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      • Basic, HCC basic : O-031 ; Angiotensin-II-induced anti-apoptotic signaling in hepatocellular carcinoma and hepatic stellate cells

        ( Jung Hee Kwon ), ( Eun Ju Cho ), ( Min Sun Kwak ), ( Eun Sun Jang ), ( Yun Bin Lee ), ( Jeong Hoon Lee ), ( Su Jong Yu ), ( Yoon Jun Kim ), ( Hyo Suk Lee ), ( Jung Hwan Yoon ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.1

        Background: Angiotensin II (Ang-II) may activate antiapoptotic signaling via NADPH oxidase-1 (NOX-1) in hepatic stellate cells (HSCs), and thereby participate in hepatic fibrosis. This study was to evaluate whether Ang-II may also induce this signaling in TRAIL-induced apoptosis in hepatocellular carcinoma (HCC) cells as well as in HSCs, and to search for agents which can overcome this signaling in these cells Methods: Human HSC (LX-2) and HCC cell lines (SNU-761) were used in this study. TRAIL, flavopiridol and deoxycholic acid (DC) were used to induce apoptosis. Cell growth was assessed using the MTS assay and the apoptotic cell death was measured by DAPI staining. Apoptotic signaling cascades were explored by immunoblot analysis. To reduce cellular protein expressions, specific small interfering RNA (siRNA) was transfected into cells. Results: Ang-II attenuated TRAIL-induced apoptosis in both HCC and HSC cells. NOX-1 siRNA transfection reversed Ang-II-induced attenuation of TRAIL-induced apoptosis. In HCC cells, DC induced apoptosis more efficiently in the presence of Ang-II than in the absence of Ang-II, and this was associated with more prominent activation of JNK. In HSC cells, flavopiridol induced apoptosis irrespective of the presence or absence of Ang-II. Moreover, sub-lethal dose of flavoprirdol significantly attenuated TGF-beta induced smooth muscle actin and vimentin induction in HSC cells, and this was associated with reduced activation of p42/44 MAPK following TGF-beta treatment independent of Smad activation. Conclusions: These results demonstrate that Ang-II attenuates TRAIL-induced apoptosis in HCC and HSC cells via NOX-1. Bile acid and flavopiridol may overcome this Ang-II signaling in HCC and HSC cells, respectively, and therefore, may therapeutically be implicated in cirrhotic HCC patients with elevated Ang-II signaling.

      • KCI등재

        Identification of a Proper Phytoavailable Arsenic Extraction Method Associated with Arsenic Concentration in Edible Part of three Crops in Soils Near Abandoned Mining Areas

        Yoon,,Jung-Hwan,Kim,,Young-Nam,Lee,,Dan-Bi,Kim,,Kwon-Rae,Kim,,Won-Il,Kim,,Kye-Hoon 한국토양비료학회 2017 한국토양비료학회지 Vol.50 No.6

        This study aimed to investigate correlations between concentrations of extractable Arsenic (As) with varying chemical solutions (0.1 M $Ca(NO_3)_2$, 0.1 M $(NH_4)2HPO_4$, 0.5 M EDTA, Mehlich 3, and 0.5 M $NaHCO_3$) and those of As in crops, and then to seek the most suitable soil extraction method for predicting the potential of As uptake in crops cultivated in soils contaminated with As. For a mesocosm experiment, pepper (Capsicum annuum L.), soybean (Glycine max L.), and rice (Oryza sativa L.) were cultivated for three months in pots containing soils taken from the arable areas near abandoned mines in Korea. Following the cultivation, soil pH and DOC significantly increased by treatments of lime and lime plus compost, respectively, while insignificant influences in changing total and all extractable As concentrations were found in all soils. Arsenic concentration in edible part of all crops considerably depended on the extractable As concentration in the soils, particularly with Mehlich 3. All extractable As concentrations in the soils of C. annuum and G. max were significantly correlated with As concentration in their edible parts. For O. sativa, the extractable concentrations of Mehlich 3 ($R^2$: 0.18 at p: 0.006) and EDTA ($R^2$: 0.11 at p: 0.036) showed only marked relationships with As concentration in the edible part. These results may indicate that the Mehlich 3 and EDTA are soil extractants to determine phytoavailable As in soil that provide better prediction for As transfer from soil to crop.

      • KCI등재SCOPUS

        Oxidative modification of ferritin induced by hydrogen peroxide

        ( Jung Hwan Yoon ), ( Sung Ho An ), ( Inn Goo Kyeong ), ( Myeong Seon Lee ), ( Sang Chul Kwon ), ( Jung Hoon Kang ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.3

        Excess free iron generates oxidative stress that may contribute to the pathogenesis of various causes of neurodegenerative diseases. In this study, we assessed the modification of ferritin induced by H2O2. When ferritin was incubated with H2O2, the degradation of ferritin L-chain increased with the H2O2 concentration whereas ferritin H-chain was remained. Free radical scavengers, azide, thiourea, and N-acetyl-L-cysteine suppressed the H2O2-mediated ferritin modification. The iron specific chelator, deferoxamine, effectively prevented H2O2-mediated ferritin degradation in modified ferritin. The release of iron ions from ferritin was increased in H2O2 concentration-dependent manner. The present results suggest that free radicals may play a role in the modification and iron releasing of ferritin by H2O2. It is assumed that oxidative damage of ferritin by H2O2 may induce the increase of iron content in cells and subsequently lead to the deleterious condition. [BMB reports 2011; 44(3): 165-169]

      • SCIESCOPUSKCI등재후보

        Oxidative modification of ferritin induced by hydrogen peroxide

        Yoon,,Jung-Hwan,An,,Sung-Ho,Kyeong,,Inn-Goo,Lee,,Myeong-Seon,Kwon,,Sang-Chul,Kang,,Jung-Hoon Korean Society for Biochemistry and Molecular Biol 2011 BMB Reports Vol.44 No.3

        Excess free iron generates oxidative stress that may contribute to the pathogenesis of various causes of neurodegenerative diseases. In this study, we assessed the modification of ferritin induced by $H_2O_2$. When ferritin was incubated with $H_2O_2$, the degradation of ferritin L-chain increased with the $H_2O_2$ concentration whereas ferritin H-chain was remained. Free radical scavengers, azide, thiourea, and N-acetyl-$_L$-cysteine suppressed the $H_2O_2$-mediated ferritin modification. The iron specific chelator, deferoxamine, effectively prevented $H_2O_2$-mediated ferritin degradation in modified ferritin. The release of iron ions from ferritin was increased in $H_2O_2$ concentration-dependent manner. The present results suggest that free radicals may play a role in the modification and iron releasing of ferritin by $H_2O_2$. It is assumed that oxidative damage of ferritin by $H_2O_2$ may induce the increase of iron content in cells and subsequently lead to the deleterious condition.

      • KCI등재

        Epidemiological and Clinical History of Viral Hepatitis in Korea

        Yoon,Jung-Hwan,Cho,Se,Hyun,Kim,Do,Young,Yu,Su,Jong,Han,Kwang-Hyub 대한감염학회 2021 Infection and Chemotherapy Vol.53 No.1

        Viral hepatitis is the most important cause of acute and chronic liver disease in Korea. Particularly, hepatitis B virus (HBV) is the leading cause of liver-related mortality. Because of the nationwide vaccinations in the 1980s, hepatitis B surface antigen positive rates substantially decreased from 8% to 3%. Moreover, the introduction of potent nucleoside or nucleotide analogs led to the effective treatment of patients who had already been infected by HBV. The remaining issue has been to develop novel drugs that can cure HBV infection. Hepatitis C virus (HCV), on the other hand, is a hepatotropic virus that is parenterally transmitted. In Korea, the prevalence of HCV is estimated to be approximately 1%. Although no effective vaccine for HCV has been developed yet, highly effective and safe direct-acting antiviral therapy, which has a short treatment duration of 8 - 12 weeks, has made HCV eradication possible globally. Currently, the unsolved issue regarding HCV management is low disease awareness among patients and health care providers. Therefore, nationwide testing for anti-HCV would be a solution to identify patients infected with HCV but with no symptoms. Lastly, the Hepatitis A virus (HAV) is orally transmitted and results in acute hepatitis. In Korea, the young adult population is a high-risk group since this group is not vaccinated against HAV. More active vaccination and improved hygiene would be necessary to prevent HAV infection.

      • SCIE

        TNF-$\alpha$ and TNF-$\beta$ polymorphisms with susceptibility to gastric cancer in a Korean population

        Yoon,,Jung-Hwan,Song,,Jae-Hwi,Kang,,Young-Hwi,Park,,Yong-Kyu,Cao,,Zhang,Nam,,Suk-Woo,Lee,,Jung-Young,Park,,Won-Sang 대한독성유전단백체학회 2010 Molecular & cellular toxicology Vol.6 No.2

        Tumor necrosis factors (TNF) regulate the inflammatory response, cell growth and differentiation and its genetic polymorphisms are correlated with various cancer types including colon, and ovarian cancer. To determine whether polymorphisms at TNFA -G308A and TNFB +G252A were associated with a susceptibility to gastric cancer, we investigated the genotype and allele frequencies of these genes in 181 gastric cancer patients and 290 healthy individuals. The polymorphism analysis was performed by amplifying the promoter region of TNF-$\alpha$ for TNFA -308 and the first intron of TNF-$\beta$ for TNFB +252, digestion with NCOl restriction enzymes, and then sequencing the products. The frequencies of the genotypes for TNFA -308 were G/G, G/A, and A/A, 93.4% (169/181), 5.0% (9/181) and 1.7% (3/181), respectively, in gastric cancer patients and 88.6% (257/290), 11.4% (33/290) and 0% (0/290), respectively, in the healthy controls. Statistically, there was a significant difference in the genotype frequency of the TNFA -308 between the healthy controls and the gastric cancer patients (P=0.0034). However, genotype and allele frequency for TNFB +252 was not associated with an increased risk of gastric cancer in this population. When stratified by histological subtype of gastric cancer, there was no statistical significance between the risk and the two polymorphisms of TNFA -308 and TNFB +252. Our findings suggest that the TNFA -308 polymorphism of the TNF-$\alpha$ gene may be closely associated with susceptibility to gastric cancer in Korean patients.

      • KCI등재

        Identification of a Proper Phytoavailable Arsenic Extraction Method Associated with Arsenic Concentration in Edible Part of three Crops in Soils Near Abandoned Mining Areas

        Jung-Hwan,Yoon,Young-Nam,Kim,Dan-Bi,Lee,Kwon-Rae,Kim,Won-Il,Kim,Kye-Hoon,Kim 한국토양비료학회 2017 한국토양비료학회지 Vol.50 No.6

        This study aimed to investigate correlations between concentrations of extractable Arsenic (As) with varying chemical solutions (0.1 M Ca(NO3)2, 0.1 M (NH4)2HPO4, 0.5 M EDTA, Mehlich 3, and 0.5 M NaHCO3) and those of As in crops, and then to seek the most suitable soil extraction method for predicting the potential of As uptake in crops cultivated in soils contaminated with As. For a mesocosm experiment, pepper (Capsicum annuum L.), soybean (Glycine max L.), and rice (Oryza sativa L.) were cultivated for three months in pots containing soils taken from the arable areas near abandoned mines in Korea. Following the cultivation, soil pH and DOC significantly increased by treatments of lime and lime plus compost, respectively, while insignificant influences in changing total and all extractable As concentrations were found in all soils. Arsenic concentration in edible part of all crops considerably depended on the extractable As concentration in the soils, particularly with Mehlich 3. All extractable As concentrations in the soils of C. annuum and G. max were significantly correlated with As concentration in their edible parts. For O. sativa, the extractable concentrations of Mehlich 3 (R2: 0.18 at p: 0.006) and EDTA (R2: 0.11 at p: 0.036) showed only marked relationships with As concentration in the edible part. These results may indicate that the Mehlich 3 and EDTA are soil extractants to determine phytoavailable As in soil that provide better prediction for As transfer from soil to crop.

      • HBV : PE-014 ; Anti-viral effect of telbivudine plus adefovir in patients with lamivudine-resistant chronic hepatitis B not achieving complete virologic response to lamivudine plus adefovir

        ( Jung Hee Kwon ), ( Eun Ju Cho ), ( Yun Bin Lee ), ( Jeong Hoon Lee ), ( Su Jong Yu ), ( Jung Hwan Yoon ), ( Hyo Suk Lee ), ( Yoon Jun Kim ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.-

        Background: Little is known about the optimal rescue therapy for patients with lamivudine-resistant chronic hepatitis B (CHB) showing persistent HBV DNA during lamivudine plus adefovir treatment. We evaluated whether telbivuidne plus adefovir could be an option as a rescue therapy for these patients. Methods: We assessed 43 patients who were switched from lamivudine plus adefovir to telbivudine plus adefovir for lamivudine-resistant CHB in a single institution from July 2010 to December 2010. We measured serum HBV DNA by real time PCR before and at 3, 6, 9, and 12 months after treatment Results: The baseline HBV DNA level was 3.24±1.47 log10 IU/ml. The rate of primary non-response (HBV DNA reduction <2 log10 IU/ml at 6 months) was 71.4% (30/42). At 12 months after treatment, only 26.3% (10/38) of the patients achieved complete virologic response. Partial response and inadequate response rates were 5.3% (2/38) and 68.4% (26/38), respectively. HBeAg clearance was observed in only 2 out of the 18 HBeAg positive patients. Mean DNA reductions at 6 and at 12 months were -0.96 log10 IU/ml and -1.01 log10 IU/ml, respectively. Virologic breakthrough was found in 2 patients. The baseline HBV DNA levels were significantly lower in patients who achieved complete virologic response at 12 months than in those who showed partial or inadequate responses (1.80±0.44 vs.3.75±1.47 log10 IU/ml, p<0.05). Conclusions: Telbivudine plus adefovir was not effective as a rescue treatment in patients with lamivudine-resistant chronic hepatitis B showing no complete virologic response to lamivudine plus adefovir. More potent anti-viral treatment may be needed in these patients, although telbivudine plus adefovir might be an option in patients with low baseline HBV DNA levels.

      • SCOPUSKCI등재
      • HBV : PE-014 ; Anti-viral effect of telbivudine plus adefovir in patients with lamivudine-resistant chronic hepatitis B not achieving complete virologic response to lamivudine plus adefovir

        ( Jung Hee Kwon ), ( Eun Ju Cho ), ( Yun Bin Lee ), ( Jeong Hoon Lee ), ( Su Jong Yu ), ( Jung Hwan Yoon ), ( Hyo Suk Lee ), ( Yoon Jun Kim ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.1

        Background: Little is known about the optimal rescue therapy for patients with lamivudine-resistant chronic hepatitis B (CHB) showing persistent HBV DNA during lamivudine plus adefovir treatment. We evaluated whether telbivuidne plus adefovir could be an option as a rescue therapy for these patients. Methods: We assessed 43 patients who were switched from lamivudine plus adefovir to telbivudine plus adefovir for lamivudine-resistant CHB in a single institution from July 2010 to December 2010. We measured serum HBV DNA by real time PCR before and at 3, 6, 9, and 12 months after treatment Results: The baseline HBV DNA level was 3.24±1.47 log10 IU/ml. The rate of primary non-response (HBV DNA reduction <2 log10 IU/ml at 6 months) was 71.4% (30/42). At 12 months after treatment, only 26.3% (10/38) of the patients achieved complete virologic response. Partial response and inadequate response rates were 5.3% (2/38) and 68.4% (26/38), respectively. HBeAg clearance was observed in only 2 out of the 18 HBeAg positive patients. Mean DNA reductions at 6 and at 12 months were -0.96 log10 IU/ml and -1.01 log10 IU/ml, respectively. Virologic breakthrough was found in 2 patients. The baseline HBV DNA levels were significantly lower in patients who achieved complete virologic response at 12 months than in those who showed partial or inadequate responses (1.80±0.44 vs.3.75±1.47 log10 IU/ml, p<0.05). Conclusions: Telbivudine plus adefovir was not effective as a rescue treatment in patients with lamivudine-resistant chronic hepatitis B showing no complete virologic response to lamivudine plus adefovir. More potent anti-viral treatment may be needed in these patients, although telbivudine plus adefovir might be an option in patients with low baseline HBV DNA levels.

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