서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1997)

김재홍,문득곤,김정수,김용준,임동진,박상훈,김희성,이민수,송기훈,김갑형,김형석,성소영,이인섭,김석우,황지환,조창근,김경문,부태성 대한화학요법학회 2000 대한화학요법학회지 Vol.18 No.3

Background : In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. Objective & Methods : For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results : In 1997. 99 strains of N. gonorrhoeae were isolated, among which 45(45.5%) were PPNG. Conclusion : The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 45.5% in 1997.

독성물질의 세포사 기전 및 세포사 유발물질의 검색법 개발에 관한 연구(Ⅰ) : 독성물질로 인한 파킨슨병 모델에서의 세포사 기전 연구 Study on the cell-death mechanisms of toxin-induced parkinsonism

강태석,김종민,서경원,김영옥,김준규,오재호,이윤동,김규봉,오정자,송연정,임종준,전범석,문전옥,최광식 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-

MPTP 독성물질이 도파민성 신경세포에 선택적으로 작용하여 산화성 손상에 의한 신경세포사를 일으키는 것을 이용하여 파킨슨병의 동물모델을 만들고, 이를 통해서 아폼토시스를 비롯한 포사의 기전에 대한 연구 및 너코틴의 신경세포 보호효과 여부를 판정하는 실험을 병행하고자 하였다. 파킨슨꾐의 동물모델을 MPTf 독성 물질을 이용하여 확립하였으며, MPTP(30mgag, i.p.)를 투여한 후 1, 2,3, 4, 5일째 흑질 조직을 채춰하여 tarm로 박걸하여 tyrosine hydroxylase 면역조직화학염색을 수행하여 cell countif우한 결과, control은 57.635ce11s, 1일째 친.OfDells,2일째 57.9±6cells,3일릴 없.3±죠ells, 4일째 49.0츠3cells, 5일째 39.4±Scells료 4, 3일째 뚜렷한 신경세포 수의 감소를 보였다. 신경세포사 기전 규명을 위한 아폼토시스 분걱에서는 벼PTP 투여 후 1, 2, 3, 4, 5일째 조직을 채취하여 Hoechst staining, TUNEL staining을 수곡하였는데 양성 반응을 보인 신경세포는 관찰되지 않아. 아폼토시스로 인한 세포사가 관찰되지 않았다. bIPTP 파킨슨병 동물모델에서 nicotine 보호효과 탐색에 관한 실험은 nicat푸e 0.2mgAg을 5일 퐁안 투여 후 리『fP(30mgag)를 CS7Bt/6 마은스에 복강 내주사로 nicotine과 병용 투여한 후 1, 2, 3, 4, 5일째 뇌를 적출하땄다. 신경세포사가 뚜렷이 관찰되기 시작하는 4, 5일째의 신경세포 수의 감소 정도를 20. 30% 정도 약화시키는 경향을 보였으나, nicotine 보호효과에 대한 추가 실헝이 현재 수행 중에 있다. The cause of Parkinson's disease (PD) is largely unknown. However, free radical toxicit? may plaf a role ip. the degeneration of substantia nigra, which is the Hajorfocus of pathological damages in PD. Recently, a neuroprotective effect of nicotine in PD has been suggested. Therefore, the mechanism of neurodegenerafion and protective potential o( nicotine in PD were investigated in the experimental modeB of Pll using a neurotoxin, C57BL/6mice were administered with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP, 30 mg/kg,j.p.). The degree of neurodegenerafion was determined by immunohistochemical stainiHB oftyrosine hydroxylase (TH). TH-positive cells on nigral sections were found 56.0 ±4, 57.9 ±6,52.315ce11s, 49.0±3cells, and 39,4±Scells at days 1, 2, 3, 4, 5, respectively (controls : 57.6±Scells). Hoechst and TUNEL staining showed no evidence of apoptosis. The exandnation on themice co-adrunistered with nicotine(0.2mgAg) and MPTP(30mgag) revealed a tendency ofnicotine protective effects. At days 4 and 5, the degree of TH-positive cells was decreased by20-30%, In corclusiffn, the role of apoptosis was not evidenced in this MPTP modeB of PB.The possible proteccon by nicotine should be elucidated with further studies.

열공형과 비열공형 피질하 혈관성 치매에서 위험인자의 차이에 관한 비교 연구

배희준,정지향,유경호,나덕렬,김상윤,최경규,양동원,손의주,이상도,김재우,박경원,김응규,이재홍,박미영,한일우,함동석,최문성,하충건,최성혜,이애영,이병철,한설희 대한치매학회 2003 Dementia and Neurocognitive Disorders Vol.2 No.2

Backgrounds and Objectives: Vascular dementia is a group of dementing disoders arising from various stroke syndrome. Among these. subcortical ischemic vascular dementia (SIVD) is regarded as a relatively distinct clinical entity. However, MRI patterns of SIVD are not homogenous. In some patients, lacunes are dominant, and in others, subcortical white matter changes are. This study was designed to compare risk factor profiles between SIVD with and without multiple lacunes. Methods: We divided 47 subjects (22 males, mean age. 68 years) recruited from VADAPET (Multicenter Trial For Evaluation Of The Changes In the PET Images Of Subcortical Vascular Dementia Patient) study into two groups one with more than 5 lacunes in deep gray matter (lacune group) and the other with 5 or less(non-lacune group) Clinical characteristics and laboratory findings of two groups were compared. Results: Nineteen of 47 patients (40%) belonged to the lacune group. The lacune and non-lacune groups d d not differ in the following variables: age, hypertension, diabetes mellitus, hyperlipidemia heart disease, history of stroke or TIA, history of trauma or major surgery, family history of hypertension stroke, or dementia, age at diagnosis of dementia, body mass index, white blood cell count, ESR, CRP, fibrinogen, hemoglobin A1C, total cholesterol. LDL cholesterol creatinine, proteinuria, glucosuria, and microhematuria. However, male sex, smoking alcohol. hemoglobin, and HDL cholesterol were possibly associated more with lacune group SIVD than with non-lacune group (p<0 1) Multivariate analyses revealed that smoking, hemoglobin, and HDL cholesterol were independent predictors of SIVD with multiple lacunes Conclusion: Our study suggests that SIVD with multiple lacunes may be significantly different in smoking habits hemoglobin, and HDL cholesterol from SIVD without multiple lacunes.

성장에 따른 백서 절치유두의 신경 분포 변화

김영진,남순현,김현정,이재문,구대학 大韓小兒齒科學會 1996 大韓小兒齒科學會誌 Vol.23 No.1

출생후 성장에 따른 절치 유두의 신경분포 및 변화를 관찰하기 위하여 생후 1, 5, 10, 15, 20, 25, 35일 된 백서와 완전히 성장한 백서를 대상으로 calcitonin gene related peptide (CGRP)를 이용한 면역조직 화학적 방법으로 관찰하여 다음과 같은 결과를 얻었다. 생후 1일군에서는 염주알 모양의 굵은 신경섬유가 성긴 결합조직에 나타났다. 5일군에서는 이러한 신경섬유의 밀도와 분지가 증가하여 상피하층으로 더욱 가까이 연장되었고 여러개의 varicosities도 관찰되었다. 10일군부터 신경섬유의 분지 및 밀도가 증가하였고 상피하층에서 신경총을 관찰할 수 있었으며 상피내 신경섬유의 진입을 관찰할 수 있었다. 20일군에서는 증가된 상피층내 신경섬유를 관찰할 수 있었으나 25일군에서부터 전반적인 신경섬유의 분포 및 밀도가 많이 감소되었으며 성장이 완료된 군에서는 chemosensory corpuscle 지역을 제외하고는 신경섬유를 거의 관찰할 수 없었다. The purpose of this study was to investigate the distribution of nerves in the incisive papilla of rat by each developing stage. The distribution on nerves in the incisive papilla was investigated by means of immunohistochemistry using an antibody against calcitonin gene related peptide (CGRP). The results were as follows : At 1 day after birth, CGRP-immunoreactive(IR) nerve fibers first appeared in loose connective tissue without forming an apparent terminal structure. At 5 days, the CGRP-IR nerve fibers increased in density and extended more to subepithelium. CGRP-IR nerve fiber began to penetrate into the epithelium at 10 days and reached maximum at 20 days. At 25 days, intraepithelial nerve fibers were not found, and the distribution and density of nerve fiber began to decrease. In adult rat, the nerve fiber almost could not be found in dome region except in chemosensory corpuscle region.

Malachite Green유도체의 합성과 그 항균성에 관한 연구

金浩植,金鍾大,金在根,韓文洙 嶺南大學校附設 基礎科學硏究所 1986 基礎科學硏究 Vol.6 No.-

Malachite Green derivative bearing butyl group was prepared in the form of Leuco base, 4-butyl-4'. 4"-bis(dimethylamino) triphenylmethane, by the condensation reaction of N,N-dimethylaniline with the formyl derivative of butylbenzene, which had been obtained from butylbenzene by chlormethylation followed by oxidation. The butylbenzene was prepared by the alkylation reaction of benzene with n-butyl choride which had been obtained from n-butyl alcohol and zinc chloride. The antimicrobial and antifungal activity of buty-Malachite Green was evaluated in terms of minimum inhibitory concentration by the dilution method. The butyl-Malachite Green showed considerably increasing activity. This activity was stronger than that of hexyl-Malachite Green. This phenomenon suggested that the activities of the alkyl derivatives increase with decrease in the number of carbon in the alkyl group.

출생후 성장발육에 따른 흰쥐 유곽유두의 CGRP 면역양성 신경분포변화

김현정,이재문,배용철,김영진 大韓小兒齒科學會 1997 大韓小兒齒科學會誌 Vol.24 No.2

출생 후 성장 발육에 따른 흰쥐 유곽유두의 신경분포 및 변화를 관찰하기 위하여 생후 1,5,10,15,20,25,35일 및 완전히 성장한 흰쥐를 개상으로 calcitonin gene-related peptide(CGRP)를 이용한 면역조직화학적 방법으로 관찰하여 다음과 같은 결과를 얻었다. CGRP-면역양성 신경섬유들이 혀의 양측에서부터 유두 내로 진입하여 성장발육에 따라 그 분포밀도가 중가하다가 10일군부터는 상피의 직하방에 특히 풍부하게 분포하여 subepithelial plexus를 형성하고 있었다. 1일군에서는 CGRP-면역양성 신경섬유의 특징적인 양상인 염주알 모양이 명확히 관찰되었으나 그 이후로는 염주알 모양은 다소 감소하였고 하나의 선으로 보이는 경우도 있었다. 상피 내로 진입하는 CGRP-면역양성 신경섬유들은 5일군에서 드물게 관찰되었으며 taste pore를 가진 성숙한 미뢰가 처음으로 관찰되는 10일군에서 그 수가 증가하여 출생 후 15일군에서는 상피 전반에 걸쳐 풍부하게 관찰되었다. 그러나 그 이후 20일군부터는 미뢰가 존재하는 trench wall의 하방 2/3부위의 CGRP-면역양성 신경섬유는 감소하고 유두의 top surface와 trench wall의 상방 1/3에 CGRP-면역양성 신경섬유가 제한적으로 관찰되었다. The purpose of this study was to investigate the distribution of nerves in the rat circumvallate papilla by each developing stage. The distribution of nerves in the rat circumvallate papilla were investigated by means of immunohistochemistry for detection of calcitonin gene-realted peptide(CGRP). The results were as follows: CGRP-immunoreactive(IR) nerve fibers entered the base of the papilla laterally to form a subepithelial plexus. On the 1st postnatal day, the bead-like appearance, typical appearance of CGRP-IR nerve fiber, was seen, but from the 5th postnatal day the bead-like appearance was seen less clearly and nerve fibers looking like a line were often observed. Mature taste buds having taste pores were first seen at the 10th postnatal day and then, increased markedly after this stage. CGRP-IR nerve fibers entering the epithelium were rarely seen on the 5th postnatal day but they increased in number on the 10th postnatal day when mature taste buds having taste pores were firstly observed and then on the 15th postnatal day, they were observed abundantly throughout entire epithelium. But from the 20th postnatal day, the nerve fibers in the lower two-third of the trench wall in which taste buds exist decreased but on the top surface and upper one-third of the trench wall the nerve fibers were obsered limitedly.

整流方式에 따른 醫療用 X-線 裝置의 特性

김재문,권덕문,최세곤 嶺南大學校 工業技術硏究所 1995 工業技術硏究所論文集 Vol.23 No.2

The output characteristics on various rectification methods of X-ray equipments for the purpose of medical use are investigated by means of X-ray multimeter. in comparison the measured values with setting values, the accuracy of X-ray tube voltage and illumination time was varied in the range of +70% in case of three phase type including inverter type. The inverter type also showed a good reproducibility. As the experimental results, it is desirable that the inverter type X-ray equipment will be widely used in the near future.

3상 농형유도기를 사용한 자여식 단상유도발전기의 속도특성

김재민,홍문석,진충민,좌종근 濟州大學校 産業技術硏究所 1999 산업기술연구소논문집 Vol.10 No.2

The speed characteristics of self-excited single-phase induction Generator using Three-Phase Squirrel-Case Induction Machine which has only two series capacitors with respect to the load are analyzed in this paper. The symmetrical component method and the condition of equivalent circuit are applied to develop an equivalent model of this generator. The speed characteristics of this generator are computed by using this model and compared with those of the self-regulated generator.

스키상해에 관한 실태 조사 연구

김정주,민경두,이재문 경희대학교 체육과학연구소 2001 體育學論文集 Vol.29 No.-

As the number of people who enjoy skiing are increasing, it has become more important to work out a countermeasure for prevention of injuries. To prevent accidents, finding out the causes and analyzing them is more meaningful than post-accidental treatment. This study is based on records of 494 injured people at H ski resorts. From December, 2000 to March, 2001 this research and analysis was undertaken in detail by classifying the situation and causes at the accidents, into personal, material and environmental factors. The results from which following conclusion were made. First of all, after analyzing the situation of the injured people, the facts that most of the accidents occurred on weekends and holidays, when most students and salarymen went skiing and that the injures were usually in the form of a slight contusion were found out. The major cause was that people ignored the safety rules and descended immoderately. But skiers that used a certain ski resort regularly showed lower occurrence of injuries. For these reasons in order to reduce the rate of accidents happening skiers should be tested by a ski instructor and then should be taught at a right level. Ski-related organizations should inform skiers with facts that are commonly misunderstood through seminars and training courses. The lack of facilities for beginners should be improved and resorts for group training courses should be built separately so that beginners can prevent ahead of time, form getting hurt in a disorderly situation .