White luminescence of Ho3+/Tm3+/Yb3+-codoped CaWO4 synthesized via citrate complex route assisted by microwave irradiation

CHO, Hyun,HWANG, Seok Min,LEE, Jae Bin,KA, Dong Hyun,KIM, Tae Wan,LEE, Bo Seul,LEE, Ji Young,LEE, Jung-Il,RYU, Jeong Ho Elsevier 2014 Transactions of Nonferrous Metals Society of China Vol.24 No.suppl1

FCT 5 : Fractional laser-assisted topical imiquimod 5% cream treatment for recalcitrant common warts in children

( Hyun Chang Ko ),( Jeong Min Kim ),( Hyun Ho Cho1 ),( Won Jeong Kim ),( Je Ho Mun ),( Margaret Song ),( Hoon Soo Kim ),( Byung Soo Kim ),( Moon Bum Kim ) 대한피부과학회 2013 대한피부과학회 학술발표대회집 Vol.65 No.2

Background: Conventional destructive modalities for warts like cryotherapy or laser ablation have some limitations that are excruciating pain during procedure, in especially pediatric patients. Imiquimod is a topical immune response modifier that was approved for treating genital and perianal warts. But, thick stratum corneum of common warts may act as a barrier against drug permeation via the skin. Objectives: To evaluate efficacy and safety of above treatment. Methods: Eleven pediatric patients (6 female and 5 male, mean age 12.5 years) were included in this study. The lesions were treated by fractional 2940 nm Er:YAG laser for achieving the penetration of stratum corneum with 1 or 2 weeks intervals. Then, imiquimod 5% cream was self-applied once daily for 5 days a week. Assessment of response and adverse effects was performed every 2 weeks until complete clearance or up to maximum of 48 weeks. Pain during procedure was checked by VAS (0-10). Results: 8 out of 11 (72.7%) patients experienced complete clearance of all warts lesions. Mean duration of total treatment was 29.7 (16-48) weeks. Mean number of fractional laser treatment was 17.5 (8-37) times. No significant adverse effect was observed. VAS score of the pain during fractional laser treatment was 2.4 (1-4), comparing with 6.2 (5-8) during cryotherapy. Conclusion: Fractional ErYAG laser-assisted topical 5% imiquimod cream is an effective and safe treatment option for recalcitrant common warts of the children do not tolerate pain well.

RAPD分析法에 의한 참나무 優良種의 PCR反應 分析

趙顯瑞,李喆浩,李正煥 진주산업대학교 1999 산업과학기술연구소보 Vol.- No.6

RAPD를 이용한 참나무류의 band pattern은 primer의 종류에 따라 공시 수종별로 구분이 가능한 표식인자(marker)를 가지고 있었고, 상수리나무 (lane 1-11) band pattern에서는 고유 band를 보였다. Primer OPA-10, OPA-16에서 갈졸참나무 (lane 28)은 갈참나무(lane 22-25) 및 졸참나무 (lane 12-17)와 공유 band가 있었으마, 갈참나무와 유사한 band pattern을 보여 갈참나무와 유사한 경향을 보였다. 촐참나무 (lane 12∼17), 갈참나무 (lane23∼27)와 갈졸참나무(lane28) 집단 간에는 유사한 band pattern을 보여 갈졸참나무가 갈참나무에 근접한 교잡종으로 인식되었다. 군집분석결과 28개 집단은 7개 Grpup로 분류되었는데, 제1 집단은 상수리나무, 제 2 집단은 갈참나무, 제 3집단은 졸참나무 등으로 집단간 종의 구분이 가능하였다. In the Polymerase Chain Reaction (PCR) - Random Amplified Polymerase (RAPD) DNA analysis was performed on DNA samples obtained from leaf collected from wild Quercus spp. in Kyeongnam Province. PCR - DNA markers were used as a predictor of superior tree in future Quercus spp. breeding development. Eight primers were used for PCR and generated a total of 122 RAPD markers. According to the PCR products bands of Q. acutissima (lane 1-11), Q. serrata (lane 12-17), Q. uariabilis (lane 18-22), Q. aliena (lane 23-27) a n d Quercus x urticaefolia (lane28) were detected in all samples. Genetic relationships among the 28 individual samples were analyzed by the cluster analysis of 122 RAPD markers, which divides seven groups. According to the cluster analyses, First group are contains Q. acrtissima. Second group are Q. aliena, third group Q. serrata and so on. And close relationship between Quercus x urticaefolia and Q. aliena were ascertained by cluster analysis.

이중에너지 방사선 흡수계측법을 이용한 성인 남녀의 장골 및 경골의 골밀도에 관한 연구

조용석,김경원,이경호,박현진,서상수,오상엽 대한악안면성형재건외과학회 2000 Maxillofacial Plastic Reconstructive Surgery Vol.22 No.3

Purpose : The aim of this study was to evaluate the bone mineral density of iliac and tibial bone which are frequently selected as autogenic bone graft donor sites, and to evaluate the efficiency of this method as a guideline for the selection of bone graft donor site. Materials and Methods : In this study 61 male and 70 female volunteers at Chungbuk National University Hospital were involved between Jan. 1998 to Sept. 1999. We measured bone mineral density of the iliac and the tibial bone using dual energy X-ray absorptiometry. We evaluated the data using the SAS system for Windows and bone mineral density of the lumbar was used for control. Results : Age showed the highest correlation in correlation matrix between physical and bone variables. Height and weight showed lower correlation of linear increment. In man, the change of bone density according to age demonstrated linear decrease irrespective of the lumbar, ilium, and tibia. In woman, the change of bone density according to age showed cubic form, which increased in the third and forth decade. So it had a peak bone mass on about 35 year-old, thereafter, the change of bone density slowly decreased until 50 year-old, but it rapidly decreased after 50 year-old and it slowly decreased again after 65 year-old. Both in all subjects and subject with osteoporosis, the change of bone mineral density according to age showed statistically significant decrease in lumbar and tibia, but ilium was irrespective. Conclusion : In patients of aged or with osteoporosis, ilium demonstrated less tendency of decrease in bone mineral density than tibla. So this preliminary study suggested that ilium seemed better donor site for autogenic bone graft than tibia.

참나무 群落地의 植物群集構造分析 : Ⅰ. 白雲山의 植物群集構造分析 Ⅰ. Plant Distribution and vegetation structure in Mt. Bakwoon

趙顯瑞,李喆浩 진주산업대학교 1998 論文集 Vol.37 No.-

백운산 내회지역과 지리산 대원계곡의 삼림군집구조를 파악하여 우량종 육성에 필요한 자료를 분석한 결과는 다음과 같다. 1. 백운산지역 95개 조사지를 TWINSPAN에 의하여 classification을 분석한 결과, 남사면에서는 양수인 소나무 군집으로 구분되었으며, 북사면에서는 활엽수흔효군집으로 구분되었다. 2. 백운산지역 조사지는 DCA에 의하여 1, 2축에 우측에서 좌측으로 소나무군집, 굴참무군집, 졸참나무-굴참나무군집, 졸참나무군집, 서어나무군집으로 분리되었고, 상, 하로 신갈나무군집, 활엽수흔효군집, 고로쇠나무군집 등의 8개 군집으로 분리되어 TWINSPAN보다 DCA방법이 효과적이었다. 3. 백운산 지역 군집간의 유사도지수를 분석한 결과, 소나군집과 졸참나무군집, 소나무군집과 서어나무군집, 소나무군집과 고로쇠나무군집, 신갈나무군집과 고로쇠나무군집의 유사도지수가 20%미만을 나타내어 이질적인 군집임을 알 수 있었고, 졸참나무-굴참나무군집과 굴참나무군집, 졸참나무군집, 활엽수흔효군집 그리고 굴참나무군집과 활엽수흔효군집간에는 55% 이상의 높은 값을 나타내었다. 4. 백운산지역 토양 환경인자의 DCA분석에서 토양산도를 제외하고 모든 인자의 A군집(소나무군집)에서 E군집(서어나무군집)으로 갈수록 증가하는 경향이었다. 5. 백운산지역 토양산도, 수분함량, 유기물함량 및 K^+, Ca^++, Mg^++ 등 토양환경요인을 DCA기법을 적용한 결과, 대부분의 요인에서 소나무, 신갈나무군집은 낮은 계급을 나타내었고, 서어나무군집, 활엽수흔효군집, 고로쇠나무군집 등에서는 높은 계급에 위치하고 있었다. This paper aims at analyzing the plant community in Mt. Bakwoon area. The plant community in Mt. Bakwoon was divided into 95 plots by clumped sampling method. According to DCA ordination, Mt. Bakwoon is composed of Pinus densiflora, Quercus variabilis, and Q. serrata communities. The similarity index between Quercus variabilis and mixed stands of broad leaved trees was more than 55%.

우울증 관련 평가도구의 개관

조현주,채정호,전태연 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.2

Objective : Since a lot of assessment tools for depression have been developed and used, it is difficult to choose the proper and optimal instruments for the purpose of their usage, In order to maximize the effectiveness of assessment for depression, this paper reviewed the characteristics and specific features of currently using tools. Method : The domestically and internationally using assessment tools for depression were reviewed and classified by litera-hire search and clinical experience. Results : Methods of assessment, selecting guides and characteristics of assessment tools for depression were presented. Conclusion : This paper would provide helpful guidelines for selecting the assessment tools. We suggest that it is needed to develop the assessment tools for depression that reflects Korean specific culture and custom.

Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.

세침 흡인술을 이용한 종창된 림프절의 세포학적 평가

백강현,윤기영,최호정,정성목,이영원,조성환,박성준 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2011 動物醫科學硏究誌 Vol.18 No.1

Fine needle aspiration(FNA) cytology is considered as the diagnostic technique of choice in the assessment of swellen lymph nodes. The aim of this study is to determine the indication and diagnostic method of FNA of lymph node lesions performed at Veterinary Medicine Teaching Hospital of Chungnam National University. The records of 16 patients which had undergone FNA during the study period were retrieved. The cytological results were classified as lymphadenitis, lymphoid hyperplasia and lymphoma. Lymphoma cases were classified as B-cell origin and T-cell by immunophenotyping. The result of the FNA cytological diagnosis showed that nine (56%) of the patients were lymphoma, five (31%) of the patients were lymphoid hyperplasia and two (13%) of the patients were lymphadenitis.

Cycloheximide가 Mouse肝臟의 인산염 分解酵素의 活性에 미치는 影響

趙賢熙,鄭鎬三,李圭植 漢陽大學校 環境科學硏究所 1985 環境科學論文集 Vol.6 No.-

Cycloheximide는 Streptomyces griseus에서 추출한 항진균성 항생제로 진균의 성장을 저해하는 물질로 알려져 왔으며 포유동물의 세포에서 ribonucleic acid와 단백질 합성을 저해한다고 생화학적 방법에 의하여 보고되었다. 최근에 이르러 cycloheximide의 투여로 간세포내 소기관인 조면내형질세망 및 Golgi complex의 손상에 의해서 단백질합성이 저해된다고 형태학적으로 연구 보고된 바 있다. 이와같은 cycloheximide의 투여로 간세포가 손상을 입어 단백질합성이 저해될 때 간세포내 인산염분해효소로 분포되어 있는 acid phosphatase와 adenosine triphosphatase 활성을 추구하였다. 실험동물로는 실험실에서 사육한 건강한 ICR계 체중 20∼30gm의 mouse를 사용하였으며 정상대조군과 cycloheximide 투여군으로 구분하고 실험군은 다시 cycloheximide 투여 후 6시간군, 12시간군, 24시산군 및 36시간군으로 나누었고 각군에 10마리씩 배정하였다. Cycloheximide는 mouse의 체중 ㎏당 4㎎ 되게 생리적 식염수에 용해하여 0.1㎖를 복강내에 투여후 6시간, 12시간, 24시간 및 36시간 후에 희생시켜 간장을 적출하고 10% neutral formalin에 고정한 다음 cryostat로 두께 15㎛ 되게 동결절편을 만들어 Gomori법으로 acid phosphatase활성과 Wachstein 및 Meisel법으로 adenosine triphosphatase 활성을 관찰한 결과 다음과 같은 결과를 얻었다. 1. Cycloheximide 투여후 6시간, 12시간군 및 24시간군의 mouse 간소엽에서 중심 정맥부로 부터acid phosphatase 활성이 증가되기 시작하여 36시간군에서는 간소엽 전역에 중등도 이상의 강한 활성이 관찰되었다. 2. Cycloheximide 투여후 24시간군에서 부터 간소엽의 중심 정맥부에 ATPase 활성이 감소되기시작하여 36시간 경과군에서는 간소엽 전역에 음성반응을 나타내었다. 상기와 같은 결과는 cycloheximide 투여로 간장의 acid phosphatase는 간장이 손상을 입을 때 그 활성이 보다 강한 양성반응을 보이고 adenosine triphosphatase는 역으로 활성이 약해진다고 사료된다. It has been knwon that cycloheximide, an antifungal antibiotics extracted from streptomyces griseus, inhibits the growth of fungus. It has been reported by several investigators that this drug inhibits ribouncleic acid activity and protein synthesis of the mammalian cell. Recently, several reporters indicated that the cycloheximide causes the damage of hepatocyte. The observations by electron microscopic, histochemical and histological studies revealed that cycloheximide inhibits the RNA and protein synthesis by the injury of rough endoplasmic reticulum and the desocciation of polysome in hepatocyte. This study attempted, on the activities of acid phosphatase adenosine triphosphatase, phosphatelysis-enzymes distributed in the hepatocytes in the mouse liver. Healthy albino mice(ICR strain) of 20gm±in body weight were used as experimental animals which were divided into the control and cycloheximide treated groups. The animals of the cycloheximide treated groups were administered cycloheximide. (dissolved in physiological saline sol., by intraperitoneal injection in a dose of) 4mg per kg of body weight in a volume of 0.1ml, intraperitoneally. The experimental animals were sacrificed at intervals of 6, 12, 24 and 36 hours after administration of cycloheximide, respectively. The liver specimens were fixed with 10% neutral formalin, the sections of 15㎛ thickness were stained by Gomori, Wachstein and Meisel`s method for the detection of acid phosphatase and adenosine triphosphatase activities. The results were obtained as follows; 1. Acid phosphatase activity was increased in the central zone of liver lobule at the 6 hour and 24 hour period of the cycloheximide treated group, moderately positive activity was showed in the liver lobule at the 36 hour period group. 2. Adenosine triphosphatase activity was decressed in the centrol zone of liver lobule at the 24 hour period of the cycloheximide treated group. Negative reactions were showed in the whole liver lobules at the 36 hour period group. Consequently, it is supposed that cycloheximide caused increase of liver acid phosphatase result from liver damage, while adenosine phosphtase activity of the liver was decreased.

연쇄상구균을 실험감염시킨 넙치치어의 병리조직학적 관찰

조성환,류시윤,김현수,박배근,김덕환,손화영,윤원기,신창호 충남대학교 수의과대학 동물의과학연구소 1999 動物醫科學硏究誌 Vol.7 No.-

Pathological observations were examined after abdominal inoculation in 7∼8㎝ sized flounder (Paralichthys olivaceus) with 1X10^7 cfu/㎖ of Streptococcus faecalis. The infected flounder were clinically observed and weighed a fish body at 0, 5, 10, 15, 20, 25, 30 days post inoculation. In infected group, mortality were 70% and feed efficiency were 7.2%. In non-infected group, mortality were 10% and feed efficiency were 18.2%. Macroscopically, the significant lesions were characterized by protrusions and opacity of the eyes and ulcer of the caudal pecuncle. Histopathologically, the lesions were observed a degeneration. necrosis and inflammatory cells in muscle of the caudal peduncle and a degeneration and necrosis of the rod cells and detached retina from the black pigmented layer