蛙 脾臟의 組織學的 硏究

鄭鎬三,林洛龍 최신의학사 1975 最新醫學 Vol.18 No.3

Although frog spleen is generally structurally similar to that of mammals, the following differences were observed in our study. . 1. There is only one trabecula in the middle zone of tissue, and the capsule of the frog spleen does not contain smooth muscle fibers. . 2. The white pulp does not have uniform complete nodules, the lymphocytes in the white pulp are not packed close together, the white pulp contains large numbers of erythrocytes, and there are no central arteries or germinal centers in the spleen of frogs. 3. The arteries of frog spleen, unlike those in mammalian spleen, do not branch into central arteries in the white pulp, or into sheathed arteries in the red pulp. 4. Reticular tissue appears near the venous sinuses, but is not widely distributed throughout other splenic tissue. 5. Veins of frog's spleen are distributed beneath the capsule and periphery of trabecula.

[지상27]Adriamycin 투여후 흰쥐 왼심실근육층에서 세포자멸사의 발현에 미치는 Vitamin C의 영향

정호삼,서윤경,백두진 대한체질인류학회 2003 대한체질인류학회 학술대회 연제 초록 Vol.46 No.-

cis-Dichlorodiammineplatinum(Ⅱ)이 발정주기에 따른 흰쥐 난관섬모 세포의 형태 및 섬모형성에 미치는 영향에 관한 전자현미경적 및 면역조직화학적 연구

김동옥,송양주,전영희,정호삼 한양대학교 의과대학 1995 한양의대 학술지 Vol.15 No.1

electron microscope for morphological changes of cytoplasmic organelles and with light microscope for detection of tubulin substances in the cytoplasm during estrous cycle. Experimental animals(weighting 200gm. female albino rats) divided into 4 groups by the estrous cycles. Ampullar oviducts of these animals were excised at each estrous cycles. The specimen was made into immunocytological reaction slides for detection of tubulin in the cytoplasm, and the other specimen were made to ultrathin section for electron microscopy. All of specimens were examined with light and electron microscope. The results obtained are as follows. 1. The tubulin substances in ciliated cells of oviducts revealed strong reactions during proestrus and estrus but weak reactions during metestus and diestrus. 2. After cis-Platin treatment, tubulin substances in the cytoplasm of oviductal ciliated cells showed weak stain reactions but at diestrus, moderate reactions were seen similar to that of the control group. 3. The cilia and basal bodies of ciliated cells of ampulla oviducts after cisplatin treatment were decreased in number at all stages of estrous cycle. 4. Ciliated cells in cis-Platin treated rat revealed hypertropies of mitochondria, atrophies of Golgi complex, decrease of polyribosomes and segmented cisternae of rough endoplasmic reticulum at proestrus, estrus and metestrus. It is consequently suggested that cis-Platin would induce inhibition of ciliogenesis in oviductal ciliated cells during estrous cycles.

Retinoic acid가 흰쥐태자의 허근육의 분화와 Laminin 합성에 미치는 영향

정호삼,김원규,황규성,서윤경,윤지회,백두진,김혜주 대한체질인류학회 2000 대한체질인류학회 학술대회 연제 초록 Vol.43 No.-

흰쥐의 허파분화 과정에서 Laminin 활성의 변화에 대한 면역조직화학적 및 면역도금법에 의한 연구

정호삼,서윤경,김원규,윤지희,백두진,박철홍 대한체질인류학회 2000 대한체질인류학회 학술대회 연제 초록 Vol.43 No.-

Interleukin-2가 Mouse 간실질세포의 미세구조에 미치는 영향

손승국,백태경,이규식,정호삼 한양대학교 의과대학 1990 한양의대 학술지 Vol.10 No.1

It is well known that interleukin-2, a kind of lymphokine produced by the lymphocytes stimulated by antigen or mitogen, mediates antitumor effects by stimulating the proliferation of helper and cytotoxic T-cells, augmenting the tumorlytic activities of natural killer cell and promoting the differentiation of precusor T-cell into lymphokine activated killer cell in vivo. Thus interleukin-2 has been widely used as immunotherapeutic agent for various sarcoma and carcinoma and also regressed the metastasis of malignant tumors. Although interleukin-2 is beneficial to cancer patient, it also develops many toxic effects such as fever, chillness, general malaise, mental confusion, vomiting, diarrhea, hepatic and renal dysfunction, and capillary leakage syndrome, including the general edema, heavy weight gain, ascites any pulmonary edema, which limits clinical use of interleukin-2. The author undertook this experiment to pursue the effect of interleukin-2 on the ultrastructure of hepatocyte in mice. A total of 75 healthy male DDY mice, weighing about 20gm, were used as experimental animals. The experimental animals were divided into the control and interleukin-2 treated groups, and then interleukin-2 treated mice were subdivided into 6 hours, 12 hours, 24 hours and 48 hours groups by intervals of time after administration and each group allocated 15 mice. Mice of interleukin-2 treated group were administrated with 2 million unit of interleukin-2, per kg of body weight, diluted in intraperitoneally. Animals were sacrificed 6,12,24 and 48 hours after administration. The specimens, left anterior hepatic lobe, were prefixed in 2% glutaraldehyde-2,\.5% paraformaldehyde solution buffered by Millonig's phosphate buffer (pH 7.2) for 2-4 hours and postfixed in 1% osmium tetroxide buffered by same buffer solution for 2 hours at 4℃. After dehydration, the specimens were embedded in Epon 812 and the ultrathin sections, 600-800A inthickness, were made with LKB V ultramicrotome. The preparations were stained with uranyl acetate and lead citrate and observed with JEM 100cx-II electron microscope. The result obtained were as follows; 1. Dilation, sacculation and disruption of cisternae of rough endoplasmic reticulum accompanied with dissociation of membrane bound ribosome were observed but as time goes by, these changes were recovered. 2. Disorganization of mitochondria was observed. 3. Proliferations of smooth endoplasmic reticulum associated with depletion of glycogen particles was recognized. But as time goes by, the glycogen particles were represented. 4. Numerous variously sized autophagic vacupoles and multivesicular bodies were observed. 5. Numerous lipid droplets were observed but they were disappeared as time goes by. Based on the result obtained, it is suggested that interleukin-2 would induce changes of ultrastructures of hepatocyte in mouse but these changes were recovered as time goes by.

일산화탄소 폭로후 고압산소 재폭로시 흰쥐 신장 및 심장의 SOD 활성 변동에 관한 연구

전영희,백두진,황세진,박준숙,정호삼 한양대학교 의과대학 1997 한양의대 학술지 Vol.17 No.1

Carbon monoxide is generated from the incomplete combustion of carbon-containing materials. Carbon monoxide cause muscle necrosis leading to myoglobinuria and acute renal failure. The hyperbaric oxygen exposure is increased production of active free radicals. Free radicals which promoted peroxidation of unsaturated fatty acids may destroy essential cellular components and itself cytotoxic materials. Superoxide dismutase, one of the protective mechanisms against tissue damage, catalyzed the dismutation of superoxide anion to less toxic product. The author performs the present study to investigate on Mn-SOD and Cu, Zn-SOD immunoreactivities in kidney and heart carbon monoxide (CO) and hyperbaric oxygen (HBO) exposure. The animals were divided CO exposure group, air release after CO exposure group, HBO exposure group and, CO and HBO exposure group. The specimen were sectioned in 16㎛ with cryostate. The Mn-SOD and CuZn-SOD immunohistochemistry was performed in renal corpuscle, proximal and distal convoluted tubules of kidney and, in sarcoplasm and sarcolemma of heart muscle. The results obtained were as follows: 1. The immunoreactivity of Mn-SOD were increased in renal corpuscles and distal convoluted tubules, were similar to control group in proximal convoluted tubules. 2. The immunoreactivity of Cu, Zn-SOD were increased than that of control group in renal corpuscles and distal convoluted tubules, were same that of the control group in proximal convouted tubules. 3. The immunoreactivity of Mn-SOD were increased at all experimental group of sarcoplasms of heart muscle. 4. The immunoreactivity of Cu,Zn-SOD were increased than that of control group in sarcolemma of heart muscle. These results suggest that the immunoreactivity of SOD after CO or HBO exposure is greatly increased in kidney and heart.

Interleukin-2가 Mouse 비장의 혈구전구체세포에 미치는 영향에 관한 전자현미경적 연구

정호삼 한양대학교 의과대학 1989 한양의대 학술지 Vol.9 No.2

Interleukin-2, T-cell growth factor, is a glycoprotein produced by the lymphocytes and it is widely used as an immunotherapeutic agent on the hepatoma and various carcinoma. Interleukin-2 mediate antitumor effect by stimulating the proliferation of helper and cytotoxic T-cells and increasing the generation of lymphokine activated killer cells. Thus interleukin-2 was administered to the cancer patients and experimental animals, it could also effect on the lymphoblasts and reticular cells in the spleen. In this experiment, the author pursued the effect of interleukin-2 on the ultrastructure of the reticular cell of the mouse spleen. Albino mice, DDY strain, weighing 20gm were used as experimental animals. The experimental animals were killed at 3, 6, 12, 24 and 48 hours after administration of 2,000,000 unit/kg interleukin-2. The specimens obtained from the spleen were prefixed in 2% glutaraldehyde-2.5% paraformaldehyde solution and postfixed in the 1% osmic acid. After dehydration, the specimen were embedded in Epon 812 and then ultrathin sections(600-800A??) were made and stained with urarnyl acetate and lead nitrate. And these preparations were observed with JEM 100cx-II electron microscope. The results were as follows. 1. Medium sized lymphocytes, large lymphocytes and reticular cells were increased in the white pulp of the 3, 6 and 12 hours interleukin-2 treated mouse spleen. A few rod shaped rough endoplasmic reticulum were observed and mitochondria and vesicles were abundant in its cytoplasm. 2. Small lymphocytes, medium sized lymphocytes, large lymphocytes and reticular cells were seen as regular shapes and their cytoplasmic organelles were normal in the white pulp of the spleen in 24 and 48 hour interleukin-2 treated Mice group. Consequently, it is suggested that interleukin-2 would induce the development of the cytoplasmic organelles in the reticular cells but the cytoplasmic organelles in the reticular cells would return to its normal state as the time goes by.

[지상33]비타민 C가 Adriamycin을 투여한 흰쥐 심장근육에서 NF-κB, AP-1, Bcl-2 Bax 와 의 분포에 미치는 영향

정호삼,조용범,백두진 대한체질인류학회 2004 대한체질인류학회 학술대회 연제 초록 Vol.47 No.-

新生白鼠의 脾臟에서 白髓毛細血管과 白髓小器管의 分化

鄭鎬三 漢陽大學校 1979 論文集 Vol.13 No.-

1. 흰쥐의 비장이 백수모세혈관을 출생후 제3일에 적수내에 분포되어 있는 필모동맥에서 분지되여 백수의 전구체인 동맥주위임파구초내로 분포된다. 2. 백수모세혈관은 적어도 출생후 제13일 이후에 백수의 중심동맥에서 분지된다. 3. 출생후 제3일에 세망섬유가 동맥주위임파구초에 형성되고, 출생후 제7일에 세망구조가 성숙 흰쥐의 비장의 그것과 거의 동일하게 형성된다. 4. 출생후 제14일에 변연대와 변연대동이 전형적으로 형성되며 백수모세혈관과 변연대동의 연결이 관찰된다. 그리고 배아중심도 이어서 형성되며 성숙 흰쥐 비장의 백수와 같은 구조가 형성된다. THE DEVELOPMENTS OF THE ORGANELLES IN THE SPLEEN ================================================================================ site Periarterial Develoment Develoment Develoment Develoment Develoment The day lymphatic of of of of of follicular after birth sheath reticulum marginal zone marginal sinus germinal ceter capillary -------------------------------------------------------------------------------- ++ - - - - - 1st day ++ - - - - - 2nd day ++ + - - - - 3rd day +++ ++ - - - + 4th day +++ ++ - - - + 5th day +++ ++ - - - + 6th day +++ ++ - - - + 7th day +++ +++ - - - + 10th day +++ +++ + + - + 13th day +++ +++ + + - + 14th day +++ +++ ++ ++ + + 15th day +++ +++ ++ ++ ++ ++ 21th day +++ +++ ++ ++ ++ ++ 33th day +++ +++ +++ +++ +++ +++ -------------------------------------------------------------------------------- Foot note: -undevelopment +poor development ++moderate development +++complete development The connection, the histological architecture and the distribution of the follicular capillaries and marginal sinuses in the white pulp of the spleen have a matter of general interest in relation to the production of the antobody in the spleen. It is reported that the histologists in the field of the spleen studies (Altschul and Hummason'47, Andrew'46, Snook'44 '50 '58 '64, Galindo and Imaeda '62 '63 and WEISS '73)have defined that the follicular capillaries are branched from the central arteries of the white pulp and that the blood from the central arteries are supplied into the germinal center and maeginal zones. As follows, a series of the different results have been obtained in the present study of te developing spleen tssues of the neonatal albino rats. 1. The capillaries from the central artery in the neonatal rat spleen did not branch within 13 days after birth. 2. At the stage of the 3rd day after birth, the reticular fibers in the white pulp began to form; and at the stage of 7th day after birth, a reicular fiber formation was almost as perfect as that of the adult rat. 3. At the stage of the 3rd day after birth, the follicular capillaries began to branch from the penicillar arterioles in the zone between the periphery of ,the white pulp and the red pulp. 4. At thestage of 14th day after birth, the formation of the marginal zones and marginal sinues were completed; and the follicular capillaries and marginal sinues began to anastomose in the space of the marginal zones of the spleen. 5. At the stage of 14th day after birth, the central artery began to locate peripherally in the white pulp; and ,the germinal center was formed completely.