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CHO-KI 細胞의 Lysophospholipase 活性에 關한 硏究
張玹勖 영남대학교 자원문제연구소 1991 資源問題硏究 Vol.10 No.-
Lysophospholipase (E.C. 3.1.1.5) are widely distributed in prokaryotic and eukaryotic cells and are hypothesized to have an appreciable role in the metabolism of phospholipids by degrading potentially cytotoxic lysophospholipids generated by the action of phospholipase A₁/A₂. In the present study, lysophospholipase L₁(A₁) and lysophospholipase L₂(A₂) activities in chinese hamster ovary cell subclone(CHO-KI) that hydrolyze 1-acyl-lyso PC, 1-acyl-lyso PE, and 2-acyl-lyso PE were dectected and chracterized. These two enzymes were membrane associated enzymes and did not require Ca2+ ion for optimal activity. Lysophospholipase L₁show optimum pH 7.0, and lysophospholipase L₂ show broad optimum pH 6-9. These two enzymes were completely inhibited by -SH reagent, p-chloromercuriebenzoic acid (PCMB)and serine protease inhibitor, diisopropylfluorophosphate (DFP) and ionic and nonionic detergents. Substrate specificity of these two enzymes using 1-[14C] LPE, 1-[14C] LPC as substrate for lysophospholipase L₁, and 2-[14C] LPE, 1-[14C] LPC as substrate for lysophospholipase L₁, and 2-[14C] LPE, 1-[14C] LPC as substrate for lysophospholipase L₂, these two enzymes more preferentially hydrolyzed lyso PE than lyso PC. Lysophospholipase L₁ has transacylase activity. Abbreviation : EDTA, ethylene diamine tetra acetic acid Lyso PA, lysophosphatidic acid Lyso PS, lysophosphatidylserine PC, phosphatidylcholine PE , phosphatidylethanolamine Lyso PC, lysophosphatidylcholine Lyso PE , lysophosphatidylethanolamine
가죽나무 에타놀 추출물 및 luteolin-7-O-glucoside의phospholipase A2 저해활성
장현욱,김미화,황남경,홍태균,김윤경,정환기,양주혜,전철구,배기환,Pham Ngoc Thanh,손건호,김현표,강삼식 한국생약학회 2007 생약학회지 Vol.38 No.3
our continuing effort to investigate compounds having anti-inflammatory activity from natural products, Ail-anthus altissima was examined. Among six compounds isolated from Ailanthus altissima, Luteolin-7-O-glucoside (L7G) alongwith ethanol extract of Ailnathus altissima (EAa) were chosen to determine their inhibitory activity on secretory recombinantphospholipase A2s enzyme activity in vitro 2-V (IC50 of about 100g/ml) and cPLA2, (IC50 of about 59g/ml), while L7G showed strong inhibitory effect on sPLA2-A, V and cPLA2 with an IC50value of approximately 40M, respectively.Key wordsAilanthus altissima, Luteolin-7-O-glucoside, Phospholipase A2, inflamation... ... .... phospholipase A2(PLA2). ..... ...... arachidonic acid(AA) . lysophos-pholipid. .... . PLA2. .. ... AA. cyclooxy-genase(COX). lipoxygenase(LOX). .. ... ....... prostaglandins(PGs), thromboxanes(TXs) .leukotrienes(LTs). .. .... . .. ... lysophos-pholipid. .. .... .... ..... ... ...... .... platelet activating factor(PAF, ........ ). ..... PLA2. ... ...... .
장현욱,이보영,김은숙,이영은,Jang, Hyun-Wook,Lee, Bo-Young,Kim, Eun-Sook,Lee, Young-Eun 한국식품조리과학회 2016 한국식품조리과학회지 Vol.32 No.6
Purpose: This study was conducted to optimize the conditions of manufacturing the wet noodle added with Aloe powder. Methods: The I-optimal design of response surface methodology (RSM) was used for the optimization of added amount of Aloe powder, water content, and kneading time as the independent variables. The quality characteristics (color, texture, water absorption ratio, volume, turbidity) and sensory characteristics (color, flavor, taste, mouth feel, overall acceptance) were analyzed as dependent variables. Results: The lightness, redness, and yellowness of the Aloe noodle were all lowered with the addition of Aloe powder. All texture characteristics such as hardness, elasticity and chewiness of Aloe noodle showed the tendency to increase with increasing amount of Aloe added and kneading time. The water uptake and the volume expansion tended to increase with increasing aloe addition and water addition. As the kneading time increased, the dissolution of solids decreased and the turbidity tended to decrease. Aloe powder content influenced the color, flavor and taste of the noodles most among independent variables, and the mouthfeel of the noodle influenced by the water addition and the kneading time. Conclusion: Aloe noodle showed the best desirability with 3.03% of Aloe powder, 43.56% of water content and 13.06 min of kneading time by RSM analysis. Aloe noodles prepared under these optimized conditions are expected to be able to manufacture and utilize functional Aloe noodles by meeting the content of isobarbaloin, which helps the intestinal functional activity.
고초균 및 버섯 균사체를 이용하여 발효한 쌍별귀뚜라미 발효물의 단백질 및 무기질 성분 변화
장현욱,최지호,박신영,박보람 한국식생활문화학회 2019 韓國食生活文化學會誌 Vol.34 No.6
This study examined the changes in the protein and mineral composition of Gryllus bimaculatus fermented with Bacillussubstilis and the mycelia of Basidiomycetes. Normal Gryllus bimaculatus (S) and experimental group data obtained after aninoculation of Bacillus substilis (SC) (KACC 19623), Pleurotus eryngii (SP) and Cordyceps millitaris (SC) were compared. The crude protein content of the Gryllus bimaculatus (control) was 75.48%, but it decreased to 64.55, 54.32, and 63.53%after fermentation with SB, SP and SC, respectively (p<0.05). An analysis of the organic elements showed that the contentsof the carbon and nitrogen sources were also reduced after fermentation, and the most significant decrease was observedafter fermentation with SP. In SDS-PAGE, a 120 kDa and a 48 kDa protein of Gryllus bimaculatus were found. On the otherhand, protein bands faded after fermentation with SP and SC, respectively. Moreover, no visible band was observed afterfermentation with SB. According to amino acid analysis, the total free amino acid content increased 3.84 and 1.74 timesafter fermentation with SB and SP, respectively, compared to the corresponding baseline data. In contrast, it decreased by0.52 times after fermentation with SC. Among the essential amino acids found in crickets fermented with SB, the valine andisoleucine content was 3.57 and 2.64 times higher, respectively, than the recommended daily amount of essential aminoacids.
비중격 교정술 및 비갑개성형술이 수면 호흡장애 환자의 삶의 질에 미치는 영향
장현욱,정상식,남예모,김정수 대한이비인후과학회 2011 대한이비인후과학회지 두경부외과학 Vol.54 No.4
Background and Objectives Nasal obstruction is common in patients with sleep disordered breathing (SDB). Polysomnography (PSG) is a standard diagnositc tool for sleep disorders, but it can not evaluate the quality of life (QOL). We tried to assess the efficacy of the septoturbinoplasty on patients with SDB and nasal obstruction by using self reported measures that have been known as a tool for evaluating the general and disease specific QOL. Subjects and Method Thirty-five patients who had snoring and sleep apnea confirmed by PSG and symptomatic nasal obstruction caused by deviated nasal septum were enrolled. All patients underwent septoturbinoplasty to improve nasal obstruction. QOL was assessed before and 2 months after surgery. Medical Outcome Study Short Form 36 (SF-36) and Epworth Sleepiness Scale (ESS), Pittsburgh Sleep Quality Index (PSQI), Stanford Sleepiness Scale (SSS), Insomnia Severity Index (ISI), Beck Depression Inventory (K-BDI), Nasal Obstruction Symptom Evaluation (NOSE) scale were included in the measures. A statistical analysis was performed using a paired t-test. Results Snoring sound level, PSQI, ESS, ISI, and NOSE were improved significantly (p< 0.05). SSS, BDI and total SF-36 were improved but not significantly (p>0.05). Conclusion QOL in patients with SDB and nasal obstruction was improved by nasal surgery. Different kinds of self-reported measures could be more informative as they can provide pre and post treatment results with respect to psychosomatic effects in patients with SDB.