병원 가검물에서 분리된 주요세균의 항생제에대한 감수성

한영호,전효진,박은미,서장수,송도영,송경은,김재식,김중명 대한임상병리학회 1986 대한임상병리학회지 Vol.6 No.1

혼합 치열기 어린이의 치은 섬유종증

한효정,최병재,김진,김성오,손홍규 大韓小兒齒科學會 2004 大韓小兒齒科學會誌 Vol.31 No.4

치은 섬유종증은 유리 치은과 부착 치은이 점진적으로 증식되는 비염증성 질환이다. 대부분 가족력이 있으며 영구치 맹출과 함께 발병하지만 유치 맹출 시기에, 드물게 출생 시부터 나타나기도 한다. 본 6세 여아의 경우 태어날 때부터 치은의 일부가 증식되어 있었고 가족력 및 치은 증식을 유발하는 약물을 복용한 병력은 없었다. 구강 내 검진 시 섬유성 치은 증식이 관찰되어 절제 생검을 시행하였고 그 결과 치은 섬유종증으로 진단하였다. 치은 섬유종증의 치료는 치은 절제술과 철저한 구강 위생의 관리이다. 그러나 치료 후 재발성 때문에 치료 시기에 대해서는 논란 중이다. 일반적으로 영구치가 모두 맹출 한 이후에 치료할 것을 추천하지만 조기 치료는 구강의 기능 및 환아의 심미적, 심리적인 문제점을 개선시켜 주는 장점이 있다. Gingival fibromatosis is a non-inflammatory oral disease, characterized by slowly progress enlargement of the free and attached gingiva. Gingival fibromatosis may have familial tendency. Gingival enlargement usually begins with the eruption of the permanent dentition but can also develop with the eruption of the primary dentition. In this case, a 6-year-old female had gingival enlargement at birth. There was no familial, medical and pharmacologic history of gingival overgrowth. Treatment is gingivectomy with a rigorous program of oral hygiene. Recurrence of gingival fibromatosis may well be inevitable. Therefore there is no general aggrement as to the timing of surgical intervention. Generally the best time is when all the permanent teeth have erupted. However early intervention can improve oral function and esthetic and psychologic effect.

한국인 만성 간 질환 환자에서 G형 간염 바이러스 감염의 유병률에 대한 연구

이진호,이한주,윤정환,이효석,김정룡 大韓消化器病學會 1998 大韓消化器病學會誌 Vol.31 No.3

Agrobacterium tumefaciens를 이용한 bialaphos 저항성 형질전환 벼의 개발

이효연,이춘환,김호일,한원동,최지은,김진호,임용표 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-

비선택성 제초제인 bialaphos는 고등식물에 있어서 glutamine 합성을 억제하여 식물체를 고사시킨다. Acetyltransferase에 의해 encoding된 bialaphos 저항성 유전자는 세균 Streptomyces hygroscopicus SF1239로부터 cloning된 것을 사용하였다. Bialaphose 저항성 유전자를 Agrobacterium 감염법을 이용하여 국내에서 재배되는 벼(동진)에 도입한 결과 약 30%정도의 형질전환 식물체를 얻을 수 있었다. T_1세대의 17개체는 hygromycin과 bialaphos에 대한 저항성 유전형질이 3 : 1로 분리되었다. 또한 Southern 분석을 실시한 결과 wild type의 식물체에서는 Bar 유전자의 검출을 볼 수 없었으나 형질전환 식물체의 경우 Bar 유전자의 검출이 가능하였다. T_3 세대의 형질전환 식물체와 wild type의 식물체를 포장상태에서 비선택성 제초제인 바스타를 살포하고 3주 후에 관찰한 결과 형질전환 식물체는 외형적으로 아무런 피해를 받지 않고 정상적으로 생장하였으나, wild type의 식물체와 잡초는 모두 고사하였다. 이상의 결과를 종합해보면 hygromycin과 bialaphos 저항성 유전자는 Agrobacterium 감염법을 이용하여 단자엽 식물인 벼에 도입할 수 있다는 것을 보여준 것이며, 또한 bialaphos 저항성 유전자가 식물에 도입됨으로써 비선택성 제초제에 대한 저항성 식물을 개발 할 수 있다는 것을 보여 주었다. The bialaphos is a potent inhibitor of glutamine synthease in higher plants and is used as a non-selective herbicide. We have used the bialaphos resistant gene (Bar) encoding for an acetyltransferase isloated from Streptomyces hygroscopicus SF1293. Callus derived from mature seeds of rice (Oryza sativa L. cv. Dong Jin) were co-cultivated with Agrobacterium tumefacieus EHA101 carring a plasmid pGPTV-HB containing genes for hygromycin resistance (HygR) and Bar. Transgenic plants showing in vitro resistance to 50 mg/L hygromycin and 10 mg/L bialaphos were obtained by using a two-step selection/regeneration procedure. Transformation efficiency of rice was about 30% which was as high as reported in other dicotyledons. Progenies (T_1 generation) derived from primary transformant of 17 lines were segreagated with a 3 resistant:1 sensitive ratio in medium containing hygromycin and bialaphos. Stable integration of Bar gene into chromosomal DNA was proven by Southern blot analysis of genomic DNA isolated from T_2 progenies. Transgenic plants (T_3) grown in the field were resistant to bialaphos (Basta) at a dosage lethal to wild type plants.

실내·외 환경의 분진 중 중금속 농도 : 서울시 강서구·양천구 소재의 중학교를 중심으로 In Middle Schools in the Kangseoku and Yangchonku Areas, Seoul

배윤진,김규한,전효택,안주성 이화여자대학교 환경문제연구소 1998 이화환경연구 Vol.2 No.-

도시화 및 산업화에 따른 학교환경의 중금속 오염양상을 평가하기 위해 강서구·양천구에 위치한 31개 중학교의 교실, 특별실, 실외에서 강하분진 시료 총 93개를 채취하여 Cd, Co, Cr, Cu, Ni, Pb, Zn, Mn, Fe의 중금속 원소들의 함량을 측정하고, 다변량통계처리와 연속추출분석 및 전자현미분석을 실시하였으며 양천구의 한 학교에서 4개월간 채집한 부유분진 시료 2개를 전자현미분석하였다. 강서구·양천구 중학교들의 교실, 특별실, 실외 분진의 중금속 원소의 분산양상을 비교한 결과, Cd, Cu, Pb의 함량이 특별실>교실>실외 순으로 나타나 학교 실내의 폐쇄된 공간에서 Cd, Cu, Pb의 농집이 진행되고 있음을 보여주었다. 반면, Mn, Fe의 함량은 실외>특별실>교실 순으로 나타났다. 이러한 경향은 통계적으로 유의적이었다.(P<0.01). 그리고 대부분 학교의 실외시료에서 오염지수(Pollution index)가 1.0이상으로 조사되어 연구지역 학교들의 실외 중금속 오염이 매우 넓은 범위에 걸쳐 진행되고 있는 것으로 나타났다. 시료들에 대한 연속추출분석 결과, 교실, 특별실, 실외별로 중금속 원소들의 화학적 형태 분포상황이 유사하게 나타나 실내의 분진은 주로 외부에서 유입됨을 보여 주었다. 또한 Zn와 Cd의 교환성·수용성 형태가 우세하여 이동도와 생물흡수도가 가장 높을 것으로 나타났다. 전자현미분석에서도 시료채취 학교, 장소 및 용도등에 따른 중금속 존재형태의 차이는 나타나지 않았고 Pb, Zn, Cu 입자의 존재형태를 관찰한 결과, 실내외 중금속 오염이 과거 차량배기에 의한 오염영향과 공업활동에 의한 것으로 판단된다. Indoor and outdoor dry-deposited dust samples were collected from 31 middle school in the Kangseoku and Yangchonku areas in Seoul. Heavy metal concentrations were determined for the dusts from classroom, activity room and playground of each sampling sites. Suspended particulate samples were also investigated for certain periods during this study. Heavy metal dispersion patterns showed concentrations of Cd, Cu and Pb increasing in the order: playground, classroom and activityroom. Whereas Cd, Cu and Pb were highly concentrated in the closed spaces(e.g., activity room), Mn and Fe were high in the order of playground, activityroom and classroom. Most playground dusts exhibited the enhanced heavy metal pollution with a pollution index greater than 1.0. The chemical fractionations of Cd, Cu, Pb and Zn by sequential extraction were consistent between indoor and outdoor dust samples. Cadmium and Zn, mainly associated with exchangeable or water/acid soluble fraction, may have the highest mobility and bioavailability. There were no significant differences im morphological properties of the dust samples analyzed by electron probe microanalysis. It was suggested that outdoor dust may highly contribute to indoor contamination.

초등학교 학생들의 귀납연역적 추론 능력과 정신 용량 및 보석 오류와의 관계

김설한,정진우,김효남 한국교원대학교 과학교육연구소 1998 청람과학교육연구논총 Vol.8 No.1

The purpose of this study was to analyze the problem solving strategies of elementary school students and to find out correlations between the functional mental capacity, the perseveration error and the Creature Card Task solving ability. To study this purpose, four categories were selected through pilot test. The sample consisted of 231, the 4th grade students and the 5th grade students in Inchon, Korea and selected 32 students among them. Three instruments were used in this study, Creature Card Task, FIT(Figural Intersection Test) and WCST(Wisconsin Card Sorting Test). Researcher interviewed 32 students about Creature Card Task solving strategies and tests with FIT, WCST. Major findings of the study are as follows: 1. Creature Card Task solving strategies of the selected 4th & 5th grade students were different. Some students solved problems during individual interviews. 2. Creature Card Task solving abilities were significantly correlated with the functional mental capacity and the perseveration error.

복강경하 대장절제술을 시행 받는 환자에서 라모세트론과 온단세트론외 술 후 오심 및 구토 예방 효과 비교

김효중;어전영;어근무;어정한;엄세훈;조광래;김명훈 인제대학교 2011 仁濟醫學 Vol.32 No.-

Objectives : We evaluated the efficacy of ramosetron and ondansetron for preventing postoperative nausea and vomiting (PONV) in laparoscopic colectomy. Methods and Materials : Eighty patients who received laparoscopic colectomy were randomly divided into two groups: R group (ramosetron 0.1mg PO) and O group (ondansetron 4mg twice IV). Injection or oral medication was administered before the induction of anesthesia in each group. Injection was administered at the end of surgery in O group. General anesthesia was induced using propofol and rocuronium, and maintained with sevoflurane, remifentanil and air (FiO2 0.5), We investigated the incidences of PONV in each group by the Rhodes index of nausea, vomiting and retching (RINVR) at postoperative 6 and 24 hours. Results : The incidence of PONV was not different between group at each time points after surgery (at postoperative 6 hours: 20% in group R, 17.5% in group 0, at postoperative 24 hours; 12.5% in group R, 7.5% in group 0). There was no difference in the severity of PONV, satisfaction, rescue drug usage. Conclusion : Prophylactic therapy with ramosetron is as effective as conventional prophylactic therapy with ondansetron for preventing PONV in general anesthesia for laparoscopic colectomy.

Hypoallergenic and Physicochemical Properties of the A2 β-Casein Fraction of Goat Milk

Tae-Hwan Jung,Hyo-Jeong Hwang,Sung-Seob Yun,Won-Jae Lee,Jin-Wook Kim,Ji-Yun Ahn,Woo-Min Jeon,Kyoung-Sik Han2.6* 한국축산식품학회 2017 한국축산식품학회지 Vol.37 No.6

Goat milk has a protein composition similar to that of breast milk and contains abundant nutrients, but its use in functional foods is rather limited in comparison to milk from other sources. The aim of this study was to prepare a goat A2 β-casein fraction with improved digestibility and hypoallergenic properties. We investigated the optimal conditions for the separation of A2 β-casein fraction from goat milk by pH adjustment to pH 4.4 and treating the casein suspension with calcium chloride (0.05 M for 1 h at 25°C). Selective reduction of β- lactoglobulin and αs-casein was confirmed using sodium dodecyl sulphate-polyacrylamide gel electrophoresis and reverse-phase high-performance liquid chromatography. The hypoallergenic property of A2 β-casein fraction was examined by measuring the release of histamine and tumor necrosis factor alpha from HMC-1 human mast cells exposed to different proteins, including A2 β-casein fraction. There was no significant difference in levels of both indicators between A2 β-casein treatment and the control (no protein treatment). The A2 β-casein fraction is abundant in essential amino acids, especially, branched-chain amino acids (leucine, valine, and isoleucine). The physicochemical properties of A2 β-casein fraction, including protein solubility and viscosity, are similar to those of bovine whole casein which is widely used as a protein source in various foods. Therefore, the goat A2 β-casein fraction may be useful as a food material with good digestibility and hypoallergenic properties for infants, the elderly, and people with metabolic disorders.

W(110) 표면에 CO의 흡착

이경희,유위량,한현석,부진효,이순보,곽현태 성균관대학교 기초과학연구소 1998 論文集 Vol.49 No.-

The interaction of CO with W(110) surface was investigated through LEED, TDS, and photoelectron spectroscopy using synchrotron radiation under UHV condition. After CO saturation at RT, two desorption states, called α and β , were observed at about 400 and 1150 K in thermal desorption spectra, respectively. The kinetics of 3-CO followed the first order kinetics, indicating the existence of molecular CO on W(110) surface. This is contrary to the previous results. The O 1s BE(binding energy) of CO adsorbed on W(110) surface at room temperature was 529.9 eV. On the other hand, the O 1s BE of β-CO after heating to 900 K was different from that of oxygen adsorbed W(110) surface, suggesting a different adsorption state. According to the UP valence band spectra, we observed two peaks at near -10.7 eV (4σ) and -7.0 eV (5σ+1π), indicating the molecular adsorption of CO at room temperature. Furthermore we could see the 4σ peak at the various photon energy and elevated temperatures. Comparing the energy separation, Δ(4σ-1π) , between 4σ and 1π UP peaks of chemisorbed CO, we found that an increased separation reflects an decreased C-O bond strength. Therefore on the basis of TDS and photoelectron spectroscopy, we could suggest that β state of CO on W(110) may not be dissociated and has an adsorption geometry of lying-down mode.

위암과 식도암이 병발된 다발성 원발성 악성종양 1예

박사영,이나영,이효진,이선영,최진혁,이순남,심강섭,성순희,한운섭 梨花女子大學校 醫科大學 醫科學硏究所 1996 EMJ (Ewha medical journal) Vol.19 No.3

Multiple primary cancer means that more that two cancers occur independently in an individual. Recently, the incidence of multiple primary cancer has increased with lengthened survival, of cancer patients, development of new diagnostic technique and increased clinical evaluation. We report a patient who had adenocarcinoma of stomach combined with squamous cell carcinoma of esophagus simultaneously.