비소세포 폐암종에서 Cyclin D1, p21 및 p53단백의 발현과 예후와의 관계

양석우,조상호,양우익,정우희,안철민,이두연 대한병리학회 1999 Journal of Pathology and Translational Medicine Vol.33 No.12

최근 5년간 약진으로 입원한 환자의 고찰 - 임상양상 및 검사소견(1986∼1990) -

고우석,조광현,윤재일 大韓臨床藥理學會 1993 臨床藥理學會誌 Vol.1 No.1

구강에 발생한 편평상피암종의 MMP-2, MMP-9, TIMP-1, TIMP-2 발현과 EGFR 및 E-cadherin 발현의 상호연관성

조성재,권창석,김용환,윤혜경,김우형,이희철 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.4

Objective : Matrix metalloproteinases(MMPs) are involved in the degradation of extracellular matrix, which is related to infiltrative growth and metastasis of tumor. Positive correlation between MMPs expression and clinico-pathologic parameters were reported, and MMPs expressions were regulated by tissue inhibitor of metalloproteinases(TIMPs) or cell adhesion molecules such as E-cadherin and epidermal growth factor receptor EGFR). The aim of this study is to evaluate the relationship between MMP-2, MMP-9 expressions and clinico-pathologic factors such as clinical stage. recurrence. histologic grade and invasion pattern, and between MMP-2 and MMP-9 Expressions and TIMP-1, TIMP-2, and E-cadherin expressions. Methods and Material : The materials were 55 cases of squamous cell carcinoma of the oral cavity, and immunohisdtochemical studies for MMP-2, MMP-9, TIMP-1, TIMP-2, E-cadherin and EGFR were performed. Clinico-pathologic factors such as clinical stage, recurrence, histologic grade and invasion pattern were recorded through microscopic examination and clinical record review. Statistical analyses between MMP-2, MMP-9 expressions and clinico-pathologic factors, TIMP-1, TIMP-2 expressions and EGFR and E-cadherin expressions were done. Results : 1. MMP-2 and MMP-9 expressions were noted in 30(54.5%) and 22(40.0%) of 55 cases, respectively. TIMP-1 and TIMP-2 expressions were observed in 21(38.2%) and 33(60.0%), and E-cadherin and EGFR expressions were found in 35(63.6%) and 26(47.3%) of 55 cases, respectively. 2. MMP-2 expression rate was slightly higher in cases without recurrence, and TIMP-2 expression rate was slightly higher in cases showing more infiltrative growth pattern. However, there were no significant differences of MMP-2, MMP-9, TIMP-1, and TIMP-2 expression rates according to other clinico-pathologic parameters were noted. 3. The expression rate of EGFR was higher in cases with well differentiation (p=0.0047), but no positive relationship between the expression rate of E-cadherin and histologic grade was found. There were no significant differences of the expression rates of E-cadherin and EGFR according to other clinico-pathologic parameters. 4. Cases with positive reaction for MMP-9 showed an increasing tendency of negative reaction for TIMP-1. However, signigicant relationships between MMP-2, MMP-9 expressions and TIMP-1, TIMP-2 expressions were not noted. 5. The expression rate of MMP-2 was higher in cases with positive reaction for Ecadherin and EGFR with no statistical significance. The expression rate of MMP-9 was significantly higher in cases with positive reaction for E-cadherin(p=0.0221), but there was no significant relationship between MMP-9 and EGFR expression. Conclusion : These results suggest that MMP-2, MMP-9, TIMP-1 and TIMP-2 expressions are involved in the development of oral squamos cell carcinomas, but MMP-2, MMP-9, TIMP-1, and TIMP-2 expressions might not seem to be a sueful prognostic factors because there were no significant relationship between clinicopathologic parameters, EGFR expression showed positive correlation with low histologic grace, so EGFR expression could be regarded as a good prognostic factor. In the progression of squamous cell carcinoma of oral cavity, MMP-9 and E-cadherin might be involved with positive correlation, but there were no significant correlations between MMP-2, MMP-9 and TIMP-1, TIMP-2 and EGFR expressions rate. Further study for other factors such as cytokines or growth factors except TIMP-1, TIMP-2 and EGFR will be needed.

Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.

성인 급성 골수성 백혈병에서 6-Thioguanine, Cytarabine, Doxorubicin(TAD) 3제 복합화학요법의 효과 : 최종보고

최환석,윤휘중,우인숙,박지훈,김정희,김시영,조경삼 大韓血液學會 1995 大韓血液學會誌 Vol.30 No.2

환자만족도 조사설문지의 설문문항 배열에 따른 신뢰도 분석

최귀선,조우현,홍재석,이선희,강명근 한국의료QA학회 2000 한국의료질향상학회지 Vol.7 No.2

Objectives : The purpose of this study was to evaluate the reliability of questionnaire according to item arrangement on patient satisfaction questionnaire. Methods : We developed the two types of questionnaire with different item arrangement. In the first type (A), questions were arranged according to medical service dimensions. Questions in the second type (B) were arranged according to medical process. Both questionnaires were composed of six dimensions: physical environments, process, competence, courtesy, information giving, understanding patients. Measurements were performed on a 5-score Likert scale. In an outpatients and inpatient survey, total 777 patients answered the type A (outpatients: 257, inpatients: 128) or Type B (outpatients: 257, inpatients: 135). In order to compare the internal consistency of two types. Cronbach's α were calculated. Multiple regression analysis was also performed to know which type of questionnaire explain more of the overall satisfaction. Results : In outpatient survey, type A questionnaire showed higher internal consistency than B except physical environments dimension. Also in inpatient survey, type A had higer internal consistency than B in four dimensions(process, competence, courtesy, understanding patients). In the results of multiple regression analysis, type A questionnaire (R² =0.53) explained more of the variation in overall satisfaction then B questionnaire (R2=0.43) in outpatient survey. In inpatient survey, type B questionnaire (R²=0.40) explained, more of the variation in overall satisfaction than type A questionnaire (R² =0.33).But the.difference of R was not significant in inpatient survey. Conclusion : The results of this study support that type A questionnaire has higer reliability in assessment of consumer satisfaction than type B.

국내에서 분리된 Esherichia Coli 와 Klebsiella pneumoniae의 CTX-M형 Extended-Spectrum β-Lactamase 생성 현황

배일권,우건조,정석훈,박광옥,조병규,김둘만,권수봉,김현주,강현경 대한임상미샐물학회 2004 Annals of clinical microbiology Vol.7 No.1

태권도 우수선수들의 정신력 특성

최상진,조옥성,백우석,이갑철,고봉수 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.2

The purpose of this study was to investigate the mental toughness of Taekwondo players and to suggest way of performance enhancement using Loehr's Psychological Performance Inventory. Subjects were 188 Taekwondo elite players. Each factor's mean and standard deviation of Mental Toughness were calculated, and ANOVA were used for statistical analyses of the difference of mean. The results are as follow;: 1. On the level of self-confidence, it shows that the man players is higher than the woman players (school career F=5.846, P<.05: sex distinction F=8.876, P<.01). 2. On the level of attention control, it shows that the woman players is higher than the man players (school career F=4.483, P<.05: sex distinction F=9.814, P<.01). 3. On the level of visual and imagery control, it shows that the man players is higher than the woman players (school career F=7.790, P<.01: sex distinction F=5.000. P<.05). 4. On the level of arousal control, motivation, positive energy and attitude control, it was not significant difference according to the sexual and school career.

학교폭력 피해자의 정신병 실태 조사

신성웅,권석우,신민섭,조수철 대한소아청소년정신의학회 2000 소아청소년정신의학 Vol.11 No.1

요 약 : 본 연구는 학교폭력의 피해자를 대상으로 정신병리에 대한 특성을 알아내고, 조기 정신병과의 관계를 규명함을 기본 목적으로 시행되었다. 더 나아가서는 조기정신병의 발견과 아울러, 조기 치료를 시행함으로써 심각한 정신병의 예방을 도모하고자 하였다. 서울시 전 구역 중 16개 중학교를 선정하여 중학교 1, 2학년 남 · 녀 학생에게 간이정신진단검사 피어스-해리스 자아상 검사의 인기도와 학교 적응 소척도, 저자들이 개발한 따돌림 척도를 배포하여 따돌림을 당하는 정도의 점수가 평균보다 통계적으로 유의하게 차이가 나는 대상을 선정하고, 이들 중에서 스스로 인기가 없다고 여기면서 정신병적 증상이 유의하게 높게 보고된 대상을 선정하여 '정신병의 전구기에 있는 군'으로 판정하여, 이들의 대상의 수를 계산하여 위험도를 산출하고, 정신병 전구기에 있다고 판단되는 대상의 임상적 특성을 상관 분석 및 회귀 분석을 통해 정신병적 증상과 관련된 임상적 척도들을 파악하였다. 결과는 다음과 같다. 1) 따돌림 척도에서 통계적으로 유의하게 높은 점수를 받은 군은 총 157명(5.3%)이었고, 그 중에서 47명(29.9%)이 간이정신진단검사의 정신병적 증상 수치가 유의하게 높았다. 따돌림 척도에서 높지 않은 점수를 보인 2,135명 중 정신병적 증상이 높은 군은 50명이어서,따돌림 척도에서 높게 나온 군의 정신병적 증상이 높게 나올 위험도(odds ratio)는 17.8이었다 이는 통계적으로 유의한 수치였다(X2=274.8, df=1 p<0.05). 즉 따돌림을 당한다고 호소한 집단은 정신병적 증상이 유의하게 높았고, 이를 통해 왕따 피해자는 정신병 발병 위험이 통계적으로 유의하게 높다고 할 수 있었다. 2) 자신은 남들에겐 인기도 없고, 따돌림을 당한다고 여기며 정신병적 증상을 가진 군(47명, 남자31명, 여자 16명)은 단순히 정신병적 증상이 높은 군에 비해 불안 척도,우울증 척도, 공격성, 대인 민감성 학교 및 사회 적응 척도, 강박증상 척도, 공포불안 척도, 정신병적 증상 신체적 증상 호소 등에서는 차이를 보이지 않았으나 자신이 따돌림을 받는다는 호소와 편집증은 더 높게 측정되었다. 그리고, 학교폭력 피해자 중에서 정신병적 증상이 유의하게 높게 측정되었고, 스스로 인기가 없다고 생각하는 대상들에서는, 불안 증상, 우울증, 공격성, 대인 예민성, 강박증상, 편집증, 신체 증상 호소 등이 정신병적 증상을 잘 설명해주었다(r2=0.93). 즉, 따돌림을 당한다고 호소한 집단은 정신병적 증상이 유의하게 높았고, 이를 통해 왕따 피해자는 정신병 발병 위험이 통계적으로 유의하게 높다고 할 수 있었다. 이를 통해서 따돌림과 같은 학교폭력 피해자가 불안 증상, 우울증, 공격성, 대인 예민성, 강박증상, 편집증, 신체 증상 호소와 같은 문제를 보였을 경우에는 정신병적 증상 유무와 학교 적응, 집중력 장애, 사고개념 형성 장애 등을 확인하여 정신병의 진단을 고려해야 한다고 할 수 있다. Objectives : The primary purpose of this study is to understand the psychopathology of the victims of school violence in terms of early psychosis. By doing this, the early detection of psychosis among the victims is possible, and early detection may lead to early intervention. Methods : Two-thousand and nine-hundred seventy two students from 16 middle schools in Seoul were asked to fill out questionnaire comprised of popularity and intellectual and school status of Piers-Harris Children's Self Concept Inventory, Symptom Check List-90-Revised, and Ostracism Scale. The subjects whose scores upon Ostracism Scale were higher than average by two standard deviation were labeled as 'Repelled and Isolated group', and subjects whose scores on popularity were significantly lower than average and whose scores on psychoticism of SCL-90-R were higher than average were defined as 'tentative early psychosis group'. Odds ratios were calculated from the numbers of subjects with and without high psychoticism scores and high ostracism scores. On the subjects of 'tentative early psychotic group', we examined every clinical characteristic and conducted correlation analysis and regression analysis in order to find out the risk factors and to construct theoretical model that explains the psychoticism scores. Results : The results were a follows : 1)Total 157(5.3%) subjects were rated significatntly higher on ostracism scale, and among them, 47 subjects(29.9%) were rated significantly higher than average on psychoticism scale, while only 50 subjects among 2,135 students who were rated within normal range showed significantly higher score on psychoticism scale. Odds ratio for psychotic group of isolated group were 17.82 and it was statistically significant. 2)Forty-seven subjects(31 boys, 16 girls) who were rated as they were unpopular and rejected from peers had significantly higher psychoticism scores. They were not significantly different from simply high psychoticism subjects in anxiety, social anhedonia scale, magical thinking, obsessive-compulsive symptoms, phobic anxiety, psychoticism, somatization, but showed higher ostracism scores and paranoid tendencies. Among school violence victims, who rated themselves unpopular and showed higher psychoticism scores, the psychoticism scores were mainly explained by anxiety, depression, hostility, interpersonal sensitivity, obsessive-compulsive symptoms, paranoid tendency, somatization scales(r2=0.93). Conclusion : Thus, it can be concluded that the subjects with higher ostracism score have the substantially high risk for psychosis development. By these results, we propose that school violence victims with anxiety, depression, hostility, interpersonal sensitivity, obsessive-compulsive symptoms, paranoid tendency, somatization should be tested individually considering school adjustment, attentional deficit, concept formation problems. KEY WORDS : School violence·Ostracism·Psychosis.

소아에서 발생한 골수이형성 증후군 2례

홍성진,윤석중,김성우,조형구,오명호,김기혁,이인성 건국대학교 의과학연구소 1996 건국의과학학술지 Vol.5 No.-

Myelodysplastic syndrome(MDS) is a group of acquired conditions characterized by progressive bone marrow failure associated with normocellular or hypercellular bone marrow that cannot be attributed to nutritional deficiency, chronic infection, or other chronic systemic illness, and cannot be reversed by the successful treatment of those conditions. Whereas the cases of secondary MDS occur at all ages, primary MDS is essentially a disease of elderly, though no age group is exempt and predomonant in men. The MDS is fundamentally clonal disorders at the level of the haemopoietic totipotent stem cell. Clinically, they are characterized by varing degrees of peripheral cytopenia(s) with morphological and functional abnormalities of blood elements. We have experienced two unrelated pediatric cases, who showed pancytopenia on admission and the characteristic marrow findings of MDS. The first case, a 12 months-old girl, showed increased reticulin with abnormal localization of immature precursor cells(ALIP) in her bone marrow, in addition to dysplastic features such as bi-and multi-nucleation of late normoblasts and internuclear bridging. She also showed 22% of blasts in her bone marrow cells thus classified in the category of RAEB-t after FAB classification. Also found were a chromosomal abnormality namely 46 XX t(14q21q), which was an unusual one. Her family brought her home and, 4 month later, she deceased. The second case, a 12 year-old boy, showed pancytopenia with Pseudo-Pelger-Huet anomaly in neutrophils but no blasts in the peripheral blood. Bone marrow of this case also showed the picture of dyserythropoiesis with binuclearity, internuclear bridging, nuclear fragments, multinuclearity of late normoblasts, in addition to megaloblastoid change in the normoblasts and increased mitosis in granulocytic series. No chromosomal abnormality was found. Classified as RA after FAB classification of MDS. Showed good response to conservative management for anemia for more than one year.