김치에서 분리한 Lactobacillus sp. JC-7과 Lactobacillus acidophilus 88간의 Electrofusion 최적조건 설정

조영배,최현정,백형석,전홍기 부산대학교 유전공학연구소 1997 분자생물학 연구보 Vol.13 No.-

김치 발효 숙성기간을 연장하고 신선도를 오랫동안 유지 할 수 있는 김치발효 starter의 개발을 목적으로 최적숙성기에 있는 김치로부터 bacteriocin생성능이 없는 유산균을 분리하여 동정하였으며, bacteriocin생성능이 우수한 L.acidophilus 88을 융합시키기 위해 electrofusion에 대한 최적 조건을 검토하였다. 분리균주는 Lactobacillus속으로 동정되어 Lactobacillus sp. JC-7이라 명명하였다. Electrofusion에 의해 생성된 융합주를 식별하기 위해 streptomycin(2.5㎎/ml)에 내성을 나타내는 Lactobacillus sp. JC-7변이주와 kanamycin(600㎍/ml)에 내성을 나타내는 L.acidophilus 88변이주를 분리하였다. Electrofusion을 100V/㎝, 120msec(72ohms,1670 capacitance) 에서 수행했을 때 융합효율이 가장 양호하였으며 전기장의 세기와 시간이 중가할수록 융합 효율이 현저히 감소하였다. 2가 양이온은 농도가 중가할수록 대체적으로 융합효율을 감소시키는 경향을 나타내었으나 1mM MGCl_2에서는 대조군에 비해 융합효율이 약간 증가하였다. PEG매개에 의한 융합법의 융합효율을 비교한 결과, 융합효율은 chemical fusion<electrofusion<electrofusion+20% PEG순을 나타내었다. Evaluation of Optimum Conditions for the Electrofusion between Lactobacillus sp. JC-7 Isolated from Kimchi and Lactobacillus acidophilus 88. Young-Bae Jo, Hyun-Jung Choi, Hyung-Suk Baik and Hong-Ki Jun*. Department of Microbiology, Pusan National University, Pusan 609-735,Korea-A lactic acid bacterium was isolated from kimchi. The isolated strain was identified as the genus Lactobacillus thrugh its morphological characteristics and named as Lactobacillus sp. JC-7. The optimum conditions for the electrofusion between streptomycin(2.5㎎/ml)resistant mutant of Lactobacillus acidophilus 88 and kanamycin(600㎍/ml) resistant mutant Lactobacillus sp. JC-7 were evaluated. The highest number of fusants were obtained at a capacitance value of 120msec(1670㎌), a field strength of 100V/㎝,and a pulse controller setting of 72Ω. The potimum pH of elecroporation buffer was 7.5 and the concentration of divalent cation was 1mM MG^2+. Electrofusants were efficiently obtained by addition 20% polyethylene glycol to electroporation buffer. The yield of fusion was better than that of using polyethylene glycol mediated chemical induction.

토양환경지도 자료를 이용한 콩밭 토양의 화학성 비교

박지숙,이민진,정재원,김미혜,이서연,지윤미,한준호,김유학,공명석,한광현,노희명 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.2

It is important to interpret the soil chemical properties in order to effectively manage the farmland. This study was conducted to investigate the soil chemical properties of upland soybean field according to the soil series at 32 upland fields located in the parts of Gangwon-do. Soil sampling sites were selected by using the National Statistics DB and KSIS DB that is a soil environment information system provided by the Rural Development Administration. Soil samples were collected from 0~15 cm of top soil before seeding or transplanting, air-dried, passed through a 2 mm sieve, and analyzed for soil pH, EC, organic matter, available phosphorus (P2O5), exchangeable potassium, exchangeable calcium and exchangeable magnesium. The average chemical properties have exceeded the each nutrient optimum range level of RDA, except the organic matter in experimental soil 2015. And despite application of conventional fertilization, each soil series nutrient contents were change respectively. The results of this study suggest that conventional fertilization should be applied based on the analysis about the amount of accumulated nutrient contents in the soil.

건축교육과정(커리큘럼) 수요인식에 대한 비교 연구 : 미국 분석 자료를 토대로 한 한국의 사례를 중심으로 Based on Examining the Curricular Demand Study of the United States in Korea

이준석,이상현 대한건축학회 2004 대한건축학회논문집 Vol.20 No.4

Current reformation of architectural education curriculum in Korea is widely based on that of the United Sates’. But in the United Sates, the result of curricular demand study has updated the content of the current NAAB’s accreditation criteria. It calls for an urgent need of conducting comparable study finding demands in practice for Korea, to better assess the validity of wide adaptation of the accreditation criteria from the U. S. as a reformation standard of architectural education in Korea. For this study, a survey asking demands in practice was answered by 72 registered architects in Seoul area. The result showed generally similar demands compared to that of the U. S.’ But demands in Korea also revealed noticeable difference by showing vast demand for architectural design ability, and demand for better knowledge and ability in the major architectural subjects. This study theorizes the proper adaptation of the criteria from the U. S. in Korea and suggests directions of necessary future studies.

풍소음 저감용 특수 다중도체 방식의 3차원 전계해석

민석원,송기현,김용준 순천향대학교 부설 산업기술연구소 2000 순천향 산업기술연구소논문집 Vol.6 No.2

This paper investigates characteristics of spiral charge for charge simulation method to calculate electric fields of special conductor bundles with spiral rods in 765 kV transmission line. We finds the arranging spiral charge in central position of spiral rod give less potential calculation error than lower position of spiral rod. When a spiral rod is simulated as spiral charge, we also knows two spiral charge with four integral interval can simulate spiral rod best.

민간 경호·경비원들의 의식구조와 가치관에 관한 연구

박준석,최현식,정성숙,김진환,정석현 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.4

The aim of this study was to investigate Consciousness and Value on Private Bodyguard and Security. I started to search from 7. January. 2004 to 20. May. 2004. so totally I searched for 5month, As a result, I got 526 papers of questionnaire and dropped 26 papers of them because of insincere answers from responders, So I used 500 papers for the research that I am doing. The method of One-Way ANOVA was used to verify the mean ratio and LSD multiple range test was also used for the verification when there was a slight difference among the objected group, All data were analyzed by SPSS 11.0 producer. The results of this study are like the below: 1) The result In the case of ages showed that significant difference(p<.05) companies consciousness were Private Bodyguard and Security for structure of consciousness. 2) The result In the case of Educational Credentials showed that no significant difference self consciousness, welfare conscious ness, social consciousness, companies consciousness were Private Bodyguard and Security for structure of consciousness. 3) The result In the case of work duration showed that significant difference(p<.05) self consciousness were Private Bodyguard and Security for structure of consciousness. 4) The result in the case of monthly income showed that no significant difference self consciousness, welfare conscious ness, social consciousness, companies consciousness were Private Bodyguard and Security for structure of consciousness. 5) The result in the case of working time showed that significant difference(p<.05) self consciousness, social consciousness, companies consciousness were Private Bodyguard and Security for structure of consciousness.

주요 국산수종의 휨가공성 평가

정인석,이원희,장준복,배현미 한국목재공학회 2002 목재공학 Vol.30 No.2

본 연구는 고주파 가열에 의한 산벚나무, 소태나무, 서어나무, 굴참나무, 박달나무, 고로쇠나무, 피나무, 소나무, 리기다 소나무, 참오동나무의 휨가공성을 평가해 보기 위하여 실시하였다. 본 연구에서 휨가공을 위한 곡율반경은 휨틀의 곡율반경에 의해 4 ㎝, 6 ㎝, 10 ㎝으로 구분하고, 대철의 두께는 0.6 ㎜과 0.8 ㎜로 구분하였다. 본 연구결과, 소태나무, 서어나무, 박달나무 고로쇠나무는 모든 곡율반경에서 100% 성공율을 나타내었다. 산벚나무와 굴참나무의 경우 58%와 83%의 성공율을 각각 나타내었다. 피나무와 오동나무의 경우 총 29%와 8%로 매우 불량하게 나타났고, 곡율반경 4 ㎝에서는 모두 실패로 나타났다. 침엽수재인 소나무와 리기다 소나무는 각각 44%와 56%의 성공율을 나타내었다. 대철의 두께는 휨가공성의 쉽고 어려움보다는, 건조시 건조속도에 더 많은 영항을 주는 것으로 나타났다. 건조과정에서 대철두께가 얇을수록 휨가공부재의 건조 속도가 빠른 경향을 나타내었다. This study was carried out to evaluate bending property on principal domestic species such as sargent cherry(Prunus sargentii), bitter wood(Picrasma quassioides), horn beam(Carpinus laxflora), cork oak(Quercus variabilis), birch(Betula schmidtii), painted maple(Acer mono), basswood(Tilia amurensis), red pine(Pinus densiflora), pitch pine(Pinus rigida), royal pawtonia(Paulownia tomentosa) by microwave heating. In this study, radius of curvature(ROC) for bending process was classified by radius of curvature(ROC) of bending plate such as 4 ㎝, 6 ㎝, and 10 ㎝, and thickness of metal-strap(TMS) was 0.6 ㎜ and 0.8 ㎜. Bending process was successfully operated for 100 percent in bitter wood, horn beam, birch and painted maple. On the other hand, there was a success rate of 58 percent in sargent cherry and 83 percent in cork oak and 29 percent in hasswood and 8 percent in royal pawlonia which is the worst bending property. All specimens of basswood and royal pawlonia were broken at 4 ㎝ of ROC. Success rate of bending property was shown 44 percent in red pine and 56 percent in pitch pine. TMS has an effect on only drying speed in drying process than difficulty and facility of bending property. It was considered that the thinner TMS in drying process is the faster in drying speed of bent wood.

단기배양을 통한 말초혈액 CD34 양성세포의 체외증폭

박상준,김철희,배광봉,김현수,김종숙,윤환중,조덕연,김삼용 충남대학교 의과대학 지역사회의학연구소 1997 충남의대잡지 Vol.24 No.1

Background: It is suggested that clinical practice in the areas of bone marrow transplantation and gene therapy might rely on the ex vivo expansion of hematopoietic stem/progenitor cells. However, the condition for ex vivo expansion of hematopoietic progenitor cells is not well established. The authors pursued a series of experiments to define the proper conditions for the expansion of hematopoietic cells in the short-term liquid suspension culture of mobilized peripheral blood CD34+ cells. Methods: 1.0ml cultures were initiated with 9×10^3 PB CD34+ cells, which were isolated from PB mononuclear cells (MNCs) by high-gradient cell sorting, in 12 well plates with the various combinations of hematopoietic growth factors(HGF). The following recombinant human HGFs were used: stem cell factor(SCF) 100ng/ml, granulocyte colony-stimulating factor(G-CSF) 100ng/ml, GM-CSF(granulocyte, macrophage colony-stimulating factor) 100ng/ml, interleukin-1 beta(IL-1B) 1ng/ml, interleukin-3(IL-3) 20ng/ml, interleukin-6 (IL-6) 100ng/ml. At the end of culture, colony-forming cells were evaluated by semisolid clonogenic assay. Results: 1) Using the high-gradient magnetic sorting system, CD34^+ cells were isolated with a yield of 40 3% 2) In 7 day culture of PB CD34^+ cells(9×10^3 cells), nucleated cells expanded mean 10×10^3(range, 9 to 20×10^3) with the addition of SCF alone, 35×10^3(range, 10 to 60×10^3) with SCF plus G-CSF plus GM-CSF, and 130×10^3(range, 40 to 300×10^3) with the combination of SCF, G-CSF, IL-1, IL-3, IL-6, GM-CSF. In 14 day culture, nucleated cells expanded 10×10^3 to 1,860×10^3 with combination of human hematopoietic growth factors. 3) In 10 day culture without medium change of PB CD34^+ cells, CFU-GM numbers expanded 16. 5 fold(range, 7 to 59 fold) with the addition of SCF plus G-CSF plus Il-1 plus IL-3, 31.3 fold(range, 20.5 to 101.1 fold) with the combination of SCF, G-CSF, IL-1, IL-3, GM-CSF. In 14 day culture with or without medium change of PB CD34^+ cells was inferior to 10 day culture for CFU-GM expansion. 4) There was no significant difference for CFU-GM expansion between five growth factors(SCF,G-CSF,IL-1,IL-3,GM-CSF) and six growth factors(five growth factors plus IL-6). Conclusion: The authors could confirm that short-term suspension culture of peripheral blood CD34+ cells could expand hematopoietic progenitor cells. Ten-day culture with medium change of CD34+ cells with the addition of five growth factors, i.e. SCF, G-CSF, IL-1B, IL-3, and GM-CSF, might be the most efficient in this system.

외상성 간손상과 비장손상시 CT분류의 의의

정준영,이창현,김현정,도호석,이삼범,도병수 大韓應急醫學會 1997 대한응급의학회지 Vol.8 No.1

The Organ Injury Scaling Committee of the American Association for the Surgery of Trauma recently published a consensus classification of hepatic and splenic injuries. The hepatic and splenic injury scales, based on parenchymal laceration and intrahepatic hematoma, include grade 1 to 6 and grade 1 to 5 respectively, representing the least to most severe injury. Forty-eight patients with hepatic and splenic injury were evaluate by abdominal CT from January 1995 to May 1996 and we compared abdominal CT grading with operative grading. Four CT grades did not correlate with operative finding. CT grading showed a sensitivity of 80%, specificity 80% in regard to operative grading. We conclude that 1) CT is an accurate technique to determine the extent of hepatic and splenic injury. 2) CT grading of hepatic and splenic trauma has a high correlation with operative grading.

帶狀疱疹으로 入院한 患者 1例에 對한 症例報告

정현아,류현신,이준성,노석선 대전대학교 한방병원 2001 惠和醫學 Vol.10 No.1

From December 17th, 2001 to January 5th, 2002, the clinical study was carried out I case of patient with herpes zoster, who has been hospitalised in the Dept. of E.N.T medicine. College of Oriental Medicine, Taejon University, Taejon, Korea. The result were like below: 1. Herpes zoster virus is developed by depression of immune reaction reactivate on dorsal root ganglion. Specialy herpes zoster occured on lumbar is called Junoh-whadan in oriental medicine. 2. Herpes zoster' pathogenetic is clasifiedclasified JunGiNaSang(情志內傷), BiSilGunUn(脾失健運), QiHulUngChe(氣血凝滯). 3. Step of herpes zoster' symptom is erythema, papula, vesicle, pustule, scab. 4. Herpes zoster' pathogenetic was clasified JunGiNaSang(情志內傷) is used for Ong-Dam-Sa-Gan-Tang(龍膽瀉肝湯), incipient symptom was used venesection, UhBangJa(牛蒡子) dressing, exposure of Infra-red.

공여자의 종류에 따른 간이식 후 감염성 합병증의 비교 : Comparison between Orthotopic and Living Donor Transplantation

기현균,손준성,오원섭,백경란,이남용,김성주,조재원,이석구,송재훈 대한감염학회 2004 감염과 화학요법 Vol.36 No.3

목적 : 최근 간이식 수요가 증가함에 따라 이에 대한 하나의 대안으로 생체 부분 간이식(LDLT) 시행례가 증가하고 있다. 이에 뇌사자 사체 간이식(OLT)과의 감염 양상을 비교하기 위하여 본 연구를 시행하였다. 방법 : 1996년 5월부터 2003년 8월까지 삼성서울병원에서 간이식을 시행 받은 284명의 환자들을 대상으로 후향적으로 분석하였다. 결과 : 188명은 LDLT를, 96명은 OLT를 시행 받았다. 간이식 후 감염증은 총 191명에서 발생하였고(67.0%) OLT 환자군과 LDLT 환자군 사이에 감염증의 발생 빈도와 원인 병원체의 빈도는 유의한 차이를 보이지 않았다. 병원체 별로는 Enterococcus 균정(16.2%) S. aureus(15.1%), coagulase negative staphylococci (13.4%)의 순서였다. 감염증의 종류는 복강 내 감염이 총 83례로 가장 많았는데, LDLT에서 OLT에 비하여 유의하게 높게 발생하였다(34.0% vs 19.8%, P=0.012). 요로 감염증(11.5% vs 4.3%, P=0.022)과 침습성 아스페르길루스증(7.3% vs 1.6%, P=0.034)은 OLT을 시행한 경우에 많았다. 기타 감염증은 통계적으로 유의한 차이가 없었다. 결론 : 간이식 환자에서 공여자의 종류에 따른 감염의 발생빈도는 차이가 없었으나 감염증 중 복강 내 감염은 LDLT에서 더 많이 발생하였다. 장구균 감염, 특히 반코마이신 내성 장구균이 간이식 후 발생하는 감염증의 중요한 원인 병원체임을 확인하였다. Background : Living donor liver transplantation (LDLT) has been performed more frequently to circumvent the shortage of donor organs. However, infectious complications after LDLT compared with orthotopic liver transplantation (OLT) have not been well investigated to date. Methods : We have retrospectively reviewed the medical records of all consecutive cases of liver transplantation, which had been performed at the Samsung Medical Center, Seoul, Korea, from May 1996 to August 2003. Results : Of the 284 patients enrolled, 188 (66.2%) underwent LDLT. Overall incidence of infectious complications after liver transplantation was 67.0% (191/284). Incidence of infections was not different between OLT group (67.7%) and LDLT group (67.0%) (P=0.9). Bacterial infections were the most common infectious complications (146 cases) followed by viral infections (32 cases), fungal infections (26 cases), and tuberculosis (4 cases). Enterococcus spp. (16.2%) were the leading pathogens followed by Staphylococcus aureus (15.1 %) and coagulase-negative staphylococci (13.4%). However, the distribution of etiologic agents was not different between OLT and LDLT group. Intra-abdominal infections (83 cases) were the most common type of infectious complications, which were more frequent in LDLT group (34.0%) than in OLT group (19.8%) (P=O.Ol). Conclusion : LDLT resulted in intra-abdominal infections more frequently than OLT. Enterococcus spp., especially vancomycin-resistant enterococci, are emerging pathogens in patients with liver transplantation.