국립공원 구역 조정에 따른 환경,경제,사회적 파급효과 분석

홍현정 ( Hyun Jung Hong ),최현아 ( Hyun Ah Choi ),변병설 ( Byung Seol Byun ),박용하 ( Yong Ha Park ) 한국환경복원기술학회(구 한국환경복원녹화기술학회) 2013 한국환경복원기술학회지 Vol.16 No.6

As ten years have passed since national parks were adjusted and released inceptively, it is the time for evaluating the effectiveness of the national park policy and improving it on the basis of the evaluation result. In this study, the method of appraising the environmental and socio-economical ripple effect on the implementation of national park regulation were provided, based on the institutional consideration, the environment analysis, the survey of residents consciousness, the statistical analysis and so on. It is applied to target sites where were released from national parks, and the policy direction was suggested for sustainable use and management of national parks. National parks, that exhibit various characteristics depending on geographical and local conditions, were categorized through the statistical approach. Released sites from Seoraksan National park, where was categorized as the core national park for sustainable use and management, were evaluated environmentally based on the time-series analysis of environmental thematic maps and socio-economically based on the survey on residents perceptions. As a result of this study, the environmental effect of released sites has been declined or threatened, and the residents` economic, social and living condition in these areas are still not improved(p<0.05) since the adjustment of national parks. Based on the policy approach reflecting regional characteristics, natural resources are kept and enhanced for the sustainable use and management of national parks. And a consensus on the national park policy of national park should be developed between local residents and voluntary participation and public awareness should be attracted from them, based on the construction and the supplement of the infrastructure.

고양이의 만성 췌장염 치료 증례

김현아,윤기영,박정호,이왕희,송근호,박성준 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2011 動物醫科學硏究誌 Vol.18 No.1

A 5-year-old Russian blue cat was referred to the Veterinary Medical Teaching Hospital of Chungnam National University with the history of anorexia, lethargy, weight loss(6.5 to 4.8 kg) 2 weeks ago. Abdominal ultrasonography showed the following : increase of echogenicity of liver, irregular shape of gall bladder, hyperechoic region around duodenum. Pancreatitis, cholangitis, hepatic lipidosis was diagnosed by the ultrasonography. Anti- inflammatory therapy, antibiotic therapy was administered. Abdominal ultrasonography is a efficient available diagnostic tool but serum fPL test reveals the highest sensitivity and specifity result for feline pancreatitis. SNAP fPL test is newly released that can check pancreatitis within 10 minutes by same princple of Spec fPL test used in Gastrointestinal Laboratory of Texas A & M Univerrsity.

서울시내 대학 내 보건의료시설의 현황

박현아 韓國學校保健學會 2000 韓國學校保健學會誌 Vol.13 No.2

Background : One-quarter of Koreans are either students or school employees. Therefore, school health programs for them have high levels of cost-benefit. School health programs, though, are focused on services such as vaccination and physical examination according to administrational regulations without systemic planning. Futhermore, college health programs run autonomously, not under the supervision of the Ministry of Education. It is my intention to analyse the current status of college school health service centers and use the basic data so generated to model how they might operate at an optimal level of efficiency. Methods : I intended to investigate all 29 colleges in Seoul except some specialized colleges such as theological schools in the two-month period of August and September, 1999. I used the telephone interview method to ask questions relating to personal composition, medical equipment in use, annual expenditure and the provision of school health services. School health services were composed of three items; health services, health education and a healthy school environment. Results : 27 college health service centers were surveyed. The median number of medical personal in each center was 2, the range was 1-31. 7 centers(25.9%) have only nurses with no doctors. Annual expenditures of 11 centers(50.1%) was less than 10 million won, 19 centers(70.4%) were maintained by support from their college. Thirteen centers(48.1%) provided doctor's examinations, 6 centers(22.2%) provided dental care services, laboratory services were provided by seven centers(25.9%). Some 81.5% of the centers had vaccination programs and 44.5% had health education programs. There was no school environment program except insecticide provisions. College health service centers with school doctors differed from centers without school doctors in terms of medical equipment range, annual expenditures and annual case loads. Conclusion : The structure and function of college health service centers in Seoul are diverse. However, no center has a well-organized school health plan.

개 비장 질환의 미세세침흡입술을 통한 세포병리학 검사 4례

김현아,윤기영,최호정,정성목,이영원,조성환,박성준 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2011 動物醫科學硏究誌 Vol.18 No.1

To evaluate the accuracy and diagnostic significance of cytopathological diagnosis of canine splenic disorders, cytopathological examination throgh fine-needle aspiration(FNA) referred to Veterinary Medical Teaching Hospital of Chungnam National University were retrospectived. Splenic neoplasm, hemolymphatic neoplasm, hyperplasia, extramedullary hematopoiesis and splenitis were diagnosed canine splenic disorders through cytopathological examination. The patient should be identified what coagulation profile are normal before aspiration, because complication such as hemorrhage could happen. The 4 cases were identified symmetric, asymmetric splenomegaly on abdominal ultrasonography and then were performed ultrasound-guided FNA. The 4 cases were diagnosed hemangiosarcoma(2), hyperplasia(1) and extramedullary hematopoiesis(1) by clinical signs, hematological examination, and cytopathological examination. Cytopathological examination through FNA was cost-effective, no gneral anesthesia, no complication, and non-invasive method of diagnosis for canine splenic disorders.

위탁급식업체 선정에 영향을 미치는 요인에 대한 개발영업사원의 인식도 조사

김현아,양일선,이보숙,박진영 대한영양사협회 2004 대한영양사협회 학술지 Vol.10 No.2

The purpose of this study was to investigate the importance level on factors affecting the foodservice management contract perceived by the salespersons in the foodservice company. To get the data on the perceived importance level on the affecting the foodservice management contract, the questionnaires were developed by the delphi technique and modified by the pilot test. The questionnaires consisted of 4 categories and 19 items on the factors affecting the foodservice management contract and the importance level on the factors were measured by 5-likert scale. From March 12 to April 13 in 2003, the self-administrative questionnaires were mailed to the 60 salespersons. The questionnaires were responded from the 48 salespersons(respondent rate : 77%). As a result, the salesperson' perceived importance level of 「the sales ability」 was higher than those of the other categories in the private contract and the salesperson' perceived importance level of 「the evaluation of the foodservice company」 was higher than those of the other categories in the competitive bid. The importance of the 「the evaluation of the foodservice company」 between the private contract and the competitive bid was significantly different(p<.05), and the importance of 「the sales ability」 of the competitive bid was significantly higher than that of the private contract(p<.001).

식중독 세균 5속을 검출하기 위한 4개 주요 REP-PCR 반응성분의 최적화

서현아,박성희,송대식,김근성 한국산업식품공학회 2003 산업 식품공학 Vol.7 No.4

반복성 염기서열을 응용한 PCR법인 REP-PCR을 사용하여 식중독 미생물의 분리와 동정을 위한 연구에 적용하기 위하여 주요 식중독 유발세균 5속(genus), 6종의 균주를 사용하여 실험을 실시하였다. PCR 구성성분인 MgCl₂, dNTPs, REP sequence primer, 주형 DNA의 농도를 최적화하기 위한 연구 결과, MgCl₂의 농도가 2.0mN과 2.5mN일 때, 균주들의 fingerprinting pattern의 변화가 없거나 적은 것으로 관찰되어, 모든 속의 균주를 단일조건으로 분리?동정하기 위해서 MgCl₂의 농도는 2.5mN이 최적인 것으로 결론을 내렸다. dNTPs는 50μM의 농도를 적용하였을 때부터 전체 fingertprinting pattern범위의 주 단편들을 나타내는 균주들도 있었으나 총 단편의 수가 완벽하게 나타나지는 않았고, 200μM의 적용시점에서 6종의 균주 모두 단편의 수나 강도의 변화가 관찰되지 않았기 때문에 fingerprinting pattern의 파악을 위한 목적에는 200μM의 dNTPs 농도만으로도 충분하였다. REP primer는 적용한 농도가 증가함에 따라 단편의 수와 강도가 증가하였으며 Vibrio를 제외한 5종의 균주가 2.0μM의 primer를 적용했을 때 고유한 fingerprinting pattern을 나타냈다. 주형 DNA의 양을 변화시켜 적용하였을 때 DNA 양의 비율이 2배에서 5배까지 증가하여도 초기 미량 적용 시 생성되었던 fingerprinting pattern은 많은 차이를 보이지는 않았다. 그러나 적용되는 DNA 양에 따라 단편들의 강도와 수가 약간씩 점증하는 양상을 보였다. This experiment was conducted to optimize REP(repetitve extragenic palindromic sequence)-PCR reaction conditions in order to simultaneously differentiate the five different foodborne pathogenic bacterial genera of Escherichia, Salmonella, Shigella, Vibrio and Listeria. The four major PCR cocktail components of MgCl₂, dNTPs, primers and teplate DNA were considered and their optimum concentrations were determined using six reference strains. the optimized condentration of MgCl₂ was determined to be 2.5 mM in order to obtatin a consistent fingerprinting pattern. The similar fingerprinting pattern was obtatined when REP primers and dNTPs were added up to the concentration of 2μM and 200μM, respectively. As for template DNA, the numbers of PCR fragments were not mostly affected, but their intensities were slightly increased as the concentrations of the DNA were increased from 2 times to 5 times.

升麻葛根湯 및 升麻葛根湯合四物湯의 抗 Histamine 效果에 關한 硏究

金賢兒,鄭智天,朴宣東 동국대학교 한의학연구소 1994 東國韓醫學硏究所論文集 Vol.3 No.-

In order to find out the effect of Anti-Histamine of the Sungmagalkuntang and Sungmagalkuntang-plus-samultang I've investigated the effect of the time to death, the peripheral vascular Peaneability, the amount of secretion of Gastric Juice, the total Acidity of Gastric Juice, the contraction of Intestine Tube by Histarnine and the effect of the Skin-Reaction by the decrease of DNCB. These results are following 1. The effect of Mice s rime to death by Histamine was that the injection group of sungmagalkuiigtang and the injection group of sungmagalkungtang-plus-samultang both have had the effect of the extention uf the time lo death, which indicated a statistical significance in comparison with comparative group. 2. The effect of the increased vascular permeability by Histamine was that the injection group of sungmagalkuntang and the injection group of sungmagalkuntang-plus-samultang both have had the effect of the restraint of the increased vascular permeability, which indicated a statistical significance in comparison with comparative group. 3. The effect of the the amount of the secretion of Gastric Juice by Histamine stimulus was tirat the injection group of sungmagalkuntang-plus-samultang only has had the effect of restraint of the increased of the secretion of Gastric Juice, which indicated a statistical significance in comparison with comparative group. 4. The effect of the chan,ge of total Acidity of Gastric Juice by Histamine's stimulus was that the injection group of sungmagalkuntang-plus-samultang only has had the effect of the restraint of the increase of tolal Acidity of Gastric Juice,, which indicated a statistical significance in comparison with comparative group. 5. The effect of the contraction of Intestine Tube by Histamine was that sungmagalkuntang and sungmagalkuntang-plus-samultang both have had the effect of the contraction and the restraint According to the increase of the density, the effect of the restraint was more clear. But the difference of the effects between two substances had little. 6. The effect of the reaction of skin by the decrease of DNCB was that the injection group of sungmagalkuntang and the injection group of sungmagalkuntang-plus-samultang have had the decreased effect of the Skin-Reaction in comparison with comparative group. sungmagalkun-tang-plus-samultang aws more effective. In these results shown above, both sungmagalkuntang and sungmagalkuntang-plus-samultang was shown as more effective one

암치료에 있어서 체질과 항암효과에 관한 한의학적 연구

성현제,신현규,박갑주,강봉주,은영아,김은해,정세영 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

As one of the chemical and experimental study on cancer treatment in Korea oriental medical hospital. Furthermore, we carried out the cytotoxicity test of extracts of herbal medicine. The results are as follows : About Clinical Aspects of the Oriental Medical Cancer Treatment - (1) Clinical experience is that 420 Oriental medical hospital were surveyed. Number of cancer patient treated by one oriental medical doctor was under 10(about 60%) or from 10 to 20(about 30%). (2) Diagnostic Procedure is that more than 80% of cancer patients were diagnosed by medical doctor of western hospital. (3) Therapeutic Method is that combination of oriental and western medical treatment was used in oriental medical hospital. (4) Relationship between cancer and constitutions is that to the relationship between cancer and constitutions, about 75% responded and most were considered to be Um. About Experimental Study - to evaluate the direct cytotoxicity of hot water and methanol extracts of 34 oriental medical presciptions on stomach cancer cell, SRB assay was carried out. (1) The IC50 value of water extract of Samsoum was 19㎍/㎖ and that of methanol extract was 15㎍/㎖, so that we confirmed Samsoum has a strong direct cytotoxicity. (2) The IC50 values of water extract of Coptidis Rhizoma, methanol extract of Caesalpiniae Lignum and Draba Semen were 10㎍/㎖, 19㎍/㎖ and 12.5㎍/㎖ respectively, so that we confirmed Coptidis Rhizoma, Caesalpiniae Lignum and Draba Semen have a strong direct cytototoxicity. (3) The IC50 value of combinatory prescription of Rhei Rhizoma, Forsythie Fructus and Caesalpiniae Lignum was about 40㎍/㎖.

현대 패션에 나타난 그린(GREEN)디자인

이현아;이연희;박재옥 한양대학교 2005 韓國 生活 科學 硏究 Vol.25 No.2

The purpose of this study was to analyze the characteristics of green design in the 1990s based on ecological aesthetics and identify the trend toward eco-design in different times. It was based on our study of literatures and cases along with analysis of contents. To establish the concept of eco-design, the case study was conducted on relevant literatures and photographs, and in terms of cases related to fashion, the study of all design trends occurred in the past analyzed the characteristics of design elements between 1990 and 1999. As the result, trend toward green design could be split into naturalism, minimalism and recycle. The first two trends share the core concept of reduce. As the trend pursued minimalism, at the production stage, it had a simple form and was designed using materials that can minimize the damage to the environment. This kind of materials had natural colors such as ecru. Addition ally, in the recycle trend, recycling fashion that came from the need to conserve resources and protect environment caught on with the reuse of old materials and various methods of expression through diversity of materials. Almost anything could be used; fabrics, paper, recycled plastic, and everyday products. And you can see that fashion had played an active role in recycling resources using various methods like patchwork, collage, fraying, fringe, and layering

제대혈 채취 후 유로키나제의 첨가가 제대혈 적혈구 제거시 조혈모세포의 수득률에 미치는 영향

이영호,박현우,이영아,한훈,김경희,한진영 대한조혈모세포이식학회 2000 대한조혈모세포이식학회지 Vol.5 No.1

목적:제대혈 채취 후 24시간이 경과하여 적혈구를 제거하는 경우에 제대혈내의 응고계 및 섬유소 융해계의 변화로 인하여 clumping이나 미세혈전 등이 생겨 유핵세포나 CD34+ 세포의 수득률이 감소될 수 있다. 따라서 비록 항응고제가 처리된 제대혈이라 할지라도 제대혈 분리 전에 urokinase를 첨가하면 조혈모세포의 수득률을 높일 수 있을 것으로 생각된다. 그러나 현재까지 이에 대한 연구가 전혀 없는 상태이므로, 본 연구에서는 시간이 경과된 제대혈에 urokinase를 첨가하는 것이 효과가 있는지, 있다면 얼마의 용량이 적절한지 알아보기로 하였다. 방법:1) 채취한 후 48시간 경과된 제대혈에 대한 분석: 15례의 제대혈을 채취하여 채취 직후와 48시간 후에 10% pentastarch로 적혈구를 분리한 다음, 총유핵세포수와 CD34+ 세포수를 측정하여 수득률을 비교하였다. 48시간이 경과한 검체에 대하여 urokinase는 제대혈 분리 30분 전에 첨가하였으며, urokinase를 첨가하지 않은 군과 urokinase 5,000 IU/mL, 10,000 IU/mL, 50,000 IU/mL을 첨가한 네군으로 나누어 각각의 수득률을 비교 분석하였다. 2) 채취한 후 24시간이내의 제대혈에 대한 분석: 12례의 제대혈을 채취한 직후에 적혈구를 분리하여 총유핵세포수와 CD34+ 세포수, CFU-GM 집락수를 측정하였다. 동일한 검체를 6시간, 12시간, 24시간 동안 실온에 방치한 후 상기 실험 결과 가장 효과적인 용량의 urokinase를 첨가한 군과 첨가하지 않은 군으로 나누어 각각 적혈구를 분리한 다음, 총유핵세포수와 CD34+ 세포수, CFU-GM 집락수를 측정 비교하였다. 결과:1) 제대혈 채취 48시간 후에 urokinase를 첨가하지 않은 경우와 urokinase 5,000 IU/mL를 첨가한 경우 총유핵세포수의 차이가 없었지만, urokinase 10,000 IU/mL, 50,000 IU/mL를 첨가한 경우에는 urokinase를 첨가하지 않은 경우에 비하여 총유핵세포수의 의미있는 증가를 보였다(P=0.0024, P=0.0009). 2) 제대혈 채취 48시간 후에 urokinase 5,000 IU/mL이나 50,000 IU/mL를 첨가한 경우에는 urokinase를 첨가하지 않은 경우에 비하여 CD34+ 세포수의 차이가 없었지만, urokinase 10,000 IU/mL를 첨가한 경우에는 urokinase를 첨가하지 않은 경우에 비하여 CD34+ 세포수의 의미있는 증가를 보였다(P=0.0402). 3) Urokinase 10,000 IU/mL를 첨가하였던 군이나 첨가하지 않았던 군 모두에서 제대혈 채취 즉시 적혈구를 분리하였던 경우에 비하여 6시간, 12시간, 24시간이 경과할수록 총유핵세포수, CD34+ 세포수, CFU-GM 집락수가 약간씩 감소하였으나 시간경과에 따른 통계적 차이는 없었다. 또한 제대혈 채취 24시간 이내에는 각 시간대별로 urokinase 10,000 IU/mL 첨가 유무에 따른 총유핵세포수, CD34+ 세포수, CFU-GM 집락수의 통계적 차이를 나타내지 않았다. 결론:채혈한지 48시간 경과된 제대혈을 냉동 보관해야 하는 경우에는 urokinase 10,000 IU/mL를 첨가하고 30분 후에 적혈구를 분리하는 것이 유핵세포와 CD34+ 세포의 수득률을 높일 수 있는 방법이며, 24시간 이내에 적혈구를 분리하는 경우에는 urokinase를 첨가할 필요가 없을 것으로 생각된다. Background:We assessed whether the urokinase could increase the yield of progenitor cells during processing in elapsed, even anticoagulated, cord blood after collection, and also determined the optimal dosage of urokinase. Methods:Twenty-seven cord blood samples were collected with ACD-coated syringes from umbilical cord vein after full-term vaginal delivery, and red cells were depleted with 10% pentastarch. We assessed the effect and optimal dosage of urokinase by comparing total nucleated cell (TNC) counts and CD34+ cell counts between fresh and 48 hour-elapsed cord bloods. The urokinase was administered to the 48 hour-elapsed cord bloods 30 minutes before separation as the dose of 0 IU/mL, 5,000 IU/mL, 10,000 IU/mL and 50,000 IU/mL, respectively. Thereafter, by using the most effective dosage of urokinase, we also assessed the effect of urokinase in the 6, 12 and 24 hour-elapsed cord bloods. Results:The TNC counts after separation in 48 hour-elapsed cord bloods were significantly higher in 10,000 IU/mL and 50,000 IU/mL of urokinase treated samples than untreated and 5,000 IU/mL treated samples. The CD34+ cell counts were significantly higher in 10,000 IU/mL of urokinase treated samples than untreated and 5,000 or 50,000 IU/mL treated samples. In 6, 12 and 24 hour-elapsed cord bloods, however, there were no significant differences of TNC count, CD34+ cell count and CFU-GM count between 10,000 IU/mL of urokinase treated samples and untreated samples . Conclusion: The addition of 10,000 IU/mL of urokinase before separation of 48 hour-elapsed, even anticoagulated, cord bloods could increase the yield of progenitor cells. However, there are no advantage of urokinase for processing of cord bloods not elapsed 24 hours after collection.