Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술발표논문집 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

Group Size And Measure Of Group-Level Properties

Kim,Kyoung-Su,Kim In-Sook,Jun,Gyung-Ju,Yang,Dong-Min 한국인적자원관리학회 2009 인적자원관리연구 Vol.16 No.4

Bliese와 Halverson(1998)은 집단수준의 효과를 분석하는데 있어서 기존에 사용되어 온 eta-squared 보다는 eta-squared 값이 사용되어야만 한다는 것을 주장하였다 그러나 이들이 제시한 수정된 eta-squared 접근은 다음과 같은 한계점을 가지게 되는 바 첫째, 이 접근법은 개인수준의 데이터를 집단 수준의 데이터로 만드는데 있어서의 정당성을 제공하는 기준을 구체적으로 제시하고 있지 못하다는 것을 들 수 있고, 둘째, 이 접근법은 또한 집단 내 효과를 용인하고 분석하는 기준을 제공하고 있지 못하다는 점을 들 수 있다 본 논문에서는 이러한 한계점을 극복할 수 있는 몇 가지의 대안을 제시하고 있으며, 이와 더불어 개인수준의 데이터를 집단 수준으로 만드는 것에 대한 정당성을 위한 또 하나의 추가적인 절차를 제시하였다 Bliese and Halverson(1998) argue that corrected eta-squared, rather than eta-squared, should be used for analyzing group-level effects However, their proposed corrected eta-squared approach has the following limitations (1) it fails to specify a criterion for justifying the aggregation of individual-level data to the group level and (2) it fails to specify a criterion for analyzing and allowing for within-group effects In this paper, we suggest several alternatives that overcome these limitations We suggest an additional procedure for justifying the aggregation of individual-level data to the group level

광주지역 소아에서 유행한 호흡기바이러스 유행양상 분석

서진종,김민지,김선희,기혜영,정재근,김은선,박종태,김경심,이수야,김명권,정윤석 대한감염학회 2008 감염과 화학요법 Vol.40 No.4

Background : This investigation was to perform the epidemiological surveillance and genetic analysis on respiratory viral agents from children with acute respiratory infections in Gwangju. Materials and Methods : For this study, 3,695 specimens obtained from patients with acute respiratory infections were collected by collaboration with pediatric hospitals in Gwangju between 2005 and 2007. Specimens were screened for 8 respiratory viruses including influenza viruses (IFV), human rhinoviruses (HRV), human coronaviruses (HCoV), adenoviruses (ADV), parainfluenza viruses (PIV), human enteroviruses (HEV), respiratory synthitial viruses (RSV) and human bocaviruses (HBoV). Respiratory viruses were detected using multiplex (RT) PCR with viral specific primers. Results : Out of 3,695 specimens, the ratio of virus detection was 24.9% (919). Overall, HRV (35.5%) and IFV (34.9%) were the most common viruses found, followed by HBoV (14.8%), HCoV (10.6%), RSV (3.7%), ADV (3.4%), PIV (3.2%) and HEV (3.0%). In addition, multiple infections were detected in 80 patients (8.7%). When the prevalence was analyzed according to season, HBoV, IFV and HCoV showed two epidemic points in late spring and early winter. ADV, HRV, RSV PIV and HEV, however, were all found to have only one epidemic point, with RSV being most common during winter and the others being most prominent during spring. Conclusions : Through this epidemiological surveillance, the respiratory viruses prevalent in children in Gwanaju area were investigated. We strongly recommend the development of nationwide policy for the management of prevalent respiratory virus that includes long term collection of data and samples, vaccine development and prevention education of the misuse of antibiotics.

남자 대학생의 음주 형태 변화에 영향을 미치는 유전적, 심리사회적 요인

김시경,이수경,김민경,이상익 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.4

Objectives : This study aimed to determine the general predictive factors of change in drinking behavior and to provide materials for preventing drinking problems during early adulthood through examining genetic and psychosocial factors affecting the change of drinldng behavior in college students. Methods : The subjects were 101 male college students, apart of 534 students who had completed the previous study in 2000. In the present study as a 6-years follow up, we reassessed the drinking pattem and psychosocial variables and compared the results with previous data of the same subjects. To identify factors affecting the current drinking pattem, we used stepwise multiple regession and logistic regression analysis. Results : D allele (ALDH2) was found to reduce the degree of drinking and suppress problematic drinking, and C allele (TPH) had a suppression effect on problematic drinking. Drinking motive had a direct effect on the degree of drinking and Pro-blematic drinking. Negative cognitive expectancy had a direct effect on problematic drinking. Conelusion : Authors found some factors affecting the change of alcohol drinking behavior in college students and confirmed that there were hierarchies of significance among these factors. These may be applicable as variables for predicting drinking behavior in early adulthood.

뇌졸중 환자의 기능 회복을 위한 치료적 운동 적용

김수경,이재신 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.14 No.6

This study is to review the application of therapeutic exercise for functional recovery in stroke patients. Peoples with stroke increase in korea and a almost all the stroke patients requires to serve early rehabilitation treatment program after onset. Stroke is syndrome characterized by inability to performance of functional activities include activities of daily living, work and leisure due to hemiplegic deficit of sensory and motor function, impaired cognition and perception, aphasia, apaxia, dysaphgia and psychosocial problem. Main treatment program is therapeutic exercise for sensorimotor function. Traditional sensorimotor approach are PNF(proprioceptive neuromuscular facilitation), Bobath's NDT (Neurodevelopmental treatment), Brunnstrom's movement therapy and Rood approach. The goal of therapeutic exercise program with stroke is functional recovery and improvement of life quality.

경력개발활동 유형이 성과에 미치는 영향

김경수,최광신,김봉광 全南大學校 企業經營硏究所 1997 産業經濟硏究 Vol.21 No.1

초록 본 연구의 목적은 조직 뿐만 아니라 개인 종업원들이 경력개발에 대한 책임을 느끼고 노력 을 하는 것이 그렇지 않을 때보다 효과적인지를 검증하는데 있다. 이를 위하여 길레이 (Gilley)가 주장한 경력개발활동을 기초로 개인개발활동과 조직의 경력개발활동의 시행여부, 활용정도, 파급효과 등에 대해 종업원이 지각하는 형태에 따라 경력개발유형을 부진 미흡형, 개별 분리형, 상호 보완형으로 분류하였으며, 이들 유형이 조직몰입과 이직의도, 주관적인 성과 등에 달리 영향을 미칠 것으로 보았다. 즉, 개인의 경력개발활동과 조직의 지원활동이 활발한 상호보완형의 경우 조직몰입도나 성과가 높게 나타날 것이고, 이직의도는 낮게 나타날 것이며, 이와 반대로 개인의 경력개발활동도 부진하고 조직의 지원활동도 미흡한 부진미 흡형의 경우 조직몰입과 성과는 낮게 나타날 것이고, 이직의도는 높게 나타날 것이란 가설 을 설정하였다. 12개의 금융회사의 직원들로부터 수집한 자료를 분석한 결과 경력개발활동 유형중 상호보완형에 속한 직원들은 높은 조직몰입과 주관적인 성과를 보였으며, 이직의도 는 낮은 것으로 나타났다. 반대로 부진미흡형에 속한 직원들은 낮은 조직몰입과 주관적인 성과를 보였으며, 높은 이직의도를 가지는 것으로 나타났다. 또한 개별분리형에 속한 직원들 은 중간 정도의 조직몰입과 성과를 보였으며, 이직의도 역시 중간 정도로 나타났다. 이러한 결과 결론부분에서 개인과 조직목표의 조화측면에서 논의되었다. <Abstract> The purpose of this study is to investigate the effects of Career Development Activites(CDA), which improves QWL and expedites the attainment of organizatonal goal, on performance. To accomplish this purpose, we classified CDA types into three dimentions(stagnation type, separation type, integration type) according to the employees perception regarding personal and organizational activities and analyzed the effects of CDA on performance such as organizational commitment, subjective outcome, and turnover intention. The results from 12 financial company showed that1) with regard to the level of organizational commitment and subjective outcome, employees in the separation type among CDA significantly and lowly differd from those in the stagnation type, 2) as for the level of turnover intention, employees in the separation type significantly and highly differed from thise in the integration type and significantly and lowly differed from those in the stagnation type. These results were discussed in terms of the fit between person and organizational goals.

수침목재의 동결건조 실험보고 : 대전 월평동 출토 유물을 중심으로

김경수,이용희 국립중앙박물관 1999 박물관보존과학 Vol.1 No.-

수침목재의 凍結乾燥 처리시 최상의 결과를 얻기 위해서는 대상유물의 상태와 ᅳ致할 수 있는 처리약품의 선정, 적용농도, 含浸방법 등 최적의 조건을 찾아내는 것이 필요하다. 이러한 조건설정은 전처리실험을 통해서만 가능하다. 이번 실험은 대전 월평동 유적에서 출토된 소나무(Pinus densiflora S. et Z.)와 상수리나무(Quercus acutissima Carruth.) 2種의 수침목재를 대상으로 2단계 PEG처리법(2-Step; PEG#200→PEG#4000), Sorbitol처리법, PEG#200+PEG#4000처리법,Sorbitol+PEG#4000처리법 등 4가지 방법으로 전처리한 후 동결건조하고 値數安定化效果를 비교하였다. 실험결과 2-단계 PEG처리법은 소나무材의 경우 PEG#4000을 60% 농도로, 상수리나무材의 경우 PEG#4000을 40% 농도로 처리한 것이 치수안정화효과가 가장 좋았다. Sorbitol 만을 적용한 처리법은 40% 농도로 처리한 소나무재에서만 비교적 양호한 결과를 얻을 수 있었다. 그리고 Sorbitol, PEG#200+PEG#4000, Sorbitol+PEG#4000으로 처리한 상수리나무는 기대한 만큼의 치수안정화효과가 나타나지 않았다. To get the best result from vacuum freeze drying of water-logged wood, it is necessary for objects to find out the best conditions such as chemicals, appropriate concentration of solution, impregnation method and etc. Such best condition could be set up by pre-treatment experiments. Two kinds of wood(Pinus densiflora S. et Z. and Quercus acutissima Carruth) were pre-treated by four methods; 2-step PEG treatment(PEG #200-PEG #4000), sorbitol treatment, PEG #200+PEG#4000 treatment, and sorbitol+PEG#4000 treatment. After those pre-treatment, vacuum freeze-drying was undertaken. Then the effect of dimensional stability were compared. When using 2-step PEG treatment, a solution of 60% PEG#4000 got the best dimensional stability for pine and in case of the oak, a solution of 40% PEG#4000 got the best. Sorbitol treatment got rather good result for the pine only when applied with 40% solution of sorbitol. Sorbitol, PEG#200+PEG#4000 and sorbitol+PEG #4000 treatments to the oak didn't affect on dimensional stability sufficiently.

건축공사시방서에서의 KS규격의 국제인증방안에 관한 연구

김경식,이재섭,김용수,이규진,조용훈,최완철,전재열,이현수,구재동,이기환 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1

The purpose of this research is make out construction specifications for International Construction. Using off-shore products incur cost increase and longer delivery term of construction. and inconvenient on International Construction. Expand the range of additional local products conforming to performance. Life Cycle Cost. maintainability. operability. user's acceptability, etc.. And this study is suggest the standard formats and the techniques for construction specification.

자가이식을 이용한 상악 매복 견치의 치료 : OF IMPACTED MAXILLARY CANINES

김수경,백병주,김재곤,양연미 대한소아치과학회 2007 大韓小兒齒科學會誌 Vol.34 No.3

뇌성마비 아동의 운동 기능 발달을 위한 재활 치료

김수경 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.6

This study is to review the rehabilitation treatment For motor Function development of children with cerebral palsy. Cerebral palsy(CP) is characterized by nonprogressive abnormality in the developing brain that create a cascade of neurologic, motor, and postural deficits in the developing child. Although a pattern of motor and postural deficits is a defining Feature of CP, many secondary disorders typically coexist with this diagnosis. Cognitive, sensory, and psychosocial deficits often Compound motor impairments and subsequent functioning. Main principle of treatment for motor function development is sensorimotor approach Traditional sensorimotor approach are Bobath's NDT(Neurodevelopmental treatment), Vojta approach and Rood approach. The goal of rehabilitation program with CP is functional capacity enhance and improvement of life quality.