, any reliable experimental data have not been suggested so far. We have carried out this study to examine the prophylactic effects of safflower-seed-powder on the prevention of osteoporosis induced by the ovariectomy 12 Weeks-old Sprague-Dawley rats weighing about 230 g was kept in the experimental condition and used in this study. Animals were taken 0.3 g of safflower-seed-powder once a day for 1, 3, 5, and 7 weeks after ovariectomizing both ovaries and observed the fine structure of tibia. Tissues were fixed with traditional SEM preparation methods and decalcified for 10 hours with 10% nitric acid and dehydration, drying, and gold-coating were followed by the routine procedures and observed with scanning electron microscope (Hitachi, S-450). Loss of bone was started just after ovariectomy and thickness of bone from the medullary cavity to the compact bone was reduced and the extension of medullary cavity was serious in the control group of 7 weeks. Experimental groups taken safflower-seed-powder showed similar findings from 1 week to 7 weeks. These results suggest that the safflower-seed-powder is thought to be efficient for the prevention of osteoporosis owing to the deficiency of female sex hormone.

랫드에서 1α,25 dihydroxycholecalciferol의 골다공증성 골절 치유효과

배춘식,Bae, Chun-sik 대한수의학회 1999 대한수의학회지 Vol.39 No.5

Osteoporosis is defined as a decrease in bone mass that leads to an increased risk of fracture. The therapeutic effect of $1{\alpha}$,25 dihydroxycholecalciferol, the hormonal form of vitamin $D_3$ that mediates calcium translation in intestine and bone, on the healing process of fracture has still been controversial. These studies were designed to understand the healing process of normal fibular fracture, the osteoporotic changes after ovariectomy, and the therapeutic effects of $1{\alpha}$,25 dihydroxycholecalciferol on the osteoporotic fracture in rats. The simple transverse fractures of rat fibulae were produced with a rotating diamond saw. The changes of the biochemical and mechanical indices of rats were investigated. The mechanical study based on bending test revealed the healing of the fibular fracture in the 5th week after simple transverse fracture. The osteoporosis impaired more the healing of osteoporotic fibular fracture than normal non-osteoporotic fibular fracture. The healing process of osteoporotic fracture was facilitated by the treatment with $1{\alpha}$,25 dihydroxycholecalciferol, however, was delayed more than the healing process of normal fracture. The bone strength based on the bending test also confirmed this tendency. The bone strengths in the 5th week after fracture of normal bone, osteoporotic bone, and $1{\alpha}$,25 dihydroxycholecalciferol-treated osteoporotic bone were 75%, 41%, and 67%, respectively, in comparison with those of intact bone. In conclusion, $1{\alpha}$,25 dihydroxycholecalciferol was effective in promoting the osteoporotic fracture healing.

국내 실험동물 생산 및 공급과 관련된 사용자 요구사항 분석

배춘식 한국실험동물학회 2019 한국실험동물학회 학술발표대회 논문집 Vol.2019 No.1

난소적출 랫트 비골의 시간경과에 따른 골다공증성 변화

배춘식,박창현,엄창섭,Bae, Chun-Sik,Park, Chang-Hyun,Uhm, Chang-Sub 한국현미경학회 1999 Applied microscopy Vol.29 No.3

Osteoporosis means a deficiency in the amount of bone tissue in the skeleton or part of the skeleton. Osteoporosis is a lesion, not a specific disease. 'Osteoporotic' describes the slate of a bone or skeleton at a given time. Osteoporosis may be diagnosed subjectively by visual appraisal, or objectively by measurement of radiographs, sawn bones, or microscopic sections. This study was carried out to make clear of the influence of ovariectomy on time course of fibular osteoporosis in rats. Seven weeks after ovariectomy, osteoporosis was evident, when the size of the bone marrow cavities significantly increased and the width from the bone marrow cavity and cortex significantly decreased than normal.