패류중 중금속함량에 관한 연구

신현수,허옥순,이진하,김세은,주인선,신동우,강석종,박만종,김지연,김근희,강숙경,정래석,허숙진,소유섭,최장덕,문병우,김진수 식품의약품안전청 2001 식품의약품안전청 연보 Vol.5 No.-

본 연구는 패류중 중금속함량 측정을 위한 분석효율이 높고 간편한 분해법을 찾아내어 그 방법에 의끓 유종중인 패류중 중금속함량을 파악하고자 수행하였다. 시료를 분해시키는 Microwave와 습식, 건식회법을 비교한 결과 변형습식회화르가 가장 적합하였다. 변형습식회차딘는 려린0,3+C104+H2SOf의 혼찰산을 쪼 · j 1 (v/v)의 비율로 혼합하여 사용하였 을 때 회수을 Pb린틴. Cd 97.8, Cu 94.2, Zn 92.8%를 나타내었근 분해시간은 2~2.5시간 소요되었다. 유통중인 패류중 중금속함량 」최소~최대(평균), mgAgl 은 Pb 0.028~o.528(0.153), Cd 0.OO7~1,832(0.587), Cu 0.360~37.375(4.6691, Zn 6.317~233.2iO(38.881), Hg 0.001 ~O.151(0.012)mg/ltg으로 나타났다. 또한 유통중인 5종의 패류중 중금속평균항량이 생산지 하재지역에서 구입한 패류에 비해 모든 중금속언써 약간 높은 함량을 보였으나 오염된 수준이 아닌 자연함유량수준이었다. This study was conducted to improve the digestion method for the determination of heavy metals in shellfishes and to estimate the contents of heavy metal in commercial shellfishes by their digestion method. This digestion method was compared microwave with wet and dry ash digestion for shellfish samples, obtained results showed that the modified wet ash Ⅱ digestion was convenient and easy to use. For the modified wet ash Ⅱ digestion, The mixed acid of HNO₃+HClO₄+H₂SO₄(25:5:1, v/v) was used. Under this condition, Recoveries were Pb 94.3, Cd 97.8, Cu 94.2 and Zn 92.8% and digestion time was ∼2.5 hours. The contents of heavy metal [minimum∼maximum (mean), mg/kg in commercial shellfishes were as follows ; Pb:0.028∼0.528(0.153), Cd:0.007∼1.832(0.587), Cu:0.360∼37.375(4.669), Zn:6.317∼233.250(38.881), Hg:0.001∼0.151(0.013). The contents of heavy metal in commercial shellfishes of five species were slightly greater than those of shellfishes purchased at Hajae. There was no great difference between the contents of heavy metal of Hajae and those of shellfishes purchased at Whole market.

여성 외음부에 발생한 선상 편평상피세포암종

김창옥,양기화,강석진,이안희,김병기,김선무 대한병리학회 1991 Journal of Pathology and Translational Medicine Vol.25 No.1

Conceptual Study for Tissue-Regenerative Biodegradable Magnesium Implant Integrated with Nitric Oxide-Releasing Nanofi bers

Jin‑Kyung Jeon,Hyunseon Seo,Jimin Park,Soo Ji Son,Yeong Rim Kim,Eun Shil Kim,Jong Woong Park,Woong‑Gyo Jung,Hojeong Jeon,Yu‑Chan Kim,Hyun‑Kwang Seok,Jae Ho Shin,Myoung‑Ryul Ok 대한금속·재료학회 2019 METALS AND MATERIALS International Vol.25 No.4

The excessive initial corrosion rate of Mg is a critical limitation in the clinical application of biodegradable Mg implantsbecause the device loses its fi xation strength before the fractured bone heals. This study suggests a new approach to overcomethis hurdle by accelerating tissue regeneration instead of delaying the implant biodegradation. As angiogenesis is anessential process in early bone regeneration, a Mg implant coated with electrospun nanofi bers containing nitric oxide (NO),which physiologically promotes angiogenesis, is designed. The integrated device enables adjustable amounts of NO to bestored on the NO donor-conjugated nanofi ber coating, stably delivered, and released to the fractured bone tissue near theimplanted sites. An in vitro corrosion test reveals no adverse eff ect of the released NO on the corrosion behavior of the Mgimplant. Simultaneously, the optimal concentration level of NO released from the implant signifi cantly enhances tube networkformation of human umbilical vein endothelial cells without any cytotoxicity problem. This indicates that angiogenesis canbe accelerated by combining NO-releasing nanofi bers with a Mg implant. With its proven feasibility, the proposed approachcould be a novel solution for the initial stability problem of biodegradable Mg implants, leading to successful bone fi xation.

전기 영동법으로 증착한 형광체의 레이저 처리 특성연구

서도석,김채옥,홍진표 한양대학교 자연과학연구소 2001 自然科學論文集 Vol.20 No.-

효율적인 후처리 어닐링 방법으로 전기 영동법으로 제작한 형광제의 레이저 처리를 하였다. 소스로서는 연속발진 Nd:YAG레이저를 사용하였다. 실험적인 결과로서 형광제에 레이져 처리 후에는 형광제 효율에서 10% 증가를 가져 왔으며 매우 많은 시간에 효과적인 처리를 할 수 있었다. 이러한 효율의 증대는 형광제 표면에 존재하는 울눈물등의 표면처리에 기인하는 것으로 예상된다. Laser treatment of electrophoretically deposited phosphors has been carrid out as one of efficient post-annealing methods. A ND:YAG continuous wave(λ=1,064nm) laser was mainly used as an irradiation source. The experimental results presented useful laser-annealing time and enhanced improvement of about 10% in the luminance efficiency after the laser irradiation on the phosphor. The improvement in the luminescence was thought due to the surface cleaning effect on the phosphor

태권도 우수선수들의 정신력 특성

최상진,조옥성,백우석,이갑철,고봉수 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.2

The purpose of this study was to investigate the mental toughness of Taekwondo players and to suggest way of performance enhancement using Loehr's Psychological Performance Inventory. Subjects were 188 Taekwondo elite players. Each factor's mean and standard deviation of Mental Toughness were calculated, and ANOVA were used for statistical analyses of the difference of mean. The results are as follow;: 1. On the level of self-confidence, it shows that the man players is higher than the woman players (school career F=5.846, P<.05: sex distinction F=8.876, P<.01). 2. On the level of attention control, it shows that the woman players is higher than the man players (school career F=4.483, P<.05: sex distinction F=9.814, P<.01). 3. On the level of visual and imagery control, it shows that the man players is higher than the woman players (school career F=7.790, P<.01: sex distinction F=5.000. P<.05). 4. On the level of arousal control, motivation, positive energy and attitude control, it was not significant difference according to the sexual and school career.

갑상선 질환에서 혈청 Microsome 항체 측정의 의의

윤영옥,장순모,박호자,이준열,양기화,정인숙,강석진 대한임상병리사협회 1984 임상병리사회지 Vol.16 No.1

김천대학생들의 구취인지 실태에 관한 조사연구

이은숙 ; 김혜진 ; 김희천 ; 최옥선 김천대학교 2003 김천대학교 논문집 Vol.24 No.-

To help the guidance of treatment method, prevention of halitosis, and oral hygiene management for halitosis patient or non-halitosis group for investigating the status in accordance with awareness of halitosis to improve the oral health for college students, the survey including ques- tionnaire was performed for 331 persons of students who is studying in the Gimcheon college located in the northern province of Kyeongsang-Do. As a conclusion, the following data are obtained. 1. For the question of "I am acknowledging the halitosis", the replying person were 123 persons (37.2%), including men-69 persons(20.8%), women-54 persons(16.3%), So, in case of the ratio of acknowledging the halitosis, men was higher than women. On the contrary, for the question of "I do not acknowledging the halitosis", men-68 persons (20.5%), women-140 persons(42.3%), So, in case of the ratio of non-ackowledging the halitosis, men was much higher than women. 2. For the question of "When did you acknowledge the halitosis at most in the whole day?", total of 127 persons(38.4%) said "as soon as get up" out of the 183 persons (55%) who acknowledging the halitosis. The remaining replies were followed in the order of "before breakfast", "after breakfast", and "before sleeping". 3. In the status of oral hygiene, it showed that the persons who acknowledge the halitosis acts positively the frequency of toothbrushing (more than twice a day), method of toothbrushing, and brushing the tongue than the persons who do not acknowledge the halitosis. 4. In the status of smoking habits, there was no increment according to the duration of smoking, but in the status of amount of smoking, the persons who smokes less than half carton a day were 20 (6.0%), half through one carton a day were 31 (9.4%). So, it showed that the amount of smoking is affected to the cause of halitosis. 5. The ordinary prevention methods for oral hygiene according to the awareness of halitosis were investigated in the order of toothbrushing/brushing the tongue (37.3%), chewing the gum (33%), and using the rinsing liquids (12.4%).

개입연구를 통한 근로자 경견완 장애의 개선효과

손명호,손석준,박종,최진수,김양옥 大韓産業醫學會 1999 대한직업환경의학회지 Vol.11 No.4

Objectives : In order to reveal the effectiveness of the intervention in the workplace on patients suffering from cervicobrachial disorder(CBD), we reexamined 96 workers out of 137 workers suffering from CBD on the assembly lines of an electric manufacturing factory by using questionnaires, physical exams and neurological exams. Methods : Analyses were made of the characteristics of the 96 workers of this company suffering from 1994 to 1996 with special concern for differences before and after the improvement of the working environment. After reexamination of the 96 patients the following results were obtained. Results : We observed an improvement in the 10 most common subjective symptoms of CBD. These symptoms included general fatigue, pain on neck and shoulders, dullness of the upper extremities, back pain and discomfort and a tingling sensation in the hands and wrists(p<0.05). The percentage of workers who complained muscle tenderness of neck and shoulder were reduced(p<0.05). the percentage of workers with abnormal Morley examination was reduced from 28.5% to 2.1%(p<0.01), and of workers with abnormal EMG finding went from 12.4% to 2.1%(p<0.01) and of grade 3 and grade 4 CBD was reduced from 42.3% and 18.3% respectively to 33.3% and 2.1%(p<0.01). Grade 3 and over laborers of CBD had higher scores in somatization, anxiety, depression, hostility, interpersonal sensitivity, phobic anxiety, obsessive-compulsive, neurasthenic, hypochondriacal than other sufferers in the SCL-90-R exam(p<0.01). Conclusions : in conclusion intervention had effectiveness on subjective symptoms of neck and shoulder and decreasing abnormal finding of muscle tenderness, neurological examination and EMG.

수두백신의 역가시험법 표준화를 위한 연구

민경일,백선영,신진호,김재옥,류승렬,민복순,김병국,김도근,김훈,이석호,박순희 식품의약품안전청 2001 식품의약품안전청 연보 Vol.5 No.-

수두(chicltenpo즈) 및 대상포진(shingles)의 예방을 위해 사용되는 수두 생 바이러스 백근은 현재 국내 -외 제조사들이 정상사람 2배체 폐 태아 세포 주들을 이용하여 바이러스를 배양 · 증식시켜 생산하고 있으나, 품질관리를 쒸한 역가 시험에는 t·fRC튼 띤 LB딘EL 세포 주외에 제조사가 설정한 세포 주를 이용하고 있으며, 역파신험 방법인 fH Ui'rFO Plaque 3SSaIT에도 조사별로 다소 차이가 있는 시헌법을 사용하고 있다. 그러므로 일반적으로 세포 주간우 감수성 차이, 계대력(passa딩e level)간의 차이,varicelta-zoster virus(4'2V) diluent medium 등에 의한 차이에 의해 역가시힘 결과에 많은 차이를 나타낼 수 있다. 이에 본 연구에서는 역가 시험 결과꼭 편차를 촉소화 할 수 있는 시헌방법을 극힙하고자 Ok퍼 백신 주와 세로 주간 및 기타 요인에 따른 차이를 비교 분석하고 이를 표준확하여 일관겅있는 국가 및 제조사의 품질관리예 활용하고 나아가 수두 생 바이러스 백신 역가시헌 국가 표준품 거달에 활용하고자 하는데 목적이 운.다. 수두 생 바이러스 빅신의 역가 시험에 영향을 띠칠 수 꿀.는 모민으로 우선 바이러스 흡착에 사용되는 1#Z)·'diluent medium이 가장 큰 오인으로 확인되.고. 현개까기 최종적으로 if4 sucrose, 10% FBS, 0.1% sodiun) rrlonog)utamate in PBS(-), fIH 7.2가 가당 두수글 L'z).「 린luent medium 조성으로 확인되었단. 또한 '·:~l포 주별로 다소의 차이는 잇.으나 최대 바이러스 흡착시간은 90분으론:÷잰착인 되었다. 다른 negati)Fe effect를 보일 수 있는 요인으로 항생물질 및 항츤 제인 fuugiBone이 곡이러스의 역가에 영향을 미치는 것으로 확인되었다 따라서 이러한 항생물 띤 항균제를 standard solid agarose overla?·(5.tO) 시헌방법에는 사용하지 않는 것이 역가 시험의 편차를 줄일 수 있는 요인이라 판단된다. 최적의 조건하데서 Oka 수두 백신 주의 j 종의 세포 쭈에 대한 역가시험 수행 결과 각 세포 주의 감수성의 차이는 크지 않았으나, 반복시헌에 대한 표준편차는 다소 차이를 나타났다. Until now in Korea, the international and domestic vaccine maufacturers produce and sale live attenuated daricella vaccines, used to prevent chickenpox and shinglees, which are propagated in and done the potency test with various originated human diploid embryonic lung cells. Also manufacturers perform in vitro plaque assay with a little different methods to their quality control tests. Therefore, in general, there is slight or big difference among in vitro plaque assay results because of the difference in susceptibility of various originated human diploid embryonic lung cells and in assay methods. The purpose of this study is to confirm what factors occurring deviation to establish the standardized method which minimize deviation intracellularly and intercellularly. Eventually the standadized method will be used in consistent national and manufacturer's quality control and moreover intra and inter laboratory's validation assay of national standard for live varicella vaccine candidate. We confirmed VZV diluent medium was the biggest factor influencing in vitor plaque assay and the excellent composition of VZV diluent medium was final 5% sucrose, 10% FBS, 0.1% sodium monoglutamate in PBS(-), pH 7.2. We confirmed 90 minutes was maximum and optimum VZV adsorption time, but a little different among cells Also we recommended not use antibiotics and antimycotic, fungizone in standard solid agarose overlay assay in varicella potency test. Eventually 5 different originated human diploid embryonic lung cells showed little difference in susceptibility to Oka vaccine strain, but showed a lot of difference in reproducibility.

오렌지로부터 아플라톡신B₁의 정량분석에 관한 연구

장향동,백광균,구경림,옥선미,최진석,고은주,류성렬 한국공업화학회 2004 응용화학 Vol.8 No.2

Aflatoxin B₁ was known to have the strongest poisonous character among Aflatoxin compounds. To search the production of Aflatoxin B1 according to the change of temperature, relative humidity and incubation days, orange incubated with Aspergillus parasiticus KCCM 35078 was incubated at 20℃, 25℃, 30℃ at relative humidity 60%, 70%, 80% and for incubation time 3, 4, 5, 6, 7 days.. Production of Aflatoxin B₁ shows that the highest production appeared at 25℃ and its relative humidity 70%. The result that was quantitatively analyzed by using HPLC was 77.41ppm, and by using Fluoroscence detector was 87.87ppm. - Production of Aflatoxin B₁ shows that the lowest production appreared at 20℃ and its relative humidity 80%(HPLC), 60%(Fluoroscence detector). The result that was quantitatively analyzed by using HPLC was 4.47ppm, and by using Fluoroscence detector was 4.17ppm. The structure of Aflatoxin B₁ was identify with authentic sample by using instrumental analysis methods of GC/MS, ¹H-NMR, FT-IR respectively.