Oncoplastic surgery of malignant melanoma of breast

은영아,김진영,박호용,정진향 대한외과학회 2009 대한외과학회 학술대회 초록집 Vol.2009 No.11

인간 대뇌피질에서 Mu-Opioid 수용체의 아류에 관한 연구

은영아,김완호,김기원 의과학연구소 1998 全北醫大論文集 Vol.22 No.2

인간 대뇌피질 세포막표본에서 μ-수용체 subtypes의 존재를 확인하고, 약물 결합특성을 파악하기 위하여 opioid μ₁_과 μ₂_ 수용체에 각각 높은 선택성과 친화력을 갖는 [³H]DADL(μ₁:10 n^M DPDPE 존재하)과 [³H]DAMGO ( μ₂: 5nM DS-LET 존재하)의 수용체결합에 대한 agonists와 antagonists의 억제효능을 검토하여 다음과 같은 결과를 얻었다. 1. [³H]DADLE 결합은 opioid μ-수용체에 agonist 작용을 갖는 TAPS, DAMGO, fentanyl, morphine과 DSLET에 의해 억제되었으며, 그 친화력의 정도는 TAPS>DAMGOA>morphine>fentanyl>DSLET의 순이었다. 그리고 선택적 opioid μ₁-antagonist인 CTOP은 비선택적 antagonist인 naloxone 보다 친화력이 강하였다. β-Endorpohin과 dynorphin A는 공히 이 수용체에 친화력을 보였으나, β-endorpohin의 친화력이 현저히 강하였다. 2. 인간 대뇌피질 세포막표본에서 DADLE (5nM) 존재시 [³H]DAMGO는 DADLE없을 때보다 낮은 친화력을 보이는 한 종류의 결합부위를 표지하였다. 이 결합부위는 μ₁_수용체의 경우와 같이 TAPS, DAMGO, fentanyl, morphine과 DSLET, CTOP, naloxone, β-Endorpohin 그리고 dynorphin A에 친화력을 보였으나, 그 친화력은 정도는 μ₁_수용체와 달리 DAMGO>morphine=fentanyl=TAPS>1/DADLE>DSLET의 순이었다. β-Endorpohin 의 경우에는 μ₂_ 수용체에 대한 친화력이 μ₁_수용체 보다 컸다. 이상의 실험성적은 인간 대뇌피질의 opioid μ₁_과 μ₂_수용체로 구별되며, 이 수용체들은 각각 상이한 약물결합특성을 갖음을 시사한다.

흰쥐 대뇌피질 절편에서 허혈에 의한 Norepinephrine 유리에 있어서 Nitric Oxide의 영향

은영아,김동찬,조규박,김기원,Eun, Young-Ah,Kim, Dong-Chan,Cho, Kyu-Park,Kim, Kee-Won 대한약리학회 1997 The Korean Journal of Physiology & Pharmacology Vol.1 No.6

It has been generally accepted that glutamate mediates the ischemic brain damage, excitotoxicity, and induces release of neurotransmitters, including norepinephrine(NE), in ischemic milieu. In the present study, the role of nitric oxide(NO) in the ischemia-induced $[^3H]norepinephrine([^3H]NE)$ release from cortex slices of the rat was examined. Ischemia, deprivation of oxygen and glucose from $Mg^{2+}-free$ artificial cerebrospinal fluid, induced significant release of $[^3H]NE$ from cortex slices. This ischemia-induced $[^3H]NE$ release was significantly attenuated by glutamatergic neurotransmission modifiers. $N^G-nitro-L-arginine$ methyl ester(L-NAME), $N^G-monomethyl-L-arginine$ (L-NMMA) or 7-nitroindazole, nitric oxide synthase inhibitors attenuated the ischemia-evoked $[^3H]NE$ release. Hemoglobin, a NO chelator, and 5, 5- dimethyl-L-pyrroline-N-oxide(DMPO), an electron spin trap, inhibited $[^3H]NE$ release dose-dependently. Ischemia-evoked $[^3H]NE$ release was inhibited by methylene blue, a soluble guanylate cyclase inhibitor, and potentiated by 8-bromo-cGMP, a cell permeable cGMP analog, zaprinast, a cGMP phosphodiesterase inhibitor, and S-nitroso-N-acetylpenicillamine (SNAP), a nitric oxide generator. These results suggest that the ischemia-evoked $[^3H]NE$ release is mediated by NMDA receptors, and activation of NO system is involved.

비신생물성 질환에 대한 췌십이지장 절제술의 안정성 및 수술 후 삶의 질에 대한 평가

은영아(Young A Eun),김상걸(Sang Geol Kim),윤혁진(Hyuk Jin Yun),김종렬(Jong Yeol Kim),김갑철(Gab Chul Kim),염현규(Hyun Kyu Ryeom),김성희(Sung Hi Kim),황윤진(Yun Jin Hwang),윤영국(Young Kook Yun) 대한외과학회 2007 Annals of Surgical Treatment and Research(ASRT) Vol.73 No.1

Purpose: Pancreaticoduodenectomy for mass-like lesions that are suspicious of malignancy sometimes reveals only nonneoplastic disease, especially in those cases where adequate tissues for biopsy are unavailable. In this study, we evaluated the outcomes and quality of life (QOL) after pancreaticoduodenectomy for treating nonneoplastic disease. Methods: The clinical data of 28 patients who underwent pancreaticoduodenectomy for nonneoplatic disease and trauma from Jan. 1992 to Feb. 2006 were reviewed retrospectively. The QOL was evaluated using the FACT-Hep questionnaire. The patients who underwent laparoscopic cholecystectomy for benign gallbladder disease were utilized as the control group. Results: 13.8% (28/203) of all the pancreaticoduodenectomized patients had nonneoplatic disease and trauma. Male patients were predominant (25/28) and all the trauma patients were male. The mean age was 48 (23∼72) years old. The indications for surgery included lesions suspicious for malignancy (16 cases), pancreaticoduodenal artery aneurysm (1 case), and pancreatoduodenal injury (11 cases). The histologic findings of the nonneoplastic lesions revealed benign inflammation of the bile duct (6 cases), chronic pancreatitis (8 cases), pancreatic pseudocyst (1 case), and fibrosis of the Ampulla of Vater (1 case). No surgical mortality occurred. However, the trauma patients group had higher morbidity (72.7% Vs 23.5%, respectively, P=0.01) and a longer hospital stay (68.0 days Vs 32.6 days, respectively, P=0.02) after surgery compared to the nonneoplastic disease patient group. The QOL of the patient who underwent pancreaticoduodenectomy for nonneoplatic disease was not different from that of the control group. Conclusion: Since pancreaticoduodenectomy for nonneoplastic disease was safe and the QOL of the patients was acceptable, it should be performed more often when malignancies can not be excluded from the differential diagnosis.

한약처방제의 인체 위암 세포주에 대한 세포독성 효과에 관한 연구

김은해,은영아,강봉주,성현제,박갑주,Kim, Eun-Hae,Eun, Young-Ah,Kang, Bong-Joo,Sung, Hyun-Jae,Park, Kap-Joo 한국생약학회 1997 생약학회지 Vol.28 No.4

ln order to search for antigastric cancer agents from Korean traditional prescriptions. We selected 41 traditional prescriptions, based on a review of the Korean traditional medicine books. Both boiling water and methanol extracts were tested, by means of the Sulforhodamine B (SRB) protein assay. Six of the 41 water extracts; #3, #34, #35, #38, #40, #41 showed efficacy against gastric cancer cell (AGS: Human gastric carcinoma, ATCC HTB 103). #3 inhibited 50% cancer cell growth1 at the concentration of $152\;{\mu}g/ml$, #34, #35, #38, #40 and #41 inhibited 50% cancer cell growth at the concentration of $145\;{\mu}g/ml$, $129\;{\mu}g/ml$, $173\;{\mu}g/ml$, $10\;{\mu}g/ml$ and $19\;{\mu}g/ml$ respectively. Ten of the 41 methanol extracts; #1, #3, #32, #33, #35, #36, #37, #38, #41 were active. #1 inhibited 50% cancer cell growth at the concentration of $206\;{\mu}g/ml$, #3, #32, #33, #35, #36, #37, 738, #40, #41 inhibited 50% cancer cell growth at the concentration of $133\;{\mu}g/ml$, $159\;{\mu}g/ml$, $199\;{\mu}g/ml$, $147\;{\mu}g/ml$, $113\;{\mu}g/ml$, $187\;{\mu}g/ml$, $130\;{\mu}g/ml$, $9\;{\mu}g/ml$, $15\;{\mu}g/ml$ respectively. Prescription #3, #35, #38, #40, #41 were also interesting because both methanol and water extracts were active.

암 예방 및 한방 치료에 관한 연구

성현제,윤유식,은영아,김연옥,김희수,박윤신,최선미 한국한의학연구원 1999 한국한의학연구원 연구보고서 Vol.- No.-

대장균에서 많은 수의 플라스미드에 존재하는 rnpB 유전자 전사의 성장기의 따른 조절 작용

정영환,길민찬,전은순,은영아,이영훈,박충웅 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.16 No.-

The Escherichia coli rnpB gene encodes M1 RNA, the catalytic component of RNase P which is a processing enzyme for tRNA maturation. Biosynthesis of M1 RNA is known to be stringently regulated. The rnpB transcription under different growth phases of E. coli was examined using the truncated rnpB in the multicopy plasmids which could generate the metabolically unstable M1 RNA transcript. Biosynthesis of this RNA was directly analyzed during the transition from the exponential phase of growth to the stationary phase. During the transition the rnpB transcription became repressed. The discriminator motif CGCC adjacent to the transcription start-point of the rnpB gene was altered to the TATT using site-directed mutagenesis. The modified promter containing the mutated discriminator was at least partially rescued from this growth phase-dependent repression of the rnpB transcription. The results clearly show that the discriminator motif play a key role in control of the rnpB transcription under different growth phases.

암치료에 있어서 체질과 항암효과에 관한 한의학적 연구

성현제,신현규,박갑주,강봉주,은영아,김은해,정세영 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

As one of the chemical and experimental study on cancer treatment in Korea oriental medical hospital. Furthermore, we carried out the cytotoxicity test of extracts of herbal medicine. The results are as follows : About Clinical Aspects of the Oriental Medical Cancer Treatment - (1) Clinical experience is that 420 Oriental medical hospital were surveyed. Number of cancer patient treated by one oriental medical doctor was under 10(about 60%) or from 10 to 20(about 30%). (2) Diagnostic Procedure is that more than 80% of cancer patients were diagnosed by medical doctor of western hospital. (3) Therapeutic Method is that combination of oriental and western medical treatment was used in oriental medical hospital. (4) Relationship between cancer and constitutions is that to the relationship between cancer and constitutions, about 75% responded and most were considered to be Um. About Experimental Study - to evaluate the direct cytotoxicity of hot water and methanol extracts of 34 oriental medical presciptions on stomach cancer cell, SRB assay was carried out. (1) The IC50 value of water extract of Samsoum was 19㎍/㎖ and that of methanol extract was 15㎍/㎖, so that we confirmed Samsoum has a strong direct cytotoxicity. (2) The IC50 values of water extract of Coptidis Rhizoma, methanol extract of Caesalpiniae Lignum and Draba Semen were 10㎍/㎖, 19㎍/㎖ and 12.5㎍/㎖ respectively, so that we confirmed Coptidis Rhizoma, Caesalpiniae Lignum and Draba Semen have a strong direct cytototoxicity. (3) The IC50 value of combinatory prescription of Rhei Rhizoma, Forsythie Fructus and Caesalpiniae Lignum was about 40㎍/㎖.

대장균에서 많은 수의 플라스미드에 존재하는 rnpB 유전자 전사의 성장기의 따른 조절 작용

이영훈,박충웅,전은순,정영환,길민찬,은영아 한국유전학회 1993 Genes & Genomics Vol.15 No.2

The Escherichia coli rnpB gene encodes M1 RNA, the catalytic component of RNase P which is a processing enzyme for tRNA maturation. Biosynthesis of M1 RNA is known to be stringently regulated. The rnpB transcription under different growth phases of E. coli was examined using the truncated rnpB in the multicopy plasmids which could generate the metabolically unstable M1 RNA transcript. Biosynthesis of this RNA was directly analyzed during the transition from the exponential phase of growth to the stationary phase. During the transition the rnpB transcription became repressed. The discriminator motif CGCC adjacent to the transcription start-point of the rnpB gene way altered to the TATT using site-directed mutagenesis. The modified promoter containing the mutated discriminator was at least partially rescued from this growth phase-dependent repression of the rnpB transcription. The results clearly show that the discriminator motif play a key role in control of the rnpB transcription under different growth phases.

조기 유방암으로 변형 근치적 절제술 후 단독국소재발한 환자의 예후

이승재(Seung Jae Lee),황승욱(Seung Oook Hwang),정진향(Jin Hyang Jung),박호용(Ho Yong Park),이준혁(Jun Hyuk Lee),은영아(Young A Eun) 대한외과학회 2009 Annals of Surgical Treatment and Research(ASRT) Vol.76 No.5

Purpose: Isolated local recurrence (ILR) after mastectomy is associated with increased risk of cancer-specific survival. This study evaluated the long-term survival and prognostic factors after ILR. Methods: Between January 1988 and December 2004, 1,169 patients with early breast cancer (stage Ⅰ or Ⅱ) were treated with modified radical mastectomy at Kyungpook hospital. Retrospectively, 40 patients with isolated local recurrence (ILR) were found by medical records and we investigated their clinicopathologic factors and conducted analyses of prognostic factors, calculated overall survival, and relapse-free survival. Results: The median follow-up times after initial operation and ILR were each 91 (range, 18∼219) months and 40 (range, 1∼205) months. There were 18 patients of 2nd recurrence and 15 expired by distant metastasis. The overall survivals at 5 years and 10 years after ILR were 65% and 49%. The relapse-free survivals at 5 years and 10 years after ILR were 54% and 29%. After 2nd recurrence, the mean survival time was 21 months and the 3-year overall survival rate was 21%. By univariate and multivariate analyses, the time interval between initial operation and ILR was only statistically significant independent prognostic factor of overall survival after ILR. The 10 year-overall survival rates of ≤24 months and >24 months to ILR were each 26% and 70%. There was no independent prognostic factor of relapse-free survival after ILR. Conclusion: For patients who developed isolated local recurrence after modified radical mastectomy for early breast cancer, shorter time interval to ILR is a poor prognostic factor of overall survival after ILR.