2-O-α-D-Glucopyranosyl L-Ascorbic acid 생산을 위한 Cyclodextrin glucanotransferase의 고정화

성경혜,김성구,장경립,전홍기 부산대학교 김치연구소 2003 김치의 과학과 기술 Vol.9 No.-

AA를 AA-2G로 전환하는 CGTase를 고정화하여 AA-2G 대량 생산의 가능성을 고찰한 결과, CNBr-sepharose 4B가 가장 효과적인 담체로 판명되었고, CGTase의 고정화 최적 조건과 고정화 CGTase에 의한 AA-2G 생산의 최적조건 및 재사용성을 검토하였다. 최적 고정화 조건은 효소량 24,000 units/g resin으로 9시간 반응하여 약 18,000 units/g resin의 최고 고정화율을 얻을 수 있으며, pH 5.0(50 mM sodium citrate buffer)용액에 12% AA-Na와 8% soluble starch를 기질로 하여 800 units/ml의 고정화 CGTase를 첨가한 후 37℃에서 100 rpm으로 교반하면서 25시간 반응하여 약 18 mM의 AA-2G 최고량을 얻을 수 있었다. 또한 0.015 mM의 CaCl₂를 첨가하여 고정화 CGTase의 재사용성을 관찰한 결과, 5회까지 50% 이상의 AA-2G 생산율로서 그 가능성을 입증할 수 있었다. 그리고 효소의 재사용성이란 측면에서, 본 고정화의 다른 한 방법인 ultrafiltration(한외 여과)에 의해서는 Millipore사의 YM10 membrane을 이용하여 먼저 단백질량과 효소 활성 변화를 측정하여 그 가능성을 확보할 수 있었으며, 기질 20 ml을 사용하여 AA-2G를 생성시킨 후, 한외여과에 의해 효소만을 회수하여 연속 반응해 본 결과 8회까지 50% 의 생성률을 유지하였다. 따라서 한외 여과는 CNBr-sepharose 4B와 함께 효율적인 고정화의 한 방법으로 판명되었으며, 앞으로 이들 고정화 효소를 이용한 연속 반응 시스템의 구축이 뒤따라야 할 것이다. Cyclodextrin glucanotransferase (CGTase) from Paenibacillus sp. JB-13 was immobilized on various carriers by several immobilization methods such as ionic binding, covalent linkage and ultrafiltration to improve the process performance. The ultrafiltration and covalent linkage with CNBr-activated sepharose 4B were found as the best method for immobilization of CGTase. The ability of was as follows: contact time 6 hrs at 37℃, pH6.0, 100rpm and enzyme loading 24,000 units/g resin. The optimum conditions for production of 2-O-α-D-Glucopyranosyl L-Ascorbic acid by immobilized CGTase turned out to be: pH 5.0, temperature 37℃. 20% substrate solution containing 8%(w/v) of soluble starch and 12%(w/v) of L-ascorbic acid sodium salt, 100 rpm, for 25 hrs and with 800 units of immobilized CGTase/ml substrate solution. More over the CGTase activity could be stably maintained for 8 times of repetitive reactions after removing products by ultrafiltration through YM 10 membrane.

저체중 또는 과체중 청소년에서 캠프 프로그램을 이용한 영양 교육효과에 관한 연구

성미경,승정자,류화춘,박재년,박동연,최미경,조경옥,최선혜,이윤신,김유경,이은주 대한지역사회영양학회 2003 대한지역사회영양학회지 Vol.8 No.4

This study was performed to evaluate the effectiveness of a nutrition education program developed for nutritionally imbalanced adolescents. A summer nutrition camp was held for 23 overweight and 16 underweight subjects. Its effectiveness with regard to was evaluated at the end of the camp and 6 months later. Nutrition knowledge, nutritional attitude, food behavior, nutrient intake, exercise habit and ideal body figures desired by the subjects. The results showed significantly higher nutrition knowledge scores at the end of the camp as compared to those obtained prior to the camp program, and these scores were maintained for at least six months. Nutrition attitude scores also improved after the education program, and these improved scores also lasted for 6 months. However, the food behavior scores measured 6 months after the education program were not significantly different from those obtained prior to the camp. Also, the exercise habit, the ideal body figures and the body figures desired by the subjects remained unchanged. When nutrient intakes of subjects were assessed before the program and 6 months later, the mean daily vitamin C intake was significantly increased after the education program. Also, the intake of iron from plant food sources increased in the overweight subjects, while less iron from animal source were consumed by the underweight subjects. Both groups tended to consume more vegetables and fruits 6 months after the education program which may have contribute to the higher vitamin C and plant-based iron intakes. These results indicate that a 4-day nutrition education camp program sustained changes in nutrition knowledge and nutrition attitude for 6 months. The increased intake of vegetables and fruits was also achieved through this education program. However, changes in dietary behavior in adolescents may require repeated education.

Extension of Lance Life by Change of Height of Lances in the Smelting Furnace of Mitsubishi Process

Sung‑Mi Lim,Soo‑Sang Park,Kyung‑Woo Yi 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.10

A Mitsubishi process, which pertains to continuous Cu production, involves a smelting furnace, cleaning furnace, and convertingfurnace connected in series. One of the main issues pertaining to the smelting furnace is the frequent interruption ofoperations required to allow the inspection and replacement of lances, because lances are frequently fractured. The presentstudy was aimed at modifying the operating conditions of the smelting furnace to suppress lance fractures. A numericalmodel was developed to simulate the transport phenomena in the furnace, including multi-phase behaviors. The simulationresults showed that the lances were exposed to a severely erosive atmosphere with high temperatures. Further calculationindicated that raising the positions of the lances could lower the temperature of the lances, and reducing the occurrence ofsplashed melt, which contains erosive sulfides. The smelting furnace was operated under the conditions of the raised lanceheight and attentively monitored for several months. It was confirmed that by implementing such a change of the lanceheights, the occurrence of lance failures has been considerably reduced without notably affecting the reaction ability of thesmelting furnace.

생약제제인 이공산(異功散)의 Cisplatin 유도 신장독성 보호 및 항산화 효과

Lee, Kyung-Tae,Ahn, Kyoo-Seok,Chang, Sung-Goo,Oh, Soo-Myung,Jung, Jee-Chang 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Kyung-Tae Lee, Kyoo-Seok Ahn¹, Sung-Goo Chang², Soo-Myung Oh²and Jee-Chang Jung²¹College of Pharmacy, ²Oriental Medicine and ³College of Medicine, and East-West Medical Reserch Institute, Kyung Hee University, Seoul, Korea. Preventive and Antioxidative Effects of Crude Drug Preparation(E-kong-san) on Cisplatin Induced Nephrotoxicity. Proceedings of International Symposium on East-West Medicine, Seoul. 201-211, 1999. -Nephroprotective effects of a crude drug-prearation (E-kong-san) were determined from cisplatin on rabbit kidney proximal tubule and human renal cortical cells by MTT assays and sustained glucose consumption on ciplatin-induced human renal cortical tissue. Levels of creatinine and blood ure nitrogen(BUN) in serum after administration of cisplatin(0.75mg/kg,i.p.) to E-kong-san(0.75g/kg/day,p.o.)-pretreated rats were markedly lower compared to those of cisplatin-treated rats. Moreover, the administration of E-kong-san significantly inhibited the loss of body weight of cisplatin injected rts. These findings suggest that E-kong-san on the cisplatin induced nephrotoxicity led us to investigate whether the effect of this water extract was a result of anti-oxidation. E-kong-san showed strong free radical scavenger activities on 1,1-dipheny1-2picrylhydrazil(DPPH) radical and xanthine/xanthine oxidase(XOD) generated superoxide anion radical(O2-). We further studied the effects of E-kong-san on lipid peroxidation in rat liver microsomes induced by enzymatic and nonenzymatic methods. E-kong-san exhibited significant inhibition on both ascorbic acid/Fe2+and ADP/NADPH/Fe3+ induced lipid peroxidation in rat liver microsomes. Based on these results, we suggest that-E-kong-san attenuate the cisplatin induced cytotoxicity and its mechanism can be eplained by antioxidant.

항암제 AG60이 흰생쥐 위점막 상피세포의 DNA합성에 미치는 영향 : 자기방사법적연구

박경호,안의태,고정식,하상선,한영복,정영신,홍은경,유보림,김상건,이경영,강종구 대한해부학회 1999 Anatomy & Cell Biology Vol.32 No.1

The effect of neuropeptides on secretion of Interleukin-8(IL-8)

Kim, Kyung-Jun,Park, Sang-Hyuk,Choi, Kyoung-Kyu,Park, Sang-Jin 大韓齒科保存學會 2006 Restorative Dentistry & Endodontics Vol.31 No.3

본 연구는 치수조직, 치은, 치주인대로부터 배양된 조직을 SP (Substance P)로 4시간, SP, CGRP (Calcitonin Gene-related Peptide), Tumor necrosis factor-α (TNF-α)로 8시간 자극 후 RNase Protection Assay를 시행하고, IL-8의 분비량을 측정해 다음 결과를 얻었다. 1. IL-8 mRNA는 모든세포에서 발현됐다. 2. IL-8 mRNA 발현은 SP (10^(-5)M)와 SP (10^(-8)M)로 4시간 자극 시 증가되지 않았다. 3. IL-8 mRNA 발현은 SP (10^(-4)M)와 CGRP (10^(-6)M)로 8시간 자극 시 증가되지 않았다. 4. TNF-α (2 ng/㎖) 자극 시, IL-8 mRNA 발현이 증가됐다. 5. 치은 세포를 CGRP (10^(-6)M)로 8시간 자극 시, IL-8 분비량이 증가했다 (p < 0.05). 6. 치주인대 세포를 SP (10^(-4)M)로 8시간 자극 시 IL-8 분비량이 증가했다 (p < 0.05). We investigated the secretion of Interleukin-8 (IL-8) from ginviva and periodontal ligament stimulated with Substance P (SP) and Calcitonin Gene-related Peptide (CGRP). Gingiva (GF), periodontal ligament (PDLF) and pulp (PF) tissues were collected from extracted instact 3rd molars. Cultured cells were stimulated with different concentrations of SP for 4 hrs, and stimulated with SP, CGRP and Tumor Necrosis Factor-α (TNF-α) for 8 hrs. Then RNase Protection Assay was carried out. ELISA was performed using supernatants of stimulated cells for quantitative analysis of IL-8. Results were assessed using supernatants of stimulated cells for quantitative analysis of IL-8. Results were assessed using student t-test with significance of P < 0.05. According to this study, the results were as follows: 1. IL-8 mRNA was detected in all type of cells studied (PF, GF and PDLF). 2. IL-8 mRNA expression was not increased after stimulating 4 hrs with SP (10^(-5)M) and SP (10^(-8)M) compared with Mock stimulation in all type of cells studied. 3. IL-8 mRNA expression was not increased after stimulating 8 hrs with SP (10^(-4)M) and CGRP (10^(-6)M) compared with Mock stimulation in all type of cells studied. 4. TNF-α (2 ng/㎖) increased the expression of IL-8 mRNA in all kind of cells studied. 5. The secretion of IL-8 from GF was increased 8 hrs after the stimulation with CGRP (10^(-6)M) (p < 0.05). 6. The secretion of IL-8 from PDLF was increased 8 hrs after the stimulation with SP (10^(-4)M) (p < 0.05). Calcitonin Gene-related Peptide (CGRP) increased Interleukin-8 (IL-8) which plays an important role in chemotaxis of neutrophil in Calcitonin Gene-related Peptide (CGRP) gingival tissue, whereas Substance P increased the secretion of IL-8 from periodontal ligament.

『대한신문(大韓新聞)』과 이인직

이상경(Lee, Sang-Kyung) 한국어문학회 2014 語文學 Vol.0 No.126

The present study seeks to elucidate the Daehan Sinmun (Korean Newspaper)’s contents through a source book (hereafter “excerpt collection”) transcribing editorials and some articles published in the daily from July 18, 1907 (first issue) to December 28, 1909 and to analyze the logic of journalists as represented by In-jik Yi (1862-1916). First, upon comparison between articles in the Daehan Maeil Sinbo (Korea Daily News) critical of the Daehan Sinmun and the excerpt collection, it was possible to confirm that the latter served as the original source for the collection of excerpts. Second, “A Song of the Righteous Armies Longing for Home” (Uibyeong Sahyang-ga) in the excerpt collection, a work that had sought to persuade members of the anti-Japanese righteous armies (uibyeong) to surrender, was similar in its logical structure to the speech made by Ong-nam Choe in the latter half of the novel The Silver World (Eunsegye) encouraging members of the righteous armies to capitulate.

문화권 개발을 통한 관광ㆍ문화산업 육성방안에 관한 연구 : 경북북부 유교문화권을 중심으로

황경성 문화관광연구학회 2000 문화관광연구 Vol.2 No.4

In the 21st century, Culture will be a dominant factor in every field of our life like the high quality, a way for settlement of our racial identity, a reunification. One thing that is important in culture is that culture can show strong effects of the regional community. The regional culture that is connected with territory is the expression of regional spirit and the way of the regional life. Therefore, development of regional community in real meaning is to make prejudice and wrong recognition correct. First of all, we should get rid of the fixed thinking that regard culture as only a thing for consumption and economy. The right culture is one of rights for human beings the right to be free. We need to consider the satisfaction of cultural demand, realization of cultural welfare, supply of cultural education first. Also in the process of development of certain culture band, it will be an essential thing to insure efficiency, equity, and harmony of many other regional places which are under the influence of certain cultures in a wide area. In this research, we apply this recognition to the development of confucianism in north side of kyung Sang province of Cho-sun dynasty. We think about the development of culture band and the way of the upbringing of tourism. And here are works for accomplishment : First development of history and culture band. Secondly, organization of networks for regions and subjects. Thirdly, the development of resort connected with historical and cultural band. Fourthly, establishment of a confucian information center. Fifthly, event and special business plan for region. In addition we are supposed to suggest something . First designation "historical and cultural special area" of the north side of Kyung Sang province, Second cooperation of administrative districts, between governors and citizens, Third education of citizens and the development of human power. We have to confess that this research is still in the first step with many control things. It is true that wide and deep study can help the main work from this research.

운하-연안 연계용 바아지의 구조 설계 및 해석

김경수(Kyung-Su Kim),손충렬(Choong-Yul Son),김성준(Sung-Joon Kim) 한국해양공학회 1999 韓國海洋工學會誌 Vol.13 No.2

Two types of barge, container and tanker vessels, are designed with specific dimensions for canal-coastal shipping in Kyung-In canal and Yellow Sea region. The principal dimensions of barges are determined by considering the environment of Kyung-In canal and Yellow Sea. The selection of structure type and strength confirmation is conducted by the technical rules of Korean Register of Shipping.<br/> For the structural analysis of designed barges, the semi-direct structural analysis is carried out with using MSC NASTRAN software. Applying identical dimensions to both vessels, the standardization of structural design and analysis procedure are introduced.

사상체질과 신체형태지수와의 연관성

박경아,김희진,허경석,안수경,이성호,이종은,이원택 대한췌담도연구회 2000 대한췌담도연구회지 Vol.5 No.4