PREVENTIVE EFFECT OF ADHESIVE TAPE SUPPLEMENTED WITH NaF ON ENAMEL EROSION IN VITRO

이상호,이난영,이인화,Lee, Sang-Ho,Lee, Nan-Young,Lee, In-Hwa Korean Academy of Pediatric Dentistry 2010 大韓小兒齒科學會誌 Vol.37 No.1

본 연구는 불소함유 접착테잎의 법랑질 침식 예방효과를 평가하기 위해건강한 소의 치아를 절단하여 아크릴 주형에 매몰하고 정중선에 홈을 형성하여 대조면과 불소 제제를 도포할 실혐면으로 나누었다. 시편을 무작위로 18개씩 4군으로 나누었다. 1군은 APF gel를 도포하고, 2군은 불소 바니쉬를 도포하였으며, 3군과 4군은 5% NaF를 각각 첨가한 methyl cellulose와 poly vinyl alcohol 테잎을 부착하였다. 시편을 콜라에 5분, 증류수에 10분씩 6회 번갈아 처리하며 5일 동안 반복하였다. 시간 경과에 따라 표면미세경도를 측정하고 5일후 각 시편을 절단하여 침식병소의 깊이를 측정하여 다음과 같은 결과를 얻었다. 1. 표면미세경도 값은 5일째만 제외하고 2군, 3군, 4군의 실험면이 각각의 대조면에 비해 컸다 (p<0.05). 2군, 3군, 4군의 실험면 역시 5일째만 제외하고 1군의 실험면에 비해 표면미세경도가 컸다 (p<0.05). 3군과 4군 사이에는 표면미세 경도의 유의한 차이가 없었다(p>0.05). 1군의 실험면과 대조면은 표면미세경도의 유의차가 없었다(p>0.05). 2. 침식병소의 깊이는 2군, 3군, 4군의 실험면이 각각의 대조면에 비해 컸다(p<0.05). 2군, 3군, 4군의 실험면은 1군의 실험면에 비해 침식병소의 깊이가 얕았다 (p<0.05). 2군, 3군, 4군의 실험면 사이의 침식병소의 깊이는 유의차가 없었다(p>0.05). 상기 결과로 보아 새로 개발된 불소함유 접착테잎은 기존의 불소 바니쉬와 같은 법랑질 침식 예방효과가 분명하게 나타나, 법랑질 침식에 감수성이 높은 어린이 환자에게 임상적인 예방법으로서 활용할 수 있는 가능성이 높다고 사료된다. This study examined the effect of adhesive tape supplemented with sodium fluoride on the prevention of dental erosion in vitro. Sound bovine tooth samples were selected and divided randomly into the following 4 groups according to the material treatments: group 1, APF gel; group 2, fluoride varnish; and groups 3 and 4, fluoride tape supplemented with 5% NaF in either a methyl cellulose or poly vinyl acetate carrier, respectively. All specimens were submitted to alternate cycles of acid exposure in a cola beverage (pH 4.3) and artificial saliva for $6\;{\times}\;5\;min/day$ over a 5 day period. The micro-hardness was recorded each day and the lesion depth was measured after 5 days. The micro-hardness of the experimental sides of groups 2, 3 and 4 were significantly higher than that of their control sides and the experimental side of group 1 during the experimental period (p<0.05) except on the 5th day. The enamel surfaces of treatment groups 2, 3 and 4 showed significantly higher resistance to mineral loss in terms of the erosion depth (p<0.05) than group 1 and their control sides. There was no statistically significant difference among group 2, 3 and 4, indicating that the fluoride varnish and tapes produce similar results. Fluoride adhesive tapes are effective in reducing the progression of erosion and can be recommended for young patients who are more susceptible to dental erosion.

중풍환자(中風患者)의 불면증(不眠症)에 피내침(皮內針)의 유효성(有效性) 및 적응증(適應證) 평가(評價)

이상호,김은주,김이동,윤상필,이차로,홍진우,정동원,문상관,조기호,김영석,Lee Sang-Ho,Kim Eun-Ju,Kim Lee-Dong,Yun Sang-Pil,Lee Cha-Ro,Hong Jin-Woo,Jeong Dong-Won,Moon Sang-Kwan,Cho Ki-Ho,Kim Young-Suk 대한한방내과학회 2004 大韓韓方內科學會誌 Vol.25 No.1

Objective : Insomnia is the disorder of initiation or maintenance in sleeping that results in daytime sleepiness and dysfunction, and it arises from multiple psychological, physiological and environmental factors. A number of stroke patients suffer from insomnia are classified as sleep disorder patients with physical illness. In addition, insomnia may have profound deleterious effects on the natural course of stroke. We are to assess the effectiveness of intradermal acupucture on stroke combined with insomnia. Methods : We recruited hospitalized patients with insomnia after stroke. Then, the subjects were allocated into a treatment group and a control group by randomization. The treatment group received intradermal acupuncture therapy at He-7(神門), EH-6(內關) correctly. However in control group, intradermal acupuncture were just attached to He-7(神門), EH-6(內關) without insertion. Treatment over time at first day and second day a various indexes were repeatedly checked such as sleep latency, total sleep time, sleep quality, condition on awakening, ability of concentration, morning sleepiness, Insomnia Severity Index, and Athens Insomnia Scale. In the second analysis, we divided the treatment group into a response and a non-response group by their response to intradermal acupuncture. With the acupuncture treatment, accompanied symptoms were checked Results : Among thirty two subjects, two of them were dropped out: One complained the pain from needle insertion, and the other underwent the change of his herbal medication. At baseline investigation with the residual 30 subjects, the control and the treatment group were assessed to have an equal comparability. In the treatment group, sleep latency, total sleeping time, a number of awakenings, sleep quality and ability of concentration showed improvement significantly compared with the control group. The non-response Group showed symptoms such as nausea and halitosis, belching and acid regurgitation, abundant expectoration, while Responder Group showed palpitation, oppressed feelings in chest and sleep disorder. Conclusions: The result of this clinical study suggests that the intradermal acupuncture at He-7(神門), EH-6(內關) is one of the effective treatments for the insomnia after stroke.

이어도 종합해양과학기지의 복사관측소 환경 개선 및 국제화 추진 전략

이상호,조일성,이규태,김부요,정현석,임세훈,변도성,이주영,LEE, SANG-HO,Zo, Il-SUNG,LEE, KYU-TAE,KIM, BU-YO,JUNG, HYUN-SEOK,RIM, SE-HUN,BYUN, DO-SEONG,LEE, JU-YEONG 한국해양학회 2017 바다 Vol.22 No.3

정확한 복사 관측자료는 기후 및 날씨뿐만 아니라 해양과 건축 및 농 축산 분야 등 중요하게 활용된다. 특히 이어도와 같은 종합 해양과학기지는 주변 환경에 의한 복사 반사 효과를 최소화할 수 있기 때문에 이상적인 관측소로 평가되며 여기서 생산되는 자료는 복사관측의 기준 자료가 될 수 있다. 또한 BSRN (Baseline Surface Radiation Network) 등 세계복사관측그룹 가입을 위한 필수 장비들(전천과 직달 및 산란 일사계 등)을 갖추고 관측소 환경을 개선할 경우 전 세계적인 중요 관측소로 부각될 수 있는 여건을 갖추고 있다. 이어도 종합해양과학기지는 2004년 11월부터 일사계 관측을 수행해 왔고 이 연구에서는 2005년 1월 1일부터 2015년 12월 31일까지의 자료를 분석하였다. 이 연구 기간에 이어도의 일평균 일사량은 $-3.80W/m^2/year$로 감소하였고 그 이유는 자연적 원인 이외에 관측장비의 센서 반응도 감소 효과가 포함되어 있기 때문이다. 즉 이어도 종합해양과학기지의 경우 정밀한 복사 관측 자료를 생산하기 위해서는 관측소 환경 개선뿐만 아니라 관측장비 및 자료 보정이 요구된다. 그리고 한반도의 대기 청정도와 국민 건강을 크게 위협하고 있는 중국발 황사와 미세 먼지는 전 세계 과학자들의 중요 관심 대상이기 때문에 이어도뿐만 아니라 신안 가거초 및 옹진 소청초 종합해양과학기지 복사 관측자료를 통한 공동 대응 근거 마련이 필요하며 그에 따라 이들 복사관측소 환경 및 장비 개선 그리고 관측자료 정확성 향상 계획 수립이 시급하다. The radiation observation data will be used importantly in research field such as climatology, weather, architecture, agro-livestock and marine science. The Ieodo Ocean Research Station (IORS) is regarded as an ideal observatory because its location can minimize the solar radiation reflection from the surrounding background and also the data produced here can serve as a reference data for radiation observation. This station has the potential to emerge as a significant observatory and join a global radiation observation group such as the Baseline Surface Radiation Network (BSRN), if the surrounding of observatory is improved and be equipped with the essential radiation measuring instruments (pyaranometer and pyrheliometer). IORS has observed the solar radiation using a pyranometer since November 2004 and the data from January 1, 2005 to December 31, 2015 were analyzed in this study. During the period of this study, the daily mean solar radiation observed from IORS decreased to $-3.80W/m^2/year$ due to the variation of the sensor response in addition to the natural environment. Since the yellow sand and fine dust from China are of great interest to scientists around the world, it is necessary to establish a basis of global joint response through the radiation data obtained at the Ieodo as well as at Sinan Gageocho and Ongjin Socheongcho Ocean Research Station. So it is an urgent need to improve the observatory surrounding and the accuracy of the observed data.

반도체 습식 세정 공정 중 상온의 초순수와 염기성 수용액 내에서 오존의 용해도 최적화

이상호,이승호,김규채,권태영,박진구,배소익,이건호,김인정,Lee Sang-Ho,Lee Seung-Ho,Kim Kyu-Chae,Kwon Tae-Young,Park Jin-Goo,Bae So-Ik,Lee Gun-Ho,Kim In-Jung 한국반도체디스플레이기술학회 2005 반도체디스플레이기술학회지 Vol.4 No.4

The process optimization of ozone concentration and half life time was investigated in ultra pure water and alkaline solutions for the wet cleaning of silicon wafer surface at room temperature. In the ultra pure water,. the maximum concentration (35 ppm) of ozone was measured at oxygen flow rate of 3 liters/min and ozone generator power over 60%. The half life time of ozone increased at lower power of ozone generator. Additive gases such as $N_2$ and $CO_2$ were added to increase the concentration and half life time of ozone. Although the maximum ozone concentration was higher with the addition of $N_2$ gas, a longer half life time was observed with the addition of $CO_2$. When $NH_4OH$ of 0.05 or 0.10 vol% was added in DI water, the pH of the solution was around 10. The addition of ozone resulted in the half life time less than 1 min. In order to maintain high pH and ozone concentration, ozone was continuously supplied in 0.05 vol% ammonia solutions. 3 ppm of ozone was dissolved in ammonia solutions. The static contact angle of silicon wafer surface became hydrophilic. The particle removal was possible alkaline ozone solutions. The organic contamination can be removed by ozonated ultra pure water and then alkaline solution containing ozone can remove the particles on silicon surface at room temperature.

일산화탄소 단기 노출에 따른 순환계통 질환 위험과 진료비용 예측을 위한 IoT 활용 방안

이상호,조광문,Lee, Sangho,Cho, Kwangmoon 한국사물인터넷학회 2020 한국사물인터넷학회 논문지 Vol.6 No.4

본 연구는 2010년 1월부터 2018년 12월까지 일산화탄소의 12일 단기 노출에 따른 순환계통 질환 사망자 수의 영향관계를 분석하였고, 일산화탄소 농도 증가에 따른 미래의 순환계통 질환의 진료비용을 예측하였다. 한국환경공단의 대기환경정보(Airkorea)와 한국 통계청에서 자료를 추출하였고, 포아송 회귀분석과 ARIMA 개입 모형을 사용하여 분석하였다. 통계처리는 SPSS Ver. 21.0 프로그램을 이용하였다. 연구 결과는 다음과 같다. 첫째, 일산화탄소의 단기 노출에 따른 순환계통 질환 사망에 영향관계를 당일부터 이전 11일 전까지 분석한 결과는 이전 11일에서 가장 높은 영향력이 있는 것으로 나타났다. 둘째, 일산화탄소 농도 증가에 따라 미래의 순환계통 질환 진료비용은 2019년 예측값이 10,123십억원으로 2018년 12월 말의 관측값 9,443십억원보다 높게 나타났다. 또한 월별로 정리해 보면 순환계통 질환 진료비용은 계절변동이 반영되어 1월 보다 12월로 갈수록 높아진다는 것을 알 수 있었다. 이러한 연구를 통하여 일산화탄소와 같은 대기오염물질 증가에 따른 선제적 대응을 위하여 IoT를 활용한 다양한 기기 및 장비를 보급함으로써 모든 국민의 건강한 삶을 위한 미래가 실현될 수 있을 것이다. This study analyzed the effect of the number of deaths of circulatory system diseases according to 12-day short-term exposure of carbon monoxide from January 2010 to December 2018, and predicted the future treatment cost of circulatory system diseases according to increased carbon monoxide concentration. Data were extracted from Air Korea of Korea Environment Corporation and Korea Statistical Office, and analyzed using Poisson regression analysis and ARIMA intervention model. For statistical processing, SPSS Ver. 21.0 program was used. The results of the study are as follows. First, as a result of analyzing the relationship between the impact of short-term carbon monoxide exposure on death of circulatory system diseases from the day to the previous 11 days, it was found that the previous 11 days had the highest impact. Second, with the increase in carbon monoxide concentration, the future circulatory system disease treatment cost was estimated at 10,123 billion won in 2019, higher than the observed value of 9,443 billion won at the end of December 2018. In addition, when summarized by month, it can be seen that the cost of treatment for circulatory diseases increases from January to December, reflecting seasonal fluctuations. Through such research, the future for a healthy life for all citizens can be realized by distributing various devices and equipment utilizing IoT to preemptively respond to the increase in air pollutants such as carbon monoxide.

비대면 시대에 IoT를 활용한 청년 구직활동수당 지원사업 접근 방안 모색

이상호,조광문,Lee, Sangho,Cho, Kwangmoon 한국사물인터넷학회 2020 한국사물인터넷학회 논문지 Vol.6 No.3

The purpose of this paper is to verify the differences of the effect of the support between the number of employment of those who have experienced employment and the unemployment period of those who have not experienced employment for the participants of the Jeollanam-do Youth Job Search Allowance Support Project. In addition, this study attempted to find out the ways to develop the system using the Internet of Things by figure out how to use the allowance, the field of help, the average investment time per day, the average monthly expenditure, the difficulties of job search activities, and the differences in the youth job search activities for the required programs. In this study, 602 people who participated in the Jeollanam-do Youth Job Search Allowance Support Project from February 19 to March 9, 2020 are surveyed. The collected data was processed using the SPSS 21.0 program. The research results are as follows. First, there was a difference in satisfaction according to the number of jobs employed. Second, there was a difference in the effectiveness according to the number of employment. Third, there was a difference in satisfaction according to the period of unemployment. Fourth, there was a difference in effectiveness according to the period of unemployment. In order to enhance the effectiveness of the youth job search allowance support project, the Edutech industry and technology that can share various information using the Internet of Things environment in the untact era must be improved together. 본 논문은 전라남도 청년 구직활동수당 지원사업의 참여자를 대상으로 취업을 경험한 대상자의 취업 횟수와 취업을 경험하지 못한 대상자의 실업 기간에 따른 지원 효과에 대한 차이를 검증하고자 하는 것이다. 또한 수당 사용방법, 도움 분야, 하루 평균 투자시간, 월평균 지출 비용, 구직활동의 어려운 점, 요구되는 프로그램의 청년 구직활동의 차이를 파악하여 사물인터넷을 활용한 제도의 발전 방안을 모색하고자 하였다. 본 연구는 2020년 2월 19일부터 3월 9일까지 전라남도 청년 구직활동수당 지원사업에 참여한 대상자 602명이 설문조사에 참여하였다. 수집된 자료는 SPSS 21.0 프로그램을 활용하여 자료를 처리하였다. 연구결과는 다음과 같다. 첫째, 취업 횟수에 따른 만족도는 차이가 있는 것으로 나타났다. 둘째, 취업 횟수에 따른 효과성은 차이가 있는 것으로 나타났다. 셋째, 실업기간에 따른 만족도는 차이는 있는 것으로 나타났다. 넷째, 실업기간에 따른 효과성은 차이가 있는 것으로 나타났다. 청년 구직활동수당 지원사업의 효과를 제고하기 위해서는 비대면 시대에 사물인터넷 환경을 활용하여 다양한 정보를 공유할 수 있는 에듀테크 산업과 기술이 함께 개선되어야 할 것이다.

영리법인(주식회사) 병원 도입의 필요성

이상호,Lee, Sang-Ho 대한병원협회 2002 대한병원협회지 Vol.31 No.5

LPS로 유도된 마우스 대식세포주인 RAW264.7에서 MAPK 조절에 의한 백미 물추출물의 항염증 활성

이상호,유지현,길기정,Lee, Sang-Ho,Yoo, Ji-Hyun,Kil, Ki-Jung 대한본초학회 2022 大韓本草學會誌 Vol.37 No.6

Objectives : To develop natural ingredients that help prevent or treat anti-inflammatory-related diseases and use themas basic data, we investigated anti-inflammatory activity of Cynanchi Atrati Radix Et Rhizoma water extracts(CWE) in lipopolysaccharide(LPS)-induced murine macrophage cell line, RAW 264.7 cells. Methods : The cell viabilities were evaluated with RAW 264.7 cells. The production of nitric oxide(NO), prostaglandin E2(PGE2), pro-inflammatory cytokines such tumor necrotic factor(TNF)-α and interleukin(IL)-6 were assessed in LPS-induced RAW 264.7 cell treated with CWE. Furthermore, the protein expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2(COX-2), and mitogen-activated protein kinase(MAPK) were assessed by western blotting. Results : In RAW 264.7 cell, the cell viability by CWE treatment was more than 98.4% at a concentration of 100-400 ㎍/mL. At a concentration of 800 ug/ml of CWE, the cell viability was as low as 86%. At doses of 100, 200 and 400 ㎍/mL, CWE inhibited the production of NO, PGE2, TNF-𝛼 and IL-6 in a dose-dependent manner and also decreased the expression of iNOS and COX-2 from LPS-induced RAW 264.7 cells. In addition, CWE significantly inhibited the MAPK pathway including decreased the phosphorylation of the p38, c-Jun N-terminal kinase(JNK) and extracellular signal-regulated kinase(ERK1/2). Conclusions : Our study provides evidence that CWE inhibits the production of main pro-inflammatory molecules in LPS-induced RAW 264.7 cells via expression of p38, JNK, and ERK1/2 MAPK signaling pathways. Therefore, CWE is expected to be widely used as a natural ingredient for anti-inflammatory functional foods or pharmaceuticals in the future.

신장 피질 기저막 소포에서 p-Aminohippurate 이동의 특성에 대한 연구 -음이온의 효과-

이상호,정진섭,김용근,Lee, Sang-Ho,Chung, Jin-Sup,Kim, Yong-Keun 대한생리학회 1986 대한생리학회지 Vol.20 No.2

가토 근위세뇨관에서 Percoll gradient에 의해 분리한 기저막소포(basolateral membrane vesicle)에서 PAH 이동에 미치는 여러 음이온들의 영향을 rapid filtration technique을 이용하여 관찰하였다. NA-dependent PAH 축적은 용액내 $SO_4$와 $SSO_3$에 의하여 유의하게 억제되었으나 $Cl,\;SCN,\;HPO_4,\;acetate$ 및 oxalate에 의해서는 영향을 받지 않았다. 용액내의 $SO_4$는 상경적으로 PHA 이동을 억제하였다. 소포내 음이온을 부하시킨 후 PAH 이동을 측정했을 때 $acetate,\;SO_4$ 및 PAH의 부하에 의해 PAH 이동은 유의하게 증가되었으며, 소포내 부하되는 $SO_4$농도가 증가함에 따라 PAH 축적은 증가되었다. $SO_4$에 의한 PAH의 trans-stimulation은 Na 농도 경사 존재시 더욱 증가되었으며 이들은 2 mM probenecid 및 1mM SITS에 의해 억제되었다. 이들 결과들은 신피질세뇨관 세포의 기저막에 PAH/음이온 교환에 의해 PAH가 이동되는 기전이 존재한다는 것을 가르킨다. 그러나 PAH 이동에 영향이 없는 음이온들은 PAH/음이온 교환에 기질로써 작용하지 않기 때문인지 아니면 이들 음이온들의 높은 투과성에 인해 나타난 결과인지는 더욱 추구해 보아야 확인될 것으로 생각된다. Effects of anions on p-Aminohippurate (PAH) transport across the basolateral membrane (BLM) were studied. Basolateral membrane vesicles were isolated from rabbit renal cortex by using a self-orienting Percoll-gradient centrifugation and $Mg^{2+}-precipitation$. The characteristics of the membrane vesicles was examined by marker enzyme activity, membrane orientation and transport studies. The Na-K-ATPase activity in the fraction containing BLM vesicles was enriched 9·fold, and the alkaline phosphatase activity in the fraction containing BBM vesicles was increased 9-fold, compared with those of the homogenate. The transport properties of the two membrane preparations were studied by a rapid filtration technique. The uptake of PAH by BLM was sensitive to changes in medium osmolarity and inhibited by probenecid. When the uptake of $50{\mu}M$ PAH in voltage-clamped BLM vesicles was determined in the presence of various anions in the incubation medium, cis inhibitions by $SO_4\;and\;SSO_3$ were observed in the presence of sodium gradient (out>in). Sodium-dependent PAH uptake was inhibited competitively by external $SO_4$ PAH uptake in BLM vesicles loaded with 20 mM acetate and $SO_4\;or\;200\;{\mu}M$ PAH was significantly stimulated as compared with unloaded vesicles. The extent of trans-stmulation of PAH uptake by $SO_4$. was increased with the inside concentration of $SO_4$. This trans-stimulatory effect by $SO_4$, was observed to be additive in the presence of Na gradient and completely inhibited by 2 mM probenecid and 1 mM SITS. These results demonstrate that PAH/anion exchange is present in BLM of renal cortex and in this exchange mechanism inorganic as well as organic anions are involved as substrates.

Benzo(a)pyrene으로 유발한 Mouse Forestomach Tumor 생성에 대한 Astaxanthin 함유 난황의 효과

이상호,박철우,박원석,이영춘,최의성,하영래 ( Sang H . Lee,Cherl W . Park,Won S . Park,Young C . Lee,Eui S . Choi,Yeong L . Ha ) 한국응용생명화학회 1997 Applied Biological Chemistry (Appl Biol Chem) Vol.40 No.6

Anticarcinogenic activity of astaxanthin-containing egg yolks (designate AEY) was investigated for benzo[a]pyrene (BP)-induced mouse forestomach tumorigenesis initiating regimen. Female ICR mouse (6-7 weeks of age) were housed in polycarbonated cages (5 mice/cage; 20 mice/treatment) in a humidity-and-temperature-controlled facility and permitted free access to water and food. One week later, four and 2 days prior to p.o. treatment with BP (2 ㎎/0.2 ㎖ corn oil), mice were given 0.2 ㎖ PBS containing 50 ㎎ AEY, 100 ㎎ AEY, 150 ㎎ AEY, or 150 ㎎ CEY. Control mice were only given 0.2 ㎖ PBS. Three days later this sequence was repeated for a total of 4 times. Beginning with the first intubation and continuing thereafter, body weight and food intake were recorded once weekly. All surviving mice were sacrificed 24 weeks after the first dose of BP. Mice treated with AEY developed only about one third as many neoplasms/animal as mice in control or CEY-treated group (p$lt;0.05). Reduction effect of tumor development by AEY was dependent upon doses applied. Tumor incidence was also reduced by AEY treatments, but significantly reduced only by 150 ㎎ AEY treatment when compared to that by control or CEY. Food intake and body weight were not affected by AEY treatment. These results indicate that AEY inhibits tumorigenesis of mouse forestomach induced by BP.