발표요지 : 벼의 5- Methyltryptophan 저항성주의 선발과 세포융합

이효연,김진호,김학진,귀곡수소 (龜谷壽昭) 한국식물생명공학회 ( 구 한국식물조직배양학회 ) 1993 한국식물생명공학회 춘계학술연구발표회 Vol.1993 No.-

길위에 선 사람들

이효연 열린전북 2002 열린전북 Vol.38 No.-

受粉·受精 시기를 이용한 형질전환 식물의 개발

李孝淵 順天大學校 農業科學硏究所 1994 農業科學硏究 Vol.- No.-

The herbicide bialaphos is a potent inhibitor of glutamine synthetase in higher plants. A bialaphos resistance (bar) gene encoding for an acetyltransferase was isolated from genomic DNA of Pseudmonas syringae pv tabaci. The bar gene was logated to the binary vector pBO121. Postils of tobacco plants were treated with the bar gene containing plasmid DNA at various times after pollination. When the treatment was applied at 30 and 40 h after pollination, a number of transgenic plants wrer obtained. Primary transforments (T_0 generation)and their progenies (T_2, T_3) were resistant to both bialaphos and kanamycin at a dosage lathal to untransformed control plants. Stable integration of bar gene into chromosomal DNA was proven by Southern blot analysis of genomic DNA isolated from T_1 progenies. These results show that the bialaphos resistant plants could be obtained by treatment to postils with the exogenous bar gene through the fertilization cycle of tobacco. For the gene taffing of Armoracia rusticana, maize contrilling element Ac and Ds were introduced into A. rusticana via Agrobacterium-mediated transformation method. We established an efficient in vitro regeneration and transformation system for gene transfer in A. rusticana. The optimum in vitro regenrration condition has been obtained from leaf, petiole and root organs on modified MS medium supplemented with NAA 0.1㎎/ℓ plus BA 1.0㎎/ℓ for direct shooting and with free growth regulators for root induction. For transformation, the leaf, petiole and root explants of A. rusticana were cocultivated with Agrobacterium tumefaciens, LBA4404 which carries a bianry vector pEND4K containing maixe contrilling element Ac or Ds, respectively. Selections were performed in the shoot induction medium supplemented with 100㎎/ℓ kanamycin, and 500㎎/ℓ carbenicillin. Transformation frequency showed about 8∼10% in case of leaf disks. PCR and Southern blot analyses showed that the Ac and the Ds elements wee integrated into the chromosome of donor plants.

Expression of Human Lactoferrin Gene in Transgenic Rice(Oryza sativa L.)

이효연,Yong-Eok Lee,Seong-Eun Oh,Satoshi Nishiguchi,Key-Zung Riu,송인자,박신영,Jin-Hyoung Lee,Il-Gi Kim,서석철,Seong-Lyul Rhim,임평옥 한국식물생명공학회 2007 JOURNAL OF PLANT BIOTECHNOLOGY Vol.34 No.2

Lactoferrin is an 80-kDa iron-binding glycoprotein known to exert many biological activities, such as facilitating iron absorption and having antimicrobial and anti-inflammatory effects. Rice can be a useful target for edible food plants to introduce human lactoferrin, because it has lower allergenicity and is likely to be safer than microorganisms or transgenic animals. A cDNA fragment encoding human lactoferrin (HLF) driven by the maize polyubiquitin promoter, along with herbicide resistance gene (bar) driven by CaMV 35S promoter, was introduced into rice (Oryza sativa L. cv. Dong Jin) using the Agrobacterium -mediated transformation system. Putative transformants were initially selected on the medium containing bialaphos. The stable integration of the bar and HLF genes into transgenic rice plants was further confirmed through polymerase chain reaction (PCR) and Southern blot analyses. The expression of the full length HLF protein from various tissues such as grains and young leaves of transgenic rice was verified by Western blot analysis. Analysis of progeny also demonstrated that introduced genes were stably inherited to the next generation at the Mendelian fashion.

돌연변이 벼 종자로부터 선발된 5-methyltryptophan 저항성 계통의 특성

이효연,배창휴,임용표,박노동,조백호,이수인,최해춘,김호일 한국식물생명공학회 2000 식물생명공학회지 Vol.27 No.6

Three rice (Oryza sativa L. var Dong-Jin) mutants (DTR1, DTR2, DTR3) resistant to S-methyltryptophan (5MT) were selected by mutagenized M3 seeds. The frequency of chlorophyll mutations induced by the EMS (0.2%) treatment performed 2 hours after flowering is clearly higher than that induced by other treatments in M1 generation. Progeny obtained from the self-pollinating of 5MT-resistant lines segregated with 3 : 1 of resistant to sensitive ratio. Furthermore, the ratio of homozygote to heterozygote in 5MT-resistant plants of the M4 generation was 1:2. These results show that 5MT resistance was inherited as a single dominant nuclear gene. The resistance was also expressed in callus derived from seeds. Total free amino acid content in homozygous seeds of DTR1 and DTR2 showed about 1.7 fold-increased compared to the wild-type seeds. In particular, the levels of phenylalanine and Iysine were, respectively, 6.2 and 3.2 times higher than those in the wild-type seeds. However, seeds of DTR3 had lower levels of free amino acid than the wild-type seeds. This result indicate that these mutants as a significant step towards the production of new rice with balanced amino acid content. 5-methyltryptophan (5MT) 저항성 벼의 3계통 (DTR1, DTR2, DTR3)을 돌연변이 처리된 M3세대의 종자로부터 선발하였다. Ml세대에서 엽록소 돌연변이의 빈도는 개화 2시간 후의 벼이삭에 EMS (0.2%) 처리된 실험구로부터 가장 많이 관찰되었다. 5MT저항성으로 선발된 3계통은 자식후대에 있어서도 저항성과 감수성의 비율이 3:1을 보여주었다. 또한 M4세대의 저항성 식물 중에서 자식후대의 5MT에 대한 저항성은 homozygote와 heterozygote 형태로 분리된것이 1 : 2의 비율로 관찰되었다. 이러한 저항성 돌연변이 식물은 5MT저항성 형질이 단일 우성 핵 유전자에 의해 지배된다는 것을 보여준 것이다. 또한 5MT저항성 형질은 세포수준에서도 관찰되었다. DTR1, DTR2의 homozygous 종자로부터 추출된 전 유리아미노산 함량은 야생형 식물에 비해 약 1.7배정도 높았으며, 특히 phenylalanine, Lysine의 함량이 각각 6.2, 3.2배로 증가하였다. 그러나 DTR3의 경우 야생형과 비교하여 유리 아미노산 함량의 증가는 보이지 않고 약간 감소하였다. 이러한 결과는 곡류작물의 아미노산 함량을 변화시키는 데 있어서 5MT저항성 식물체의 선발이 매우 효과적인 방법이라는 것을 보여주었다.

White Clover (Trifolium repens L.)의 수분,수정시기 방사선(60Co) 처리에 의한 잎 돌연변이 식물체의 선발

이효연,강시용,강지연,배태웅,송인자,임평옥,김해든 한국육종학회 2007 한국육종학회 학술발표회 발표요지 Vol.2007 No.6

Coated conductor에서 magneto-optical image와 scanning hall probe를 이용한 flux profile의 측정 및 분석

이효연,곽기성,이준규,유재은,염도준,Lee, H.Y.,Kwak, K.S.,Rhyee, J.K.,Yoo, J.,Youm, D. 한국초전도학회 2010 Progress in superconductivity Vol.11 No.2

The magnetic flux profiles in SmBCO and YBCO coated conductors(CC) in the presence of the external field were comparatively investigated by magneto-optic image and scanning hall probe measurements. The current distributions calculated by using the inversion method from measured field profiles show that the decrease of current densities near the edges of SmBCO CC is more significant than those of YBCO CC. Through the comparison of the numerical analysis based on Kim's critical state model and the Brandt and Indenbom's solution, we found that this feature is related to their different field dependant properties of the critical current densities.

Ethylene Imine 처리에 의해서 얻어진 벼의 5-methyltryptophan 저항성주의 특성

이효연,김진호,김학진,권병선,구곡수소 한국작물학회 1992 한국작물학회 학술발표대회 논문집 Vol.1992 No.-

EMS처리에 의한 한란의 엽록소 결핍 돌연변이 식물체의 유도

이효연,정재성,이종석 한국식물생명공학회 1998 식물생명공학회지 Vol.25 No.3

Chlorophyll mutants were produced by treating the rhizome of Cymbidium kanran with mutagen, EMS(ethyl methan sulfonate). The germination ratio of Cymbidium kanran seeds was 5.5 times higher when the seeds were treated with ultrasonic treatment for 20 minutes than untreated control. Fifty to sixty percent of the rhizomes became dark brown when they were cultured in a liquid growth medium containing 0.2% EMS for three weeks. When the dark-brown rhizomes were cultured in a solified MS medium, new rhizomes were developed from a part of the old ones. Chlorophyll mutant rhizomes were obtained from a meristem tissue by a subculturing the cuts of these new rhizomes for a year. Of the chlorophyll mutants, a zigzag-striped type of rhizome was dominant and light-yellow and albino ones were also produced. While the zigzag-striped type rhizomes were differentiated into green and striped plant, the light yellow and the white rhizomes produced yellow-striped and albino plants repectively.These results indicate that the EMS treatment on the rhizome is an effective means to induce a chlorophyll mutant. We believe that this method may be useful to produce variegated plants chlorophyll mutants from other orchids. 한란(Cymbidium kanran)의 근경에 돌연변이 물질인 EMS를 처리하여 엽록소변이 식물체를 유도하였다. 한란종자를 발아시켜서 근경을 유도할 때 종자에 40분간 초음파 처리한 것이 무처리구에 비하여 발아율이 5.5배 정도 높았다. EMS 0.2%를 포함한 근경증식용 액체배지에서 3주간 배양하였을 경우에 50-60%정도의 근경이 갈변되었다. 갈변된 근경을 근경증식용 고체배지에서 배양하였을 경우에 근경조직의 일부분으로부터 새로운 근경의 증식의 관찰되었고, 이러한 근경조직을 절단하여 1년간 계단배양하던중에 분열조직의 일부분에서 색소변이 근경이 관찰되었다. 색소변이 근경은 나선형의 줄무늬가 가장 많았고, 그 외 엷은 노란색과 흰색의 근경이 분리되었다. 나선형의 근경에서 유도된 식물체는 잎에 무늬가 전혀 없는 녹색의 식물체 또는 줄무늬가 들어 있는 식물체가 유도되었다. 그러나 엷은 노란색 근경에서 유도된 식물체는 노란색 줄무늬가 있는 식물체로, 흰색 근경으로부터 유도된 식물체는 백화된 식물체로만 분화하였다. 이상의 결과 한란근경을 이용한 색소변이식물체의 유도에 있어서 배양중인 근경에 EMS처리가 효과적인 것을 보여주었다. 이러한 방법을 다른 식물에 적용하면 색소변이 식물체를 개발할 수 있으리라 생각된다.

[행복 일기] 요리로 지지고 볶는 수학공부

이효연 샘터사 2009 월간 샘터 Vol.473 No.-