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정수장 슬러지 소결물을 이용한 암모늄 이온의 제거 연구
李辰河 동남보건대학 2002 論文集-東南保健大學 Vol.20 No.1
In this paper, as a method for recycling sludge from water purification plant, it was studied whether the removal of ammonium ion in waste water with sintered material which is made of sludge from water treatment plant was possible or not. In order to do this, the sludge sintered with 800℃ was put into the waste contained ammonium ion and then the changed concentrations of ammonium ion were measured by using ion-selective electrode. In the results, it was found that the removal efficiency of ammonium ion was not remarkably changed according to the sintering time and the removed amount of ammonium ion according to the input amount of the sintered material was increased at a fixed rate. But additional studies for a completely commercial use is demanded.
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이진하,조양환,조봉연,라문진,김선영,강일준,한경찬,이옥환 한국식품위생안전성학회 2016 한국식품위생안전성학회지 Vol.31 No.4
This study was assessed stability of ethanolic extract from Cirsium setidens Nakai that will be applied for development of functional foods and ingredients. We evaluated pectolinarin content, total phenol content and antioxidant activity (DPPH radical scavenging activity and FRAP assay) of ethanolic extract from C. setidens Nakai against various temperature(4, 25 and 50℃) and pH(4.0, 7.0 and 10.0). Our results show that the pectolinarin and total phenol contents in ethanolic extract from Cirsium setidens Nakai slightly reduced during the storage periods. Moreover, the DPPH radical scavenging activity at 4℃ was higher than those of other temperature. Pectolinarin content, total phenol content and DPPH radical scavenging activity of ethanolic extract from Cirsium setidens Nakai at acidic (pH 4.0) and neutral (pH 7.0) pH ranges were higher than at alkaline pH ranges. These results indicate that the optimum storage condition of C. setidens Nakai ethanol extract are temperature 4℃ and pH 4.0-7.0 ranges.
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이진하,남승희,Young-Min Kim,Nahyun Kim,Ghahyun Kim,Eun-Seong Seo,강성수,Hyen Joung Park,김도만 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.3
Appreciably elevated levels of dextranase from Arthrobacter oxydans (AODex) isolated from sugar-cane farm soil was resulted from the culture on the Luria-Bertani (LB) medium containing 1%(w/v) soluble starch,glycerol, or dextran. The responsible gene (aodex) was cloned, its nucleotide sequence was determined, and expression of the encoded protein was achieved in Escherichia coli. An open reading frame was composed of 1,863 bp putatively encoding a 68.3 kDa protein. Recombinant A. oxydans dextranase (rAODex) was purified about 16 fold by nickel-nitrilotriacetic acid affinity column chromatography;Km value for dextran T2000 was 0.85 mg/mL (w/v). AODex treatment of stale sugar cane juice resulted in a yield of square and light-colored sugar crystals.
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DNA Breaking Action of Guanine Analogs Modified by Triose Reductone
이진하,Lee, Jin-Ha 생화학분자생물학회 1983 한국생화학회지 Vol.16 No.3
Triose reductone으로 수식된 guanine 유도체의 $\lambda$ 및 calf thymus DNA에 대한 절단작용을 agarose electrophoresis로 검토하였다. Tricylcic type인 $N^2$-(2-hydroxypropenylidene) guanin과 open type인 $N^2$-(3-oxo-2-hydroxypropenyl) guanine은 $CuSO_4$ 존재하에서 DNA 절단작용을 가지고 있으며 후자가 더 강한 활성을 가지고 있다. 또한, 그 guanine 유도체들의 DNA에 대한 절단작용은 triose reductone 보다 약한 활성을 나타냈다. Double strands breakage of guanine analogs modified by triose reductone on $\lambda$ and calf thymus DNA was investigated by using agarose slab gel electrophoresis, $N^2$-(2-hydroxypropenylidene) guanine and $N^2$-(3-oxo-2-hydroxypropenyl) guanine, possessed, the DNA breaking activity in the presence of $CuSO_4$, and the latter was more active than the tricyclic type analog. The DNA breakability of the analogs was weaker than that of triose reductone.
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이진하,김도만,Young-Hwan Moon,정연진,Kyung-Joo Seong,임회순,김선헌,김원재,정지연 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.5
(-)-Epigallocatechin-3-gallate (EGCG) has been reported to inhibit cellular proliferation and induce apoptosis in a range of cancer cells. This study examined the cytotoxicity of EGCG glucoside on human laryngeal epidermoid carcinoma Hep2 cells. The EGCG glucoside treatment decreased the cell viability in Hep2 cells. Furthermore, EGCG glucoside caused apoptotic morphological changes with chromatin condensation and nuclear fragmentation, as observed by a terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and activated caspase-3 immunohistochemisty. However, the EGCG glucoside did not induce the production of reactive oxygen species (ROS), suggesting that oxidative stress is not involved in the apoptotic response. EGCG glucoside induces apoptosis in Hep2 cells through not the generation of ROS, but the activation of caspase-3.
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Effect of pH and Ionic Strengths on the DNA-Breaking Action of Some Guanine Analogs
이진하,최종미,김금수,Lee, Jin-Ha,Choi, Jong-Myee,Kim, Geum-Soo 생화학분자생물학회 1989 한국생화학회지 Vol.22 No.2
구아닌 관련 화합물과 2,3-dihydroxy-2-propenal과의 반응에서 분리된 수종의 구아닌 유사체들의 DNA에 대한 작용을 조사하였다. 각종 pH를 가진 다양한 완충용액 중에서 DNA와 이들 유사체와 반응을 행하여 이들 화합물이 pH나 이온의 강도에 따라 DNA 절단 기능이 다양하게 영향을 받는 것이 확인되었다. The physiological function of some guanine analogs separated in the reaction of guanine related compounds with 2,3-dihydroxy-2-propenal on double strand DNA was investigated. The reaction of the analogs with calf thymus DNA was carried out in various ranges of pH and in each buffer solution with different ionic strengths. The DNA-breaking action of the compounds was affected by pH, ionic strengths and species of ions.
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