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류재천,최윤정,김연정,김형태,방형애,송윤선 한국환경독성학회 1999 환경독성보건학회지 Vol.14 No.1
Recently, several new methods for the detection of genetic damages in vitro and in vivo based on molecular biological techniques were introduced according to the rapid progress in toxicology combined with cellular and molecular biology. Among these methods, mouse lymphoma thymidine kanase (tk) gene forward mutation assay, single cell gel electrophoresis (comet assay) and transgenic animal and cell line model as a target gene of lac I (Big Blue) and lac Z (Muta Mouse) gene mutation are newly introduced based on molecular toxicological approaches. The mouse lymphoma tk^(+/-) gene assay (MOLY) using L5178Y tk+i- mouse lymphoma cell line is one of the mammalian forward mutation assays, and has many advantages and more sensitive than hprt assay. The target gene of MOLY is a heterozygous tk^(+/-) gene located in 11 chromosome, so it is able to detect the wide range of genetic changes like point mutation, deletion, rearrangement, and mitotic recombination within tk gene or deletion of entire chromosome 11. The comet assay is a rapid, simple, visual and sensitive technique for measuring and analysing DNA breakages in mammalian cells. Also, transgenic animal and cell line models, which have exogenous DNA incorporated into their genome, carry recoverable shuttle vector containing reporter genes to assess endogenous effects or alteration in specific genes related to disease process, are powerful tools to study the mechanism of mutation in vivo and in vitro, respectively. Also in vivo acridine orange supravital staining micronucleus assay by using mouse peripheral reticulocytes was introduced as an alternative of bone marrow micronucleus assay. In this respect, there was an International workshop on genotoxicity procedure (IWGTP) supported by OECD and EMS (Environmental Mutagen Society) at Washington D, C, in March 25-26, 1999. The objective of IWGTP is to harmonize the testing procedures internationally, and to extend to finalization of OECD guideline, and to the agreement of new guidelines under the International Conference of Harmonization (ICH) for these methods mentioned above. Therefore, we introduce and review the principle, detailed procedure, and application of MOLY, comet assay, transgenic mutagenesis assay and supravital staining micronucleus assay.
형광검출 역상 액체크로마토그래피에 의한 Histamine의 정량
유희춘,김형룡,김상현,김대기,이영미,김형민,안년형,신태용 우석대학교 의약품개발연구소 1996 藥學硏究誌 Vol.1 No.-
Histamine을 정확하고 신속하게 정량하기 위해 9.Fiuorenylmethyl chloroformate를 형광유도체화제로 하여 역상 HPLC법으로 정량하였다. 히스타민을 형광유도체화할 때 반응액의 pH, 반응시간, 형광유도체화제의 농도 등 최적 반응 조건을 검토하였다. 이 방법으로 히스타민을 분석한 결과 0.1~0.5ug/ml의 농도범위에서 상관계수가 0.922인 양호한 직선성을 나타내었으며 검출한계는 0.01??/ml였다. A rapid and simple method for the determination of histamine by reverse-phase high performance liquid chromatography with fluorescence detection was established. 9-Fluorenylmethyl chloroformate(FMOC) was used as fluorescent derivative reagent. The optimum conditions for the derivatiation such as pH, reaction time and he concentration of FMOC were investigated. Linearity of calibration curve was obtained between 0.1ug/ml/and 0.5ug/ml(r=0.922) and the limit of detection was 0.01ug/ml.
가시층 분석 기법을 이용한 경관고도규제계획에 관한 연구 : 진주시 역사경관 보존을 중심으로
이춘석,강호철,류남형 진주산업대학교 1999 산업과학기술연구소보 Vol.- No.6
Chinju is famous for an old castle located in downtown. To conserve its historic landscape, some districts on the north of the castle have been under the height regulation. The historic landscape, however, is being seriously disturbed by the huge buildings newly constructed at the south of the castle. So, and alternative height regulation is required to make up its deficiency. This study examined the ‘View Floor Analysis(VFA)’as a height regulation planning tool, and suggested an alternative ‘Building Height Regulation Plan’with its results. The VFA is a new method to find out the maximum building height permissible from the differences between the existing topography and an imaginary floor which repersents building height theresholds, The process of the VFA used in this study was ① digitizing contours, ② converting digital contours to DEM, ③ manipulating control lines(super-imposing & text editing), ④ making visual floor(TIN) and converting it to raster data, and ⑤ map calculating(map algebra). The results were as follows: 1.At most, three stories(9m) buildings were permissilbe in the districts by the rear-gate of the castle, 2.But, very tall buildings over even twenty stories were allowable at Deulmal, Okbong, and the districts near the station. 3.The closer to the castle, the shorter building permissible. And, the closer to the mountains around the city, the taller building allowable.
보신 관광에서 감염된 수입성 Sparganosis 1예 : Intake of Thailand Snake during Trip
서환조,박명재,고경식,백영한,조유정,양현종,류경남,정형근,전정열 대한감염학회 1992 감염 Vol.24 No.1
Human sparganosois was reported more than 100 cases in Korean population. However no case reported yet in Korea as a patient was infected by sparganum from other geographical area, such as Thailand. The case we are reported here is a 38 year-old Korean male who had been suffered from mass being a small, round and soft consistance in upper portion of his right thigh. The patients stated that he had an opportunity to eat living snake in Bangkok, Thailand during trip. As therapy praziquantel (75㎎/㎏/day) was given p.o. for 14 days, but in vain to cure. Following medical treatment, surgical exicision was performed to remove a alive, soft, whitish-milky, mobile and elongated sparganum. Serlogical test by micro-ELISA for sparganum-specific antibody revealed a high serum antibody level of absorbance 0.67 (positive criterion: absorbance 0.22).
Endocrine Disrupting Activity of Seven Phthalate Analogues in vitro
Ryu, Jae-Chun,Kim, Hyung-Tae,Kim, Youn-Jung,Jeon, Hee-Kyung Korean Environmental Mutagen Society 2002 한국환경성돌연변이·발암원학회지 Vol.22 No.4
Phthalate analogues are a plasticizer and solvent used in industry. Phthalates were reported to be a potential carcinogen classified in the category of suspected endocrine disruptors. Most common human exposure to these compounds may occur with contaminated food. They may migrate into food from plastic wrap or may enter food from general environmental contamination. Since these substances are not limited to the original products, and enter the environment, they have become widespread environmental pollutants, thus leading to a variety of phthalates that possibly threaten the public health. Concern about their use has been mounting. To screen and elucidate the endocrine disrupting activity and their mechanism of phthalate analogues, first of all, E-screen assay was performed in MCF7 human breast cancer cells with seven phthalate analogues. In this cell proliferation assay, only dibutyl phthalate (DBP) showed weak estrogenic activity. Also the yeast-based transcription assay to assess the interactions of DBP with the estrogen, androgen, and progesterone receptors was conducted. DBP in the concentration ranges from 10$^{-16}$ to 10$^{-11}$ M was active in the estrogen transcriptional assay, but it did not show the effect on $\beta$-galactosidase activity in the progesterone and androgen transcriptional assays. These data indicate that DBP shows estrogenic potential and can be classified as weak and/or suspected endocrine disrupting chemicals.
운동과 카페인 투여가 에너지 기질 및 글리코겐 함량에 미치는 영향
류승필,장응찬,김형렬,이수천 한국운동영양학회 1998 Physical Activity and Nutrition (Phys Act Nutr) Vol.2 No.1
Caffeine has been used as an ergogenic aid to most of endurance athletes. As this point of view, we hypothesized that caffeine and training exercise could affect the glycogen contents in skeletal muscle and serum energy substrates in rats. Forty-eight male sprague-dawley rats had taken treadmill exercise at 28m/min/30min for 5days of week during 4wks. At the experimental day, all rats, except sedentary group(sed, n=12), were ingested 50㎎/㎏ of caffeine using round-ended needle directly into the mouth. They were killed at pre(pre-exercise group), 1hr(1hour exercise group), and 2hr(2hours exercise group) exercise and were followed by sed rats. Serum glucose, TG, FFA, and glycerol concentrations were analyzed and liver, soleus, gastrocnemius white muscle glycogen contents were determined. Serum FFA and glycerol were increased but glucose was decreased following exercise. Liver and muscle glycogen were not changed. In conclusion, even though serum FFA and glycerol increased, glycogen-sparing effect was not occurred. And, we thought that further research and discuss will be needed.
Ryu, BoMi,Choi, Il-Whan,Qian, Zhong-Ji,Heo, Soo-Jin,Kang, Do-Hyung,Oh, Chulhong,Jeon, You-Jin,Jang, Chul Ho,Park, Won Sun,Kang, Kyong-Hwa,Je, Jae-Young,Kim, Se-Kwon,Kim, Young-Mog,Ko, Seok-Chun,Kim, G The Korean Society of Phycology 2014 ALGAE Vol.29 No.4
Despite the extensive literature on marine algae over the past few decades, a paucity of published research and studies exists on red algae. The purpose of this study was to evaluate the potential therapeutic properties of the ethanol extract of the red alga Callophyllis japonica against lipopolysaccharide (LPS)-stimulated macrophage inflammation. The C. japonica extract (CJE) significantly inhibited the nitric oxide (NO) production and the induced dose-dependent reduction of the protein and mRNA levels of inducible nitric oxide synthase and cyclooxygenase-2. Additionally, the CJE reduced the mRNA levels of inflammatory cytokines, including tumor necrosis factor-${\alpha}$, interleukin (IL)-$1{\beta}$, and IL-6. We investigated the mechanism by which the CJE inhibits NO by examining the level of mitogen-activated protein kinases (MAPKs) activation, which is an inflammation-induced signaling pathway in macrophages. The CJE significantly suppressed the LPS-induced phosphorylation of c-Jun N-terminal kinase, extracellular signal-regulated kinase and p38 MAPK. Taken together, the results of this study demonstrate that the CJE inhibits LPS-induced inflammation by blocking the MAPK pathway in macrophages.