정상과 갑상선 종양조직에서 사람 IGF-I 유전자의 발현

김성운,장현하,박상미,김덕윤,우정택,양인명,김진우,김영설,김광원,고석환,홍성화,최영길 경희대학교 유전공학연구소 1993 遺傳工學論文集 Vol.5 No.-

Many of the growth-promoting properties of growth hormone(GH) are mediated by insulin-like growth factor-I(IGF-I), a highly conserved circulating 70-amino acid peptide. Recent studies have shown that multiple mechanisms influence IGF-I gene expression, including transcription from two promoters, alternative RNA splicing, and variable polyadenylation. In thyroid tissue, thyroid stimulating hormone(TSH) and IGF-I are the most possible candidates for follicular cell proliferation and hypertrophy. Actually IGF-I had autocrine and paracrine effect for tissue growing. We prepared thyroid tumor tissue mRNAs using single step method for detecting IGF-I levels according to different tissues, i.e., thyroid adenoma or papillary thyroid carcinoma. We used Northern blot analysis for IGF-I mRNA and RNase protection assay (RPA) for IGF-I transcription start sites. For Northern blot, we used whole human IGF-I cDNA as a DNA probe and for RPA, we used IGF-I exon 1 containing noncoding promoter 1 as a riboprobe. We got good RNA bands from Northern blot analysis around 1 kb (IGF-IA) and 7.5 kb (IGF-IB) region. To clarify the amount of both IGF-IA and IB mRNAs, we measured autoradiographied signal of IGF-I mRNAs bands using densitometer. In IGF-IA signals, there's no change among liver and thyroid tissues, but in case of IGF-IB mRNA bands, the signal was markedly increased in thyroid carcinoma tissues than that of normal thyroid tissue (85% vs 14%). In the study of RPA, all thyroid tissues used the same transcription start sites as those of liver's. We concluded that that this different regulation of IGF-I mRNA was originated from tissue specificity. That meant some tissue specific transcription factor/s were related to tissue IGF-I expression.

『素問· 逆調論』에 대한 硏究

김화창,김종호,천상묵,서대선,김명수,금경수 한국전통의학연구소 2002 한국전통의학지 Vol.12 No.1

A Study on the Youkjoron(逆調論) of the So Moon(素問) one of the classic book of oriental medicine, was carried out analytically for the right comments. The principal ideas abtained summerized as follows: The unbalance between Eum(陰) and Yang(陽) The unbalance between Su(水) and Wha(火) The Yuk Ga(肉苛) which is caused by the unbalance of the Young Wi(營衛) The Asthma(喘息) which is caused by the unbalance of the Viscera(臟腑)

휫스턴 브리지를 응용한 기능성 스마트 콘크리트의 개발

성원경,김이성,김화중 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1(구조계)

Various structural materials have been used in construction projects using stones, concretes, and steels materials. Among of these projects, concretes may use widely because concretes have high compressive strength, and comparatively easy maintenance and management. Reinforced concrete Buildings will be deteriorated as time passed. These problems will be accelerated by propagation of cracks. In order to manage such cracks, time, efforts and expense are required. In this study, leakages of fluorescence and adhesive material were investigated using glass sensors that were embedded in a model beam and column. In addition, currents in glass pipe sensor were observed to find leakage of liquid in glass pipes. Progressive cracks were generated by fracture of glass pipe sensor. In this investigation, a reinforcement clothing system was wrapped for a glass pipe sensor. The glass pipe sensor that can make control and reinforce cracks simultaneously.

휫스턴 브리지를 응용한 스마트 콘크리트의 개발

김이성,성원경,김화중 대한건축학회 2005 大韓建築學會論文集 : 構造系 Vol.21 No.3

Various structural materials have been used in construction projects using stones, concretes, and steels materials. Among of these projects, concretes may use widely because concretes have high compressive strength, and comparatively easy maintenance and management. Reinforced concrete Buildings will be deteriorated as time passed. These problems will be accelerated by propagation of cracks. In order to manage such cracks, time, efforts and expense are required. In this study, leakages of fluorescence and adhesive material were investigated using glass sensors that were embedded in a model beam and column. In addition, currents in glass pipe sensor were observed to find leakage of liquid in glass pipes. Progressive cracks were generated by fracture of glass pipe sensor. In this investigation, a reinforcement clothing system was wrapped for a glass pipe sensor. The glass pipe sensor that can make control and reinforce cracks simultaneously.

콘크리트의 應力-變形關係에 關한 實驗的 硏究 : 鋼纖維 콘크리트에 關하여 About steel fiber concrete

金和中,權寧煥,李尙宰,全明熏 경북대학교 산업기술연구소 1990 産業技術硏究誌 Vol.18 No.-

The factors adopted in this experiment are volume fraction and the diameter and aspect ratio of fiber. The levels of factors are determined by existing studies. The effects of the diameter and mixing volume fraction and the aspect ratio for maximum strength, strain and tangent modulus are analyxed based on the linear multiple regression and we obtained regression equations as follows ; Maximum stress : σ_max=298.59820+95.78361φ-6.36043V_f+0.62067Ar Maximum strain at maximum stress : ε_max=0.021336+0.15746φ+0.05519V_f+0.00181Ar Tangent modulus : E=208209.80+87132.84φ-17286.69V_f-464.9181Ar where φ : diameter V_f : volume fraction Ar : Aspect Ratio

세립형 고강도 구조용 강재를 이용한 압축재의 실험적 평가

김요숙,임성우,장인화 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2(구조계)

As for the experimental check, stub and long column tests were carried out. Comparison tests for local and global buckling strength evaluation of box-type and H-shaped welded columns were performed with variable width-thickness ratio. Two kinds of steels were used for tests: High strength steel as a material with yield plateau and same grade steel as a material with continuous yielding. All of the specimens were satisfied with design codes regardless of exceeding the design limits. It means that the current width-thickness ratios may apply to both of the material with yield plateau and that with continuous yielding.

국내 분리 방선균의 항균활성 특성

김소연,박동진,권오성,임채영,김판경,이상화,김창진 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

제주도지역, 충청남도 서해안지역, 강원도 및 충청북도 자연 동굴지역, 그리고 충청북도 청원군 등지에서 채집한 205점의 토양시료로부터 분리된 총 1,322주의 방선균을 대상으로 Eschericia coli KCTC 1924, Salmonella typhimurium KCTC 1926, Staphylococcus aureus KCTC 1926, Staphylococcus aureus KCTC 1928, Candida albicans KCTC 1940등 5종의 시험균주에 대하여 항균활성을 조사하였다. 액체배양을 위해서는 G.S.S(glucose-soybean meal-starch) 배지와 fish meal 배지등 2종류의 배지를 사용하였으며 그 결과를 균분리원 지역별, 배지별, 시험균주별, 그리고 방선균 종류별로 비교하였다. It is well known that actinomycetes would be useful for screening of biologically active compounds. Not only to isolate diverse actinomycete strains but to ferment those strains effectively would be important. Seven hundred and forty six strains were isolated from Cheju province, 216 strains were from Chungnam province, 158 strains were from the natural caves at Chungbuk and Kangwon provinces and 202 strains were from Chungwon area at Chungbuk province. All of these 1,322 strains were fermented on a small scale using two different media and tested for their antimicrobial activities against four bacterial strains and one yeast strain. As the result, 12.3% of those isolates were active against Staphylococcus aureus KCTC 1916, 7.6% were Staphylococcus aureus KCTC 1928, 3.9% were Eschericia coli KCTC 1924, 3.0% were Canida albicans KCTC 1940, and 2.2% were Salmonella typhimurium KCTC 1926. About 40% of those isolates showed antimicrobial activities at both two media but the others showed at either one. According to the genus of isolated strains, Streptomyces and Micromonospora showed activities with higher frequencies than others.

저체중 또는 과체중 청소년에서 캠프 프로그램을 이용한 영양 교육효과에 관한 연구

성미경,승정자,류화춘,박재년,박동연,최미경,조경옥,최선혜,이윤신,김유경,이은주 대한지역사회영양학회 2003 대한지역사회영양학회지 Vol.8 No.4

This study was performed to evaluate the effectiveness of a nutrition education program developed for nutritionally imbalanced adolescents. A summer nutrition camp was held for 23 overweight and 16 underweight subjects. Its effectiveness with regard to was evaluated at the end of the camp and 6 months later. Nutrition knowledge, nutritional attitude, food behavior, nutrient intake, exercise habit and ideal body figures desired by the subjects. The results showed significantly higher nutrition knowledge scores at the end of the camp as compared to those obtained prior to the camp program, and these scores were maintained for at least six months. Nutrition attitude scores also improved after the education program, and these improved scores also lasted for 6 months. However, the food behavior scores measured 6 months after the education program were not significantly different from those obtained prior to the camp. Also, the exercise habit, the ideal body figures and the body figures desired by the subjects remained unchanged. When nutrient intakes of subjects were assessed before the program and 6 months later, the mean daily vitamin C intake was significantly increased after the education program. Also, the intake of iron from plant food sources increased in the overweight subjects, while less iron from animal source were consumed by the underweight subjects. Both groups tended to consume more vegetables and fruits 6 months after the education program which may have contribute to the higher vitamin C and plant-based iron intakes. These results indicate that a 4-day nutrition education camp program sustained changes in nutrition knowledge and nutrition attitude for 6 months. The increased intake of vegetables and fruits was also achieved through this education program. However, changes in dietary behavior in adolescents may require repeated education.

세립형 고강도 구조용 강재를 이용한 보-기둥재의 실험적 평가

김요숙,임성우,장인화 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2(구조계)

As the structure becomes higher and longer-spanned nowadays, the need for strong and reliable structural material becomes greater. In order to apply high strength steel to the building structure, adaptability of the current design codes to the design with high strength steel has to be reviewed. Beam-column tests subjected to the simple and the combined bending and axial compressive load were performed with variable slenderness ratio. The ultimate strengths were satisfied with both of ASD and LHFD codes regardless of whether characteristic of yield phenomenon is continuous yielding or not.

장간막의 염증성 근섬유모세포종 - 1예 보고 -

임성직,김교영,박재훈,김윤화,박용구,이주희,양문호 대한병리학회 1999 Journal of Pathology and Translational Medicine Vol.33 No.9