Mechanism of the natural product moracin-O derived MO-460 and its targeting protein hnRNPA2B1 on HIF-1α inhibition

Soung, Nak-Kyun,Kim, Hye-Min,Asami, Yukihiro,Kim, Dong Hyun,Cho, Yangrae,Naik, Ravi,Jang, Yerin,Jang, Kusic,Han, Ho Jin,Ganipisetti, Srinivas Rao,Cha-Molstad, Hyunjoo,Hwang, Joonsung,Lee, Kyung Ho,Ko, Nature Publishing Group UK 2019 Experimental and molecular medicine Vol.51 No.2

<▼1><P>Hypoxia-inducible factor-1α (HIF-1α) mediates tumor cell adaptation to hypoxic conditions and is a potentially important anticancer therapeutic target. We previously developed a method for synthesizing a benzofuran-based natural product, (R)-(-)-moracin-O, and obtained a novel potent analog, MO-460 that suppresses the accumulation of HIF-1α in Hep3B cells. However, the molecular target and underlying mechanism of action of MO-460 remained unclear. In the current study, we identified heterogeneous nuclear ribonucleoprotein A2B1 (hnRNPA2B1) as a molecular target of MO-460. MO-460 inhibits the initiation of HIF-1α translation by binding to the C-terminal glycine-rich domain of hnRNPA2B1 and inhibiting its subsequent binding to the 3’-untranslated region of <I>HIF-1α</I> mRNA. Moreover, MO-460 suppresses HIF-1α protein synthesis under hypoxic conditions and induces the accumulation of stress granules. The data provided here suggest that hnRNPA2B1 serves as a crucial molecular target in hypoxia-induced tumor survival and thus offer an avenue for the development of novel anticancer therapies.</P></▼1><▼2><P><B>Cancer: How a plant metabolite analog suppresses tumor growth</B></P><P>A synthetic analog of a chemical found in fruit suppresses tumor growth by targeting an RNA-binding protein (hnRNPA2B1) and preventing the production of a pro-cancer regulatory factor. Nak-Kyun Soung from the Korea Research Institute of Bioscience and Biotechnology, Cheongju, South Korea, and coworkers built on their previous discovery that a compound derived from a medicinal plant metabolite can suppress the activity of hypoxia-inducible factor-1α (HIF-1α). This protein, which is involved in many aspects of cancer biology, is activated in the low-oxygen microenvironments found inside tumors. The researchers show that the compound binds to a protein that helps with the conversion of HIF-1α–encoding RNA transcripts into HIF-1α proteins. Liver cancer cells treated with the compound grew slowly and produced less HIF-1α under both normal and low-oxygen culture conditions, highlighting the potential of this anti-cancer strategy.</P></▼2>

맥반석의 용출시험 및 중금속 흡착특성

김종부,신명국,성낙환,최문정,김경주,이동석 강원대학교 산업기술연구소 2000 産業技術硏究 Vol.20 No.A

This experiment was done to investigate the leaching and adsorption properties of heavy metals on porphyry. The comparison with respect to the leachability of heavy metals from porphyry between the Korea Standard Leaching Test (KSLT) and the Toxicity Characteristic Leaching Procedure (TCLP) was carried out. The fractional composition of leachate and the total concentration of heavy metal of porphyry were studied through Sequential Extraction Procedure (SEP) and EPA Method 3050. Adsorption experiment of porphyry has pointed out that the optimum dosage of porphyry for 50ppm Pb was over 10g/L, the effective particle size for absorption was below 200 mesh and the optimum pH was about 7. From the Freundlich' adsorption equation, l/n was 1.0722, and k was 0.0041. After adsorption, the fractional composition of Pb was changed. The exchangeable, carbonate, reducible fractions were increased, and the organic fraction was not changed, and the residual fraction was decreased.

축냉재 포접화합물의 열물성에 관한 실험적 연구(Ⅱ) : 충전량 변화 Variations of Charging Conditions

김진흥,정낙규,고강옥,임재경,하옥남 朝鮮大學校 機械技術硏究所 1999 機械技術硏究 Vol.2 No.2

본 연구에서는 에너지의 합리적 이용을 위한 건물공조용 축냉시스템에 적용 가능한 축냉재로서 TMA-물계 포접화합물의 농도와 충전량의 변화에 따른 상변화온도, 비열 및 과냉도 실험을 통하여 다음과 같은 결론을 얻었다. 1. 충전량이 많을수록 전 실헐과정에서 열물성의 안정성이 높게 나타났다. 2. TMA-물계 포접화합물의 비열은 순수한 물에 대하여 86% ∼ 96% 정도를 나타내었다. 위의 실험결과로부터 TMA-포접화합물은 건물공조용 축냉재로서의 열물성을 갖는다고 판단된다 The objective of this Paper is to investigate the thermal properties of TMA-clathrate compound applicable to cold storage system for residential air-conditioning in order to use energy reasonably. Especially, the test tube experiments are performed to compare and analyze the concentration of TMA-clathrate compound, the temperature of heat source, and the phase change temperature, specific heat and subcooling characteristics according to the variation of charge capacity. The result is summarized as follows: the phase change temperature appears higher as the charge capacity is larger. Thus, it appears that TMA-clathrate compound has thermal properties as the cold storage medium for residential air-conditioning.

축냉재 포접화합물의 열물성에 관한 실험적 연구(Ⅲ) : 충전량 변화 Variations of Charging Conditions

임재경,고광옥,김진흥,정낙규,하옥남 朝鮮大學校 機械技術硏究所 2000 機械技術硏究 Vol.3 No.1

The objective of this paper is to investigate the thermal properties of TMA-clathrate compound in order to applicate cold storage system to residential air-conditioning. Especially, the test tube experiments are performed by comparing and analyzing the concentration of TMA-clathrate compound, the temperature of heat source, and the phase change temperature, specific heat and subcooling characteristics on the point of the variation of charge capacity. The results are summarized as follows; 1) When the temperature of heat source is -15℃, the stability of the thermal properties is appeared high in the whole experiments 2) The effect of subcooling suppression is improved around 0.6℃ when the temperature of heat source is -15℃, while the teemperature of subcooling is about 2.2℃ when the temperature of heat source is -10℃. Especially, it did not almost present any subcooling in the 26wt% of the 5g and the 25wt%, 30wt% of the 10g. Thus, we concluded that TMA-clathrate compound has thermal properties as the cold storage medium for residential air-conditioning.

콜레라독소의 피하투여에 의한 생쥐의 림프절 및 비장내 B 및 T 림프구의 분포변화

이원규,박낙진,김종원,허영범,김정혜,안희경 대한해부학회 1995 Anatomy & Cell Biology Vol.28 No.2

축냉재 포접화합물의 열물성에 관한 실험적 연구 (IV) : 온도 변화 Changing of Temperature

고광옥,임재경,김창오,김진홍,정낙규,하옥남 조선대학교 에너지.자원신기술연구소 2000 에너지·자원신기술연구소 논문지 Vol.22 No.2

The objective of this paper is to investingate the thermal properties of TMA-clathrate applicable to cold storage system for building air-conditioning in order to use energy reasonably. Especially, the test tube experiments are performed by comparing and to analyzing the temperature of phase change, specific heat and subcooling characteristic according to the variation of concentrations and temperature of heat source in TMA clathrate. The result are summarized as follows; 1) temperature of phase change is dropped as the temperature of heat source is lower 2) specific heat of TMA-clathrate is about 24~95 % of pure-water 3) subcooling is smalled as the temperature of heat source is lower. Thus, it apears that TMA-clathrate has thermal properties as the cold storage medium for residential air-conditioning

폐굴껍질을 산성토양 적용시 토양의 화학적 특성변화에 관한 기초 연구

임진희,문종익,김성우,성낙창,이영형,윤태경 동아대학교 환경문제연구소 1999 硏究報告 Vol.22 No.2

Oyster shell has caused environmental problems in the coast. But it is possible to be used as an acidic soil amendment because it is the alkalic material of pH 9.0. In order to evaluate the utility of the crushed oyster shell as a soil amendment, acidic sandy loam soil was amended with it and then Chinese cabbages were cultivated in pots. The amount of the oyster shell was ⅰ)none, ⅱ)0.5kg/㎥, ⅲ)1.0kg/㎥, ⅳ)1.5kg/㎥, ⅴ)2.0kg/㎥ and ⅵ)2.3kg/㎥. The particle size of oyster shell consists of 34.88% of 40∼100mesh and 14.98% of larger than 100 mesh. It increased pH and the contents of available P₂O_(5) and SiO₂ and exchangeable Ca in used soil The application of oyster shell also increased the height and diameter of Chinese cabbages.

사후분해 시체에 대한 법의학적 접근

최영식,이상용,김유훈,조갑래,이봉우,양경무,정낙은,서중석,이한영,이원태,강현욱 大韓法醫學會 2000 대한법의학회지 Vol.24 No.2

Disposal of a homicide victim by dismemberment is rare, but individual cases are on record in most major medicolegal departments. Recognition of postmortem mutilation may be of importance in the interpretation of certain murders committed by sexual perverts and other mentally deranged individuals and sometimes performed for the sole reason of easier disposal of the body. Postmortem dismemberment is usually readily recognizable as such; The edges of the injuries are dry and lack evidence of bleeding. The joints may be disarticulated without fracture, or the use of an axe or saw may be evident from examination of bones. Parallel horizontal or oblique furrows in the bone surface are caused by skipping of the saw prior to establishing depth. Such patterns on the bone may assist in identifying the particular saw involved. So we report 25 dismembered corpses that autopsied in National Institute of Scientific Investigation. This paper can help in attempting to establish not only the first criminal investigation steps but also the medicolegal approach methods in unidentified and dismembered deaths.

부직포활성슬러지법에 의한 하수와 침출수처리에 관한 기초연구

정유진,고현웅,김경순,윤태경,성낙창 한국환경과학회 2003 한국환경과학회지 Vol.12 No.10

In this study, it was performed using submerged nonwoven bioreactor(SNBR) for removal of organic matter, nitrogen and phosphate under different aeration intervals(intermittent aeration). We applied the SNBR at the cheap nonwoven fiber module instead of the expensive membrane. The SNBR was mainly made up of an activated sludge reactor and a transverse flow nonwoven module. with an innovative configuration being in application between them. In case of sewage, the aeration conditions experimented consist of continuous aeration and 60min/60min, 120min/60min, 120min/120min of aeration/nonaeration time intervals, respectively. In case of landfill leachate, the intermittent aeration condition was 120min/120min at aeration/nonaeration. Consequently. a high COD removal rate (about 94%) was achieved in sewage and leachate. Although nutrient removal rate was relatively high without any additional chemicals.

표면 플라즈몬 공명 신호의 증폭을 위한 새로운 금 콜로이드 센서칩 제조와 특성 연구

임정혁,이경호,고광락,강신원,김재호 경북대학교 센서기술연구소 1998 센서技術學術大會論文集 Vol.9 No.1

New gold colloidal film sensor chips were employed to enhance the sensitivity of the Surface Plasmon Resonce (SPR). Au collodal solutions were synthesized, and Au colloidal films were fabricated by immobilization of colloid on vacuum deposited Au film through the coupling agent of 1,8-diaminooctane. Specific interaction of human IgG antibody to human antigen was monitored by in situ SPR measurement from both a smooth and a Au colloidal films. Au colloidal film sensor chips resulted in 27 % enhancements effect for the same reaction on SPR angle shifts compared to a smooth Au film.