한방 이화주의 미백 및 피부 주름 개선 효과

이상진(Sang-Jin Lee),권이영(Yi-Young Kwon),조성원(Sung-Won Cho),권희숙(Hee-Suk Kwon),신우창(Woo-Chang Shin) 한국식품영양과학회 2013 한국식품영양과학회지 Vol.42 No.4

본 연구에서는 한방이화주의 피부 생리기능 활성을 알아보기 위해 70% EtOH 추출물의 피부 미백, 주름 개선 및 항염증 효과를 조사하였다. HEE은 tyrosinase 활성 억제 및 tyrosine을 기질로 melanin이 형성되는 pathway에 관여하는 주요한 인자인 TRP-1과 TRP-2를 저해하는 작용 기전을 통해 피부 색소침착의 주요 원인 물질인 melanin 생합성을 농도 의존적으로 저해하는 것을 확인하였다. HEE은 피부진피 내 피부 탄력을 유지하는 elastin을 분해하는 효소인 elastase의 활성을 저해하였고, 피부의 keratinocyte가 생성분비하는 MMP-2와 MMP-9의 단백질 발현과 proteolytic 활성을 억제하여 노화에 따른 피부 주름 생성 억제할 수 있는 가능성을 확인하였다. 또한 세포 독성 없이 LPS에 의해 유도된 염증 반응을 50% 저해하는 HEE의 농도(IC50)는 24.9 μg/mL이며, 50 μg/mL 농도로 처리하였을 때 염증 반응 저해 효과가 70%로 높은 효과를 가지는 것을 확인하였다. 이상의 결과를 종합하면 HEE의 피부 미백, 주름 개선 및 항염증에 우수한 효과를 나타내고 있으므로 기능성 화장품의 주요한 소재로 이용 가치가 높을 것으로 사료된다. Extrinsic skin aging is characterized by the loss of skin tone and resilience, irregular pigmentation, and deep wrinkles. The aim of this study was to investigate the effects of Ehwa Makgeolli containing oriental herbs (Glycyrrhiza uralensis Fisch., Lycium chinense MILL., Morus alba L., and Saururus chinensis Baill) on skin whitening and wrinkling in human skin cells. We prepared Makgeolli extracts (HEE) with 70% ethanol. HEE significantly inhibited in vitro mushroom tyrosinase activity and reduced the cellular and secreted melanin content of mouse melanoma melanocytes (B16F1 cells). HEE down-regulated the protein expression of tyrosinase related protein (TRP)-1/-2, a key player in melanogenesis. Treatment with HEE in human keratinoctyes (HaCaT cells) inhibited the proteolytic activities of matrix metalloproteinase (MMP)-2/-9 in a dose-dependent manner and dramatically reduced the expression of MMP-2/-9. In addition, HEE attenuated lipopolysaccharide (LPS)- induced nitric oxide production in murine macrophages (RAW264.7 cells). These results indicate that HEE may be a great cosmeceutical ingredient for its whitening, anti-wrinkle, and anti-inflammatory effects.

"하고초(夏枯草)"의 생약학적 연구

황명석,조창희,박종희,Whang, Myung-Suk,Cho, Chang-Hee,Park, Jong-Hee 한국생약학회 2000 생약학회지 Vol.31 No.2

'Ha Go Cho (夏枯草)' is one of the Chinese crude drugs used mainly as a diuretic. With regard to the botanical origin of 'Ha Go Cho', it was reported by Su et al. that those from China were originated from the fruited spica of Prunella vulgaris L. of Labiatae. It was, however, for the herba or spica of Prunella vulgaris L. var. lilacina Nakai from Korea. According to survey of markets in Korea, most of the materials collected in the markets seemed to be originated from Prunella plant, while some seemed to be Thesium plant of Santalaceae. To clarify the botanical origin of 'Ha Go Cho', the anatomical characteristics of Prunella vulgaris L. var. lilacina Nakai and Thesium chinense Turcz. were studied. As a result, it was clarified that some 'Ha Go Cho' from Korea were the herba or spica of Prunella vulgaris var. lilacina, whereas some others were the herba of Thesium chinense.

다발성 대장암 간전이 환자의 간절제를 위한 새로운 시도

주종우,김형철,임철완,신응진,조규석,유기원,송옥평,홍대식,박성진,조준희,이혜경,김희경,권계원,고은석 순천향의학연구소;Soonchunhyang Medical Research Institute 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

Multiple bilobar liver matastases (MBLM) are the main cause of low resectability in the colon cancer liver metastases. The authors experienced one case of initially non-resectable colon cancer liver metastases. He was curatively and safely treated with a two-stage hepatectomy using the new method of future remnant liver volume growing. A 54-year-old man was referred to our department with the sigmoid colon cancer combined with MBLM, which were checked in two small metastatic lesions in the left lobe and five large sized lesions in the right lobe in the computed tomogram (CT). A laparoscopic assisted anterior resection was primarily performed. We performed the 1^(st) stage hepatectomy 3 weeks after the colon resection. Intra-operative Ultrasonogram (US) found 9 small superficial metastatic lesions in the left lobe. All that lesions were completely removed by non-anatomical wedge resection. An occlusion ballon catheter was placed in the right portal vein through a small branch of the inferior mesenteric vein at that time. The future remnant liver volume was sufficiently increased 3 weeks after the 1^(st) hepatectomy. A right hepatectomy was safely performed 22 days after the 1^(st) hepatectomy. The patient received a regional chemotherapy (interleukin2 based immuno-chemotherapy through hepatic artery) for 4 months, then received 9 cycles of systemic chemotherapy (biweekly Oxaliplation, leucovorin, plus 5-fluorouracil) without any recurrence evidence.

땅비싸리와 좀땅비싸리의 형태적변이 및 분류

조숙경,최병희,김주환 대전대학교 이과대학 기초과학연구소 1997 自然科學 Vol.- No.-

동북아시아에 분포하는 땅비싸리와 좀땅비싸리의 분류학적 한계를 규명하기 위하여 형태적 변이와 염색체수가 조사되었다. 땅비싸리의 이명으로 취급되어 왔던 좀땅비싸리는 소엽의 모용, 꽃의 크기, 화분형태, 지리적 분포 등에 있어 땅비싸리와 뚜렷한 차이를 보였으며, 또한 염색체수가 4배체(2n=32)로 나타나, 2배체(2n=16)인 땅비싸리와 차이를 보였다. 땅비싸리와 좀땅비싸리 11 집단을 대상으로 19개의 외부형태 형질에 대한 주성분분석을 실시한 결과 두 종은 제1주성분에 의해 뚜렷이 나누어 졌는데, 전체분산에 대한 제1주성분의 기여율은 63.2%이었으며, 제1주성분은 소엽 표면의 털과 화서의 길이 등과 높은 상관성을 보였다. 이러한 결과로부터 좀땅비싸리는 땅비싸리와는 구별되어 독립된 종으로 처리되었다. 한편 큰땅비싸리는 땅비싸리의 변이의 한 형태로 판단되므로 땅비싸리의 이명으로 처리되었다. 각 종의 지리적 분포에 대한 분류학적 의의가 염색체수 변이 등과 비교논의 되었다. The morphological analysis and cytological study of Indigofera kirilowii Maxim. and Indigofera korean Ohwi were undertaken to assess the numbers and ranks of taxa that usefully could be recognized. I, koreana, previously recognized as I. kirilowiii, was separated from I. kirilowii in hairiness on leaflets, flower size, pollen exine sculptures, geographical distribution and chromasome number. Chromosome count of 2n=32 (tetraploidy) from I. koreana was obtained for the first time. The results of principal components analysis (PCA) on a matrix of 11 populations of Korea by 19 morphological characters indicated that two species could be recognized by the first principal component, contribution of which for the total variance is 63.2%. Our results supported that I. koreana could be clearly distinguished from I. kirilowii. Since the quantitative differences between the two varieties, I. kirilowii var. coreana and var. Kirilowii, were smaller, a nomenclatural synonymy was developed for them, ie, var. kirilowii.

젊은 성인 남자의 흡연과 혈청 지질과의 관련성

조선,류소연,박종,강명근,김은숙,김형철,박광희,윤혜은,노희송 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.2

Objectives: This study was performed to find the association of cigarette smoking and blood lipid profiles in healthy young adults. Methods: The data was collected by a self-administered questionnaire and health examination in 489 professional soldiers from 6 to 12 May, 2004. The status of cigarette smoking was classified by non-smoking, ex-smoked, ≤4.9, 5-9.9, and ≥10 pack-years. The statistical analysis were used by chi-square test, analysis of variance and analysis of covariates. Results: In status of subjects cigarette smoking, current smoking, ex-smoked, and non-smoking were 71.8%, 11.2%, and 17.0%, respectively, In distribution by amount of smoking, non-smoking, ex-smoked, ≤4.9 pack-years, 5-9.9 pack-years, and ≥10 pack-years were 17.0%, 11.2%, 37.4%, 21.3%, and 13.1%, respectively. At other confounding variables were controlled, total cholesterol and LDL-cholesterol were increased significantly when amount of smoking was increased. However, triglyceride and HDL-cholesterol did not showed statistically significant differences, Conclusion: Smoking has relevant to total cholesterol and LDL-cholesterol and it is need to perform the further study for finding how blood lipid profiles are affected its level by cigarette smoking and to find an influence of cigarette smoking on cardiovascular disease.

Culex pipiens pallens 모기의 난황단백질 합성에 대한 Hormone의 영향

조정래,박영민,강석희 성균관대학교 기초과학연구소 1988 論文集 Vol.39 No.2

Culex pipiens pallens모기의 난황단백질 합성에 대한 brain hormone과 β-ecdysone의 영향에 대하여 조사하였다. 흡혈하지 않은 모기에게 β-ecdysone을 주사하였을 때 주사 후 12시간에 최대의 vitellogenin 합성을 나타내었다. 흡혈 후 decapitation 이나 ligation된 모기중 maturing ovary를 가진 모기의 vitellogenin 합성율은 decapitation이나 ligation이 지연될수록 증가하였다. 흡혈 후 decapitation이나 ligation된 모기중 maturing ovary를 가진 모기의 vitellin level은 decapitation이나 ligation이 지연될 수록 증가하였다. 이 결과는 C. pipiens 모기의 난황단백질 합성은 brain hormone과 β-ecdysone이 조절할 가능성을 제시해 주고 있다. Injection of β-ecdysone into unfed Culex pipiens pallens(Diptera: Culicidae) females stimulated incorporation of ^3H-leucine into proteins by the fat body in vitro. The rate of incorporation began to rise by 6 hours and reached at a maximum at 12 hours after ecdysone injection. Blood fed decapitated females produced maturing and arrested ovaries. The fat bodies of females with arrested ovaries failed to synthesize proteins, while fat bodies from females with maturing ovaries produced vitellogenin even when the females were decapitated within few minutes after a blood meal. The yolk deposition into the ovaries for females with maturing ovaries was not affected by decapitation.

일반학교에 배치된 특수교육보조원의 역할수행 어려움에 대한 질적 연구

조연희,이숙정 국립특수교육원 2008 특수교육연구 Vol.15 No.2

특수교육보조원 제도가 본격적으로 시행되고 있지만, 당사자들이 교육현장에서 겪는 역할갈등, 어려움 등에 대한 논의는 피상적 수준에 머무르고 있다. 구체적인 교수학습 현장에 투입되어 나름의 교육활동을 하고 있는 이들이 느끼는 어려움에 대한 심층적 고찰은 이 제도가 제대로 정착하고 발전하기 위한 초석을 마련하리라 본다. 본 연구는 특수교육보조원의 역할수행 어려움을 당사자를 대상으로 반구조화된 질문지를 기준으로 3가지 측면에서 심층면담하였다. 교수-학습 지원 상황에서는 눈치, 역할지시의 부재로 인한 혼란, 지식의 부족으로 어려움, 인간관계에서는 무시, 역할에 대한 잘못된 인식, 차별적 관심과 대우, 어중간한 중간입장으로 어려움이 있었으며, 또한 환경적인 부분에서는 열악한 고용조건, 나만의 공간 부족, 부족한 연수, 불투명한 소속, 육체적 노동으로 인해 어려움을 느끼고 있는 것으로 나타났다. The teacher-aid-system in the field of special education has emerged as a developmental system for effective inclusion from March 2003 in South Korea. The role-conflict and difficulties of paraprofessional in special education have almost discussed in survey inquiry. But the subjective feeling about role-conflict of paraprofessional in the real field of teacher-aid-system could be different from the results through general survey inquiry. The purpose of this study was to analyze and interpretate qualitatively what are the role-conflict and bottlenecks of paraprofessional in the real field. They are asked with half-structured questionary in interview in depth. The results of this study were that: (a) in terms of personal relations in the school: the paraprofessional confronts with the problems like neglecting, wrong perception about their roles, discriminative treatment and position hanging in midair, (b) As concerns about situation for learning-support, they should often see how the winds blow between teacher and students. Furthermore they are diffused cause of insufficient instructions from the teachers, how the paraprofessional should support the student with special educational needs. In these case, they blushed at their ignorance. (c) About the working condition: they are suffering with much inconvenience like bad salary, poor working surrounding, lack of own-recreative space and hard-physical working.

중등 과학교사 양성 교육과정과 교수내용지식 연구 동향의 탐색

조희형,조영신,권석민,박대식,강영진,김희경,고영자 이화여자대학교 교과교육연구소 2006 교과교육학연구 Vol.10 No.2

과학교사의 양성을 위한 교육과정 또는 그 프로그램의 주된 목적은 바람직한 과학교사가 지녀야 할 전문적 자질을 함양시키는 데에 있다. 그러나 우리나라의 과학교사 양성을 위한 프로그램 또는 사범대학 과학교육학 계열 교육과정에는 과학교사 교육의 본성과 그의 자질에 관한 연구의 결과가 충분히 반영되어 있지 않다. 이 연구는 자질기반 과학교사 양성 교육과정의 개발 틀, 교직의 전문성과 전문성발달기준, 우리나라의 과학교사 양성 교육과정 및 프로그램에 관한 문헌을 조사·분석한 다음, 그 결과로부터 우리나라의 과학교사 양성을 위한 교육과정의 개발 방향을 도출하고 그 모형을 탐색할 목적에서 수행하였다. 이 연구에서는 과학교사 양성 교육과정을 그 과목이나 지식기반 영역에 따라 교수내용알기(PCKg), 교수구성단위(TPCs), 내용표상화(CoRe)와 교수전문경험자료(PaP-eRs), 그리고 교수상황지식(PCxK)을 단위로 이용하여 구성할 것을 강조하였다. 또한 과학교사의 전문적 자질의 함양에 목적을 둔 교육과정 또는 그 프로그램의 과학교육 및 과학과 관련된 과목으로 일반과학, 심화과학, 일반과학교육학(과학교육론, 과학 교수-학습 포함), 과학 교육과정 및 평가, 과학교육 시설과 자원, 과학교육 실습, 과학교육 연구를 제안하였다. The subjects and their contents comprising the curricula for secondary science teacher education should be selected/organized to assist pre-service teachers to gain understandings of knowledge ba ses and to help them acquire professional teaching competences. However, the nature of science teacher education has not been reflected on the Korean science teacher education programs. The research was performed to review the literature on teachers' competencies including PCK(pedagogical content knowledge), teaching as profession, curricula or programs for secondary science teacher education, and, based on the results, to suggest curricular subjects and their contents for secondary science teacher education in Korea. The research emphasizes that the subjects and their contents for secondary science teacher education should be developed by the use of PCKg(pedagogical content knowing), TPCs(teacher professional constructions), CoRe(content representation) and PaP-eRs(pedagogical and professional-experience repertoires) as constitutive units, depending on the subject areas or contents. Also suggested in this research are the knowledge bases assumed to be necessary for the development of secondary science teachers' professional qualities, which include general sciences, enrichment sciences, general science education, science education curricula and evaluation, facilities and resources for science education, teaching practices, research in science education.

돈사폐수를 이용한 Polyhydroxyalkanoates의 생산

조경숙,박창호,류희욱 이화여자대학교 환경문제연구소 1997 이화환경연구 Vol.1 No.-

돈사폐수를 발효기질로 이용한 생분해성 고분자, polyhydroxyalkanoates(PHA) 의 생산이 가능하였다. 균주로는 질소 혹은 인 등의 비탄소 영양원의 제한 없이도 PHA 의 생산이 가능한 Azotobacter vinelandii UWD 를 이용하였고 돈사폐수배지에서의 PHA 생산특성을 glucose 합성배지에서의 특성과 비교하였다. A. vinelandii UWD 균주는 탄소원으로 glucose와 alkanoates 가 함유된 배지에서 alkanoates를 우선적으로 소비하여 주로 생장을 하고, glucose소비단계에서는 생장보다는 PHA를 생산하는 diauxic growth 를 하였다. 돈사폐수 원액을 배지로 사용할 경우 UWD 균주의 건조균체량과 PHA 함량이 낮았는데(0.91g/L, 37wt%), 이는 돈사폐수 중에 함유되어 있는 acetate, propionate, butyrate 및 valerate등과 같은 높은 농도의 alkanoates가 세포생장을 저해하기 때문이었다. 증류수로 50% 희석한 배지(총 생산 균체 건중량 2.02g/L)를 사용하는 것이 바람직한 것으로 밝혀졌다. 50% 희석돈사폐수 배지에 glucose를 3% 첨가하면 건조균체량은 9.40g/L로 현저하게 증가하였으며, 균체내 PHA 함량은 58.3%, PHA 생산속도는 0.11g/(L·h)이었다. Glucose 합성배지에서 합성된 PHA는 poly-β-hydroxybutyrate(PHB)의 형태이었고, 돈사폐수 배지로부터는 copolymer인 poly-β-hydroxybutyrate-co-β-hydroxyvalerate, P(HB-co-HV)를 2-8mol%합성할 수 있었다. 돈사폐수배지를 이용하여 PHA를 생산하는 동안 배지의 염도를 대략 50% 정도 제거가 가능하였다. Biodegradable polymer, polyhydroxyalkanoates(PHA), could be produced using swine wastewater (SW) as a fermentation substance. Azotobacter vinelandii UWD was able to produce PHA without limiting non-carbon nutrients such as nitrogen and phosphorous. Production characteristics of strain UWD in SW medium were compared with those in glucose synthetic medium. In the synthetic medium containing glucose and alkanoates as carbon sources. A. vinelandii UWD grew diauxically, first by using alkanoates and then by using glucose to produce biomass PHA. Using raw SW medium, cell mass production was small (0.91g/ L) and PHA content was 37wt% because cell growth was inhibited by alkanoates such as acetate, propionate, butyrate, and valerate contained in SW. Use of two-fold diluted SW as fermentation medium was desirable for PHA production. When 3% of glucose was added to the two-fold diluted SW, dry cell mass increased to 9.40g/L, and the PHA content and PHA production rate were 58.3wt% and 0.11g(L·h), respectively, When grown in SW media A. vinelandii UWD formed PHA copolymers containing β-hydroxybutyrate and β-hydroxyvalerate (HV), and HV content in the copolymer was 2-8mol% whereas homopolymer of poly-β-hydroxybutyrate was produced in the glucose synthetic medium. Approximately, 50% of salinity in the medium was reduced after PHA production using SW medium.

아말감 충전후 두발 및 소변내 수은 함량에 관한 연구

조명숙,송근배,윤희숙 대구보건대학 1992 대구보건대학 論文集 Vol.13 No.-

The purpose of the present study was to determine the mercury in human scalp hair and urine from amalgam fillings, sixty-five subjects were examined their oral status, alginate impression were taken to make dental arch stone models and calculated their surface area of amalgam fillings. The 28 control subjects had no amalgam filling and 37 compare subjects had an average of 12 surfaces of amalgam. The morning urine and scalp hair were collected from all subjects and samples were digested in acid digestion bomb(Parr Instrument Company, U.S.A.) The mercury contents were determined with atomic absorption spectrophotometer(Model 551, IL Co., U.S.A.) and atomic vapor accessory (Model 440, IL Co., U.S.A.) . The results obtained were as follows ; 1. The urinary mercury contents of control group(no amalgam in oral cavity) were 2±10.0㎍/g. creatinine/L and compare group(amalgam filling) were 16.7±16.0㎍/g. creatinine/L(p<0.05). 2. The scalp hair mercury contents of amalgam filling group(3.29±1.03㎎/L) were slightly higher than those of the amalgam free group(3.04±1.11㎎/L) but not significant difference(p>0.05). 3. The linear correlation coefficient was 0.3490 and significantly different between mercury content of scalp hair and amalgam surface area(p<0.05), but had no significant difference between urinary mercury contents and amalgam surface area(r=0.0934, p>0.05).