Streptozotocin 유도 당뇨쥐에서의 Phospholipase A₂, Cyclooxygenase 활성과 Thromboxane 및 Prostacyclin합성

양정아,김성옥,최정화,곽오계,이순재,장현욱 영남대학교 약품개발연구소 1998 영남대학교 약품개발연구소 연구업적집 Vol.8 No.-

당뇨병에서의 혈전생성 기전에 관련된 주된 요인을 관찰코져 흰쥐를 4주간 사육한 후 streptozotocin(STZ)으로 당뇨를 유발한 후 6일째에 희생하여 당뇨쥐에 있어서의 phospholipase A₂ 활성변화에 따른 조직의 과산화적 손상과 혈소판 응집능 등의 변화를 관찰한 결과는 다음과 같다. 체중증가는 STZ를 투여한 후에는 STZ 투여군에서 현저하게 감소하였다. 식이섭취에 있어 STZ injection 후, DM군은 16% 증가하였다. 식이효율은 STZ injection 후 DM군은 정상군에 비해 유의적으로 감소하였다. 혈청 중의 총 지질과산화물가는 정상군에 비해 DM군은 2.5배 높았다. HDL 중의 TBARS는 정상군에 비해 DM군은 약 1.6배 정도 높았고 LDL 중의 TBARS는 정상군에 비해 DM군에서 4.8배의 높은 증가를 보였다. 혈소판 phospholipase A₂ 활성은 DM군은 정상군에 비해 42% 증가하였다. 혈소판 cyclooxygenase 활성은 정상군에 비해 DM군이 2배 정도 높은 수준이었다. 혈소판 중의 TXA₂ 농도는 정상군과 비교하여 DM군에서 169%로 현저하게 높았고 대동백 PGI₂의 농도는 정상군에 비해 DM군은 낮은 수준을 보였으며 대동맥 PGI₂와 혈소판 TXA₂의 비는 DM군에서 정상군에 비해 55% 낮았다. 결론적으로 STZ 유발 당뇨쥐에 있어서는 혈청 지질과산화물 수준의 증가와 더불어 phospholipase A₂ 활성이 증가되었으며 따라서 cyclooxygenase가 유도하는 AA cascade 활성화에 의해 TXA₂ 생성증가 및 PGI₂/TXA₂ ratio의 감소가 관찰되었다. 이러한 결과는 당뇨병 질환에서 나타나는 동맥경화증, 심혈관계 질환 등의 여러 혈관계의 병리적 현상들이 당뇨 상태에서의 지질대사 이상으로 인한 지질과산화물의 증가와 그의 독성으로 인한 여러 혈소판 관련 인자들의 활성화와 밀접하게 연관되어 나타나는 것을 시사하는 것이라 하겠다. The relation between lipid peroxidation and thrombotic reaction were investigated in streptozotocin (STZ)-induced diabetic rats. Sprague-Dawley male rats weighing 100±10gm were randomly assigned to normal and STZ-induced diabetic group(DM). Diabetes was experimentally induced by intravenous injection of 55mg/kg of body weight of STZ in citrate buffer(pH 4.3) after 4 weeks feeding of basal diet. Animals were sacrificed at the 6th day of diabetic states. Body weight gains were lower in diabetic group after STZ injection. Serum levels of thiobarbituric acid reacting substances(TBARS) that were markedly increased in DM group compared with of normal group. TBARS levles of HDL and LDL were similar patterns to total TBARS of serum. Activities of platelet phospholipase A₂(PLA₂) were higher in diabetic group than those of normal group. Activities of platelet cyclooxygenase were 106% in DM group than normal group. Platelet thromboxane A₂(TXA₂) formation was increased in DM group than normal group. Production of aortic prostacyclin(PGI₂) was lower in diabetic group than that of normal group. PGI₂/TXA₂ ratios were decreased by 55% in DM groups than those of normal group. The present results indicate that STZ-induced diabetic rats are more sensitive to oxidative stess which leads to acceleration of lipid peroxidation and platelet aggregability. In conclusion, accelerating effect of lipid peroxidation and thrombogenesis in diabetic state is regarded to be resulted from enhancement of PLA₂ activity and arachidonic acid metabolism, inhibition of antiaggregating agent and aortic PGI₂ formation.

秋史 金正喜의 濟州流配文學 硏究

梁淳珌,金奉玉 제주대학교 1991 논문집 Vol.32 No.1

The purpose of this study is to understand Choosa's literature by analyzing 20 exile chinese poems and 48 exile letters(27 Chinese letters and 21 Korean letters), which was written for nine years of his exille. Conclusions of this study are as follows: (Ⅰ) Exile chinese poetry expresses emotion in the exile's life, such as affection for the kings, repent of individual situation, life in seclusion, love for nature, nostalgic reminiscence, great sarrow, lament for the world, tast, and transience. This proves that while living in seclusion, Choosa not only eulogized the nature but also devoted himself to an artistic life with the inspiration obtained from probing nature. (Ⅱ) In analyzing exile letters, I quoted Busemann's emotional and intentional phase replacement theory and through this method, I concluded the followig. 1) His fraternity with his brother and elder cousin when he was exiled shows emotional phase. This derived its origin from environmental factors of an isolated island and from his inner state of mind covered with hopelessness and solitude. 2) His emotional phase for his wife when he was exiled to Cheju-do, was his confirmed love and respect, which we can't find in a traditional and noble family. But I found out this in his letters sent to his wife. He wrote the letters to his wife using honorific expressions and unique words. I could reconfirm this in a mourning poem and a funeral address expressing his regret over his wife's death. Especially, more attention should be paid to use creative words for his wife. 3) His emotional phase for his children twmed out to be a stern father. He said that mountains and streams couldn't break affecti on between them son, even though they lived at a long distance each other. I could find his emotional phase in taking attitude toward his son's reading. And he has an intellectual and conservative phase, from the viewpoint of advising his adopted son, Sang-moo to observe his family precepts "To behave oneself decently". 4) He regarded sacrificial rites as a sincerity, and he was glad to take a descendant. In this sence, we can claim that his intention to be anxious about his family shows us his distinct learning toward conservatism. 5) He struggled not to lose his moral sense of a classical scholar during his exiled life to Cheju-do. He devoted all his energies to all things. He always carried about books with him, and his action was sincere. He completed "CHOOSA-CHE; his unique style of penmanship" and "SAEHAN-DO; his painting showing one's true value when he is in adversity", while in Cheju-do. In view of this, we contend that he was great scholar who acted up to his principles and that he overcome difficulties, frustrations, and despairs during his exiled life. 6) He realized his rational intention, by maintaining the relation of teacher and pupil. Only if the pupils devoted themselves to their studies, he taught them with ail his might. Surely he was looked up to as their true preceptor. Letters are materials showing personal aspects such as ways of thinking and ways of life. From this point of view, this study aims to understand Choosa's letters written when he was in exile. For this purpose, I made an concrete analysis of the letters based on the phase-replacement theory. It is highly valued that Choosa wrote 21 letters to his wife and daughter-in-law, which reflects his real life-style and practical conscionsness of his languge and literaure as a means of communication.

老人女性의 운동에 대한 認識 및 與件과 實態에 관한 調査硏究

梁順子,權永玉,元周淵 忠南大學校體育科學硏究所 1988 體育科學硏究誌 Vol.6 No.1

The purpose of this study was to provide a fundamental information for plan for positive and active participation or execution of exercise in Female elderly. Questionaire about the Leisure activities, the understanding for exercise and health, the actual and given condition of exercise participation and facilities was used for this study. 330 Aged women (over 60 year-old) who live in Seoul city were answered the Questionaire. Following conclusions were obtained were obtained : 1. Aged women's main daily works are managing house hold duties, attending a lecture in College for elderly, Seeing and Hearing of T. V. or Radio. And their Leisure activities are very simple (not various) and Static, for example seeing and Hearing of T. V. or Radio, gardening, reading, chess and Korean playing cards etc. 2. Aged women are very interested in Health and they point out illness and lack of exercise as a factor of weakness. Furthermore they understand the necessity of exercise for health and their daily life in old age, and also have a lot of intention to do exercise. 3. Exercises that aged women are enjoying or want to enjoy are taking a walk, mountain climbing, physical exercises, Aerobic dance, Badminton, and Jogging. 4. Almost aged women are doing exercise sometimes or usually, and also are seeing and hearing a T. V. or Radio for sports game. 5. Places that aged women are doing excercise are generally not well equipped place (e. g. medicinal water fountain, low hill, and around their house). Besides almost doing exercise alone or just with intimate friends and family. Club sports activities are not general. 6. As inconvinient factor for exercise, a lot of aged women pointed out the not specially equipped or established sports facilities for elderly, lack of number of equipment, and the long distance from home to facilities.

갑상선 질환에서 혈청 Microsome 항체 측정의 의의

윤영옥,장순모,박호자,이준열,양기화,정인숙,강석진 대한임상병리사협회 1984 임상병리사회지 Vol.16 No.1

STZ 유발 당뇨쥐에서 시간에 따른 생리적 항산화계의 변화

이순재,양정아,김성옥,최정화,신주영,채영미,차복경 대구효성가톨릭대학교 응용과학연구소 1997 응용과학연구논문집 Vol.5 No.-

The purpose of this study was to investigate change of physiological antioxidative system according to the time in streptozotocin-induced diabetic rats. Sprague-Dawley male rats weighing 150±10gm were randomly assigned to one normal and five STZ-induced diabetic groups. Diabetic groups were classified to experimental period. Diabetes was experimentally induced by intravenous injection of 55 mg/kg of body weight of STZ in citrate buffer(PH 4.3) after 6 weeks feeding of three experimental diets. Animals were sacrificed at 0, 3, 6, 12, 18th days of diabetic states. 1. Activity of superoxide dismutase(SOD) in liver was increased in that of diabetic mellitus(DM) groups at 3th day but that of DM groups was not significant from 6th day according to period. Glutathion peroxidase(GPX) was significantly decreased in DM groups from 6th day 2. Reduced glutathione(GSH) contents in liver significantly decreased in diabetic groups from 3rd day compared with those of DM groups according to the period. Oxidized glutathione(GSSG) was higher from 6th day. GSH/GSSG ratio was significantly lower than that of normal group from 3rd day to all experimental period 3. Contents of vitamin E in liver of DM groups were significantly decreased compared with that of normal group from 6th day. 4. Lipid peroxide(LPO) contents in liver of DM groups were significantly increased compared with that of normal group from 3rd day. The present results indicate that STZ-induced diabetic rats were reduced by antioxidative defense system and taken by peroxidate damage in tissue compared with normal group from 3rd day or 6rd day after injection STZ. It lended to acceleration all diabetic groups but no significance according to the experimental time.

엘라스타제의 토끼에 대한 최기형시험

안병옥,백남기,이순복,김원배,양중익,이영순,임윤규 A.R. Liss 1990 ENVIRONMENTAL MUTAGENS AND CARCINOGENS Vol.10 No.2

돼지 췌장 유래 엘라스타제의 항원성 시험

김순회,백남기,안병옥,이상득,이순복,김원배,양중익 A.R. Liss 1990 ENVIRONMENTAL MUTAGENS AND CARCINOGENS Vol.10 No.2

자기 효능의 개념 분석

서순림,이은남,박송자,양영희,이동숙,최은옥,구미옥,김인자,이인숙,김성재,박영임,이은옥 서울대학교 간호대학 간호과학연구소 1997 간호학 논문집 Vol.11 No.1

Today human needs for health promotion and maintenance are increasing. The nursing progression has a great responsibility for helping clients to practice good health behavior. self efficacy was found to be potent predictor in initiating and maintaining the health-related behavior. Therefore, the concept is important in nursig intervention for change of health behavior. The purpose of this paper was to analyze and clarify the meaning of the concept, self efficacy. This study used Walder & Avant's process of concept analysis. Critical attributes of self efficacy were : 1) positive perception of his won capability; 2) intrapersonal strength; 3) possibility of being learned; 4) individuality; 5) specificity; 6) generalizability. Antecedents of self efficacy consist of those facts that 1) new situation occurs; 2) individuals have needs to change the new situation; 3) there are some required actions in new situation. Consequences occurring as a result of self efficacy consist of those facts that 1) individual initiates the of failure; 5) cope with the situation; 6) controls the situation; 7) the level of self efficacy is enhanced; 8) the level of self-esteem is enhanced.

ICNP를 적용한 학교간호현상 및 특성과 초·중·고등학교의 학교간호현상 비교

김영임,왕명자,양순옥,현혜진,박은옥 韓國學校保健學會 2004 韓國學校保健學會誌 Vol.17 No.1

Purpose : this study purposed to investigate school nursing phenomena in Korea by applying ICNP, the international standard nursing classification system developed by ICN, and to compare school nursing phenomena at elementary, middle and high schools. Method : The subjects of this study are 110 nursing teachers from 82 elementary schools, 16 middle schools, and 12 high schools. In the survey, subjects were asked to score the degree to which phenomena and characteristics of Koran school nursing, identified in previous research, on a 5 point scale. Questionnaires were distributed and recovered by mad and email. The period of data collection was 6 months from July to December 2003. The general characteristics of schools and nursing teachers were represented with frequencies and percentages, the phenomena and characteristics of school nursing with the score of the questions, and the phenomena school nursing by school grade with ANOVA and Duncan's posterior analysis. Results : 1) As for the characteristics of schools according to school nursing phenomena related to human behavior, the mean score of questions on inadequate stress management was highest at 3.24 points followed by the score on inadequate weight control (3.23), inadequate eating habits (3.22), the risk of spine disorders (2.68), inadequate emergency management (2.62), inadequate response to sex -related problems (2.19), and smoking and drug use (1.85). 2) As for the characteristics of schools according to school nursing phenomena related to human function, the mean score of questions on oral health management was highest at 3.11 points followed by the score on the risk of digestive system disorder (2.87), improper eyesight management (2.81), the risk of respiratory system disorders (2.75), lack of sexual identity (2.52), and inadequate contagious disease control (2.12). 3) As for the mean score according to school nursing phenomena related to environment, the score of the risk of accidents in classroom was highest as 2.68 points followed by the score of the risk of accidents around the school (2.66), maladjustment to school (2.62), the risk of accidents outside the classroom (2.43), inadequate learning environment (1.83), the risk of exposure to socially and physically harmful environment factors (La), and inadequate waste disposal (1.77). 4) This study tested the m n scores of questions corresponding to each school nursing phenomenon in order to see if there is a difference in the school nursing phenomenon among elementary, middle and high schools, and performed Duncan's posterior comparison for school nursing phenomena. A significant difference was found at p<.l. According to the results, school nursing phenomena found to be significantly different among elementary, middle and high schools was smoking and drug use (p<.05), which appeared more problematic in high school than in elementary school. phenomena such as inadequate eating habits, inadequate weight control, inadequate response to sex-related problems and inadequate waste disposal were also found to be statistically different at p<.l; however, according to the result of Duncan s posterior comparison, no difference was found among groups in improper eating habits and improper response to sex-related problems, and a significant difference was found between middle and high schools in inadequate weight control and inadequate waste disposal. Conclusion: Based on the results of this study, it is necessary to plan school health projects focused on the characteristic school nursing phenomena that had high scores and to develop and execute school healthe projects in accordance with the characteristics of elementary, middle and high schools. Considering that the scores of school nursing phenomena related to human behavior are high, it is necessary to introduce school health promotion projects in a systematic way.

만성관절염과 루푸스의 피로, 통증, 및 통증효능감에 관한 연구

임난영,이은영,양용숙,정순애,차경옥,이여진 대한류마티스 건강전문학회 2000 근관절건강학회지 Vol.7 No.1

This study was designed to distinguish by the characteristic difference and the degree of symptoms such as fatigue, pain, coping to pain, and efficacy on pain, and to offer descriptive data for nursing intervention for improving coping ability to pain along each characteristics of disease in chronic arthritis and systemic lupus erythematosus. The subjects were 135 outpatients in the hospital for rheumatic disease in H-University, Seoul. The data were collected by structural questionnaire, from April 29 to June 29, 1999. The results were that the fatigue score was high in the OA patients while the RA patients and SLE patients experienced middle range of fatigue score, but which was not statistically different. Although the RA patients felt higher pain than other diseases, they have well coped with their pain than the others. In efficacy on pain the SLE patients had higher score than others but all of the disease showed lower score. No statistically significant difference among the three croup was recorded in efficacy on pain. Therefore, pain management in the RA patients was primary nursing intervention because they felt severe pain and have well coped with pain while they had lower pain efficacy score than the others. It is also important that fatigue management and coping strategies on pain for the OA patients and SLE patients are specially supportive in the nursing intervention.