벤조디아제핀 수용체 영상용 양전자 방출 핵종 표지 플루마제닐 유도체 [F-18](3-(2-Fluoro)flumazenil의 합성과 생체 내 분포

장영수,이숙자,강삼식,홍성현,이명철,이동수,정준기,정재민,조정혁 대한핵의학회 1999 핵의학 분자영상 Vol.33 No.6

Purpose: Radiotracers that bind to the central benzodiazepine receptor are useful for the investigation of various neurological and psychiatric diseases. [C-11]Flumazenil, a benzodiazepine antagonist, is the most widely used radioligand for central benzodiazepine receptor imaging by PET. We synthesized 3-(2-[F- 18]fluoro)flumazenil, a new fluorine-18 (t1/2=110 min) labeled analogue of benzodiazepine receptor imaging agent, and evaluated in vivo for biodistribution in mice. Materials and Methods: Flumazenil (Ro 15-1788) was synthesized by a modification of the reported method. Precursor of 3-(2-[F-18]fluoro)flumazenil, the tosylated flumazenil derivative was prepared by the tosylation of the ethyl ester by ditosylethane. [F-18] labeling of tosyl substitued flumazenil precursor was performed by adding F-18 ion at 85℃ in the hot cell for 20 min. The reaction mixture was trapped by C18 cartridge, washed with 10% ethanol, and eluted by 40% ethanol. Bidistribution in mice was determined after intravenous injection Results: The total chemical yield of tosylated flumazenil derivative was ∼40%. The efficiency of labeling 3-(2-[F-18]fluoro)flumazenil was 66% with a total synthesis time of 50 min. Brain uptakes of 3-(2-[F-18]fluoro)flumazenil at 10, 30, 60 min after injection, were 2.5±0.37, 2.2±0.26, 2.1±0.11 and blood activities were 3.7±0.43, 3.3±0.07, 3.3±0.09%ID/g, respectively. Conclusion: We synthesized a tosylated flumazenil derivative which was successfully labeled with no-carrier-added F-18 by nucleophilic substitution.

폐경전 여성에서 에스트로겐 수용체 유전자 다형성과 골초음파 지표의 상관관계에 대한 연구

김정순,정동진,강혜숙,강세훈,김경민,홍세인,조동혁,강호철,정민영 全南大醫大 2004 전남의대학술지 Vol.40 No.1

온풍 조건에서 수분 탈락 정도에 따른 피부 분류 및 개선 방안에 대한 연구

권오선 ( Oh Sun Kwon ),강현종 ( Hyun Jong Kang ),한승민 ( Seung Min Han ),윤지선 ( Ji S Eon Yoon ),조웅희 ( Woong Hee Cho ),오주영 ( Joo Young Oh ),임준만 ( Jun Man Lim ),송영숙 ( Young Sook Song ),박선규 ( Sun Gyoo Park ) 대한화장품학회 2020 대한화장품학회지 Vol.46 No.2

피부를 탄력있고 부드럽게 하는 역할은 각질층에 존재하는 수분량에 의해 좌우된다. 피부 수분량은 냉온풍, 건조환경 등 다양한 환경 변화에 의해 영향을 받음이 알려져 있으나, 개인 피부 차이에 따른 피부수분량 변화와 회복 정도에 대해서는 많은 연구가 이루어 지지 않은 실정이다. 본 연구에서는 온풍 조건하에서 피시험자들의 피부 수분 탈락 및 회복 정도를 비교 평가하여 새로운 피부 타입을 제시하고, 온풍 조건에서 저하되는 피부 수분량을 개선 시켜주는 효능 물질을 개발하고자 하였다. 온풍 환경 조성을 위해, 건강한 피험자(남: 10 명, 여: 39 명, 25 세 - 63 세)의 전완부에 온풍(30 cm, 40 ℃, 6 m/s)을 30 min 간 피부에 노출시켜, 피부 수분량의 변화를 평가하였다. 26명(남: 4 명, 여: 22 명, 평균 연령: 42.7 ± 9.4)이 온풍 노출전에 비하여 온풍 노출 후 수분량이 유의하게 감소하며, 노출 후 30 min이 지나도 회복이 되지 않았다. 온풍노출 후 수분량이 떨어지는 피험자(여: 10 명)를 대상으로 보수력이 높은 크림을 3 주간 전완부에 적용한 이후 동일 온풍 조건하에서 피부 수분량을 측정한 결과, 노출 30 min 후 피부 수분량이 온풍 노출 전 수준으로 회복됨을 확인하였다. 본 연구를 통하여 피부는 건조 조건에서 쉽게 수분을 잃어버리는 피부(탈수형 피부)가 존재하는 것을 확인하였다. 이는 앞으로 화장품 개발을 보습 기능뿐만 아니라, 이러한 환경변화에 따른 피부수분이 쉽게 빠져나가는 피부(탈수형 피부)의 특성에 맞는 제품의 효능 개발이 필요함을 보여준다. Elasticity and softness of the skin depend on the level of moisture present in the stratum corneum, which is known to be affected by various environmental changes, such as cold and hot winds and dry environments. However, not many studies have been conducted on changes in skin moisture and the degree of recovery due to individual skin differences. In the present study, we aimed to investigate the effect of warm air heating on skin hydration levels and develop moisturizing formulas to improve lowered skin hydration levels. In order to deliver warm air heating condition, heating dryer (40 ℃, 6 m/s, 30 cm apart from forearm) was applied into inner forearm of healthy subjects (male: 10, female: 39, age: 25 - 63) Among 49 subjects, 26 subjects showed significantly lowered skin hydration levels until 30 min after warm air heating exposure (lowered group). In addition, moisturizing cream with high water holding capacity was applied to forearm of 10 subjects in lowered group for 3 weeks and skin hydration levels after warm air heating were significantly improved at the levels of before application of warm air heating. From this study, we found out that there is a skin type that skin hydration levels are significantly decreased under warm air heating condition (dehydrated skin) and this dehydrated skin can be improved by moisturizing formulas with high water holding capacity.

로스팅 서리태를 이용한 음료 제조 및 품질 평가

정수옥,김혜연,한정순,김민주,강미숙,김애정,Jeong, Soo-ok,Kim, Hae-yean,Han, Jung-soon,Kim, Min-ju,Kang, Mi-sook,Kim, Ae-Jung 한국식품영양학회 2016 韓國食品營養學會誌 Vol.29 No.4

This study was performed to develop and evaluate beverage prepared with optimally roasted seoritae to maximize the isoflavone content and antioxidant activities of the beverage. Isoflavone content was maximized at the roasting temperature of $110^{\circ}C$ for 20 min. Both DPPH radical scavenging activity and ABTS radical scavenging activity along with total polyphenol content were highest when seoritae was roasted at $110^{\circ}C$ for 20 min. Western blotting was used to determine the level of nuclear factor of activated T-cells 1(NFATc1) involved in controlling osteoclast differentiation. The results showed that NFATc1 had a concentration-dependent inhibitory effect when the RoS110 (roasted seoritae at $110^{\circ}C$ for 20 min) samples were processed at varying concentrations (10, 50, and $100{\mu}g/mL$). Tea samples were prepared from optimally roasted seoritae by varying brewing times (5~90 min) at $65^{\circ}C$, and tea brewed for 60 min had the highest preference with $65^{\circ}C$ as the preferred temperature for drinking.

제주마의 체형에 관한 연구

강민수,정창조,강태숙,박희석,김동후,정재준 제주대학교 농과대학 제주도축산문제연구소 1986 畜産論叢 Vol.1 No.1

제주마의 모색빈도에 관한 연구

강민수,정창조,강태숙,정재준 제주대학교 농과대학 제주도축산문제연구소 1988 畜産論叢 Vol.3 No.1

The coat color records of 210 Cheju Island pony were analyzed to examine if the frequency is independent of sex. In Chelu Island pony the frequencies of chestnut color were 35.7 and 39.7 percent respectively in the male and female, and those of bay color were 21.4 and 31.6 percent respectively. Other colors(black, fallow, piebald)tended to be more frequent in the female than in the male. The Chi-square tests for the sex x coat color table showed that the sex differences were significant.

대두요구르트제조를 위한 두유에서의 젖산균의 산생성과 침전에 관한 연구

강성태,정종민,김제중,정병옥,이흠숙,정석진 서울産業大學校 1999 논문집 Vol.49 No.2

전지두유에 시유를 각각 다른 비율로 혼합하여 제조하여 L. bulgaricus를 접종한 후 산생성에 미치는 효과를 조사하였다. 우유의 첨가량을 늘릴수록 24시간 후의 적정산소가 증가하여 두유에 유유를 혼합시키는 것이 L. bulgaricus에 의한 산생성에 유리함을 알 수 있었다. 전지두유를 100℃에서 30분, 60분, 90분간 가열하고 121℃에서 15분간 열처리하여 L. bulgaricus와 L. casei를 단독으로 접종하거나 1:1(v/v)로 혼합하여 접종하고 두유의 열처리가 산생성과 커드의 침전에 미치는 영향을 평가하였다. 두유의 열처리는 전반적으로 열처리되지 않은 두유보다 산생성을 촉진하였으며 산생성은 가열처리 정도에 비례하였다. 또한 모든 배양에서 121℃ 15분간의 열처리는 100℃ 90분의 열처리보다 낮은 산생성을 보여주었다. 또한 두유의 열처리는 제조된 두유요구르트의 커드의 침전을 지연시키는 효과가 있었다. Soy milk was added with different ratio of market milk. Acid production of L. bulgaricus in mixtures were investigated. Acid production by L. bulgaricus increased in proportion to concentration of milk added to soy milk. And the effects of various heat treatment of soy milk on acid production by L. bulgaricus and L. casei alone or combined were investigated. Sedimentation characteristics of soy yogurt prepared from heat treated soy milk were also evaluated. Heat treatment of soy milk stimulated acid production by all cultures. Acid production was generally proportional to the degree of heat treatment. However, Heat treatment of soy milk at 121℃ for 15min showed rather lower acid production than that at 100℃ for 90 min. Heat treatment of soy milk generally retarded sedimentation of curd in soy yogurt beverage.

말초신경손상에 의한 척수감각신경 세포의 감작

강석한,김찬,엄민숙,김진혁,김기순 한양대학교 의과대학 1997 한양의대 학술지 Vol.17 No.1

Peripheral nerve injuries have been known to result in a chronic neuropathic pain characterized by symptoms of spontaneous burning pain, hyperalgesia, and allodynia. Recently it has been reported that the flexor reflex was greatly enhanced immediately after peripheral nerve section in the experimental animals. The persent study was underaken to investigte acute effects of the peripheral nerve injury on the sensitization of dorsal hom cells. After the sciatic nerve section, neither significant change was observed in the wind-up of WDR cells, nor wind-up was produced in the WDR cell that had not shown wind-up before the afferent nerve section. The A-fiber responses of WDR cells were not altered after section of the sciatic nerve. However, the C-fiber responses and after-discharge of the dorsal horn cell to imput signals started to increase in 2 hours after the nerve section. In 70% of experimental animals, the nerve section-induced increments in the WDR cell activities were abolished after topical application of 2% lidocaine to the proximal end of cut sciatic nerve. After pretreatemnt of the experimental animals with a NMDA receptor blocker, MK-801, the C-fiber response and after-discharge of the dorsal horn cell well not changed by the sciatic nerve section. These findings suggest that peripheral injury induces the sensitization of dorsal horn cells of the spinal cord and that signals from the proximal stump is essential for maintenance of the sensitization, which was mediated by NMDA receptors.

감귤박, 다시마, 손바닥 선인장 분말을 함유한 식이의 급여가 고콜레스테롤 혈증 흰쥐의 체네 지질수준과 장내 콜레스테롤 흡수, 혈소판 응집성 및 간 조직에 미치는 영향

강민숙,강정숙 제주대학교 방사능이용연구소 2000 연구보고 Vol.14 No.-

This study was done to investigate the hypocholesterolemic effect of low dietary fiber in a high cholesterol diet in aspect of cholesterol absorption and excretion using Sprague Dawley rats. After feeding diets containing 0.5% cholesterol and 5% tangerine pulp, sea tangle or prickly pear cactus for four weeks, we measured the levels of plasma and liver cholesterol and triglyceride, fecal neutral sterols for cholesterol excretion and fecal radioactivity after feeding 14C-cholesterol for cholesterol absorption. We also examined platelet aggregation and histological change in liver tissues in associaion of hypercholesterolemia. The liver to body weight ratio was significantly (p3<0.01) lower in rats fed prickly pear cactus than in other groups. The levels of plasma total cholesterol, LDL-cholesterol and triglyceride were decreased significantly (p<0.01) in the prickly pear cactus group compared to the control, while there was no difference in the liver levels of total cholesterol and triglyceride among groups. Fecal corprostanol and cholesterol were significantly (p<0.01) higher in groups of control and prickly pear cactus compared to the other two groups. Radioisotope excretion after ingestion of 14C- cholesterol was higher in the control group than in tangerine pulp or sea tangle, with the highest excretlon in prickly pear cactus among groups. Radioisotope excretion was the most during the 2nd and 3rd days in all groups. Hematocrit and platelet aggregation were decreased in all fiber groups compared to the control, but not statistically different. Microscopic examination showed that cholesterol diet caused a fat accumulation in the liver and prickly pear cactus decreased the fat accumulation. Result indicates that prickly pear cactus has hypocholesterolemic effect by decreasing absorption and increasing excretion of cholesterol, thereby protective effect on fatty liver. Control group fed diet containing high cholesterol and low fiber seems to have a self control system in cholesterol absorption and excretion preventing hypercho1esterolemia.

77K와 상온에서 CO(NH₂)₂의 ¹⁴N Pulsed NQR 연구

강재필,박영민,전인,김성재,최숙자,박현진,송승기,서용문 명지대학교 자연과학연구소 1996 자연과학논문집 Vol.13 No.-

77K와 상온에서 CO(NH₂)₂분말 시료의 ??N Pulsed NQR실험을 하여 위와 아래 공명 진동수, 핵 사중극 결합상수, 비대칭 인자, 스핀-스핀 완화시간, 결정 격자 진동에서의 평균 비틀림 진동수, 진동자의 관성 모먼트 등을 구하였다. 공명 진동수의 온도 의존성은 전형적인 Bayer이론의 격자 진동에 의한 주파수 변화를 보였다. Bayer-Kushida이론의 고온근사식을 실험값에 적용하여 OK에서 공명 진동수 ν??와 ν??를 구했으며, 77K와 상온에서의 스핀-스핀 완화시간 T₂를 관측했다. 상온에서 T₂의 감소는 결정 내의 reorientation motion과 관련이 있을 것으로 보인다. Upper and lower resonance frequencies, nuclear quadrupole coupling constants, asymmetry parameters, spin-spin relaxation time, average torsional frequency in the crystal lattice motion and moment of inertia in the oscillator are found for CO(NH₂)₂powder sample by using ??N Pulsed NQR(Nuclear Quadrupole Resonance) technique at 77K and room temperature. Temperature dependence of resonance frequencies showed frequency variation in agreement with the typical Bayer's theory in lattice motion. Resonance frequencies ν?? and ν?? at OK were obtained by fitting the high temperature approximation of Bayer-Kushida theory to the experimental data and spin-spin relaxation time (T₂) was observed at 77K and room temperature. Decrease of T₂at room temperature was explained by the effect of reorientational motion in the crystal lattice.