증폭된 HIV - 1 DNA의 Digoxigenin표식 Probe에 의한 검출

허숙진,강춘,Yutaka Takebe 대한바이러스학회 1990 Journal of Bacteriology and Virology Vol.20 No.2

By means of a selective DNA amplification technigue called polymerase chain reaction, a region of the LTR gene, gag gene, encompassing the core protein p 24 and env gene, encompassing the envelope protein gp 41 was amplified. Each synthetic oligonucleotide primer pairs was used to amplify DNA from conserved nucleotide sequence particularly. Because primer pairs from multiple regions of the HIV-1 genome can be used to achieve maximum sensitivity of provirus detection. Amplified HIV-1 DNA from AIDS patients, seropositive, and seronegative persons was detected. This method of DNA amplification made it possible to obtain results within 2 days, whereas virus isolation takes up to 3 to 4 weeks. The method may therefore be used to complement or replace virus isolation as a routine means of determining HIV-1 infection or to provide direct identification of HIV-1 infected seronegative persons.

국내 유통 소금 중의 포타슘시아니이드와 페로시아나이드염의 분석에 관한 연구

허숙진,소유섭,최장덕,이화정,최선희,성준현,임무혁,김광수,최용훈,오해성,최정희,최정실,허옥순,이진하,신동우,신현수,문병우 식품의약품안전청 2001 식품의약품안전청 연보 Vol.5 No.-

본 연구는 국내 유통 소금중의 안전성을 규명하기 위하여 천일염 54건, 재제염 보건, 가공염 33건 등 총 143건에 대하여 숲행하였다. HPLC, ICP, Mercury Analyzer 등을 이용하여 KCN, 페로시아나이드 염, 납 등 6개 성분의 항량을 측정하였으며 그 결과는 다음과 같다. [rdn-max(mean) ; m곱3 ; f(CN : ND-ND(ND), 페로시아나이드염 : ND-ND(ND), Pb . ND-1.30 (0.331), Cd :ND-0.23(0.070), Hg ND-0.35(0,035), As :ND-1.52fo.062). 또한, 신속하고도 효율적이 면서 기존의 환경부 시안분석법의 단점을 보완할 수 있는 KCN과 페로시아나이드염의 분석 조건을 계시하였으며, 천일염, 재제염, 가공염 뿐만아니라 소금이 첨가된 식품까지도 적용하기 위한 기초 자료로 활용이 가능할 것으로 사료된다. This study was conducted to confirm the safety in a total of 143 samples of salts. The contents of potassium cyanide(KCN), potassium ferrocyanide(K_(4)Fe(CN)_(6)), lead(Pb), cadmium(Cd), mercury(Hg), and arsenic(As) were determined using a high performance liquid chromatograph(HPLC), inductively coupled plasma spectrometer(ICP), and mercury analyzer. The values of cyanide complexes and metal elements in salts were as follow [min-max(mean);mg/kg] ; KCN : ND-ND(ND), Ferrocyanide salt : ND-ND(ND), Pb : ND-1.30(0.331), Cd: ND-0.23(0.070), Hg : ND-0.35(0.035), As : ND-1.52(0.062). In this study, we established efficient

중합효소연쇄반응을 이용한 후천성면역결핍증 바이러스의 진단

허숙진 대한감염학회 1991 감염 Vol.23 No.4

AICD(후천성면역결핍증:acquired immunodefiency syndrome)는 사람면역부전바이러스(HIV)의 만성지속성감염에 의하여 일어나는 면역결핍상태를 중심으로 전신에 퍼지는 기회감염증, 악성종양, 정신신경계장해를 수반하며, 일단 감염이 된 후에는 효과적인 치료제도 거의 없을 뿐 아니라 결국에는 죽음에 이르게 하는 질환으로 그의 발증 예방, 치료의 어려움과 높은 발증률, 사망률등으로 세계 공통의 문제로서 지대한 관심사가 되고 있다. 따라서 HIV에 감염되지 않도록 예방을 최우선적으로 해야 하며 또한 일단 감염된 사람으로부터 더 이상의 전파를 방지하기 위해서는 HIV의 감염여부의 조기진단이 필수적이라고 하겠다. 현재 HIV에 감염된 사람을 검사하는 일상진단법으로 viral epitope에 대한 항체검사방법이 있으나 1) HIV에 감염되어 있으나 그 혈액 내에 항체가 형성되지 않는 window period가 있는 점. 2) 태반을 경유한 모체로부터 이행한 IgG항체가 생후 15개월까지 소아의 혈중에 머무르고 있으므로 HIV항체를, 감염의 유무를 나타내는 신뢰성 있는 지표로서 사용할 수 없는 점. 3) 신생아, 유아로부터 채취할 수 있는 혈액량은 대부분의 경우 극히 제한되어 있다는 점. 등으로 완전한 검사방법이라고는 말할 수 없다.

HIV진단시약에 대한 HIV감염자의 항체 반응성에 대한 연구(Ⅱ)

허숙진,장영식,김태숙,최민호,김성순,기미경,전원경,박미애,?권석,김태규,신영오 국립보건연구원 1994 국립보건원보 Vol.31 No.1

국내 Human Immunodeficiency virus(HIV) 감염의 역학 및 면역학적 특성

신영오,장영식,허숙진,기미경,김성순,최병선,최민호,전원경,채권석,이영종 국립보건연구원 1994 국립보건원보 Vol.31 No.1

HIV 감염자에서 CD4+T 림프구수, β₂-Microglobulin 및 HIV p24 항원

조영걸,신영오,최병선,김태숙,허숙진,기미경,김성순,강춘,조양자,김영봉,조군제 대한감염학회 1993 감염 Vol.25 No.2

We have measured CD4+T lymposite , β²-MG) and p24 antigen level in 188 HIV patients Estimated duration presumptive HIV inpection last tested CD4+T lymphocyte counting was 55.2±23 months in group with Zidovudine (Acidothymidine, AZT) treatment(Tx) and 38.4±20 months in group without AZT. There was no CD4+T lymphocyte of less than 400/mm³in 56 normal persons but 46% (86) were CD4+T lymphocyte of less than 400/mm³in 188 HIV patients. Annual decrase of CD4+T lymphocyte counts was 70/mm³in group without AZT tx and 38/mm³in group with AZT tx months after tx start. In the distribution of serum β₂-MG level in 182 HIV patients and 48 normal control, 90% (43) of control were below 2.0 mg/L and 76%(139) of HIV patients were over 2.0mg/L (p=0). β₂-MG level was negatively correlated with CD4+T lymphocyte (r=-0.394,<0.001). HIV p24 core antigen was detected in 26 (14.9%) out of 174 HIV/AIDS patients. Of the 26 patients, 21(81%) were CD4+T lymphocyte of less than 400/mm³and 46% (12) of less than 200/mm³. In was confirmed that 3 tests mentioned above are useful markers for prognosis of HIV infected persons.

Clq-coated ELISA법을 이용한 정맥용 면역글로불린제제의 항보체성 측정

강혜나(Hye Na Kang),김순남(Soon Nam Kim),신광훈(Kwang Hoon Shin),허숙진(Sook Jin Hur) 大韓藥學會 2001 약학회지 Vol.45 No.6

The quality of an intravenous immunoglobulin preparation (IVIG) is reflected by the degree of nonspecific activation of complements, the so-called anticomplementary activity (ACA). ACA of aggregates in IVIG was investigated using method by the European Pharmacopoeia and Clq-coated microtiter enzyme-linked immunosorbent assay (ELISA). Both the EP method and the ELISA method showed a dose response curve with the amount of complements bound increasing with the percentage content of aggiegates in immunoglobulin standard. The correlation between the two tests was good (r=0.96, r=0.99). However the correlation was not found when the ACA (EP method) of IVIG product was compared with its aggregate percentage. These results emphasize that the method of aggregate formation affects ACA and that estimation of the percentage distribution of aggregates by HPLC may not reflect ACA. In analysing IVIG product for Clq binding activity test with the ELISA, the result by using Protein A-HRP correlated with aggregate percentage (r=0.84). But the correlation decreased (r=0.48) when the result used Protein A-AP(having poorer sensitivity than HRP) was compared with aggregate percentage. As a result, some variation between the two methods, due to differences in assay principles, is to be expected. However ELISA technique has the advantage in that it is easier to perform, more precise and less subject to reagent variability and is the more suitable screening method than HPLC analysis.

합성기질 및 응고시간을 이용한 혈액응고 제8인자 역가측정법

강혜나(Hye Na Kang),김순남(Soon Nam Kim),허숙진(Sook Jin Hur),홍성화(Seung Hwa Hong) 대한약학회 2001 약학회지 Vol.45 No.5

The clotting assay was replaced by the chromogenic substrate assay which is recommended by the European Pharmacopoeia (EP) and the Scientific and Standardization Committee of the International Society on Thrombosis and Haemostasis based on the reliability convenience and simplicity of the chromogenic assay: A correlation study was carried out with a one-stage factor VIII : C clotting assay and the perfonnance of the chromogenic assay was evaluated using two test kits that fulfilled the requirements of EP for factor VIII concentrates test. Although chromogenic assay has partly differences in measurement principle and standardization, this assay has a high correlation with clotting assay in various types of factor VIII concentrates and factor Vlll standard. We conclude that the chromogenic assay for factor VIII : C con- centrates correlates well with the clotting assay and shows good analytical performance.

ASE를 이용한 식품원료용 생약재의 잔류농약 분석방법 연구 및 모니터링

이동호,김형수,송병권,신영민,손영욱,정지윤,안경아,최자인,허숙진,이길응,정정순,임무혁,김우성,홍무기 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-

생약재의 잔류농약을 분석하기위한 새로운 겋사방법의 개발과 업무의 효율화를 재고하기 위한 방안의 일환으로 가속용매추출장·치(Accelerated solvent extractor,ASE)를 이용한 분석방법 확링을 시도하였다. AS도의 운용조건은 acetone을 추출용매로 하여 1,000psi, 4et에서 운용하는 것이 최적의 조건이었고 이들 추출액의 정제는 prewashing 용매로 hexane을 Smf 사용하여 Florisil 카트리지를 활성화 한 후 10m4의 20% acetone 포화 hexane으로 용출하는 것이 정제효과면에서 가장 좋았다. 생약재에 있어서 대부분의 시료에서 70%이상의 회수율을 나타내었으며 최저 검출한계치는 0,O04~o.016r9/me로 매우 낮았다. 또한 위와 같은 분석방법을 이용하여 165종의 생약재에서 모니터링을 한 결과 7건에서 농약이 검출되었으나 그양은 0.118-0.045ppm으로 극미량 이었다. This study was carried out to improve the analytical method and monitoring of pesticides in natural medicines using Accelerated Solvent Extractor(ASE). Optimiui~ extractconotions for ASE were at 40'Cr 1000psi with acetone. Extracts were puffied through SPEflorisil cartridge with 10ee of 20% acetone saturated hexaRe. Recoveries from samples weregreater than 70% most of the natural medicines. The limit of detections(LODs) in all matrixwere 0.O04~o.016 #g/mf.The residual pesticides ·rrere detected in 7 samples of 165 natural medicines. However, thedetected pesticides were very small amount ranged from 0.045 to 0.118 #g/ma.

패류중 중금속함량에 관한 연구

신현수,허옥순,이진하,김세은,주인선,신동우,강석종,박만종,김지연,김근희,강숙경,정래석,허숙진,소유섭,최장덕,문병우,김진수 식품의약품안전청 2001 식품의약품안전청 연보 Vol.5 No.-

본 연구는 패류중 중금속함량 측정을 위한 분석효율이 높고 간편한 분해법을 찾아내어 그 방법에 의끓 유종중인 패류중 중금속함량을 파악하고자 수행하였다. 시료를 분해시키는 Microwave와 습식, 건식회법을 비교한 결과 변형습식회화르가 가장 적합하였다. 변형습식회차딘는 려린0,3+C104+H2SOf의 혼찰산을 쪼 · j 1 (v/v)의 비율로 혼합하여 사용하였 을 때 회수을 Pb린틴. Cd 97.8, Cu 94.2, Zn 92.8%를 나타내었근 분해시간은 2~2.5시간 소요되었다. 유통중인 패류중 중금속함량 」최소~최대(평균), mgAgl 은 Pb 0.028~o.528(0.153), Cd 0.OO7~1,832(0.587), Cu 0.360~37.375(4.6691, Zn 6.317~233.2iO(38.881), Hg 0.001 ~O.151(0.012)mg/ltg으로 나타났다. 또한 유통중인 5종의 패류중 중금속평균항량이 생산지 하재지역에서 구입한 패류에 비해 모든 중금속언써 약간 높은 함량을 보였으나 오염된 수준이 아닌 자연함유량수준이었다. This study was conducted to improve the digestion method for the determination of heavy metals in shellfishes and to estimate the contents of heavy metal in commercial shellfishes by their digestion method. This digestion method was compared microwave with wet and dry ash digestion for shellfish samples, obtained results showed that the modified wet ash Ⅱ digestion was convenient and easy to use. For the modified wet ash Ⅱ digestion, The mixed acid of HNO₃+HClO₄+H₂SO₄(25:5:1, v/v) was used. Under this condition, Recoveries were Pb 94.3, Cd 97.8, Cu 94.2 and Zn 92.8% and digestion time was ∼2.5 hours. The contents of heavy metal [minimum∼maximum (mean), mg/kg in commercial shellfishes were as follows ; Pb:0.028∼0.528(0.153), Cd:0.007∼1.832(0.587), Cu:0.360∼37.375(4.669), Zn:6.317∼233.250(38.881), Hg:0.001∼0.151(0.013). The contents of heavy metal in commercial shellfishes of five species were slightly greater than those of shellfishes purchased at Hajae. There was no great difference between the contents of heavy metal of Hajae and those of shellfishes purchased at Whole market.