비글개에서 신원방우황청심원의 급성 및 아급성독성시험

성하정,권오경,방명주,곽형일,이진영,신대희,박대규,정규혁,윤효인,조명행 성균관대학교 약학연구소 1998 成均藥硏論文集 Vol.10 No.1

Single and 4 weeks oral administration of New wonbangwoohwangchungsimwon (NSCH) which was used l-muscone as substitutive material of musk, to beagle dogs of both sexes were performed to investigate both acute and subacute toxicity. Beagle dogs (3 males and 3 females) in acute experiments were administered orally with sinlge dose of 2,000 ㎎/㎏ and groups of 9 male and 9 female beagle dogs in subacute experiments were given daily different dosage of NSCH, 160 ㎎/㎏/day (low dosage group), 400 ㎎/㎏/day (middle dosage group), 1,000 ㎎/㎏/day (high dosage group) once a day for 4 weeks by oral route according to the Established Regulation of Korea Food and Drug Administration (1996. 4. 16). LD_50 value for beagle dogs was more than 2,000 ㎎/㎏ per oral for both male and females. In animals administered with NSCH, there were neither dead animals nor significant changes of body weights. In addition, no differences were found between control and treated groups in clinical sign, urinalysis, eye examination, hematology, serum chemistry, organ weight and other fingings. No histological lesions were observed in both control and treatment groups. Above data strongly suggset that NSCH in beagle dogs is considered to be safe.

악성 신경교종에서 기관형적 배양후 형성된 미세종양 구상체의 초미세구조적연구

신형진,정천기,박인성,정영섭,정희원,한대희 대한신경외과학회 1993 Journal of Korean neurosurgical society Vol.22 No.3

The present study investigated not only the feasibility of organotypic spheroid culture system taken from human malignant gliomas but also the similarities and differences between surgical specimens and cultured spheroids using light microscopy electron microscopy, and flow cytometric examination. Surgically resected tumor specimens from eighteen human malignant gliomas were minced and explanted into agarose-coated culture wells. After three to five days these microtumor fragments emerged as spheroids and could be maintained as organotypic spheroids for more than eight weeks. Measurements of the spheroids showed that they decreased during the initial two to three weeks and afterwards remained unaltered over a specific period of time. This growth pattern of the spheroids was consistent with the condition of tumors in vivo suggesting the linkage of cell proliferation and loss. Light microscopic and electron microscopic studies of the spheroids demonstrated that morphological structures were similar to those of the original tumor tissue in vivo and histopathologic characteristics of the original tumor were maintained over a long culture period. The spheroids contained connective tissues, blood vessels, macrophages and neutrophils maintaining a three-dimensional architectural resemblance to the original tumors. Of three pairs of the surgical and spheroid specimen examined by the flow cytometry, one showed a change of ploidy pattern and two contained increased fictions of proliferating cells. It is concluded that this microtumor spheroid system can maintain the characteristics of the original tumors and may serve as an alternative to the in vivo xenograft model for the research of brain tumor biology, invasion and immunology while providing a valuable technique for the evaluation of new therapies such as biological response modifiers.

CASE 정보저장소에 관한 연구

방상원,고형대 木浦大學校 情報産業硏究所 1995 情報産業硏究誌 Vol.3 No.-

In developing software systems, how to build the repository and how to save the information systematically is crucial for tools of software environment or CASE environment to execute their jobs successfully. To increase productivity, each tool not only should manage and operate the type of information which is unique to it, but also should share the information and data so that they do their jobs inter-relatedly. In this paper, we investigated the general concepts and architecture on CASE repository. We also design IRDS repository conceptually.

동종 골수 이식한 백혈병에서 단반복 유전자로 살펴본 혼합 키메라 현상의 의의

장대영,이정신,서철원,이규형,이제환,지현숙,박찬정,한면수,최동원,김정균,최성준,김성배,김상위,김우건,김상희 대한조혈모세포이식학회 1998 대한조혈모세포이식학회지 Vol.3 No.1

연구배경 : 동종 골수 이식한 백혈병에서 키메라 현상의 분석은 착상, 질병 재발과 이식 거부를 이해하는데 도움을 줄 수 있다. 최근 STR을 PCR로 증폭한 검사법에 의해 매우 민감하고 유용하게 혼합 키메라 현상을 검출할 수 있다고 보고되고 있으나, 이 방법의 민감도와 임상적 의의는 아직 완전히 정립되지는 않았다. 따라서 PCR-STR검사법이 혼합 키메라 현상을 민감하게 검출하는지 보고, 동종 골수 이식 후 혼합 키메라 현상의 변화 양상을 관찰하고, 키메라 현상의 임상적 의의를 보고자 하였다. 방법 : 형제로부터 동종 골수 이식한 급성 백혈병 6명과 만성 백혈병 2명의 모두 8명을 대상으로 하였다. 44개 골수 검체에서 phenol/chloroform방법에 의하여 단핵세포 DNA를 추출하였다. DNA는 CTT 삼중체, vWA와 amelogenin시발체를 이용하여 증폭하였다. 증폭 산물은 5% 폴리아크랄아미드 젤에서 전기영동 뒤 은 염색으로 확인하였다. 2명의 DNA를 여러 비율로 혼합하여 상기 검사를 하여 검출 가능 최소 농도를 결정하였다. 결과 : 상기 검사법의 검출 가능 최소농도는 0.25%였다. 혼합 키메라 현상은 8명중 4명에서 관찰되었다. 혼합 키메라 현상은 보인 1명에서 급성 및 만성 이식편대 숙주 질환이 나타났고, 완전 공여자 키메라 현상을 보인 1명에서 만성 이식편 대 숙주 질환이 나타났다. 점진적으로 증가하는 혼합 키메라 현상을 보인 2명은 이식 후 12개월에 질병 재발을 보였으나, 완전 공여자 키메라 현상을 보인 경우엔 재발이 관찰되지 않았다. 결론 : PCR-STR검사법은 동종 골수 이식 후 남아 있는 환자 세포를 검출하고 키메라 현상을 관찰하는데 유용한 방법이다. 또한 이 검사법은 동종 골수 이식 후 재발의 조기 진단, 이식편 대 숙주 질환의 평가와 면역 관용에 임상적으로 응용할 수 있으리라 생각한다. Background : Chimerism analysis after allogeneic bone marrow transplantation (BMT) for leukemia could be helpful understanding of the early marrow engraftment, disease relapse, and graft rejection. Recently, a PCR technique which amplifies short tandem repeats(STR) has been reported to be highly sensitive and reliable in detecting mixed chimerism. But its sensitivity and clinical significance has not been established. The purpose of this study was firstly, to confirm whether a PCR-STR in highly sensitive enough ti assess mixed chimerism, secondly to monitor the changing patterns of mixed chimerism after allogeneic BMT, and lastly to determine correlation between the chimeric status and the clinical outcome. Methods : A study was made eight patients (six with acute leukemia and two with chronic leukemia) who underwent unmanipulated allogeneic BMT along with matched donors. DNA was extracted from mononuclear cells of 44 bone marrow samples using a phenol/chloroform method. Amplification of DNA was done using CSFIPO-TPOX-TH01(CTT) triplex with or without vWA or amelogenin primer. The amplified product was separated on 5% polyacrylamide gel and was confirmed by silver staining. The sensitivity of CTT triplex method was determined by mixing DNA from two person in serial proportion using the same method. Results : The sensitivity for CTT triplex method was 0.25% Mixed chimerism was documented in 4 of 8 patients. ONe of four patients with mixed chimerism developed acute and chronic graft versus host disease (GVHD) and one of four patients with complete donor chimerism also developed chronic GVHD. Two patient with progressive mixed chimerism relapsed at 12 months post-BMT, whereas no patient with complete donor chimerism has relapsed. Conclusion : The study has found that PCR-STR was an effective method for the detecting residual host cell and monitoring the chimeric status after allogenic BMT. PCR-STR can also be clinically applicable in early prediction of relapse, appropriate assessment of GVHD, as well as tolerance after allogeneic BMT.

천측두 동맥류 1예

강성호,임대준,김동욱,김보형 건국대학교 의과학연구소 1998 건국의과학학술지 Vol.8 No.-

Superficial temporal artery aneurysm is a rare entity, which is related to blunt trauma. It was diagnosed with history, physical examination and CT or angiography. Aneurysm is simply treated with excision and ligation. We report a case with review of literature.

재발 또는 불응성 비호즈킨 림프종 환자에서 CDME 구제항암화학요법 후 고용량 항암화학요법 및 자가말초혈액 조혈모세포이식의 효과

김세형,한강원,배상병,김찬규,이남수,이규택,박성규,원종호,홍대식,박희숙 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

Background and objectives : The long-term survival in patients with non-Hodgkin's lymphoma (NHL) after conventional dose chemotherapy is about 35% and the rest of the patients tend to have relapse. So, in relapsed or refractory NHL, we compared the outcome of patients undergoing high-dose chemotherapy with autologous peripheral blood stem cell transplantation(APBSCT) with only salvage chemotherapy of cisplatin, dexamethasone, mitoxantrone, and etoposide(CDME). Materials and methods : From June 1993 to December 1999, 25 patients with relapsed or resistant NHL were treated with CDME regimen as salvage chemotherapy. Twelve patients were received four cycles of CDME chemotherapy, and 13 patients were received high-dose chemotherapy with APBSCT following two cycles of CDME chemotherapy. Results : The median follow-up duration was 12.8 months(range:4-68). The overall response rate was 41.7% (complete response rate 25%, partial response rate 16.7%) in 12 patients with CDME only. Thirteen patients who were treated with high-dose chemotherapy with APBSCT achieved 61.5% complete response rate and 15.4% partial response rate, with an overall response rate of 76.9%. The estimated 3-year progression-free survival rate was significantly higher among patients who received high-dose therapy than patients who received CDME only(41.5% vs 20.0%, p<0.05). And, 3-year overall survival rate was significantly higher among patients who received high-dose therapy(51.3% vs 25.0%, p <0.05). Conclusions : In relapsed or refractory NHL, CDME chemotherapy is an effective salvage chemotherapy and allow peripheral blood stem cell collection. Also, high-dose chemotherapy with APBSCT following CDME is superior to CDME salvage chemotherapy only.

준설수심에 따른 사천만의 해수유동 특성에 관한 연구

강대석,유상호,민일규,이동수,민병형 東亞大學校 海洋資源硏究所 2003 硏究論文集 Vol.15 No.-

This study on the characteristics of the tidal current at the half closed bay selected Sachoˇn Bay located in the western Kyung-nam area as the object location of the study. Then, I analyzed the current with the varying dredging depth after the depth integral of the three Dimensional continuous equation and Navier-stokes equation. The study would get the following results, and the results could be used meamingfully as the analysis material about the dredging depth's influnce on the surrounding ocean when developing the coastal port. 1. When measuring the tidal current, there is a bit dispersion in the current direction of the observed object, but it is the wind effect at the bay. Considering that the calculated result is the shallow-water equation integranted in depth, the results can be concluded to in accored with my expectancy. 2. In case of reclaiming and dredging, the flow velocity and direciton change constantly, compared with the present condition. 3. At the wide bay dredge depth does not arrect the level of the ocean, but in case of inflow from the flood, the increase of the inflow of the river water affects the level of the ocean. 4. As the dredge depth grows deep, the maximum flood tidal current and obb tidal current at the front coast of the reclaimed decreases a little. However, the wide area Pc-7~Pc-9 doesn't show any change. As we saw in the above result, feacturcs of the tidal current as the level of the ocean and the current velocity don't change in the aspect of the ocean engineering even if we increase the degree of the dredge depth up to 6m in Sachoˇn Bay

양자교환 방식에 의한 방향성 결합기의 제작 및 특성연구

소대화,채기병,강기성,윤형도 明知大學校 産業技術硏究所 1992 産業技術硏究所論文集 Vol.11 No.-

In this study, a directional coupler which is fabricated on X-cut LiNbO₃ substrate by using proton exchange method is described. It is found that the directional coupler waveguide varied widely with controlling diffusion process. On the other hand, the on-off state of modulator is performed by annealing process and self-aligned electrodes are used in fabricating the direction coupler. As a results, the direction coupler has good characteristics : the measured on-off switching voltage of about 2.7-3V.

컴퓨터과학 교육을 중심으로 한 중등 컴퓨팅 교육과정 설계

신상국,권대용,김형신,염용철,유승욱,이원규 한국컴퓨터교육학회 2005 컴퓨터교육학회 논문지 Vol.8 No.3

포스트-텐션 프리캐스트 외부 넓은 보-기둥 접합부의 비탄성 거동

박상욱,최윤철,김영찬,김대중,임재형,권기혁,문정호,이리형 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2(구조계)

Experimental results from the cycle lateral load tests for post-tensioned precast wide beam-column connections were presented. Six exterior beam-column assemblages of half scale were tested in the laboratory. The specimens consist of U-shaped precast wide beams and concrete columns. The continuity at the beam-column joint is achieved with the topping concrete and post-tensioning. The specimens were designed with parameters of the beam-to-column depth ration and the number of tendons within column core. The test results showed that the post-tensioned precast wide beam-column connection satisfied the provisional standard by ACI of acceptance criteria for concrete special moment frames.