Effect of Ionizing Radiation on Rat Tissue: Proteomic and Biochemical Analysis

Park*, Eui‐,Chul,Yoon, Jong‐,Bok,Seong*, Jin‐,Sil,Choi, Kyoung‐,Soo,Kong, Eung‐,Sik,Kim, Yun‐,Jeong,Park, Young‐,Mee,Park, Eun‐,Mi Taylor Francis 2006 Preparative biochemistry & biotechnology Vol.36 No.1

<P>Reactive oxygen species (ROS), generated by ionizing radiation, has been implicated in its effect on living tissues. We confirmed the changes in the oxidative stress markers upon irradiation. We characterized the changes in the proteome profile in rat liver after administering irradiation, and the affected proteins were identified by MALDI-TOF-MS and ESI-MS/MS. The identified proteins represent diverse sets of proteins participating in the cellular metabolism. Our results demonstrated that proteomics analysis is a useful method for characterization of a global proteome change caused by ionizing radiation to unravel the molecular mechanisms involved in the cellular responses to ionizing radiation.</P>

Brief introduction of Butterfly(Lepidoptera) from Myanmar

Ja Lang LIM,Jinyoung PARK,Ik Je CHOI,Yong Kyun OH,Eui Seok JEONG,Dong Pyeo LYU,U Pho Cho,Khin Mar Myint,Khin Mar Yi,Jong Kyun PARK 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.04

Myanmar is located in the south-east Asia peninsular and it is flanked by five neighboring states: Bangladesh, India, China, Laos and Thailand. It is located 10~22 degree north latitude, to 92~101 degrees east longitude. Myanmar has a tropical climate with three seasons in Myanmar. Hot season (March through April), rainy season (May through October) and the cool season (November through February). The coastal regions can get very hot and humid, especially before the rainy season. Myanmar has a monsoon season from May to October when there are heavy rains and often flooding. The investigation was carried out during the dry season(2013) in Nat Ma Taung National Park and Popa Mountain Park. Popa Mountain Park is located in Kyawpadaung Township, Myinchan district, Mandaly Division. The area is 49.63 square miles. Its coordinates are 29°31'60" N and 27°43'60" E. Nat Ma Taung National Park, Myanmar treasure is in Mindut District of Chin State and its area of 279 square miles. It is the third highest mountain in Myanmar with the height of 10,500 feet. Its coordinates are 21°14'00" N and 93°54'00" E. As the result, butterflies(Lepidoptera) collected in the two regions were about 60 species of 7 familys. Collecting dates, expedition activities, photo of main specimen and information of Myanmar were provided.

저항성 스트립의 전자파 산란 패턴 제어에 관한 연구

박의준,최종인 金烏工科大學校 1998 論文集 Vol.19 No.-

A mixed potential integral equation(MPIE) for the induced current on a cylindrical perfect conductor with arbitrary thin cross-section is considered. The equation is applied to a strip for control of scattering field. The control is carried out by prediction of the current distribution for a prescribed pattern and than by extraction of resistance distribution on the strip using the relationship of each distribution which is established by physical optics. Examples of an efficient control is given with the appropriate optimization process for the nonuniform distribution functions.

분산 데이터 베이스 시스템에서의 충돌 그래프분석 기법에 관한 연구

박종원,최의인 충남대학교 자연과학연구소 1989 忠南科學硏究誌 Vol.16 No.1

The CGA(Conflict Graph Analysis) technique is used for preanalyzing of transaction when we design a data base. The CGA technique is mainly concerned with the design and implementation of a tool for this preanalysis operation. This technique classify's specification of transaction on classes, checks the conflict among them, and selects appropriate protocols for each pair of transaction classes.

사무직 근로자에서 직무스트레스 및 VDT작업과 목·어깨 근골격계 증상과의 관련성

이의철,김환철,정달영,김동현,임종한,박신구 대한산업의학회 2007 대한직업환경의학회지 Vol.19 No.3

현행 태권도 경기규칙 제도 개정방안에 관한 고찰

진중의,김종길,박준석 龍仁大學校 1997 용인대학교 논문집 Vol.14 No.-

It will be the crucial condition to provide people with the excellent and interesting game so that the techniques of competition would be advanced and everyone loves Taekwondo as National sport, which will be adopted in Sidney Olympic gasses. And it will help Taekwondo be adopted permanently. We suggested a scheme of partly developed competition rules on the current Taekwondo. Each of us thinks only theoretically that this direction of contents will be the standards for advancement of Taekwondo competition. But we wrote this paper with the belief that the contents of Taekwondo competition developed should be scientific and reasonable in order that they can be the new start point for Taekwondo advancement, The followings are my suggestion for Taekwondo advancement 1) It is necessary to change the rules, so it should be possible to select the various colors of ground and uniforms, five-minute gave and gradation credits. It will make Taekwondo be loved by the whole world and be adopted permanently in Olympics. 2) It is necessary to adjust the grades of each weights corresponding to international standard height and to found the more professional Taekwondo teams so that they can activate the amatuer Taekwondo players. 3) The main basic principles of Taekwondo are based on the philosophical thoughts. It is necessary to study the detailed programs according to pratice-process from the martial and competitional aspects corresponding to grades and ages. 4) It is also necessay to hold competition on Pumsae. 5) It should be realized to build the create Taekwondo Meca Center, which represents the holy Korean spirits.

유전특성에 미치는 선형 저밀도 폴리에틸렌과 에틸렌 비닐 아세테이트 혼합비 영향

김원종,정의남,임윤희,심낙순,박하용 三陟大學校 2002 論文集 Vol.35 No.-

The physical and electrical properties of electrical insulating materials due to the mixing ratio of linear low density polyethylene(LLDPE) and ethylene vinyl acetate(EVA) are studied. Specimens are selected as LLDPE and EVA of mixing ratio of 50 : 50, 60 40, 70 : 30, 80 20 and virgin. To analyze the physical properties of specimens, Fourier transform infra-red spectrum, X-ray diffraction and differential scanning calorimetry are used. To investigate the influence on dielectric characteristic due to Physical structural change of specimens, we investigated the frequency and temperature dependency of tan f for virgin specimen and mixture specimens in the temperature range of 25[t]~120['c], frequency range of 30[Hz] ~l.5×10s[Hz]. For the value of tan 3, dielectric loss, at each temperature of 35, 75, 100, 120[℃], it is confirmed that as applied voltage increase, α and β peak moved to low frequency area at high temperature area, and also moved to high frequency at low temperature areas, the width of peak widen. it is considered to be caused by the molecular vibration, reduction of side-branch density, reduction of crystalline with increasing temperature.

중합효소연쇄 반응법에 의한 인형거대세포바이러스(human cytomegalovirus)의 신속한 검출

김의종,오명돈,박기호,신형식,이환종,김남중,최강원 대한감염학회 1996 감염 Vol.28 No.4

목 적 : 인형거대세포바이러스(HCMV)는 면역 저하 환자에서 폐렴, 망막염, 간염 등 치명적인 감염증의 원인이다. 전통적인 세포 배양법으로 HCMV를 분리하기까지는 1주에서 4주가 걸리므로 이 바이러스에 의한 질환이 의심되는 경우 신속한 진단법을 이용한 진단이 바람직하다. 저자들은 한국에서 분리되는 야생주 HCMV를 검출하는데 중합효소 연쇄 반응법을 이용할 경우의 진단적 유용성을 알기 위하여, 전통적인 세포배양법으로 HCMV가 분리된 검체를 이용하여 중합효소 연쇄 반응법의 특이도와 민감도를 평가하였다. 방 법 : 국내 야생주 HCMV 15주, 그리고 전통적인 바이러스 배양법으로 HCMV가 분리된 소변 15검체를 대상으로 중합효소 연쇄 반응을 실시하였다. 중합효소 연쇄 반응은 Towne주의 immediate early antigen의 유전자에서 유리된 primer MIE와 AD169주의 immediate early antigen의 유전자에서 유리된 primer IE를 이용하였다. 증폭 산물은 겔 전기영동 후 EtBR으로 염색하여 자외선 하에서 관찰하였다. Primer IE에 의한 증폭산물은 또한, DNA blot hybridization 방법으로 확인하였다. 결 과 : 1) 국내 HCMV 야생주 15주를 대상으로 중합효소 연쇄 반응을 한 결과 primer IE를 이용한 경우에는 100%(15/15)에서 177bp의 증폭 산물이 관찰되었고, 이들 증폭 산물은 모두 probe IE와 보합 결합되었다. primer MIE를 이용한 경우에는 93%(14/15)에서 435bp의 증폭 산물이 관찰되었다. 2) 세포 배양법으로 HCMV가 분리된 소변 15검체를 대상으로 primer IE를 이용하여 중합효소 연쇄 반응을 한 결과 direct gel analysis 법으로는 73%(11/15)에서, 보합 결합법으로는 87%(13/15)에서 관찰되었다. Primer MIE를 이용한 경우에는 direct gel analysis법으로 87%(13/15)에서 435bp의 증폭 산물이 검출되었다. 결 론 : Primer IE와 MIE를 이용한 중합효소 연쇄 반응법은 국내 야생주 HCMV를 신속히 검출하는데 유용한 검사법이다. Background : Human cytomegalovirus(HCMV) can cause pneumonitis, hepatitis, retinitis and other serious diseases in the immunocompromised patients. It takes 1 to 4 weeks to diagnose HCMV infection by conventional virus culture. Therefore, when HCMV diseases are suspected, a rapid diagnostic method such as polymerase chain reaction(PCR), antigen assay or shell vial culture is desirable. We evaluate the sensitivity and specificity of a PCR for the rapid detection of HCMV wild strains in Korea. Methods : We used 2 sets of primers ; primer IE and primer MIE derived from the sequence for immediate early gene of AD169 strain and Towne strain, respectively. Fifteen clinical isolates of HCMV, suspended in MRC-5 cells, were amplified by PCR. Fifteen urine specimens which were positive for HCMV by conventional virus culture were also amplified. Amplification products were analyzed by polyacrylamide gel electrophoresis. The products from PCR with primer IE were also identified by DNA blot hybridization. Results : PCR using primer IE gave the PCR products in all of the 15 HCMV wild strains. All of these were hybridized with probe IE. When primer MIE were used, 93%(14/15) of the wild strains showed amplified bands by direct gel analysis. When the urine specimens were amplified by PCR with primer IE, amplified bands were seen in 73%(11/15) by direct gel analysis ; 87%(13/15) by hybridization method. When primer MIE were used, 87%(13/15) of the urine specimens showed the PCR products by direct gel analysis. Conclusion : Polymerase chain reaction with primer IE and MIE may be a specific and sensitive diagnostic method for rapid detection of HCMV wild strains in Korea.

영·유아 언어, 인지, 사회·정서 발달 평가도구 문항 개발을 위한 예비연구

이종숙,신은수,박은혜,김영태,유영의,최일선,유흥옥 이화여자대학교 교육과학연구소 2009 교육과학연구 Vol.40 No.3

본 연구는 만 2~5세 영·유아의 발달 상태를 객관적으로 평가함과 동시에 발달이 지연된 영·유아를 선별할 수 있는 발달평가도구 문항을 개발하기 위한 예비연구이다. 이를 위해 문헌고찰 및 발달관련 평가도구들을 비교 분석하여 발달지표를 선정하고, 만 2세~5세까지의 영·유아의 언어, 인지, 사회·정서발달영역을 교사관찰에 기초하여 평가할 수 있는 문항을 개발하였다. 연구대상은 대도시, 중소도시, 읍면 소재지 별로 표집 된 만 2세부터 5세까지 총 13개 유아교육기관에 다니는 435명의 영·유아들이며, 본 연구에서 최종 개발된 평가도구의 문항은 총 141문항이다. 평가도구 문항은 언어, 인지, 사회·정서발달영역별로 영·유아의 행동과 언어를 중심으로 교사가 관찰하고, 관찰하기 어려운 문항에 대해서는 단서와 kit를 제시하여 평가할 수 있도록 구성하였다. 개발된 평가도구 문항의 적절성을 알아보기 위하여 내용 타당도와 내적 신뢰도를 살펴본 결과 비교적 양호한 것으로 나타났다. This was a preliminary study to construct a developmental rating scale for understanding children's objective developmental levels and screening developmental disabilities. For this purpose, a developmental indicator was selected by a literature review and comparative analysis of related developmental assessment tests. An assessment scale based on teacher's observations to measure language, recognition, and social-emotional developmental areas was developed. The subjects were 435 young children aged 2 to 5 who lived in both urban and rural areas. A trial test consisted of 141 assessment items in the language, recognition, and social-emotional developmental domains. The developmental scale was evaluated by teachers who observed children's behavior and language, and used clues and kits. Tests on validity and reliability for confirming the appropriateness of the developed assessment scale showed that content was relatively valid and internally consistent.

유도 '되치기 본'의 개선 방안에 관한 연구

김의환,김도준,김규수,김관현,김종달,최종삼,조용철,박순진,윤익선,안병근,정 훈,김미정,한성철 龍仁大學校 武道硏究所 1999 武道硏究所誌 Vol.10 No.1

The purpose of this study was to reform practically a Korean Judo's Doechigi-Bon(Forms of Counterattack, judo's Gaeshi no Kata, Judo's Uradori no Kata, Judo's Gonosen no Kata) that was established in 1955 Korea, according to changing of techniques by Judo's modernization, in order to have Judo's carefulness and systematic diffusion. Reform procedure of Judo's Doechigi-Bon was 1st stage, Questionnaire survey 303 judokas, 2nd stage, Technical seminar by judo experts(12 judo professor) 4 times, 3rd stage, wording report for reform, 4rd stage, Discussion and judgement of Teaching and Judgement commission of Korean judo Association(KJA), 5th stage, Public hearing for reform in KJA, 6th stage, Report and decision of board of directors in KJA, 7th stage, public publication of Judo News(No.53) in KJA. Basic principle of reform of Judo's Doechigi-Bon were as table 1. Table 1. Basic principle of reform of Judo's Doechigi-Bon ------------------------------------------------------------------------------- Items Reformed Key Points of Judo's Doechigi-Bon ------------------------------------------------------------------------------- 1. Structure 1) Grand classification : Classified by 3 parts(1,2,3Gyo) 2)Medium classification : Te waza, Goshi waza, Ashi waza devided per each part(Gyo) 3)Sub-classification : Classified five techniques per each part(Gyo) 2. Contents 1) Selected established techniques as possible 2) Considered rationalty and overlapping of counterattack techniques 3. Decision of Conterattack techniques 1) Refered to results of Basic Questionnaire survey 2) Priority to decisions of Judo expert technical seminar -------------------------------------------------------------------------------- Reformed Korean Judo's Doechigi-Bon(Forms of Counterattack techniques-KJA, 1999) are as follows: 1. Gyo; ① Uki otoshi -> Uchi mata ② Harai goshi -> Harai goshi gaeshi ③ O soto gari -> O soto gake ④ Ko uchi gari -> Sasae tsurikomi ashi ⑤ O uchi gari -> Ko soto gari 2. Gyo; ① Ippon seoi nage -> Okuri eri jime ② Tsuri domi goshi -> Uki waza ③ Okuri ashi harai -> Okuri ashi harai ④ Ko soto gari -> Tai otoshi ⑤ Hiza guruma -> Hiza guruma 3. Gyo; ① Kata guruma -> Sumi gaeshi ② Tai otoshi -> Ko soto gari ③ Hane goshi -> Harai tsurikomi ashi ④ Uchi mata -> Tai otoshi ⑤ Tomoe nage -> O uchi gari