노화가 인체 중간엽 줄기세포로부터 조골세포로의 증식 및 분화에 미치는 영향

백기현,태현정,오기원,이원영,조정기,권순용,강무일,차봉연,이광우,손호영,강성구,김춘추 대한내분비학회 2003 Endocrinology and metabolism Vol.18 No.3

연구배경: 일반적으로 골다공증과 연관된 위험인자로는 연령, 폐경, 약물, 불충분한 칼슘섭취, 만성질환 및 운동부족 등이 있는데, 특히 노화가 진행할수록 골밀도가 감소하는 것은 잘 알려져 있다. 노화와 관련하여 진행되는 골소실은 조골세포 및 전구조골세포의 기능적 결핍에 의한 골형성의 감소가 주요한 요인으로 여겨지고 있다. 그 동안 연령이 조골모 세포의 양과 조골모 세포로부터 성숙조골세포로의 분화 및 증식에 미치는 영향에 대한 일부 보고들이 있었으나 아직 일치된 견해는 없는 형편이다. 방법: 다양한 연령의 사람으로부터 골수를 채취, 중간엽 줄기세포가 포함된 단핵세포를 분리한 후 조골세포로 분화하기 좋은 조건하에서 배양하였다. 대상군은다시 젊은군과 노령군으로 구분하여 다양한 변수를 비교 분석하였다. 일차배양에서는 CFU-F를 계수하여 골수내 중간엽 줄기세포의 수를 추산하였고, 칼슘측정을 통하여 기질의 무기화 정도를 비교하였다. 계대배양후 이차배양에서는 시기별로 알카리성 포스파타제 활성도를 측정하고 오스테오칼신 mRNA의 발현을 관찰하여 젊은군과 노령군 사이의 증식능 차이를 비교하였다. 또한 이차배양 시기별로 MTT 측정을 하여 양군간에 증식능 차이가 있는지 알아보았다. 결과: 1. 일차배양 15일째에 평균 CFU-F의 수는 젊은군에서 유의하게 많았다(젊은군 148.3±28.9, 노령군 54.3±9.1, p=0.02). CFU-F의 평균면적은 젊은군에서 넓은 경향을 보였으나 통계적으로 유의하지는 않았다. 2. 일차배양 17일 경과 후 양군간에 기질 칼슘 침착정도는 유의한 차이를 보이지 않았다(젊은군 103.6±50.6, 노령군: 114.0±56.5, p=NS). 3. 이차배양 10일째에 젊은군에서 알카리성 포스파타제 활성도가 고령군에 비해 유의하게 높았다(젊은군: 935.5±115.0 U/mg, 노령군: 578.4±115.7U/mg,p.0.05). 고령군에서는 시간 경과에 따른 변화가 미약했으며 전반적으로 알카리성 포스파타제의 활성도가 젊은군에 비해 낮았다. 4. 이차배양도중 오스테오칼신 mRNA의 발현은 배양시기별로 젊은군에 비해 고령군에서 더 낮은 경향을 관찰할 수 있었다. 5. 이차배양 10일과 15일에 젊은군에서 노령군보다세포증식이 유의하게 증가된 양상을 보였다(10앓 젊은군 0.73±0.05, 노령군 0.58±0.04, p=0.05, 15일; 젊은군 0.80±0.05, 노령군 0.70±0.03, p=0.05).결론: 이상의 연구에서 저자들은 노령군에서 젊은군보다 골수 내 중간엽줄기세포의 수가 적고, 노령군에서 유래한 전구조골세포의 성숙조골세포로의 증식 및 분화가 젊은군 보다 감소해 있는 것을 관찰할 수 있었다. Background: Osteoblasts originate from osteoprogenitor cells in bone marrow stroma, termed mesenchymal stem cells (MSCs) or bone marrow stromal cells. Each MSC forms colonies (colony forming units-fibroblasts [CFL-Fs]) when cultured ex vivo. There are some reports about the age-related changes of the number and osteogenic potential of osteoprogenitor cells, but any relationship has not been clearly established in humans. In this study, we counted MSCs using CFU-Fs count and examined the proliferative capacity and differentiation potential of osteoprogenitor cells. Finally, we analyzed how these parameters varied with donor age. Methods: Bone marrow was obtained from the iliac crest of young (n=6, 27.2±8.6 years old) and old (n= 10, 57.4k6.7 years old) healthy donors. Mononuclear cells, including MSCs, were isolated and cultured in osteogenic medium. In primary culture, we compared the colony-forming efficiency of MSCs between the two groups and determined the matrix calcification. When primary culture showed near confluence, the cells were subcultured. Alkaline phosphatase activity, osteocalcin expression by RT-PCR and proliferative potential by MTT assay were examined by the time course of secondary culture. Results: At the 15th day of primary culture, the mean number of CFU-Fs was significantly higher in the younger donors (young: 148.3±28.9, old: 54.3±9.1, p=0.02) and the mean size of CFL-Fs was also larger in the younger donors than the older donors. However, matrix calcification was not different between the two groups (young: 103.6±50.6, old: 114.0±56.5, p=NS). In secondary culture, alkaline phosphatase activities were significantly lower in the older donors. The younger donors showed peak alkaline phosphatase activity at day 10, while the older donors didn't showed a remarkable peak (young: 935.5±115.OU/mg, old: 578.4±115.7U/mg, p<0.05). Total cell number as a proliferative index increased progressively during the secondary culture and a significantly greater cell number was noted in the younger donors. Osteocalcin expression was generally upregulated in the younger donors, but this was not statistically significant. Conclusion: Our study shows that the number of osteoprogenitor cells is decreased during aging and that the proliferative capacity and differentiation potential of osteoprogenitor cells seem to be reduced during aging (J Kor SOC Endocrinol 18:296-305, 2003).

연준모치,Phoxinus phoxinus 와 금강모치, Rhynchocypris kumgangensis의 서식지 분리와 먹이 선택

백현민,송호복,심하식,김영건,권오길 江原大學校 附設 環境硏究所 2002 環境硏究 Vol.19 No.-

The authors investigated habitat segregation and prey selectivity of two cohabitants, Phoxinus phoxinus and Rhynchocypris kumgangensis, in Dongdaesheon, Jeongsoen-gun, Kangwon-do, Korea, from April to October 2001. P. phoxinus dominated the upper reaches of the stream, whereas R. kumgangensis dominated the middle and lower reaches of the upprer stream. P.phoxinus inhabited zones of relatively slow water velocity in the bottoom layer, but R. kumgangensis lived zones of relatively high water velocity in the middle and upper layers of the cohabitat. P. phoxinus ingested mainly Ephemeroptera, Trichophtera, Diptera in spring, and Diptera in summer and autumn. R. kumgangensis fed mainly on terrestrial insects in spring and summer, and Diptera in autumn.

후직장의 위장관 간질 종양 1예

최영일,이승현,안병권,백승언 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.2

The retrorectal gastrointestinal stromal tumor is very rare. An 24-year-old woman patient was admitted for huge pelvic mass with constipation, lower abdominal discomfort. The pelvic mass was palpable on posterior rectal wall on digital rectal examination. On exploration, tumor excision was performed. Gross finding shows 14x11x10.5 cm sized grayish tan and irregular mass. The cut surface is solid, bright yellow to grayish yellow with foci of hemorrhage and calcification. The tumor show spindle shaped cells and mitosis. It showed positive staining for c-kit. The tumor was diagnosed as gastrointestinal stromal tumor in rectrorectal space.

연준모치, Phoxinus phoxinus와 금강모치, Rhynchocypris kumgangensis의 서식지 분리와 먹이 선택

백현민,송호복,심하식,김영건,권오길 한국어류학회 2002 韓國魚類學會誌 Vol.14 No.2

강원도 정선군의 동대천에 동서하고 있는 Phoxinus phoxinus와 Rhynchocypris kumgangensis의 서식지 분리와 먹이 선택에 대하여 2001년 4월부터 9월까지 계절별로 조사, 연구하였다. 두 종은 전형적인 Aa형 하천에 서식하고 있었으며, 상류쪽에서는 P. phoxinus가, 중·하류쪽에서는 R. kumgangensis가 우세하였고, 수온이 중요한 서식 제한 요인으로 나타났다. 동일 서식지에서 P. phoxinus는 유속이 느린 곳에, R. kumgangensis는 상대적으로 유속이 빠른 곳에서 점유율이 높게 나타났으며, P. phoxinus는 하천의 하층에, R. kumgangensis는 상층 및 중층에 주로 서식하였다. 먹이 생물은 P. phoxinus는 봄에 Ephemeroptera, Trichoptera, Diptera를 주로 섭식하였고 여름과 가을에는 Diptera를 섭식하였으며, R. kumgangensis는 봄과 여름에는 육상곤충, 가을에는 Diptera를 주로 섭식하였다. The authors investgated habitat segregation and prey selectivity of two cohabitants, Phoxinus phoxinus and Rhynchocypris kumgangensis, in Dongdeacheom, Jeongsoen-gun, Kangwon-do, Korea, from April to October 2001. P. phoxinus dominated the upper reaches of the stream, whereas R. kumgangensis dominated the middle and lower reaches of the upprer stream. P. phoxinus inhabited zones of relatively slow water velocity in the bottom layer, but R. kumgangensis lived zoned of relatively high water velocity in the middle and upper layers of the cohabitat. P. phoxinus ingested mainly Ephemeroptera, Trichoptera, Diptera in spring, and Diptera in summer and autumn. R. kumgangensis fed mainly on terrestrial insects in spring and summer, and Diptera in autumn.

Zoledronic acid가 골육종 세포에서 Matrix Metalloproteinase-2와 -14의 발현과 폐전이에 미치는 영향

김용백,김상범,권영호,김재도 KOSIN UNIVERSITY COLLEGE OF MEDICINE 2006 高神大學校 醫學部 論文集 Vol.21 No.1

Purpose : Gelatinase A (MMP-2) and membrane-type metalloproteinase (MT1-MMP, MMP-14) play a critical role in metastasis. It was known that bisphosphonate acts not only to protect bone absorption but also to inhibit tumor invasion and metastasis. The aim of this study is to investigate the inhibition of MMP-2 and 14 in osteosarcoma by zoledronic acid, whether it can prevent lung metastasis in nude mouse. Material and Method : Protein synthesis and mRNA expression of MMP-2 and MMP-14 cultured human MG-63 osteosarcoma and HOS osteosarcoma cell lines with zoledronic acids were measured. In vivo test, it was observed that the inhibitory effect of zoledronic acid on lung metastasis of osteosarcoma with nude mouse model. Result : Zoledronic acid inhibited mRNA expression of MMP-14 and MMP-2 in cultured human MG-63 osteosarcoma and HOS osteosarcoma cell lines. Zoledronic acid decreased the intensity of activated 62KD MMP-2 in human MG-63 osteosarcoma and HOS osteosarcoma cell lines on gelatine zymography. Number of metastatic lung nodule decreased from 9.4 to 1.2 in MG-63 osteosarcoma cell line and from 8 to 1.6 in HOS osteosarcoma cell line with nude mouse model. Conclusion : In vitro, zoledronic acid had an inhibitory effect on the MMP-14 and MMp-2, which also prevents lung metastasis of osteosarcoma in vivo. So, zoledronic acid could be used as one of good chemotherapeutic agents against metastasis of osteosarcoma.

Productivity S/W 학습용 웹 코스웨어에서 상황맥락적 오류교정 패드백이 학업성취도에 미치는 영향

김도윤,배영권,백장현,이태욱 한국컴퓨터교육학회 2004 컴퓨터교육학회 논문지 Vol.7 No.1

현재 웹 코스웨어에서의 형성평가와 이에 대한 피드백 시스템은 많이 구축되어 있다. 그러나 대부분 웹 코스웨어의 형성평가와 사용자 반응에 따라 제공되는 피드백은 실제의 문제 상황과 거리가 먼 상태에서 단순한 텍스트 형태로 정오의 표시, 정답 표시, 관련 정보의 표시 등을 제공하고 있다. 이는 자칫 피드백의 교정적 기능을 약화시켜 학습자의 학습 내용에 대한 이해와 학습 전이의 가능성을 감소시킬 수 있다. 또한 구성주의 학습이론에 따르면 학습은 그 것이 발생하는 상황에 영향을 받고 지식이 어떤 맥락에서 학습했느냐에 따라 다르게 학습되며 전이된다고 한다. 이런 배경에서 본 연구는 웹 코스웨어에서 형성평가를 실시할 때 학습자의 반응에 따라 피드백을 문제 상황과 밀접하고 유사하게 상황맥락적으로 제시할 수 있는 상황맥락적 오류고정 피드백시스템을 설계하고 구현하였다. 그리고 실제 Productivity S/W를 학습할 수 있는 웹 코스웨어에 ‘정오/정답제공형 피드백', ‘관련정보제공형 피드백’‘상황맥락적 오류교정 피드백’을 적용한 후 ‘상황맥락적 오류교정 피드백’이 학업 성취에 있어서 다른 두 가지 유형의 피드백보다 더 효과적인지를 검증하였다. Today there are many Web courseware systems for formative evaluation and feedback. Formative evaluation and feedback provided according to users’ response in most Web courseware systems, however, are simple texts showing only whether correct or wrong, correct answers, relevant information, etc., far deviated from actual context. Thus such a system may weaken the corrective function of feedback and, as a result, reduce learners’ understanding of contents and the possibility of leaming transfer. In addition, according to the learning theory of constructivism, learning is influenced by the situation, in which it happens, and knowledge is learned and transferred differently depending on the context in which it is learned. In the background, this study designed and implemented a contextual error-correction feedback system that can provide feedback in a context closely related and similar to the relevant situation according to the response of learners when formative evaluation is carried out in Web courseware. In addition, it applied ‘correction/correct-answer-providing feedback’, ‘relevant information providing feedback’ and ‘contextual error-correction feedback’ to Web courseware for learning actual productivity S/W and verified if ‘contextual error-correction feedback’ is more effective than other two types of feedback for learners’ academic achievement.

좌상후 말초신경의 조직학적 변화

정소학,김성대,권영호,손정환,백선용 고신대학교 의학부 2000 高神大學校 醫學部 論文集 Vol.15 No.1

Background The morphological changes in the peripheral nerve and S-100 immunoreactivity were studied after axotomy. Methods Adult Sprague-Dawley rats received crush injury with aneurysmal clip in the sciatic nerve at mid-thigh level. The animals were cardiac perfused with 4% paraformaldehyde for cryostat section and half Karnovsky fixative for semithin section from 6 hours to 4 weeks after crush injury. The immunohistochemistry of Schwan cells was performed. Results Degeneration of myelin sheath and endoneurial edema were observed at 6 hours. Macrophages were invaded into the basal lamina tube and phagocytosed the destructed axons and myelin sheath at 3 days. Proliferation of Schwann cells in the basal lamina tube was observed at 5 days group. Thin myelinated nerve fiber and degeneration of myelin sheath were observed at 2 weeks. Conclusion These results indicate that after crush injury of the peripheral nerve regeneration of myelin sheath and Schwan cells is partially completed in 1 week.

상추(Lactuca sativa L.)의 ACAT 억제 Diterpenoid, Phytol : 식용 식물자원으로부터 활성물질의 탐색 Development of Biologically Active Compound from Edible Plant Sources

장태오,방면호,송명종,홍윤희,김지영,정대균,배동근,권병목,김영국,이현선,김인호,백남인 한국농화학회 2003 Applied Biological Chemistry (Appl Biol Chem) Vol.46 No.1

Decrease in glucose transporter 1 levels and translocation of glucose transporter 3 in the dentate gyrus of C57BL/6 mice and gerbils with aging

Kwon Young Lee,Dae Young Yoo,Hyo Young Jung,Loktam Baek,Loktam Baek,Hangyul Lee,Hyun Jung Kwon,Jin Young Chung,Seok Hoon Kang,Dae Won Kim,In Koo Hwang,Jung Hoon Choi 한국실험동물학회 2019 한국실험동물학회 학술발표대회 논문집 Vol.2019 No.1

Clinical predictors of chest radiographic abnormalities in young children hospitalized with bronchiolitis: a single center study

Kim, Ga Ram,Na, Min Sun,Baek, Kyung Suk,Lee, Seung Jin,Lee, Kyung Suk,Jung, Young Ho,Jee, Hye Mi,Kwon, Tae Hee,Han, Man Yong,Sheen, Youn Ho The Korean Pediatric Society 2016 Clinical and Experimental Pediatrics (CEP) Vol.59 No.12

Purpose: Chest radiography is often performed on patients hospitalized with typical clinical manifestations of bronchiolitis. We aimed to determine the proportion of subjects with pathologic chest radiographic findings and the clinical predictors associated with pathologic chest radiographic findings in young children admitted with the typical presentation of bronchiolitis. Methods: We obtained the following data at admission: sex, age, neonatal history, past history of hospitalization for respiratory illnesses, heart rate, respiratory rate, the presence of fever, total duration of fever, oxygen saturation, laboratory parameters (i.e., complete blood cell count, high-sensitivity C-reactive protein [hs-CRP], etc.), and chest radiography. Results: The study comprised 279 young children. Of these, 26 had a chest radiograph revealing opacity (n=24) or atelectasis (n=2). Multivariate logistic regression analysis showed that after adjustment for confounding factors, the clinical predictors associated with pathologic chest radiographic findings in young children admitted with bronchiolitis were elevated hs-CRP level (>0.3 mg/dL) and past history of hospitalization for respiratory illnesses (all P<0.05). Conclusion: The current study suggests that chest radiographs in young children with typical clinical manifestations of bronchiolitis have limited value. Nonetheless, young children with clinical factors such as high hs-CRP levels at admission or past history of hospitalization for respiratory illnesses may be more likely to have pathologic chest radiographic findings.