항암제 처리한 백혈병 세포주에서의 Apoptosis 발현

김삼용,윤소현,김현수,김종숙,윤환중,김진경,조덕연 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

The bcl-2 proto-oncogene encodes a 26 kD protein that promotes cell survival by blocking apoptosis. The bax protein is a member of the bcl-2 familly, now known to form heterodimers with the bcl-2 protein. The ratio of bax to bcl-2 is be critical in determining the fate of the cell in response to stimuli that can induce apoptosis. Extract of Pulsatilla Koreana (SB-31) showed promising antitumor activity in vitro with Topo I inhibitory action. In the present study, the relationship between apoptosis and the apoptosis related proteins, bcl-2 and bax were investigated in human leukemic cell lines HL-60, U-937 and CEM-CM3. All anticancer drugs(adriamycin, etoposide, camptothecin, SB-31) induced extensive apoptosis in HL-60, U-937 cells and CEM-CM3 cells. The expression of bcl-2 and bax protein were determined in cell lines by western blotting before and after incubation with anticancer drugs at different time points. 1) In HL-60 or U-937 cell lines, down regulation of bcl-2 and up-regulation of bax were found after incubation with ADR, VP-16 or camptothecin. 2) In HL-60 or U-937 cell lines, no significant change in bcl-2 or bax protein expression resulted after incubation with SB-31. 3) In CEM-CM3 cells, virtually no change was noted in bcl-2/bax expression after incubation with ADR, VP-16, camptothecin or SB-31. It is suggested that different leukemic cell lines use different pathways of apoptosis activation and a given cell may utilize different pathways of apoptosis activation in response to different cytotoxic agents.

한 대학한방병원에 입원한 뇌졸중환자의 입원경로를 중심으로 한 기술역학적 고찰

김송,박종,김기순,이철갑,류소연,정영 조선대학교 부설 의학연구소 1998 The Medical Journal of Chosun University Vol.23 No.2

To find the characteristics of stroke patients and the relation between the pathway of admission and characteristics, of the patients including general characteristics, progression of disease and subjective complaints, an analysis was made from the medical record of 291 inpatients due to stroke admitted to a oriental medicine university hospital in 1997. 1. Diagnosis categories of analyzed Patients were 50 cerebral hemorrhage and cerebral infarction. 2. Sex distribution of patients showed 125 male and 165 female and age distribution showed peak 33.7% at 60-69 year old group, 26.5% at 70-79 year old group in descending order(p<05). 3. Thirty four percent of stroke patients had family history of stroke and male showed significantly higher rate of family history of than female(p<05). 4. The type of physical constitution was classified as Tea-Eum-In(太陰人) 43,6%, So-Eum-In(少陰人) 29.9%, So-Yang-In(少陽人) 26.1%, Tea-Yang-In(太陽人) 0.3% in descending order of frequency. 5. Hypertension was the preceding condition for 36.4% of cerebral hemorrhage and 42.7% of cerebral infarction. 6. Sixty nine point four percent of the patients of the patients suffered from the stroke for the first time and 25.4% for the recurred stroke. Major subjective complaints of the patients were motor disturbance, speech difficulty and headache in descending order. 7. As the institution for medical care to this admission, 138 person(47.4%) used western type hospital or clinics, 137 persons(47.1%) passed oriental medicine clinics or hospitals, 6 persons(2.1%) used both type of western medicine and oriental medicine and 10 person(3.4%) used pharmacy folk medicine. 8. As pathway of medical care for cerebral hemorrhage 71.2% of patients used western type hospital or clinic and 26.9% used oriental type of care where as for cerebral infarction 42.3% used western type and 51.5% used oriental type. 9. While admitted to this hospital within one day after occurrence of the disease used oriental type by 87.5% and western type by 14.3%, patient admitted in one week after occurrence of the disease used western type by 66.9% and oriental type by 27.1%. The above results showed that the pathway of medical care for the stroke patients was complicated and make many problems due to delay in the arrival to medical care. So effective health care delivery system for the stroke patients must be made for more efficient medical care.

一部 美容業 從事者들의 織責別 腰痛 有病率과 關聯要因

김춘일,박종,류소연,김석산,김기순,하상호,변주남 조선대학교 부설 의학연구소 1999 The Medical Journal of Chosun University Vol.24 No.1

To find the prevalence and related factors of low back pain experienced by some beauticians. a survey was made toward 252 beauticians in Kwangju City from March 1st to April 1st, 1998. The results were as follows; 1. The prevalence rate of low back pain among the respondents for the last week was 59.5%. 2. The aid group and staff group with low back Pain had significantly longer duration of work than those without low back pain. Managers who had bad posture with twisted or too much extended back showed a significantly higher prevalence of low back pain than those with good posture during work(P<05). 3. Aid group with low back pain showed significantly higher points at total stress score, depression score, general health score and vitality score than those without low back Pain. The staff group with low back pain showed significantly higher points for total stress score, sleep disorder and anxiety score, depression score, general well-being and vitality score than those without low back pain. Managers with low back Pain showed significantly higher points of stress at social performance and self confidence (P<05) than those without low back pain. The above results suggest that low back pain is related to different jobs and work environments for beauticians. So effective measures and further studies against risk factors must be made considering their jobs. To prevent or reduce low back pain, appropriate management of stress for beauticians is necessary.

국내 HIV 감염자에 있어서 Highly Active Antiretroviral Therapy (HAART)의 치료효과 및 영향분석

김명수,신소연,박윤선,김연아,구남수,김준형,김영근,최준용,송영구,김준명 대한감염학회 2007 감염과 화학요법 Vol.39 No.3

목적 : 전 세계적으로 에이즈 감염자가 4,000만명을 넘고 우리나라에서도 최근 하루에 2.1명의 신규 감염자가 발생하며 2006년까지 누적 감염자가 4,500명을 넘고 있다. 여러 가지 항레트로바이러스제가 개발된 이후 우리나라에서는 1997년부터 HIV 감염자에 대한 3제 병합요법이 시작되었으나 그 효과에 대한 우리나라의 연구는 미미한 실정으로 이에 대해 알아보고자 하였다. 재료 및 방법 : 연세대학교 의과대학 세브란스병원에서 2005년 12월까지 진료한 HIV 감염자 중 1997년 10월이후 항레트로바이러스 치료제를 투여 받은 환자 가운데 이전의 항레트로바이러스에 대한 치료력이 없으면서, 1년 이상 HIV-RNA 및 CD4+ T 림프구 수의 정기적인 측정인 가능한 141명의 환자를 대상으로 후향적 의무기록 분석을 통해 임상적인 특징과 6개월과 12개월째 HAART에 대한 치료효과를 분석하고 이와 관련된 요인을 찾고자 하였다. 결과 : 세브란스병원에서 2005년 12월까지 451명이 에이즈로 진료를 받았고, 1997년 이후 HAART를 시행받으면서 1년 이상 HIV RNA 및 CD4+ T 림프구수의 정기적인 측정이 가능한 환자는 141명으로 127명(90.1%)이 남자였다. 연령별 분포를 보면 30대가 50명(35.5%), 40대가 50명(35.5%)로 가장 많았다. HAART 시작 당시에 28명(19.9%)이 에이즈 정의질환을 동반하고 있었고, 74명(52.5%)은 에이즈 정의질환은 아니지만 기회감염을 동반하고 있었다. 121명(85.8%)의 환자에게 단백분해효소억제제를 기반으로 하는 처방이 이루어졌고, 20명(14.2%)의 환자에게는 비뉴클레오시드 역전사억제제를 기반으로 하는 처방이 이루어졌다. 치료 시작 당시 CD4+ T 림프구수는 평균 207/mm³(IQR 73-299)였고, 평균 HIV RNA는 70,800 copies/mL (4.85 log copies/mL)였다. HAART 시행 12개월 후에 평균 CD4+ T 림프구는 143 cells/mm³증가하였고, 96명(68.1%)에서 100 cells/mm³이상의 증가를 나타냈다. 치료반응에 따라 두 군으로 나누어 분석한 결과 12개월 후 100 cells/mm³이상 증가한 군이 초기 CD4+ T 림프구수가 적었다(P=0.01). HIV RNA 바이러스는 치료 당시보다 6개월후에 1.76 log copies/mL만큼 감소하였고, 12개월 후에는 1.88 log copies/mL만큼 감소한 결과를 보였다. 그리고, HAART 시행 후 HIV RNA는 6개월째 103명(73%)이 400 copies/mL 미만의 결과를 보였고, 12개월 후 90명(63.8%)에서 50 copies/mL 미만으로 측정되지 않는 결과를 나타냈다. HIV RNA를 치료반응에 따라 두 군으로 나누어 비교한 결과 6개월째는 두 군간의 차이가 없었으며, 12개월 후에는 50 copies/mL 미만인 군이 나이가 더 많은것으로 나타났다(P=0.009). 대상 환자 141명 가운데 초기처방을 중간에 변경한 경우가 57명(40.4%)이었고, 처방 변경의 주된 이유는 약제 부작용과 관련된 것이었다. 결론 : 국내에서 에이즈 감염자가 지속적으로 증가하고 있는 상황에서 HAART의 치료효과를 분석한 결과 기존의 치료 결과와 유사한 성적을 보였다. 앞으로 대규모의 코호트 연구를 통해 국내 에이즈 환자들의 특성 규명과 이에 알맞은 치료에 대한 연구가 더 필요하겠다. Background : Recently, 2.1 persons per day are reported to be HIV infected and totally more than 4,500 HIV infected persons have been officially reported until 2006 in Korea. We started highly active antiretroviral therapy (HAART) for HIV patients from 1997. Data on the efficacy of antiretroviral therapy are limited in Korea. Materials and Methods : HIV infected patients treated with HAART for more than 1 year were included. We studied the therapeutic response of antiretroviral therapy in patients with AIDS and without previous antiretroviral therapy who were treated until Dec 2005 in Severance hospital. Results : More than 450 were diagnosed as HIV infected in Severance hospital. Among these HIV infected patients, 141 patients including 14 females were included. 121 of the 141 patients (85.8%) were treated with PI based regimen, and 20(14.2%) were treated with NNRTI based regimen. The initial mean CD4 T cell count was 207/mm³ (IQR 73-299) and the mean HIV RNA level was 70,800 copies/mL. After 6 months of HAART, 103 patients (73%) had fewer than 400 copies of HIV RNA copies/mL. After 12 months of HAART, 90 patients (63.8%) had fewer than 50 copies of HIV RNA copies/mL. The mean increase in the CD4 T cell count from baseline to 12 months was 143 cells/mm³. After 12 months, 96 patients (68.1%) had increased more than 100 cells/mm³. Conclusion : This report the therapeutic response of HAART in Korea which the prevalence of HIV infection is now increasing. Overall, the outcomes are similar to those in other developed countries. Further large scale prospective cohort study is needed.

Depth Jump 시 하지 관절 상해에 관한 운동역학적 분석

소재무,김윤지,이종희,서진희,정연옥,김광기 한국운동역학회 2005 한국운동역학회지 Vol.15 No.1

J. M. SO, Y. J. KIM, J. H. LEE, H. J. SEO, Y. O. CHUNG ? K. K. KIM. The analysis of lower extremities injury on depth jump. Korean Journal of Sport Biomechanics, Vol. 15, No. 1, pp. 127-142, 2005. The purpose of this study was to analysis biomechanics of the lower extremities injury the heights(40cm, 60cm, 80cm) of jump box as performed depth jump motion by 6 females aerobic athletes and 6 non-experience females students. The event of depth jump were set to be drop, landing and jump. The depth jump motions on the force plate were filmed using a digital video cameras, and data were collected through the cinematography and force plate. On the basis of the results analyzed, the conclusions were drawn as follows: 1. The landing time of skill group was shorter than unskill group at 40cm, 60cm drop height during drop-landing-jump phase especially. The landing time of 60cm drop height was significant between two group(p<.05). 2. The peak GRF of sagittal and frontal direction following drop height improve was variety pattern and the peak vertical force of 40cm drop height was significantly(p<.05). 3. The magnitude of peak passive force was not increase to change the drop height. 4. The peak passive forces was significant at 40cm drop height between two groups(p<.05)

Evaluating the effects of honey bee (Apis mellifera L.) venom on the expression of insulin sensitivity and inflammation-related genes in co-culture of adipocytes and macrophages

Kim Hee‐Yeon,Jo Min Jeong,Nam So Yung,Kim Kwang Min,Choi Moon Bo,Lee Yong‐Ho 한국곤충학회 2020 Entomological Research Vol.50 No.5

Obese adipose tissue is characterized by adipocyte hypertrophy and a massive macrophage infiltration. The interaction between macrophages with mature adipocytes releases pro-inflammatory cytokines. This chronic inflammatory state can contribute to obesity-related complications, such as insulin resistance, type 2 diabetes and cardiovascular disease. Therefore, we can attempt to prevent and treat obesity-related diseases by inhibiting macrophage infiltration and blocking their interaction with adipocytes. Honey bee (Apis mellifera) venom (BV) has been reported to have anti-inflammatory effects. Although BV is used to treat chronic inflammatory diseases, few studies have addressed its use in obesity-associated inflammation. This study examines the inhibitory effects of BV on lipid accumulation in differentiating preadipocytes, inflammation, and insulin resistance in macrophages and adipocyte-macrophage co-culture system. We treated 3 T3-L1 preadipocytes with BV during differentiation. We later measured lipid accumulation and gene expression of master adipogenic transcription factors. After RAW264.7 and 3 T3-L1 cells were pretreated with BV, RAW264.7 cells were activated with LPS or co-cultured with pretreated 3 T3-L1 cells. Gene expression of pro-inflammatory cytokines and insulin sensitizing genes was measured in these cells. BV inhibited lipid accumulation and C/EBPα and PPARγ gene expression during intermediate and late 3 T3-L1 cell differentiation. BV also suppressed gene expression of pro-inflammatory cytokines (COX-2, iNOS, MCP-1, TNF-α, IL-1β and IL-6) in LPS-stimulated macrophages, and in co-culture of 3 T3-L1 adipocytes and RAW264.7 macrophages. However, adiponectin and GLUT-4 expression were both significantly increased by BV in co-culture. These findings demonstrate that BV attenuates adipocyte hypertrophy and improves obesity-related inflammation and insulin resistance in obese adipose tissue.

항암제 처리한 백혈병 세포주에서의 Apoptosis 발현

김삼용,윤소현,김현수,김종숙,윤환중,김진경,조덕연 충남대학교 암연구소 1998 癌共同硏究所 硏究誌 Vol.2 No.1

The bcl-2 proto-oncogene encodes a 26 kD protein that promotes cell survival by blocking apoptosis. The bax protein is a member of the bcl-2 familly, now known to form heterodimers with the bcl-2 protein. The ratio of bax to bcl-2 is be critical in determining the fate of the cell in response to stimuli that can induce apoptosis. Extract of Pulsatilla Koreana (SB-31) showed promising antitumor activity in vitro with Topo I inhibitory action. In the present study, the relationship between apoptosis and the apoptosis related proteins, bcl-2 and bax were investigated in human leukemic cell lines HL-60, U-937 and CEM-CM3. All anticancer drugs(adriamycin, etoposide, camptothecin, SB-31) induced extensive apoptosis in HL-60, U-937 cells and CEM-CM3 cells. The expression of bcl-2 and bax protein were determined in cell lines by western blotting before and after incubation with anticancer drugs at different time points. 1) In HL-60 or U-937 cell lines, down regulation of bcl-2 and up-regulation of bax were found after incubation with ADR, VP-16 or camptothecin. 2) In HL-60 or U-937 cell lines, no significant change in bcl-2 or bax protein expression resulted after incubation with SB-31. 3) In CEM-CM3 cells, virtually no change was noted in bcl-2/bax expression after incubation with ADR, VP-16, camptothecin or SB-31. It is suggested that different leukemic cell lines use different pathways of apoptosis activation and a given cell may utilize different pathways of apoptosis activation in response to different cytotoxic agents.

Clostridium difficile에 의한 설사의 예후인자

김준형,김희정,구남수,김영근,최준용,신소연,박윤선,김연아,김명수,정수진,최희경,송영구,이경원,김준명 대한감염학회 2007 감염과 화학요법 Vol.39 No.2

배경 : Clostridium difficile에 의한 설사(Clostridium difficile associated diarrhea; CDAD)는 임상 경과가 다양하다. 최근에 유럽과 북미에서 C. difficile 감염의 발생률이 증가하고 고전적 치료에 잘 반응하지 않으며 이환률이 증가하였고, 이러한 원인이 새로운 균주의 탄생에 기인한다고 보고되었다. CDAD의 예후에 영향을 미치는 세균성 요인과 숙주 요인을 확인하기 위해 본 연구를 진행하였다. 재료 및 방법 : 2002년 8월부터 2003년 12월까지 CDAD가 진단된 20세 이상인 환자들을 대상으로 후향적 Cohort 연구를 하였다. 세균성 요인을 확인하기 위해 cdt A, cdtB, tcd A, tcd A rep 그리고 tcd B 유전자(binary toxin)를 확인하였다. 설사가 치료 시작 후 11일 이상 지속되거나, 2달 이내에 재발하거나, 수술 혹은 다른 시술이 필요한 경우, 사망한 경우 예후가 좋지 않은 것으로 정의하였다. 결과 : 총 115예에서 toxin을 생성하는 C. difficile가 동정되었으며, Toxin A와 toxin B 모두 양성인 균이 91예, toxin B만 양성인 균이 24예였다. Toxin A 생성 여부가 예후에 영향을 미치지 않았지만, 제산제를 사용한 환자에서 그렇지 않은 환자에 비해 toxin B만 양성인 균이 많이 동정되었다(P<0.05). 예후가 좋지 않은 경우는 39예(33.9%)였고 76예(66.1%)에서 예후는 양호하였다. 단변량 분석에서 70세 이상의 고령, 남성, 증상 발현 후 사용한 항생제의 개수 사용, 증상 발현 후 carbapenem, aminoglycoside, glycopeptide 사용, 당뇨 및 뇌졸중 병력이 있는 경우, 그리고 높은 Charlson index가 불량한 예후 인자로 확인되었다. 그러나 독립적인 예후 인자를 조사했을 때에는70세 이상의 고령(odds ratio=3.378, P=0.009), 증상 발현후 carbapenem 사용(odds ratio 7.210, P<0.001)이 예후에 중요한 영향을 미치는 요소로 확인되었다. 결론 : 70세 이상의 고령과 증상 발현 후 carbapenem 사용이 CDAD 독립적인 불량한 예후인자이다. Background : Clostridium difficile associated diarrhea (CDAD) has a wide range of clinical manifestations. The prognostic factors of CDAD are not fully understood. Materialsand Methods : A retrospective cohort study of 115 patients with CDAD from Aug. 2002 to Dec. 2003 was conducted to evaluate prognostic factors of CDAD. Bacteriologic factors were determined by detecting the binary toxin gene, tcd A, tcd A rep and tcd B gene. Poor prognosis was defined as diarrhea more than 10 days even with classic treatment, recurrence, death, and moribund discharge. Results : Approximately 79% of isolated strains were toxin A+/B+ strains and 21% were toxin A-/B+ strains. There was no difference in prognosis between toxin A+ and toxin A- strains. 39 (33.9%) cases showed poor prognosis and 76 (66.1%) cases showed good prognosis. Univariate analyses revealed that the poor prognostic factors were old age over 70 years old, male, the number of antibiotics used after onset of symptom, the administration of carbapenems, aminoglycosides, glycopeptides after onset of symptom, history of DM and stroke, and high Charlson comorbidity index. Multiple logistic regression analysis identified old age over 70 years old (odds ratio=3.378, P=0.009) and the administration of carbapenems after onset of symptom (odds ratio 7.210, P<0.001) as the independent poor prognostic factors. Conclusion : Old age over 70 and the administration of carbapenems after onset of symptom were the poor prognostic factors for CDAD caused by none-binary toxin producing strains.

입원 중인 베트남 참전 군인의 삶의 만족도와 관련된 요인

김애리,박종,김율,노희송,박광희,김은숙,김형철,홍강식,류소연,강명근,김기순 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.2

Background and Objectives: The aim of the study was to identify the factors associated with life satisfaction of hospitalized Vietnam veterans. Material and Methods: The data were collected from 200 hospitalized Vietnam veterans of 5 veterans hospitals in Korea from September 13 to 23, 2004. Information of general characteristics, health behavior, disease history, family function, depression and life satisfaction were collected by structured questionnaire. Results: The mean of life satisfaction was 21.48(±6.03), and the mean of depression was 53.10(±9.64). The prevalence of depression was mild depression 24.0%, moderate depression 43.5% and severe depression 25.0%. Depression was associated with life satisfaction and depression was higher, life satisfaction was lower. Family function and age were positively associated with life satisfaction, Conclusion: Life satisfaction was affected by depression and family function in hospitalized Vietnam veterans. Therefore, it was thought that psychosocial approaches such as improve depression and family function in care of hospitalized Vietnam veterans is required.

정보 제공을 통한 건물에너지 절약 행동 유발에 관한 실험

김성수(Kim Sung-Su),김소연(Kim So-Yeon),김용훈(Kim Yong-Hun),임종연(Lim Jongyeon) 한국태양에너지학회 2020 한국태양에너지학회 논문집 Vol.40 No.6

As a measure to reduce energy consumption in buildings, the high performance of the outer skin and the high efficiency of the facility system are attracting attention to induce changes in the behavior of the inmates. A methodology in the field of behavioral science, so-called nudge, is being considered as an energy-saving measure. However, most of the existing empirical experiments are aimed at housing, which can be attributed to the fact that individual energy conservation acts as an economic incentive, making it easier to achieve its effect. This study reports the results of empirical experiments on energy saving in public spaces by nuts, which, so far, includes few examples. The experiment was conducted on 33 people in a university laboratory. The existing energy consumption patterns were measured for one month; only a few workers were regularly informed of the energy usage for two months, and the resulting changes in the energy consumption patterns were measured. The provision of energy consumption information has shown to increase the probability of energy saving, and it is particularly effective in promoting the energy-saving behavior of those willing to save energy.