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이왕희,오일남,최승현,박종태 한국식품과학회 2021 Food Science and Biotechnology Vol.30 No.10
Food authenticity is one of the largest concernsin recent days. As kimchi has been a global food, its productionorigin has been important issue, particularly due tothe large import from China. Among the potential methods,electronic nose which can measure volatile compounds infoods is considered to be a powerful device for identifyingcountry of production. This study is to classify 69 kinds ofkimchi produced in South Korea (39) and China (30) byanalyzing volatile compounds in kimchi using electronicnose-mass spectrometry. Two widely used multivariateanalyses, discriminant function analysis and principalcomponent analysis, were used. Results showed that bothmultivariate analyses can completely separate Korean andChinese kimchi using 10 kinds of molecular weightsamong 10–160 amu. The results indicate that the volatilecompounds in kimchi are a suitable target to determine thegeographical origin of kimchi.
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Mechanistic Modeling in Biosystem Engineering: A Review on Mechanistic Models of Calcium Metabolism
이왕희,조병관 한국산업식품공학회 2013 산업 식품공학 Vol.17 No.1
Calcium is an essential nutrient generally obtained from dietary foods. However, its metabolism is a complex biological network requiring an effective analytical tool. In this review, a few notable calcium mechanistic models are assessed, emphasizing the power of mechanistic modeling in analyzing biological systems because no publication has reviewed them in a single place. Reviewed models are categorized by target systems: calcium absorption, calcium excretion, bone turnover, and whole calcium homeostasis. In the calcium absorption model, two transport systems carrying calcium from intestine to blood have been mechanistically described. Since urine calcium excretion is poorly understood, its model has not been explicitly developed although it is modeled as a part of the whole calcium metabolic system. Cell-based bone turnover model has been conceived to describe the mechanism under bone cell regulation. Finally, whole calcium metabolism has been a target to explain the metabolic control of calcium homeostasis that is linked to the endocrine system. Reviewed models focus on explaining how the calcium metabolic system behaves in response to conditional perturbations by involving effective factors such as endocrine system. We expect that not only will this study provide comprehensive information for future studies in calcium metabolism, but also that it will suggest what the concepts of biosystem modeling are and how they can be used for assessing the target system.
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Efficacy of Modified Rush Allergen-Specific Immunotherapy on Canine Atopic Dermatitis
이왕희,박성준 韓國臨床獸醫學會 2017 한국임상수의학회지 Vol.34 No.4
Modified rush ASIT protocol has been performed to identify the ideal schedule that allows the dose considered effective to be reached in the shortest possible time with the fewest adverse effects. Ten atopic dogs of this study includes fulfillment of Favrot’s criteria. Offending allergens were identified by the use of IDST. During the induction period, the dogs were received a total of 15 injections. Ten injections were administrated every 30 minutes in a day with gradually increasing amounts and concentrations of allergens, and the last 5 injections were administered every 3 days. Disease severity was quantified by using the canine atopic dermatitis extent and severity index (CADESI). During induction period, reduction rate from baseline scores varied between 1% and 67% and the improvement of ≥ 50% was recorded after induction period of therapy for CADESI-03 score in 6 of the 10 dogs. This study of ten dogs with atopic dermatitis provide evidence for the efficacy and safety of modified rush ASIT for clinical improvement.
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이왕희,최승현,오일남,심준용,이광식,안길환,박종태 한국식품과학회 2017 Food Science and Biotechnology Vol.26 No.3
An electronic nose-mass spectrometry (EN-MS) that profiles volatile compounds is a candidate device for identifying the geographic origin of cultivation of agricultural products when an adequate algorithm is derived. The objectives of this study were to apply two types of multivariate analysis, discriminant function analysis (DFA) and principal component analysis (PCA), to the volatile compounds detected by an EN-MS for the geographic classification of Chinese cabbage cultivated in Korea (42 samples) or in China (29 samples). DFA showed that Chinese cabbage from Korea were completely separable from those originating in China with 12 volatile compounds among the 151 detected. PCA revealed that Chinese cabbage data fell into two completely separable origins of Korea and China. This is the first study involving EN-MS data of volatile compounds with multivariate statistics to discriminate the geographical origin of Chinese cabbage, with further applications for other agricultural products.
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Mathematical Modeling and Optimization of Active Calcium Absorption in Human Body
이왕희,김도균 한국농업기계학회 2019 바이오시스템공학 Vol.44 No.3
Purpose Active calcium (Ca) absorption is the first process in Ca metabolism, which regulates the whole Ca absorption. There are a few notable models which depict the mechanism of active Ca absorption; it is still required to update it for human metabolism and optimal conditions to propose an adequate amount of absorbed Ca. In this study, we were to develop a mathematical model describing the active Ca absorption and optimized the active Ca absorption. Methods The developed model explained the active Ca absorption with 3 steps: Ca entry by electrochemical diffusion, intracellular Ca diffusion in cell by facilitated diffusion, and Ca extrusion in Ca pump by Michaelis–Menten equation. Results Model simulation was validated by showing similar responses with a previous model; then, the model was applied to simulate the active Ca absorption with parameters estimated based on human studies. In addition, the simulated Ca absorption was optimized as a function of Ca load in intestine and regulatory molecule, i.e., Ca-binding protein. Conclusions This study provides a mechanistic point of view in human active Ca absorption and is expected to be a useful tool for analyzing the whole Ca metabolism and for designing Ca-related clinical experiment.
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이왕희,조병관 한국농업기계학회 2012 바이오시스템공학 Vol.37 No.4
Purpose: This review aims at introducing 3 modeling approaches classified into 3 categories based on the purpose (estimation or prediction), structure (linear or non-linear) and phase (steady-state or dynamic-state); 1) statistical approaches, 2) kinetic modeling and 3) mechanistic modeling. We hope that this review can be a useful guide in the model-based approach of calcium metabolism as well as illustrates an application of engineering tools in studying biosystems. Background: The meaning of biosystems has been expanded, including agricultural/food system as well as biological systems like genes, cells and metabolisms. This expansion has required a useful tool for assessing the biosystems and modeling has arisen as a method that satisfies the current inquiry. To suit for the flow of the era, examining the system which is a little bit far from the traditional biosystems may be interesting issue, which can enlarge our insights and provide new ideas for prospective biosystem-researches. Herein, calcium metabolic models reviewed as an example of application of modeling approaches into the biosystems. Review: Calcium is an essential nutrient widely involved in animal and human metabolism including bone mineralization and signaling pathways. For this reason, the calcium metabolic system has been studied in various research fields of academia and industries. To study calcium metabolism, model-based system analyses have been utilized according to the purpose, subject characteristics, metabolic sites of interest, and experimental design. Either individual metabolic pathways or a whole homeostasis has been modeled in a number of studies.
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