SDS-PAGE에 의한 이락촌폐흡충 항원단백질의 분석

박우삼,이옥란,남해선 순천향대학교 1993 논문집 Vol.16 No.4

Crude and affinity purified antigens(PISA, PINA and PIBA) of Paragonimus iloktsuenensis were analyzed by SDS-PAGE(SDS-Polyacrylamide Gel Electrophoresis). According to EITB(Enzyme linked Immunoelectro Transfer Blot), antigenic protein fractions seperated on SDS-PAGE gel was transferred to nitrocellulose paper(NC paper) and transferred fractions on NC paper reacted to rat sera infected with P. iloktsuenensis. In SDS-PAGE, major protein fractions in crude and purified antigens of P. iloktsuenensis were found at molecular weight(MW) 10-12kd, 27-28kd, 32-34kd and 36kd. In comparison of saline extract crude antigens(PISA and PWSA) of P. iloktsuenensis and P. westermani, many protein fractions were observed in same molecular weight. In EITB, major antibody binding protein fractions of crude antigen (PISA)were MW 27.5-28.5kd, 33kd and 36-37kd and of purified antigen (PIBA) were MW 27.5-28.5kd and 33-35kd.

측정방법에 따른 치과용 수경성 시멘트의 경화시간

박정우,기형춘,변상기,이해형 大韓齒科器材學會 2001 대한치과재료학회지 Vol.28 No.4

Information on setting behavior of luting cements is essential to accomplish successful cementation of dental restoration. The purpose of this study is to investigate the influence of test variables on the setting time of dental water-based luting cements. Six kinds of luting cements were investigated: two zinc phosphate, two zinc polycarboxylate and two glass ionomer cements. Cements were mixed with the standard consistency for luting according to their respective manufacturer's recommendation. Setting time of cements was measured with two test apparatus (penetrating indentor and oscillating rheometer) under one of following environmental conditions (temperature/humidity); 37℃/90%, 23℃/50% and 37℃/20%. The Setting time was recorded as the time elapsed from start of mixing to the time indicated at the ISO specification (9917:1991) or when the oscillation is reduced to 95% of the original width. Setting time of luting cements was decreased with increase in temperature and decrease in humidity, and it was significantly influenced by environmental temperature and humidity. Some cements tested showed significant differences in setting time measured between indentor and rheometer under laboratory environment. Percent water loss of cements was different under various environmental conditions. This was largely arisen at the early stage of setting. Zinc Phosphate was hygroscopic under high humidity. these results indicate that working and setting time should be separately evaluated in office and oral environments, respectively. Oscillating rheometer was useful to investigate setting behavior of dental water-based luting cement. However, control of test environment and sensitivity should be considered in the rheometry.

Acetazolamide가 적혈구막의 Na-K-ATPase 활성도에 미치는 영향

이우영,박해근 충남대학교 의과대학 지역사회의학연구소 1986 충남의대잡지 Vol.13 No.1

This study was attempted to investigate the action of acetazolamide on the Na-K- ATPase activity in the rabbit RBC membrane. The red cell ghost was obtained by means of Rossenberg and Guidotti and the amounts of isolated inorganic phosphorus as an index of Na-K-ATPase activity was determined by the method of Fiske and SubbaRow. The Na-K-ATPase activity was expressed as the unit of μM Pi/10min/g-protein. The results obtained are summarized as follows. 1. The Na-K-ATPase activity of the RBC membrane was related to incubation time. but the activity per g-protein was large at the 10 min incubation. 2. The Na-K-ATPase activity was related with protein concentration of incubation medium, while there were no difference the activity per g-protein relatively greater in 90 mg/ml protein. 3. 0.5 and 10.0 mg/ml of acetazolamide was inhibit the Na-K-ATPase activity while 5.0 mg/ml of it enhanced the activity. From the above results, it was suggested that the acetazolamide affect Na^+ transport across the cell membrane by alter the Na-K-ATPase activity.

CT flouroscopy를 이용한 중재적 시술의 유용성

전혜정,박정희,서보경,박상우,신현준,최영철,한혜승,이창희 건국대학교 의과학연구소 2004 건국의과학학술지 Vol.14 No.-

Conventional CT-guided needle localization has been reported many times, but a few reports of interventional procedures by using multidetector CT scan with CT-fluoroscopy have been described. We presented 36 cases of CT-fluoroscopy guided interventional procedures focusing on the puncture technique. Two cases of small pneumothorax and one case of pulmonary hemorrhage out of 2lcases after lung biopsy, were not needed any treatments We can easily and accurately access to the lesion by using CT-fluoroscopy and unique our marker system.

Prostaglandin이 家兎 腎皮質의 Na-K-ATPase 活性度에 미치는 影響

李星雨,朴海根 충남대학교 의과대학 지역사회의학연구소 1985 충남의대잡지 Vol.12 No.1

Renal prostaglandins(PGs) are synthesized in both the cortex and the medulla. PGs modify the renal excretion of salts and water. This effects of PGs are related to several different sites of action in the kidney that include : 1) direct inhibitory effects on ion transport in the distal tubule: 2) enhancement of basal water permeability of the collecting tubule and inhibition of the hydroosmotic effect of vasopressin. On the other hand, Na--K-ATPase act as carrier of active Na^t transport across the cell membrane. This study was attempted to investigate the action of PGs on the Na-K-ATPase activity in the renal cortical homogenate. PG E_2 and F_2αconcentration in the incubation medium were 100, 10, 1.0 mg/ml, respectively. The results obtained are summarized as follows: 1. Total ATPase and Na-K-ATPase activity of the renal cortical homogenate were 15.11±0.64 and 1.65±0.18 μM/10min/mg protein. 2. Total ATPase activity in PG E_2 concentration of 100㎍/ml was significantly inhibited as 80.4±5.6% to the control value. 3. PG E_2 inhibited the Na-K-ATPase activity. Especially, 1.0㎍/ml in concentration significantly inhibited the Na-K-ATPase activity as 75.4±19.8 % to the control value. 4. PG F_2α also inhibited the Na-K-ATPase activity. Especially. 1.0 ㎍/ml in concentration significantly different. From the above results, it was suggested the action of PG E_2 and PG F_2α on the kidney was mediated by inhibition of renal cortical Na-K-ATPase activity.

부부상호간의 커뮤니케이션이 주부의 가정관리능력에 미치는 영향

이정우,박혜경 숙명여자대학교 생활과학연구소 1992 生活科學硏究誌 Vol.7 No.-

The purpose of this study is : (1) to identify the overal level of communication between husband and wife, home management ability (2) to investigate the relations between communication and home management ability (3) to examine the factors affecting the home management ability. For the purpose of this study, I selected the samples from the wives living in seoul, who have husband and children. Among a total of 600 respondents, 418 wives were finally selected as a data sources, and the other were discarded. The data were analyzed by the statistical methods such as the frequency, percentile, t-test, F-test, person's correlation, adn stepwise multiple regression analysis. The significant results are as follows: (1) The communication between husband and wife score was 3.22. To refer to subclassfication, the communication time between husband and wife was two hours for a day. The level of communication style was above average and this imply opened style. The communication frequency was under average, communication agreement was above average. And, this communication between husband and wife differs significantly according to the level of education (husband, wife), income. (2) The management ability score was 2.40. In behavior specific, the home management ability score of control coordination and guidence were relatively high, and that of evaluation and planning were relatively low. In object specific, the home management ability score of money was the highest, and that of knowlege and skill were the lowest. This home management ability differes significantly, according to the level of wife's education, number of children, income, husband's occupation. (3) There were positive correlation between the communication and home management ability. (4) The home management ability was independently affected by the following variables in order : communication agreement, the level of wife's education. Based on the above results, I think that the extension of the communication between husband and wife need, and this will promote wife's home management ability. In addition to that, it is nessary to develop a standard instrument to measure the home management ability effectively.

용사코팅용 나노 WC-12Co 피드스톡의 제조

전형우,김주선,박준영,박종구,황순영,이해원 대한금속재료학회 2004 대한금속·재료학회지 Vol.42 No.4

This study investigates the fabrication of WC-12Co feedstock powders with weakly locculated slurries by spray drying under various conditions. The morphological defects and flocculation states according to the amount of additives are evaluated to prepare spherical WC-Co feedstock powders with little morphological defects and homogeneous microstructures. The characteristics of feedstock powders, heat treated between 850-1200℃, are analyzed via SEM, EPMA, and mercury porosimetry. The results showed that with the increase in heat treatment temperature the portion of larger pores around 10 μm increased while, that of smaller pores of 1 μm lessened due to the coalescence of WC particles, forming dense feedstock powders with little defects such as hollow or craters. The feedstock heat treated at 1200℃ showed a porosity of 42.2% and a density of 8.28 g/cm^(3) as well as uniform distribution of pores and Co. (Received January 19, 2004)

대구 성서지역 악취 민원발생일의 기상학적 특성

김해동,구현숙,정우식,최성우,안지숙,박명희 한국환경과학회 2003 한국환경과학회지 Vol.12 No.10

We studied the relationship between a civil affair occurrence of bad smell and meteorological conditions around Seongseo district for recent 2 years. The results were as follows; (1) The civil affair occurrence days of bad smell were concentrated from May to August. (2) Daily mean temperatures were mostly 20~28℃ and daily mean relative humidities were 60~80%. (3) Wind speeds were mostly less than 2m/s(75%). and wind directions were southerly winds(50%). It was hardly occurred for relatively well-ventilated conditions in cases of wind speeds 2m/s over. And the Lagrangian particle dispersion model were used to figure out the transport route in a civil affairoccurrence days of bad smell. It was found that the south-western winds transported the bad smelling materials from Jungni-dong(the place of source) to the housing complex located along a piedmont district.

다발성 대장암 간전이 환자의 간절제를 위한 새로운 시도

주종우,김형철,임철완,신응진,조규석,유기원,송옥평,홍대식,박성진,조준희,이혜경,김희경,권계원,고은석 순천향의학연구소;Soonchunhyang Medical Research Institute 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

Multiple bilobar liver matastases (MBLM) are the main cause of low resectability in the colon cancer liver metastases. The authors experienced one case of initially non-resectable colon cancer liver metastases. He was curatively and safely treated with a two-stage hepatectomy using the new method of future remnant liver volume growing. A 54-year-old man was referred to our department with the sigmoid colon cancer combined with MBLM, which were checked in two small metastatic lesions in the left lobe and five large sized lesions in the right lobe in the computed tomogram (CT). A laparoscopic assisted anterior resection was primarily performed. We performed the 1^(st) stage hepatectomy 3 weeks after the colon resection. Intra-operative Ultrasonogram (US) found 9 small superficial metastatic lesions in the left lobe. All that lesions were completely removed by non-anatomical wedge resection. An occlusion ballon catheter was placed in the right portal vein through a small branch of the inferior mesenteric vein at that time. The future remnant liver volume was sufficiently increased 3 weeks after the 1^(st) hepatectomy. A right hepatectomy was safely performed 22 days after the 1^(st) hepatectomy. The patient received a regional chemotherapy (interleukin2 based immuno-chemotherapy through hepatic artery) for 4 months, then received 9 cycles of systemic chemotherapy (biweekly Oxaliplation, leucovorin, plus 5-fluorouracil) without any recurrence evidence.

응급실에 내원한 약물중독환자의 지역별 비교분석

임근우,박일영,유승진,나병호,오동렬,전해명,황주일,김세경 대한응급의학회 1995 대한응급의학회지 Vol.6 No.1

We reviewed retrospectively 230 patients who came to emergency room of Kang Nam St. Mary's Hospital and Uijongbu St. Mary's Hospital between 1. January and 31. December 1994. We classified them according to their age, sex, a sort of drug, the cause of poisoning, prehospital care, time-lapse to arrive at hospital after poisoning, psychiatric problem the status of patients. *We conclude as follows; 1. The male to female ratio was totally 1 : 1.98, 1 : 1.24 in a urban area, 1 : 2.43 in a rural area. 2. On age of the patients in this study, the peake incidence was between 20 and 39(55.2%). 3. On seasonal prevalence, the poisoning was most common in summer and spring, in urban area(35.7%) and rural area(37.0%) respectively. 4. The common drug were as follow; Doxylamine, Organophosphate, Acetaminophen in urban area, Paraquat, Organophospate, Doxylamine in rural area 5. The percentage of suicidal attempt were 81.8% and 87.7% in urban area and rural area respectively. 6. The poisoning patients had psychatric problem in 19.4% and 24.6%, in urban area and rural area respectively. 7. Before arrival to emergency room, 27.7% of patient was done prehospital care in rural area. 8. On arrival to emergency room, 95.1% and 73.8% of patients show stable vital sign in urban area and rural area respectively.