Purge & Trap-GC를 이용한 의약품 필름코팅 정제 중 잔류용제에 관한 연구

장준식,이명자,소유섭,문춘선,이주헌,박희라,김진숙,강경모,이선옥,방성연,유미자,유문균,금오성,이병욱 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-

의약품은 약물을 생체에 적풋하기 위하여 유효성분의 효과가 언제나 일정하게 확보되고 사응에 편리하도록 만들어지는 것이므로 유효썽분 이외에 약효에 영향을 주지 않는 성분이 첨가되는 경운가 많다. 이 때 사용되는 용매들은 제피의 광택 및 건쪼시간의 단축 등을 위하여 휘발점이 낮을 용매들이 주로 사용되어진다. 본 연구는 의약품 필름코팅정제 중 잔류용매 4종(chlorofonr benzen, trichloro ethylen, 1,4-dioxane)에 대한 변형된 pirge & trap-GC 장치를 이용한 동시분석방법을 개발하였으며, 각 표준품의 RSD 값은 chloroform 3.03%, benzen 3.17%, trichloroethylen 3.69% and 1,4-dioxane 3.41%였다. 또한 시중 유통중인 의약품 50종에 대하여 잔류웅매 양을 측정하였으며, 검출되는 잔류용매는 한 건도 없었다. This study nras carried out to develope the analytical method for the mixture of chlorefonn, benzen, trichloroethylen and 1,4-dioxane simultaneously and determine the remainingorgauic solvents in coating tablets by Purge & Trap-GC. The results were as follouFs ; 1. Chloroform, benzen, trio:tloroethylen and 1,4-dioxane separated by tenax #5 trap by HP-624GC column by terrlperature programming. The peaks were separated completely at retentiontime of 6.88min for chloroform, 8.21min for benzen, 10.38miu for trichloroethylen and 11.95minfor 1,4-dioxane. 2. Standard RSD were individually chloroform 3.03%, benzen 3.17%, trichloroethylen 3.69%and 1,4-diorane 3.41%. 3. 60 samples were not detrcted chloroform, benzen, trichloroethylen and 1,4-dioxane.

인공타액에서 수종 아말감의 부식시 용해성분 및 표면 부식 생성물에 관한 실험적 연구

이명종,조승주 大韓齒科保存學會 1994 Restorative Dentistry & Endodontics Vol.19 No.1

Al-Sn 합금의 마찰 특성에 미치는 Sn 형상의 영향

李定根,朱大憲,金文鉉,金明鎬 대한금속재료학회 2002 대한금속·재료학회지 Vol.40 No.10

Effects of Sn morphology on the friction and wear characteristics of Al-Sn alloys were investigated by using the tribology tester, optical microscope and scanning electron microscope. Three different types of Sn morphologies named elongated, small network and large network structure were obtained by cladding and heat treatment. Wear resistance of the specimen having large network structure exhibited better anti-friction characteristics compare to that of the elongated structure. Also specific wear rate and friction coefficient decreased markedly with change of Sn morphology from elongated to large network structure. Abrasive and adhesive wear occurred for the elongated structure, while oxidation wear was observed for the network structure.

중추신경계에 침범한 속립성 결핵 : 증례보고

이태환,김헌주,허철,한용표,홍순기,변진수,김명순,조미연,김윤미 대한신경외과학회 1996 Journal of Korean neurosurgical society Vol.25 No.11

in recent times, central nervous system(CNS) tuberculosis has been rare and the prevalence of the focal form, the tuberculoma, varies from 1 per 200 to 1 per 1000. CNS tuberculosis occurs as a result of hematogenous spread from a primary focus. mostly pulmonary tuberculosis. It can be diffuse exudative leptomeningitis or a localized tuberculoma ; the former is more common. We report a case of miliary cerebral tuberculosis in a 24-year-old female who had been having headaches for 4 months. Magnetic resonance imaging showed numerous small round Gadolinium-enhanced supratentorial and infratentorial lesions scattered throughout the brain. Histologic examination confirmed well defined tuberculous granulomas with central caseous necrosis in open biopsy, containing several acid-fast bacilli, the patient was treated with isoniazid, rifampin, ethambutol, pyrazinamide in combination with prednisolone.

GC와 GC-MS에 의한 어란의 지방산 분석

이주현,전명숙,이명환 서울여자대학교 자연과학연구소 1995 자연과학연구논문집 Vol.6 No.-

9종류의 어란에서 지방산을 추출하여 GC-MS로 분석한 결과 다음과 같은 결과를 얻었다. 1. 어란중 지방산을 GC와 GC-MS로 분석할 때 초기온도를 60°C로 하고 승온률을 9°C/min, 마지막 온도를 220°C로 하는 승온법이 목적 성분을 분리하는데 가장 좋은 결과를 얻었다. 2. 가자미, 날치, 대구, 명란, 농어, 성어, 오징어, 참치, 청어의 알에서 지방산을 추출하여 GC로 분석한 결과 포화 지방산은 myristic acid, palmitic acid, stearic acid, arachidic acid가 검출 되었고, 불포화 지방산은 oleic acid, linolenic acid 가 각각 확인 되었다. 3. 포화 지방산은 myristic acid가 명태알에 11.8㎍/g 으로 가장 많이 함유되어 있었고, palmitic acid는 오징어알에서 106.32㎍/g으로 가장 많이 함유되어 있었다. Stearic acid는 농어알에 53.03㎍/g, arachidic acid는 대구알에 8.5㎍/g으로 가장 많은 양이 함유되어 있었다. 4. 불포화 지방산인 oleic acid와 linolenic acid의 함량을 분석한 결과 oleic acid는 가자미 알에 linolenic acid는 명태알에 각각 109.12㎍/g, 62.48㎍/g으로 가장 많은 양이 함유되어 있었다. Fatty acids were extracted from the roe of flounder, flying fish, Pacific cod, Alaska Pollack, mero, shark, squid, tuna, and herring. They were methylated and analyzed by GC and GC-MS. Most of fatty acids found from 9 different roe were myristic acid, palmitic acid, stearic acid, arachidic acid, oleic acid, and linolenic acid. The roe of Alaska Pollack, squid, mero, and Pacific cod contained 11.8ug/g, 62.48 ug/g, of unsaturated fatty acids such as oleic acid and linolenic acid, respectively. The roe of flounder and shark had higher content unsaturated fatty acids than rest of the roe. The roe of Pacific cod, Alaska Pollack, mero, squid, tuna, and herring had larger amount of saturated fatty acids than the rest of the roe.

Immunoblot법을 이용한 Anisakis증의 혈청학적 진단

이영주,주경환,정명숙,임한종 고려대학교 의과대학 1990 고려대 의대 잡지 Vol.27 No.1

Acute gastric anisakiasis is rather easily diagnosed by endoscopy or radiological technique. But preoperational diagnosis of acute intestinal anisakiasis is very difficult. Furthermore, the diagnosis of chronic anisakiasis is more difficult because the worm invade the stomach or intestinal wall and finally to degenerate. Serodignosis may helpful in such diagnostic difficulties. However, any of the serodiagnostic method in anisakiasis has limitations for practical use due to cross reactions. In this context, the study was performed to observe the specifically reacting antigenic bands of crude extract Anisakis larval worm antigen and their reaction to the serum antibody obtained from experimentally immunized and infected rabbit, confirmed human anisakiasis and other helminthic infection cases using SDS-PAGE and EITB. The results obtained were as follows; 1. Among 12 cases of confirmed anisakiasis, eleven showed positive reactions by ELISA. The average 0. D. value of the test was 1.035. Two from 4 D. latum infected cases and one from 4 A. lumbricoides infected cases showed positive reaction respectively. 2. Silver stained SDS-PAGE of crude Anisakis larval worm antigen revealed more than 45 antigenic protein bands. Among them, 186, 76, 72, 65, 59~62, 54, 52, 50, 49, 46, 43, 40, 33, 32, 29, 24, 23, 18, 17, and 13Kd bands were major bands. Protein bands of 12Kd relative molecular weight was observed in A. suum extract antigen, on the other hand 29 and 46Kd bands were not observed. 3. Major antigenic bands of crude Anisakis worm antigen which reacted with serum antibody of confirmed anisakiasis cases were 186, 116, 72, 52, 46, 40, 33 and 32Kd band. Most of them also reacted with the sera of D. latum, A. lumbricoides, T. trichiura infected cases except 46Kd band which showed positive resuits only with sera of anisakiasis. So, this band could be considered as diagnostic band for anisakiasis especially for the identification of cross reactions against A. lumbricoides and D. lafum infection. 4. Although, low molecular weight bands such as 33 and 32Kd were observed as species specific, the sensitivity of that fractions were obviously low. Consequently, the diagnostic use of the low molecular weight protein bands were not promising. 5. Serum of rabbit immunized with Anisakis larval worm antigen also showed positive reaction on 46Kd bands, but that of A. suum and T. canis did not. These results were in accord with the results in human cases. In conclusion, 46Kd bands of crude Anisakis larval worm antigen can be considered as a diagnostic one for the identification of cross reactions using immunoblot technique.

현훈 환자 Neuron Specific Enolase의 임상적 의의

김원주,김선곤,유철형,김원찬,김용덕,최영철,이명식 대한신경과학회 1998 대한신경과학회지 Vol.16 No.4

외부온도에 따른 폴리머 콘크리트의 강도특성 변화

이윤수,주명기,최만용 강원대학교 부설 석재복합신소재 제품연구센터 2002 석재연 논문집 Vol.7 No.-

불포화 에스터 수지의 큰 이용분야의 하나로 건축재료가 있으나 여기서 가장 문제로 되어 있는 것이 바로 열적 특성의 개선이다. 따라서 본 연구에서는 외부온도가 폴리머 콘크리트의 강도특성에 미치는 영향을 검토하기 위하여 외부온도 20∼120℃ 범위로 하여 폴리머 콘크리트의 물리·역학적 특성을 구명하는데 목적이 있다. 본 연구에서는 불포화 폴리에스터 수지를 결합재로 하고 탄산칼슘을 충진재로, 규사를 잔골재로 치환하고, 굵은골재를 쇄석으로하는 폴리머 콘크리트를 제조하여 외부온도에 따른 강도특성을 검토한 바, 폴리머 콘크리트의 사용 적정온도는 50℃이내가 적당할 것으로 판단되며, 50℃이상에서 사용할 경우는 난연성이 우수한 탄소섬유를 사용해야 할 것이다. In this study, the polymer concrete was made to investigate its strength properties, in which unsaturated polyester resin was used as s binder, calcium carbonate as the filler and silica sand as th fine aggregate, and crushed stone as the coarse aggregate. The effects according to the external temperature on strength properties of polymer concrete were examined. Test result showed that the compressive, flexural and tensile strengths of the polymer concretes decreased with increasing external temperature. The elasticity of modulus of the polymer concretes decreased with increasing external temperature.

높은 휨강도를 갖는 시멘트 모르타르 및 콘크리트의 개발

이윤수,주명기 강원대학교 부설 석재복합신소재 제품연구센터 2003 석재연 논문집 Vol.8 No.-

본 연구에서는 강도 및 내구성이 우수한 프리캐스트 판넬을 개발할 목적으로 폴리머 및 단섬유 혼입한 시벤트 모르타르를 제조하여 시벤트 모르타르의 물리ㆍ역학적 성질에 미치는 폴리머 및 단섬유 혼입의 영향에 대하여 검토하였다. 그 결과로, 고강도 모르타르에서는 보동강도 모르타르에 비해 섬유의 부착강도가 크게 되기 때문에 아라미드의 고장도 섬유를 혼입함에 따라 휨강도는 매우 증가하였다. 또한 폴리머 및 단섬유 혼입에 의해서 고강도 모르타르의 취성이 개선되어 휨 변형에 대한 신장능력이 뚜렷하게 증가하였다. A high flexural strength results in a reduction of wall thicknesses of precast concrete products such as curtain walls and permanent formworks which assists craneage requirements or allows for larger panels. In the previous paper, flexural strength of 24MPa can be attained in water-cured cement mortars by reducing W/C, and using an optimum grading high strength sand. In this study, the effects of polymer-modification and discontinuous short fiber reinforcement on the properties of high strength mortars in the fresh and hardened states were assessed experimentally. The studied variables include polymer-cement ratio(0 to 10%), polymer types(SBR, PAE and epoxy), fiber types(vinylon and aramid), fiber volume fractions(0 to 3%), strength of matrix mortar(compressive strength of 42MPa and 112MPa), and curing conditions(water, wet and dry). The test data generated in this study indicated that improvements in flexural strength and toughness characteristics of air-cured high strength mortars can be achieved by PAE and epoxy polymers with dosage of 10% of the cement amount. It is found that use of high strength fibers such as aramid, not only reduces the brittle nature of high strength cementitious matrices, but also increases the maximum matrix strength.

경량 인조대리석의 강도 개선 및 몰드의 균열저감에 관한 연구

이윤수,주명기 강원대학교 부설 석재복합신소재 제품연구센터 2002 석재연 논문집 Vol.7 No.-

본 연구는 경량 인조석 제조공장의 애로사항 중 하나인 낮은 인조대리석의 강도와 몰드의 수축을 개선시켜 제조기술의 경쟁력을 높일 목적으로 수행된 것이다. 본 연구에서는 백색 시멘트 및 고강도 시멘트를 결합재로 하고 강모래를 잔골재로, 굵은 모래를 인공경량골재로 하는 경량 인조대리석을 제조하여 감수제 첨가량, 양생기간 및 경량골재 치환율에 따른 강도특성을 검토하였으며, 가소제 첨가량 및 EVA 첨가량에 따른 몰드의 건조수축을 검토하였다. 그 결과 경량 인조대리석의 휨 및 압축강도는 감수제 첨가량의 증가 및 양생기간의 경과에 따라 증가하였다. 또한 경량골재 치환율의 증가에 따라 강도는 감소하였다. 또한 몰드의 건조수축은 가소제 첨가량이 증가함에 따라 증가하였고, EVA 첨가량이 증가함에 따라서는 감소하는 경향을 보였다. In this study, an artificial lightweight marble was made to investigate the reduction of crack and improvement of strength, in which white cement and high-strength cement were used as a binder, river sand as the fine aggregate, and artificial lightweight aggregate as the coarse aggregate. The effect of shrinkage reducing agent content and of plasticizer content on drying shrinkage and the strength properties of artificial lightweight marble were examined. Test results show that the compressive and flexural strengths of the artificial lightweight marble increased with increasing shrinkage reducing agent content. The compressive strength of artificial lightweight marble decreased with increasing artificial lightweight aggregate content. The drying shrinkage of the mold increased with increasing plasticizer content, and decreases with increasing EVA content.