The Effect of Rosiglitazone on the Cell Proliferation and the Expressions of p27 and Skp2 in<i>Helicobacter pylori</i>Infected Human Gastric Epithelial Cells

Kim, Sung-Soo,Cho, Young-Seok,Kim, Hyung-Keun,Shin, Ok-Ran,Chae, Hiun-Suk,Choi, Myung-Gyu,Chung, In-Sik The Korean Society of Gastroenterology 2010 대한소화기학회지 Vol.55 No.4

<P>Background/Aims: Ligands for peroxisome proliferator-activated receptorgamma (PPARgamma), a member of the ligand-activated nuclear receptor superfamily, exhibit anti-tumoral effects and are associated with de novo synthesis of proteins involved in regulating the cell cycle and cell survival/death. Helicobacter pylori (H. pylori) is an etiologic agent for gastric adenocarcinoma, and raises the cell turnover of gastric epithelium. The aim of this study was to investigate the effect of PPARgamma ligand rosiglitazone on the cell proliferation and the expressions of p27 and Skp2 protein in H. pylori infected gastric epithelial cells. Methods: We examined the expression of PPARgamma by Western blot in H. pylori infected AGS human gastric epithelial cells. The effect of rosiglitazone on the survival of H. pylori infected AGS cells was assessed by cell viability assay. After the treatment of rosiglitazone in H. pylori infected AGS cells, the expressions of p27 and Skp2 were assessed by Western blot. Results: The expression of PPARgamma protein was increased in H. pylori infected AGS cells. Cell growth was inhibited and decreased in dose- and time- dependent manner in H. pylori infected AGS cells treated with rosiglitazone. A decrease in Skp2 expression and a reciprocal increase in p27 expression were found in dose- and time-dependent manner in H. pylori infected AGS cells treated with rosiglitazone. Conclusions: Rosiglitazone inhibited the growth of H. pylori infected AGS cells. Rosiglitazone attenuated Skp2 expression, thereby promoting p27 accumulation in H. pylori infected human gastric epithelial cells. Further studies will be needed to find the effects of accumulation on cell turnover in H. pylori infection and the role in the H. pylori-associated gastric carcinogenesis.</P>

Four Taeniasis saginata Cases Diagnosed at a University Hospital in Korea

Eun Jeong Won,신주현,이유정,김문주,강승지,Sook In Jung,Soo Hyun Kim,Jong Hee Shin,채종일,신성식 대한기생충학ㆍ열대의학회 2019 The Korean Journal of Parasitology Vol.57 No.3

In recent years, the taeniasis has been rarely reported in the Republic of Korea (Korea). But in this study, we in- tend to report 4 taeniasis cases caused by Taenia saginata during a 5-month period (February to June 2018) at a unversity hospital in Gwangju, Korea. Worm samples (proglottids) discharged from all cases were identified by phenotypic and mo- lecular diagnostics. Mitochondrial cytochrome c oxidase subunit I sequences showed 99.4-99.9% identity with T. sagina- ta but, differed by 4% from T. asiatica and by 7% from T. multiceps, respectively. We found that tapeworms in 2 cases (Cases 2 and 3) yielded exactly the same sequences between them, which differed from those in Cases 1 and 4, sug- gesting intra-species variation in tapeworms. These taeniasis cases by T. saginata infection in this study, which occurred within a limited time period and region, suggest the possibility of a mini-outbreak. This study highlights the need for fur- ther epidemiological investigation of potentially overlooked cases of T. saginata infection in Korea.

신원방우황청심원의 심혈관계에 관한 약효

조태순,이선미,김낙두,허인회,안형수,권광일,박석기,심상호,신대희,박대규 성균관대학교 약학연구소 1999 成均藥硏論文集 Vol.11 No.-

Abstract-In order to investigate the phamacological properties of New Wonbang Woohwangchungsimwon Pill (NSCH), effects of Wonbang Woohwangchungsimwon Pill (SCH) and NSCH were compared using various experimental models. In rat aorta, NSCH and SCH made the relaxation of blood vessels in maximum contractile response to phenylephrine (10^-6 M) regardless to endothelium containing or denuded rings of the rat aorta. Furthermore, the presence of the inhibitors of NO synthase and guanylate cyclase did not aftect significantly the relaxing effects of NSCH and SCH, NSCH and SCH inhibited the vascular contractions induced by acetylcholine, prostaglandin endoperoxide or peroxide in a dose-dependent manner. In conscious spontaneously hypertensive rats (SHRs). NSCH and SCH decreased significantly heart rate. These, at high doses, had a negative inotropic effect that was a decrease of left ventricular developed pressure and (-dp/dt)/(+dp/dt) in the isolated perfused rat hearts, and also decreased the contractile force and heart rate in the isolated rat right atria. In guinea-pig papillary muscle, these had no effects on parameters of action potential such as action potential amplitude (APA). V_max and resting membrane potential (RMP) at low doses, whereas inhibited the cardiac contractility at high doses. Furthermore, these had a significant inhibitory effects on palpitation of the heart in normotensive rats and SHRs. These results suggest that NSCH and SCH have weak cardiovascular effects, and that there is no significant differences between cardiovascular effects of two preparations.

접착제(본드)흡입이 간장의 기능 및 조직변화에 미치는 영향

김인수,손동렬,신인철 漢陽大學校 環境科學硏究所 1984 環境科學論文集 Vol.5 No.-

접착제의 흡입으로 일어날 수 있는 유해작용에 대하여는 이미 많은 연구가 이루어져 왔으나 그 유해작용은 접착제 제조에 사용된 유기용매의 종류와 순수성에 따라 좌우되므로 만성독성으로 인한 신경장애에 대한 작용이외에 많은 연구들이 일치된 견해가 없고 각 나라마다 자국산의 접착제에 대하여 철저한 연구가 이루어져 왔으나 우리나라에선 거의 연구되어 있지 않는 실정이다. 따라서, 우리나라에서 생산되는 접착제의 흡입으로 일어날 수 있는 만성독성에 대한 연구가 필요하여 백서에 장기간 접착제를 흡입시켰을 때 특히 간 기능과 조직변화에 미치는 영향을 연구하였다. 접착제는 사용된 유기용매의 종류에 따라 toluene과 acetone이 주 용매인 Gt와 hexane과 acetone이 주 용매인 Gh 2 종류로 구분하였으며 실험동물은 체중 180g 내외의 웅성 백서를 대조군, Gt 흡입군과 Gh 흡입군 3군으로 나누었다. 1) 접착제를 10분간 흡입시키는 동안에는 약간의 흥분작용 이외에 별다른 증상을 볼 수 없었으나 40일간의 흡입에서 부터 흥분작용이 증가되어 공격적이며 적의심을 나타냈다. 이러한 중추신경에 대한 영향은 Gt 흡입군에서 더욱 심화하였고 흡입이 중단된지 60일이 경과되어도 정상으로 회복되지 않았다. 2) 혈청 GOT와 GPT는 10일에서 부터 증가되어 30∼40일에 최고의 증가치를 나타냈으나 흡입이 계속되고 있음에도 감소되였다. 혈청 alkaline phosphatase 수준은 20일에서부터 증가하여 50 일까지 지속되었으나(59.2%∼73.1%) 60일에서는 감소되었다. 총 bilirubin은 전 실험기간에서 별 변동이 없었으며 혈청효소의 변동은 흡입이 중단된 후 20일에서는 거의 정상수준으로 회복되었고 Gt와 Gh 흡입군에서 유의한 차이가 없었다. 3) 간의 무게를 체중비로 나타낸 결과 20일에서 부터 25% 증가되었고 60일에서는 50%의 증가를 나타냈으나, 흡입이 중단된 후 50일이 경과되어서는 정상으로 회복되는듯 하였다.(Gt 12.2%, Gh 16.3%) 4) 간의 병리 조직학적 소견으로는 20일에서 문맥주변부의 염증 세포 침윤, 충혈 및 간세포 종창이 관찰되었으나, 흡입이 계속되고 있음에도 더 이상 약화되지 않고 60일간 같은 변화를 보였다. 흡입이 중단된 후 40 일에서는 정상 조직상태를 나타냈으며 이러한 조직변화는 Gt와 Gh 흡입군에서 별 차이가 없었다. 이상의 결과로 보아 우리나라에서 생산되는 접착제의 만성흡입은 내성의 출현으로 인하여 특정적인 심한 간 손상을 유발하지 않는 것으로 판단되며 간 기능 손상은 조직변화에 선행되어 나타나고 간 기능 및 조직손상은 회복성이 있는 것으로 사료된다. 우리나라에서 생산되는 접착제는 비교적 장기간 백서에 흡입하여 다음과 같은 결론을 얻었다. 접착제의 만성흡입은 특징적인 심한 간 손상을 유발하지 않았으며 이는 내성의 출현으로 인한 것으로 판단되고 간 기능 손상은 조직 변화에 선행되어 나타나고 간 기능 및 조직 손상은 회복성이 있는 것으로 사료된다. There have been a number of studies on the acute and chronic toxicities induced by continuing, heavy exposure to glue or its solvents. However the reports besides on the neuronal damage are still controversial, for the toxic actions probably depend on the kind and purity of the solvents used in glue. The aim of the present study was to evaluate the effect of chronic exposure to the inhalation of glue on the functional and histological liver damages. 210 healthy Sprague-Dawely male rats weighing about 180g. were divided into 3 groups: 1)control group exposed to the inhalation of fresh air, 2) Gt group exposed to the inhalation of glue whose main solvents were toluene and acetone, 3) Gh group exposed to the inhalation of glue whose main solvents were hexane and acetone. The results obtained were as follows: 1) During 10 minutes exposure to the inhalation of glue, slight excitement was observed in all rats and in 40 days exposure, excitement was more profound and aggressiveness and hostility were found in Gt group rather than Gh group. The excitement was not subsided even 60 days after stopping inhalation. 2) Serum GOT and GPT levels were elevated in 10 days exposure and reached to the highest level in 30∼40 days exposure followed by decrease to normal ranges. Serum alkaline phosphatase level remained raised from 20 days to 50 days exposure (59.2%∼73.1%) and returned to almost normal level in 60 days exposure. Total bilirubin level were within normal limits in all experimental period. The serum enzyme levels were recovered to normal ranges 20 days after being exposed to the final inhalation and have no significant difference between Gt and group. 3) The relative liver weights were increased by 25% in 20 days exposure, and by 50% in 60 days exposure but it seemed to be returned to normal weight 50 days after stopping inhalation (Gt 12.2%, Gh 16.3%). 4) The histopathological changes induced by 20 days exposure to the inhalation of glues Gt and Gh were mononuclear cell infiltration in periportal zone, congestion and cellular swelling on the liver and were not enhanced even by increase in exposure time. These findings were completely subsided 40 days after receiving the final exposure. From these results, it may be concluded that the chronic inhalation of glues available in Korea fails to cause severe or characteristic liver disease, the functional damage is followed by the histological changes in liver and the functional and histological damages are reversible.

Photoreflectance 측정에 의한 InxGa₁-xAs(0.03≤X≤0.11) 에피층의 특성 연구

김인수(In-Soo Kim),손정식(Jeoog-Sik Son),이철욱(Cheul-Wook Lee),배인호(In-Ho Bae),임재영(Jae-Youog Leem),한병국(Byung-Kuk Han),신영남(Young-Nam Shin) 한국진공학회(ASCT) 1998 Applied Science and Convergence Technology Vol.7 No.4

Molecular Beam Epitaxy(MBE)법으로 성장된 In_xGa_(1-x)As/GaAs 에피층에 대해 photoreflectance (PR)실험을 통해 특성을 조사하였다. PR 측정결과 성장된 In_xGa_(1-x)As 에피층의 띠간격 에너지(E。) 신호가 시료의 변형(strain)에 의해 heavy-hole(E。(HH))과 light-hole(E。(LH))로 분리되어 관측되었다. 에피층의 조성과 변형은 각각 시료에서의 E。(HH) 및 Eo(HH)와 Eo(LH)신호의 에너지 차이를 이용하여 구하였다. 또 160 K이하의 온도에서는 Eo(LH)의 신호가 사라짐을 볼 수 있었다. Franz-Keldysh oscillation(FKO) 피크로부터 계산되어진 InGaAs/GaAs 계면전장 (E)은 In 조성의 증가에 따라 0.75×10^5 V/㎝에서 2.66×10^5 V/㎝로 증가하였다. In 조성이 x=0.09인 시료에 대한 PR신호의 온도의존성 실험에서 Varshni 계수와 Bose-Einstein 계수들을 각각 구하였다. Photoreflectance (PR) measurents have been performed on In_xGa_(1-x)As/GaAs grown by molecular beam epitaxy (MBE). Bandgap (E。) of In_xGa_(1-x)As epilayer measured from PR was separated as heavy-hole (E。(HH)) and light-hole (E。(LH)) by strain effect. The compositions and the strains of epilayer were obtained from the energy value of E。(HH) and from enegy difference of E。(HH) and E。(LH), respectively. In addition, the PR signal of E。(LH) was diminished below 160 K. The interface electric field (E) of InGaAs/GaAs was increased from 0.75×10^5 V/㎝ to 2.66×10^5 V/㎝ as In composition increased, which was calculated from Franz-Keldysh oscillation (FKO) peaks. As the temperature dependence of the PR signal at x=0.09 sample, we obtained Varshni and Bose-Einstein coefficients.

Wolff-Parkinson-White증후군의 임상상 및 전기생리학적 소견

안신기,이문형,장양수,오동진,최인석,하종원,임세중,김병옥,정남식,심원흠,조승연,김성순 대한순환기학회 1997 Korean Circulation Journal Vol.27 No.9

토끼 수막염 모델을 이용한 다제내성 폐렴구균에 대한 Meropenem의 치료 효과

김신우,진정화,강수정,정숙인,김연숙,기현균,김춘관,이혁,김성민,백경란,송재훈 대한화학요법학회 2001 대한화학요법학회지 Vol.19 No.1

목적 : 다제내성 폐렴구균에 의한 수막염은 기존과 단일 항생제로는 근치가 어려워 ceftriaxone과 vancomycin 혹은 rifampin의 병용요법이 권유되고 있다. 다제내성 폐렴구균에 사용할 수 있는 다른 약제 중 meropenem은 시험관내 폐렴구균에 대한 항균력이 좋고 경련 등의 중추신경계 부작용이 거의 없어 다제내성 폐렴구균에 의한 수막염의 치료에 이용할 수 있을 것으로 기대되나 이에 대한 자료가 매우 부족한 상태이다. 이에 연자 등은 meropenem과 meropenem+vancomycin의 병용요법이 다제내성 폐렴구균 수막염의 치료에 효과가 있는지를 토끼 수막염 동물 모델을 이용하여 검증하고자 하였다. 방법 : 인체에서 수막염을 일으킨 다제내성 폐렴구균 균주(penicillin MIC 2, ceftriaxone MIC 4, meropenem MIC 0.5㎍/㎖)를 토끼 척수강 내에 직접 주입하여 수막염을 유발한 후 ceftriaxone, vancomycin, rifampin, meropenem의 단일 항균요법, ceftriaxone+vancomycin, ceftriaxone+rifampin, meropenem+vancomycin의 병용요법 간에 치료 효과의 차이를 비교 하였다. 각 치료군은 6 마리의 토끼를 대상으로 하였다. 각각의 항생제는 5 시간 간격으로 2번 정주하였으며, ceftriaxone은 1회 투여하였다. 균주 주입 후 각 치료군이 토끼에서 치료 후 0, 5, 10, 24 시간에 척수액을 채취하여 균주의 수를 측정하여 24시간째의 살균 여부를 최종 치료 효과로 판정하였다. 결과 : meropenem 단독 요법은 10시간째에 모든 균주를 살균하였으나 24시간 째에 다시 균의 성장이 관찰되었으며, meropenem+vancomycin의 병용요법은 단독 약제보다 우수한 살균효과를 보였으나 상승작용은 없었다. 결론 : meropenem+vancomycin의 병용요법은 기존의 ceftriaxone+vancomycin보다 24시간째에 더 큰 균의 감소 경향을 보여(P=0.054) 임상에서 사용이 가능할 것으로 기대되며 향후 실제 수막염 환자에서의 임상효과를 증명하는 연구가 필요할 것으로 생각된다. Background : Because antimicrobial monotherapy for pneumococcal meningitis caused by penicillin-resistant strains is not satisfactory, ceftriaxone+vancomycin [C+V] or ceftriaxone+rifampin [C+R] regimens are recommended. Meropenern [M] is one of the monotherapy options for penicillin-resistant pneumococcal meningitis due to good in-vitro activity against pneumococci and rare adverse reactions in CNS. But there have been few reports in the efficacy of meropenern against pneumococcal meningitis. We evaluated therapeutic efficacy of the meropenern and meropenem+vancomycin in a rabbit model of meningitis caused by penicillin-resistant S. pneumoniae (PRSP). Methods : Meningitis was induced by intracistemal inoculation of a pneumococcal strain isolated from a patient with meningitis (MIC; penicillin 2, ceftriaxone 4, meropenem 0.5 g/㎖). Bacterial concentrations in the CSF were measured at 0, 5, 10, and 24 h after therapy was started. Therapeutic efficacy was evaluated by the final bacterial concentration in the CSF at 24 h. Results : C+V cleared the CSF at 10 h, but regrowth of bacteria was noted at 24 h. Meropenem monotherapy resulted in sterilization at 10 h but regrowth at 24 h. M+V was superior to M or V monotherapy but did not show synergism. Therapeutic efficacy of M+V was at least equal or superior to that of C+V (P = 0.054). Conclusion : Meropenem+vancomycin regimen could be one of the useful options in the treatment of PRSP meningitis. Clinical trials to evaluate the M or M +V are warranted in the future.

Theoretical studies on in-plane polarization characteristics of (11$$\bar{2}$$0) nonpolar InGaN/GaN quantum-well structures grown on InGaN substrates

박승환,Shim Jong-In,Shin Dong-Soo 한국물리학회 2022 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.81 No.1

The in-plane polarization characteristics of nonpolar (11̄2 0) InxGa1−xN/GaN ( x = 0.2 ) quantum-well (QW) structures are investigated as a function of In content in InyGa1−y N substrates by using the multiband effective-mass theory. States constituting the topmost valence subband change from Y >-like to Z >-like as the In content in the InGaN substrate increases. In the case of the QW structure grown on a conventional GaN substrate ( y = 0.0 ), the y-polarized matrix element is much larger than the x-polarized matrix element. However, the y-polarized matrix element rapidly decreases with increasing y content in the InGaN substrate and becomes similar to the x-polarized matrix element. As a result, the magnitude of the in-plane optical anisotropy becomes smaller for QW structures with higher substrate In content because both x - and y -polarized emission peaks are similar to each other.

綜合病院 로비空間의 構造化에 관한 硏究

모신희,나승문,김수인 朝鮮大學校 建設技術硏究所 2007 建設技術硏究 Vol.27 No.2

This study is based on user's awareness of space in regard in to "medical welfare facilities" among local facilities. It researches "structuralization on lobby space" in a hospital construction which has gigantic and comprehensive systematic character. It grasped and explored user's awareness to the lobby space, which can be applied to architectural plan data for the designing of future hospital construction. The research has been made by conducting 16 general hospital located in 6 administrative regions in Gwang ju Metropolitan City. Constant research has been done in many fields at hospital construction, and progress has been made repeatedly in line with the current request. In addition, "a hospital is referred to as art", and hospital construction as the facility as hospital quality or growth meaning is considered as different from other area This study is aimed at investigating spatial nature to the lobby space of a large city general hospital, and trying the space itself, and finding out new conceptual establishment of the lobby space and the architectural spatial nature of the lobby through positive facts to some factors the sturucturalization attempted th explore the awareness ingrained in the users(doctors, managers, employees and patient). As a result, hospital construction could materialize the spatial concept in construction planning, through which we could find it as the space th complexly accept the factors of colorful forms, and elicit the unit spatial scale in programming, and quantify the spatial volume sense with the consciousness inherited in sentimental engineering factor.

국내 5개 3차 병원의 β-lactam계 항생제 사용 실태 및 Klebsiella pneumoniae의 항생제 내성과의 상관 관계

정숙인,박경화,권기태,고관수,오원섭,정두련,백경란,염준섭,장현하,김신우,손준성,송재훈 대한감염학회 2007 감염과 화학요법 Vol.39 No.4

Background : This study was performed to evaluate the prescribing pattern of β-lactam antibiotics and the relationship between antimicrobial resistance and antimicrobial use in 5 tertiary care hospitals in Korea. Materials and Methods : Data on annual patient-days and annual consumption (defined daily dose (DDD) per 100 patient-days) of β-lactam antibiotics from 2003 to 2005 in 5 tertiary hospitals were analyzed. To determine the antimicrobial resistance in K. pneumoniae, broth microdilution test and double disk synergy were performed according to the CLSI performance standards. Spearman's correlation coeffient was used to determine the relationship between antibiotic consumption and resistance. Results : The prescription of β-lactam antibiotics in 5 tertiary hospitals markedly varied. In two hospitals, increase in consumption of β-lactam antibiotics was more than 30% during recent 3 years. The higher consumption of β-lactam antibiotics significantly correlated with the higher rate of antibiotic-resistant K. pneumoniae in 5 tertiary care hospitals. Conclusion : Continuous surveillance of antibiotic use is needed to encourage appropriate prescribing of antibiotics and to reduce antibiotic resistance. 목적 : 항생제의 오남용으로 인한 내성균의 발현 및 급속한 확산이 전세계적으로 문제시 되고 있다. 특히 병원은 항생제 내성의 중심점에 있는 것으로 여겨진다. 본 연구에서는 최근 3년간 국내 5개 3차 병원의 β-lactam계 항생제의 사용량을 조사하고, 각 병원별 항생제 사용량과 K. pneumoniae의 항생제 내성률과의 상관관계를 알아보고자 하였다. 재료 및 방법 : 국내 5개 3차 병원에서 2003년 1월부터 2005년 12월까지 병원 입원 환자를 대상으로 연도별 재원연인원수와 각 β-lactam계 항생제의 총사용량을 수집하여 DDD (defined daily dose) per 100 patient-days로 환산하여 비교하였다. 2005년 6월부터 8월까지 각 병원의 임상검체에서 동정된 K. pneumoniae를 수집하여 액체배지미량희석법으로 항생제 감수성 검사를 시행하였고 ESBL(extended-spectrum β-lactamase) 생성 여부는 double disk synergy test를 시행하였다. 항생제 사용량과 내성의 상관관계는 Spearman 상관관계 분석을 시행하였다. 결과 : 5개 병원 중 3개 병원에서는 연도별 β-lactam계 항생제 사용량이 비슷하게 유지되었으나, 2개 병원에서는 2003년에 비해 2005년에 항생제 사용량이 30% 이상 증가하였다. β-lactam계 항생제 중 제제별 비율은 각 병원마다 차이가 있었으나, 3세대 cephaosporin의 사용이 가장 많았고 2세대 ceaphlosporin, β-lactam/β-lactamase inhibitor, 1세대 cephalosporin 순이었다. 경구용 β-1actam계 항생제의 사용량(r=0.900, P<0.037)과 2세대 cephalosporin의 사용량(r=0.900, P<0.037)은 각각 K. pneumoniae의 ESBL 생성과 양의 상관관계가 있었다. Ceftazidime의 사용량은 K. pneumoniae의 ceftazidime 내성률과 양의 상관관계가 있었다(r=0.900, P<0.037). 반면 imipenem의 사용량은 piperacillin/tazobactam 내성과 음의 상관관계가 있었다(r=-0.900, P<0.037). 결론 : 최근 3개년간 항생제 사용량은 병원별로 차이가 있으나 일부 병원에서는 증가하였다. β-lactam계 항생제의 사용량은 K. pneumoniae의 항생제 내성과 상관관계가 있었다. 항생제 사용을 줄임으로써 항생제 내성을 감소시킬 수 있을 것으로 기대되며, 병원별로 항생제 사용에 대한 지속적인 감시가 필요할 것이다.