반코마이신 내성 장내구균 분리 환자에서 황색포도알균의 내성

김동욱,신선혜,김혜진,류선,이선희,장철훈,김영대,곽임수 대한감염학회 2004 감염과 화학요법 Vol.36 No.1

목적 : Vancomycin-resistant enterococci (VRE)로부터 S. aureus로의 vacomycin 내성 유전자의 전파는 오래전부터 예견되어져 왔으며 큰 걱정거리로 남아있다. 최근에 vanA 유전자를 가지고 있는 vancomycin-resistant S. aureus 2주가 미국에서 분리되었다. 이 연구의 목적은 VRE를 보균하고 있는 환자들에서 S. aureus 보균양상과 항균제 내성을 알아보는데 그 목적이 있다. 재료 및 방법 : 2001년 1월부터 2001년 12월까지 부산대학교병원에 입원하였던 환자들 중 임상검체에서 VRE가 분리된 환자 7명과 2001년 9월부터 12월까지 외과 중 환자실에 입원하였던 환자들 중 VRE 감시배양을 시행하였던 20명의 환자들을 대상으로 하였다. 환자들은 전비공, 액와부, 회음부, 직장에서 매주 면봉으로 검체를 채취하였다. 선별배지에서 검체를 배양하여 S. aureus를 분리하였고 원판 확산법을 이용하여 methicillin 감수성을 측정하였다. Broth microdilution 법으로 vancomycin MIC를 측정하였다. 결과 : 총 27명에서 73회의 방문과 292회의 배양이 시행되었으며 총 67주의 S. aureus가 분리되었다. 이중 64주(95.5%)가 methicillin 내성이었다. 전비공 MRSA 보균율은 VRE를 보균하고 있었던 환자는 19명 중 11명(58%)에서, VRE를 보균하지 않았던 환자는 8명 중 3명(37.5%)으로 VRE 보균자에서 보균율이 더 높았다. 총 64주의 MRSA의 vancomycin MIC의 분포는 0.5-2㎍/mL였으며 2㎍/mL를 초과하는 균주는 발견되지 않았다. 64주 중 vancomycin MIC가 1㎍/mL인 균주가 54주(84.4%)로 가장 많았으며 2㎍/mL 6주(9.4%), 0.5㎍/mL 4주(6.3%)의 순이었다. 결론 : VRE 환자에서 S. aureus의 보균율은 비보균자 보다 높았으며 분리된 균주들은 대부분 MRSA였다. VRSA 균주는 발견되지 않았다. Background : The transfer of vancomycin resistance from vancomycin-resistant enterococci (VRE) to Staphylococcus aureus has been predicted. The purpose of this study is to determine the prevalence and antibiotics resistance of S. aureus among patients colonized with VRE. Methods : Between January 2001 and December 2001, a prospective study was performed at Pusan National University Hospital on 27 patients. Surveillance swabs from nasal cavity, axilla, perineum, and rectum were obtained at weekly intervals. Methicillin susceptability of S. aureus was determined by oxacillin disk diffusion test and minimum inhibitory concentration (MIC) for vancomycin by microdilution broth test. Results : Total of 292 swab cultures were performed and 67 S. aureus isolates were collected. 64 isolates (95.5%) were resistant to methicillin. The prevalence of nasal MRSA carrier in 19 patients colonized with VRE was higher than that in 8 patients not colonized with VRE (58% vs. 37.5%). In 64 MRSA isolates, MIC (㎍/mL) for vancomycin ranged from 0.5 to 2. No isolates with MIC >2 ㎍/mL were observed. MIC of 1 ㎍/mL was shown (observed) in 54 isolates, 2 ㎍/mL in 6 isolates, and 0.5 ㎍/mL in 4 isolates. Conclusion : The prevalence of S. aureus with colonization of VRE is higher than that without colonization of VRE. Most of S. aureus isolates were resistant to methicillin. VRSA isolates were not observed.

우리나라 중·고등학생의 자살 생각 관련요인 분석

곽수진,이윤정,정혜선 韓國學校保健學會 2009 韓國學校保健學會誌 Vol.22 No.2

Purpose: By analyzing factors related to suicide ideation of Korean middle and high school students. the purpose of this study was to multi-dimensionally understand suicide ideation of Korean middle and high school students and arrange the plans to prevent suicide. Methods: This study used and analyzed the data on 'The 3rd (Year2007) Korea Youth Health Risk Behavior We based Survey (KYHRBWS)' conducted by the Korea Centers for Disease Control and Prevention. In this study we analyzed 74,698 respondents in total. SPSS 12.0 Program was used to analyze the survey. Descriptive statistics, Chi-Square Test and multivariate logistic regression were also used as research methods. Results: 23.8% of the Korean middle and high school students had suicide ideation during the last one year. Girls had higher possibility of suicide ideation by 1.51 times compared to boys. Students with poor academic achievement had more suicide ideation. In terms of health characteristics. students who perceived. themselves as not healthy. and students with short weekday sleeping hours. more drinking experience and more smoking experience had more suicide ideation than others. Furthermore. students who perceived themselves as overweight or obese had more suicide ideation than other students. In terms of mental characteristics, students with high level of stress. low level of happiness and depression had more suicide ideation. Conclusion : As a resuit of this study. we fa found out that the factor that had the biggest influence on suicide ideation of Korean middle and high school students was depression. Consequently, active supports and policies to decrease suicide ideation of Korean middle and high school students are necessary. Therefore, active interviews' education for suicide prevention, and periodical screening inspection should be introduced in schools

서울의 PPNG 발생 빈도(1991-1992)

김재홍,윤기범,박평원,김영진,전경민,김영태,김중환,곽호,구상완,송민석,유옥,지혜구,김동원,문상은,박영립,정승호,성범진,성순제,엄주용,황정열,이기홍,이주협,전태진 대한화학요법학회 1994 대한화학요법학회지 Vol.12 No.1

The prevalence of PPNG among pretreated gonorrhea cases isolated at the STD clinic of Choong-Ku Public Health Center in Seoul has been studied and reported annually since 1981. In 1991, 123 strains of N.gonorrhoeae were isolated, among which 58(47.1%) were PPNG. In 1992, 98 starains of N.gonorrhoeae were isolated, among which 51(52.0%) were PPNG. In all, 109(49.3%) strains were found to be PPNG among 221 strains isolated between 1991-1992. The prevalence of PPNG in Seoul showed increased tendency till 1989, thereafter, it has been stationary or slightly decreasing.

Revealed the Status of Microbial Diversity and Structure in Soil and Rhizosphere of Endangered Plant Species Cypripedium japonicum

Hye Sun Kim,Geun-Hye Gang,Eun-Hee Park,Youn-Sig Kwak 경상대학교 농업생명과학연구원 2017 농업생명과학연구 Vol.51 No.5

The Cypripedium japonicum is a species of orchid, distributed mainly in the temperate regions of the Northern Hemisphere. In Korea, C. japonicum is an extremely rare species and class I endangered plant. However, little is known about the plant-microbe interaction and its ecology, especially rhizosphere bacterial communities in terms of structure and diversity in C. japonicum. In this study, the diversity of bacterial communities was investigated in C. japonicum rhizosphere and bulk soil. Proteobacteria, Acidobacteria and Actinobacteria were the most abundant groups in both rhizosphere and bulk soil. Significantly Proteobacteria and Actinobacteria abundantly inhabited in the rhizosphere when compared with the bulk soil. Conversely, significantly more unclassified bacteria were detected in the rhizosphere soil than in the bulk soil. This work increases our understanding of the rhizosphere bacterial diversity on class I endangered plant, C. japonicum.

40℃ 48시간 에탄올발효 과정 중 일어나는 Saccharomyces cerevisiae KNU5377의 생리변화

Sun-Hye Kwak(곽선혜),Il-Sup Kim(김일섭),Kyung-Hee Kang(강경희),Jung-Sook Lee(이정숙),Ingnyol Jin(진익렬) 한국생명과학회 2011 생명과학회지 Vol.21 No.1

포도당 20%와 효모엑기스가 함유된 배지에서 40oC에서 48시간에 걸친 에탄올발효 과정 중에 일어나는 고온성 알코올 발효 효모균주 Saccharomyces cerevisiae KNU5377의 세포 내에서 일어나는 생리적 변화를 살펴보고자 했다. 그 결과, 이 발효 효모균주는 40℃ 48시간의 발효로써 11.4% alcohol을 생성하여, 고온발효인데도 불구하고 우수한 발효능을 가진 것을 확인할 수 있었다. 또한 12시간 발효과정동안 배지의 pH가 시작단계의 pH 6.0이 4.1로 내려갔으며, 이후 에탄올발효가 완성될 때까지 거의 변화하지 않고 이 값으로 유지되었다. 발효 12시간이 지나면서 세포막의 지방산의 조성은 불포화지방산인 C16:1 (palmitoleic acid)가 포화지방산인 C16:0 (palmitic acid)보다 1.5배나 증가하였으며, C18:1보다는 2배 가까이 증가하여 구성되어 있었다. 48시간 배양한 세포의 2차 전기영동(2-D)을 통한 세포내 단백질의 발현정도를 proteomics분석법으로 살펴본 바, phosphoglycerate kinase가 가장 크게 발현했음을 Mass Spectrometry를 통해 알 수 있었다. 그 뒤를 이어 adenylate kinase, Cys3p, Tdh3p, translational elongation factor 등이 크게 발현된 것을 알게 되었다. 이들은 직간접적으로 해당과정에 관여하는 인자들 이어서, 고온 장시간에 걸친 에탄올발효를 하는 이 발효 효모균주의 세포에게는 생존과 에탄올발효를 위하여 해당과정 관여 인자가 중요한 역할을 하고 있다는 것을 강력히 시사하였다. In this study, physiological changes in a thermotolerant yeast Saccharomyces cerevisiae KNU5377 cell exposed to 48-hour alcohol fermentation at 40℃ were investigated. After 12 hours of alcohol fermentation at 40℃, the C16:1 unsaturated acid of plasma membrane increased to 1.5 times more than the C16:0 saturated fatty acid, and to about 2 times more for the C18:1 unsaturated fatty acid. Fermentation at both 30℃- and 37℃- fermentation showed the same pattern as that done at 40℃. The pH of the alcohol-fermentation medium was reduced to pH 4.1 from a starting pH of 6.0 through the 12-hr fermentation and then maintained this level during the continuing fermentation. With the process of fermentation, the remaining glucose was reduced, but its amount remaining during the 40℃-fermentation was less reduced than those fermented at 30℃ and 37oC. In the study investigating the changing pattern of cellular proteins in the alcohol-fermenting cells, the SDS-PAGE and 2-D data indicated the most expressed dot was phosphoglycerate kinase, which is one enzyme involved in glycolysis. Why this enzyme was most expressed in the cells exposed to unfavorable conditions such as high temperature, increasing concentration of produced alcohol and long time exposure to other stress factors remains unsolved.

대장 게실증 임상양상에 영향을 미치는 인자에 대한 전향 연구

김선영 ( Sun Young Kim ),김유선 ( You Sun Kim ),김현태 ( Hyun Tae Kim ),권선옥 ( Sun Ok Kwon ),오명기 ( Myoung Ki Oh ),차인혜 ( In Hye Cha ),옥경삼 ( Kyeong Sam Ok ),곽철훈 ( Cheol Hun Kwak ),김진남 ( Jin Nam Kim ),문정섭 ( Jeong 대한소화기학회 2013 대한소화기학회지 Vol.62 No.2

목적: 평균수명의 증가에 따른 사회의 고령화와 식생활의 서구화 및 진단기법의 발전으로 우리나라 소화기관의 게실 빈도는 증가하는 추세이며 이에 따른 대장 게실의 임상양상 또한 후천적 영향에 의해 서구화될 것으로 생각된다. 이에 저자들은 대장 게실증을 진단받은 환자를 대상으로 대장 게실증에 영향을 미치는 후천적 인자를 알아보고자 하였다. 대상 및 방법: 2010년 5월부터 2012년 4월까지 인제대학교 서울백병원에서 시행한 대장내시경검사에서 게실이 관찰되었던 사람을 대상으로 하였으며, 설문을 통해 대상군의 나이, 체질량지수, 복부둘레, 운동량, 지방 섭취 정도, 흡연 및 음주의 정도를 조사하여 대장 게실의 임상양상과의 연관성을 알아 보았다. 결과: 연구 대상은 총 200명으로 나이는 54.9±11.9세(범위17-79세)였고 대장 게실증의 남녀비는 2.2:1이었다. 게실의 위치는 우측 대장이 83%였고 게실의 평균수는 4.07±3.9개였다. 게실 위치와 연관성을 보인 인자는 나이였는데 고령일 수록 좌측에 위치하였고(p=0.001), 게실의 수는 허리둘레와 유의한 연관성을 보였으며(partial correlation coefficient r`=0.143, p=0.047), 대장 게실염은 나이가 적을수록 증가하였다(p=0.002). 결론: 대장 게실의 위치는 고령일수록 좌측에 호발하였으며 게실 수는 복부비만과 연관성을 보였다. Background/Aims: The prevalence of colonic diverticulosis in Korea is increasing in conjunction with the adoption of western dietary pattern, extension of lifespan, and advances in diagnostic modalities. The clinical characteristics of colonic diverticulosis seem to be gradually becoming similar to those of Western societies. Therefore, factors associated with the clinical characteristics of colonic diverticulosis in Korea were investigated. Methods: The data of 200 patients diagnosed with colonic diverticulosis using colonoscopy between May 2010 and April 2012 at Inje University Seoul Paik Hospital (Seoul, Korea) were prospectively collected. Clinical parameters acquired through a questionnaire include age, body mass index, waist circumference, exercise, diet, smoking, drinking habits, etc. Correlation between these factors and the clinical features of diverticulosis were analyzed. Results: Mean age of the patients was 54.9±11.9 (range 17-79) years and male to female ratio was 2.2:1. Most diverticula were located on the right side of the colon (83%) and the mean number of diverticulum was 4.07±3.9. Factor associated with the location of diverticulum on the left side was age (p=0.001). There was a positive correlation between the waist circumference and the number of diverticulum (partial correlation coefficient r`=0.143, p=0.047). Diverticulitis occurred more frequently in younger patients than in older patients (p=0.002). Conclusions: Colonic diverticulosis in older patients is found more frequently on the left colon, and the number of diverticulosis is associated with central obesity. (Korean J Gastroenterol 2013; 62:97-103)

가짜뉴스의 생산과 전파, 그리고 규제

곽선혜(Sun-Hye Kwak),이성욱(Sung-Wook Lee) K교육연구학회 2024 사회과학리뷰 Vol.9 No.1

가짜뉴스는 시대 상황의 가장 자극적인 문제를 드러내고 있다. 논쟁이 증가함에 따라 학문적 연구가 확대되고 있지만 다양한 하위 주제들이 복잡하게 얽혀 있는 까닭에 많은 수의 연구 결과를 일일이 살펴보지 않는 이상 가짜뉴스에 대한 종합적인 이해를 얻기 어려운 실정이다. 이에 본 논문은 하나의 연구 결과물을 통해 가짜뉴스의 생산과 전파, 규제에 대한 거시적인 이해를 얻는 것을 목표로 한다. 먼저, 가짜뉴스의 개념과 범위에 대한 현재의 주요 논의와 쟁점을 조사하고, 가짜뉴스의 발생 원인과 전파 과정을 검토해 미디어 환경의 변화와 인간 심리가 어떻게 가짜뉴스의 전파에 영향을 미치는지를 확인하였다. 또한 가짜뉴스의 규제에 대한 쟁점적 논의를 조사하고, 규제는 표현의 자유권 차원에서 최소화되어야 한다는 점과 기술적 규제 등 대안적 방식으로 이뤄지는 것이 바람직하다는 점을 제시하였다. 이상의 논의를 통해 가짜뉴스에 대한 거시적인 이해를 제공함으로써 가짜뉴스를 효과적으로 관리하고 규제하는 방법을 찾는 연구 분야, 특히 기술적 규제의 새로운 길을 찾는 융합 학문 분야의 이론적 검토를 효율화하는 데 본 글이 기여할 수있기를 바란다. Fake news reveals the most stimulating problem of the times. Academic research is expanding as controversy increases, but it is difficult to obtain a comprehensive understanding of fake news unless a large amount of research results are examined one by one due to the complex intertwining of various sub-topics. Therefore, this paper aims to gain a macroscopic understanding of the production, dissemination, and regulation of fake news through a single research result. First, the current major discussions and issues on the concept and scope of fake news were investigated, and the causes and propagation processes of fake news were reviewed to confirm how changes in the media environment and human psychology affect the spread of fake news. It also investigated the controversial discussion on the regulation of fake news and suggested that the regulation should be minimized in terms of freedom of expression and that it is desirable to be implemented in an alternative way, such as technical regulation. It is hoped that this article can contribute to streamlining the theoretical review of the research field, especially the convergence academic field, which finds new ways to effectively manage and regulate fake news by providing a macroscopic understanding of fake news through the above discussion.

폐렴과 급성 호흡부전으로 나타난 흉막의 고립성 섬유성 종양

박혜선 ( Hye Sun Park ),곽현정 ( Hyun Jung Kwak ),박동원 ( Dong Won Park ),구태연 ( Tai Yeon Koo ),김혜영 ( Hye Young Kim ),박소연 ( So Yeon Park ),안성은 ( Seong Eun Ahn ),김상헌 ( Sang Heon Kim ),김태형 ( Tae Hyung Kim ),손장원 대한결핵 및 호흡기학회 2008 Tuberculosis and Respiratory Diseases Vol.65 No.4

Solitary fibrous tumors of the pleura (SFTPs) are relatively rare tumors that originate from the mesenchymal cells of the submesothelial tissue of the pleura. Patients with SFTPs are often asymptomatic, while some patients experience pleuritic chest pain, cough and/or dyspnea. We report here on a case of SFTP, and the patient presented with septic shock and respiratory failure that required mechanical ventilation. A 68-year-old woman was admitted for the evaluation of her dyspnea and generalized edema. Chest imaging studies showed an 18 cm-sized voluminous mass occupying the right thoracic cavity with anterior displacement of hilar structures and atelectasis of the right lung. Immediately after admission, she developed pneumonia and septic shock that required antibiotics and mechanical ventilation. She displayed a partial response to medical treatment, and then complete excision of the tumor was performed and the pathologic examination revealed benign SFTP. Afterward, she fully improved without evidence of recurrence until now. (Tuberc Respir Dis 2008;65:334-338)

HIF2α/EFEMP1 cascade mediates the effects of hypoxia on breast cancer stem cell hierarchy

Ji-Hye Kwak,Jeong-Seok Nam,In-Sun Hong,Soo-Rim Kim,Kyu-Beom Jang,Hye-Jin Dong,Ye-Won Son,Hae-In Song,Na-Hee Lee,Se-Ra Park,Hyun-Soo Eum 한국실험동물학회 2015 한국실험동물학회 학술발표대회 논문집 Vol.2015 No.2

에너지플러스에 의한 반투과성 태양광 적용공간의 에너지성능 분석

문선혜(Sun-hye Mun),정영선(Young-Sun Jeong),강정아(Jeonga Kang),곽영훈(Younghoon Kwak),허정호(Jung-Ho Huh) 한국태양에너지학회 2020 한국태양에너지학회 학술대회논문집 Vol.2020 No.10