Purge & Trap-GC를 이용한 의약품 필름코팅 정제 중 잔류용제에 관한 연구

장준식,이명자,소유섭,문춘선,이주헌,박희라,김진숙,강경모,이선옥,방성연,유미자,유문균,금오성,이병욱 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-

의약품은 약물을 생체에 적풋하기 위하여 유효성분의 효과가 언제나 일정하게 확보되고 사응에 편리하도록 만들어지는 것이므로 유효썽분 이외에 약효에 영향을 주지 않는 성분이 첨가되는 경운가 많다. 이 때 사용되는 용매들은 제피의 광택 및 건쪼시간의 단축 등을 위하여 휘발점이 낮을 용매들이 주로 사용되어진다. 본 연구는 의약품 필름코팅정제 중 잔류용매 4종(chlorofonr benzen, trichloro ethylen, 1,4-dioxane)에 대한 변형된 pirge & trap-GC 장치를 이용한 동시분석방법을 개발하였으며, 각 표준품의 RSD 값은 chloroform 3.03%, benzen 3.17%, trichloroethylen 3.69% and 1,4-dioxane 3.41%였다. 또한 시중 유통중인 의약품 50종에 대하여 잔류웅매 양을 측정하였으며, 검출되는 잔류용매는 한 건도 없었다. This study nras carried out to develope the analytical method for the mixture of chlorefonn, benzen, trichloroethylen and 1,4-dioxane simultaneously and determine the remainingorgauic solvents in coating tablets by Purge & Trap-GC. The results were as follouFs ; 1. Chloroform, benzen, trio:tloroethylen and 1,4-dioxane separated by tenax #5 trap by HP-624GC column by terrlperature programming. The peaks were separated completely at retentiontime of 6.88min for chloroform, 8.21min for benzen, 10.38miu for trichloroethylen and 11.95minfor 1,4-dioxane. 2. Standard RSD were individually chloroform 3.03%, benzen 3.17%, trichloroethylen 3.69%and 1,4-diorane 3.41%. 3. 60 samples were not detrcted chloroform, benzen, trichloroethylen and 1,4-dioxane.

세포질내 정자주입법(ICSI)에 있어서 정자흡입 및 난자내 주입방법에 관한 연구

이택후,김항진,송건호,김대근,전상식,박윤규,서태광,전병균,류은경,이은숙,문진수,김광철 경북대학교 의학연구소 2000 경북대학교병원의학연구소논문집 Vol.4 No.1

Study on Method of Sperm Aspiration and Injection into an Oocyte in Intracytoplasmic Sperm Injection(ICSI) Immobilization of spermatozoa prior to intracytoplasmic sperm iniection(ICSI) sometimes results in crooked tail and this makes it difficult to aspirate sperm into an injection pipette tail first. Head-first sperm aspiration into an injection pipette avoid this problem due to the bigger size of the sperm head. The effect of head or tail-first sperm injection into an oocyte on fertilization cleavage, percentage of grade I embryos and development to blastocyst stage in ICSI program has been studied. A single living immobilized spermatozoa from oligoasthenozoospermic patient was injected into an oocyte head-first or tail-first according to the treatment. Eighteen hours after microinjection, oocytes ware inspected for survival and fertilization Fertilized oocytes with two pronuclei were cultured in 30μl drop of mHTF supplemented with 10% heat-inactivated follicular fluid(FF) at 37℃. On day 2. embryo transfer was performed with cleaved embryos. The remaining 2-8 cell stage embryos were co-cultured with BRL cells in mHTF + 10% FF for 72 hours and the developmental stage was observed. The data were analyzed by Analysis of Variance. A total of 164 oocytes from 36 cycles were assigned to earth treatment and ICSI was performed(88 head-first, tail-first). The rates of normal fertilization were 81.8% and 76.3% for head-first and tail-first, respectively. Of the fertilized oocytes, the percentage of cleaved embryos and the percentage of grade 1 embryo among cleaved embryos were 88.9% and 68.8%, 93.1% and 74.1% for head-first and tail-first, respectively. Of the 2-8 cell embryos cultured, 44.4%(16/36) and 50.0%(10/20) for head first and tail first, respectively developed to blastocyst stage. There were no differences in fertilization, cleavage, rates of grade 1 embryos, and development to blastocyst stage. In conclusion, head-first or tail-first sperm injection into an oocyte in ICSI program does not affect fertilization and subsequent embryo development to blastocyst stage in vitro.

우리나라 무도의 현황과 과제에 관한 연구

이병익,김병천 대한무도학회 2000 대한무도학회지 Vol.2 No.1

The purpose of this study is to investigate the situation of the Korean martial arts based on their contemporary concepts and patterns and propose the tasks to be accomplished by dividing them into martial arts for elite and martial arts for all and analyzing them. The problems for Korean martial are as follows: First, Korean organizations of martial arts have not overcome the distorted history and the absence of concrete ideas. Korean martial arts were introduced from Japan during its control over Korea, and partially from China. However no organizations accept this historical background. The 'Koeanization' with denial of true history has produced the absurd reasoning on the history of martial arts. Most discussions on them have been dominated by narrow and blind obsession on tradition and scientification, resulting in commercialization. Second, unbalanced support and academic unreadiness are pointed out. Putting a priority on Martial arts as sports has resulted in emphasizing their athletic aspects lusing their original meaning. The government's unbalanced supports have prevented martial arts as sports for all from being developed. The government's policy only including such sports as judo, kendo, taekwondo, ssireum, and ushu for the licence of sports leaders is against the rule of fairness by neglecting the supervision at the national level. Third, inappropriate research methods on martial arts should be considered. Most researches on martial arts are composed of personal views and prejudice with few literature, which results in the lack of validity and reliability. The athletic-oriented curricula of undergraduate schools and the tradition -oriented research trends fail to present long-term perspectives and research methods, limiting the current status of martial arts on the past history and invalid research method. Recommendations for further study based on these problems are as follows. First, the concrete ideas and concepts on martial arts should be provided. Academic foundation with understanding the historic changes of oriental culture and the meaning of martial arts must be established. Second, integrated policy for promoting martial arts is indispensable. Establishment of centralized organizations administering their affiliates is strongly suggested. It will facilitate the popularization of martial arts, the integration of similar kinds of martial arts, and the systematization of knowledge. Third, policy for supporting the academization of martial arts are urgently needed. To realize this, support for seminars and academic associations should be done free from political considerations.

점도, 시편형태 그리고 접착의 유무가 광중합 복합레진의 선형중합수축의 측정에 미치는 영향

이인복,손호현,권혁춘,엄정문,조병훈 大韓齒科保存學會 2003 Restorative Dentistry & Endodontics Vol.28 No.6

Objectives. The aim of study was to investigate the effect of flow, specimen geometry and adhesion on the measurement of linear polymerization shrinkage of light cured composite resins using linear shrinkage measuring device. Methods. Four commercially available composites an anterior posterior hybrid composite Z100, a posterior packable composite P60 and two flowable composites, Filtek flow and Tetric flow were studied. The linear polymerization shrinkage of composites was determined using 'bonded disc method' and 'non bond ed' free shrinkage method at varying C factor in the range of 1~8 by changing specimen geometry. These measured linear shrinkage values were compared with free volumetric shrinkage values. The viscosity and flow of composites were determined and compared by measuring the dropping speed of metal rod under constant load. Results. In non bonded method, the linear shrinkage approximated one third of true volumetric shrink age by isotropic contraction. However, in bonded disc method, as the bonded surface increased the linear shrinkage increased up to volumetric shrinkage value by anisotropic contraction. The linear shrinkage value increased with increasing C factor and approximated true volumetric shrinkage and reached plateau at about C factor 5~6. The more flow the composite was, reduced linear shrinkage was measured by compensation radial flow.

K^+통로개방제 Pinacidil이 종양이식 생쥐에서 Tl-201의 체내분포에 미치는 영향

이재태,천경아,이상우,강도영,안병철,전수한,이규보,하정희 경북대학교 의학연구소 1999 경북대학교병원의학연구소논문집 Vol.3 No.1

Purpose: Thallium behaves similarly to potassium in vivo. Potassium channel opener (K-opener) opens ATP-sensitive K+ -channel located at cell membrane, resulting in potassium efflux from cytosol. We have previously reported that K-opener can alter biokinetics of T1-201 in cultured cells and in vivo. Malignant tumor cells have high Na-K ATPase activity due to increased metabolic activities and dedifferentiation, and differential delineation of malignant tumor can be possible with T1-201 imaging K-opener may affect tumoral uptake of T1-201 in vivo. To investigate the effects of pinacidil (one of the potent K-openers) on the localization of the tumor with T1-201 chloride, we evaluated the changes in biodistribution of T1-201 with pinacidil treatment in tumor-bearing mice. Materials and Methods: Balb/c mice received subcuta-neous implantation of murine breast cancer cells in the thigh and were used for biodistribution study 3 weeks later. 100㎍ of pinacidil dissolved in 200 □ DMSO/PBS solution was injected intravenously via tail vein at 10 min after 185 KBq (5 □Ci) T1-201 injection, Percentage organ uptake and whole body retention ratio of T1-201 were measured at various periods after injection, and values were compared between control and pinacidil-treated mice. Results: Pinacidil treatment resulted in mild decrease in blood levels of T1-201, but renal uptakes were markedly decreased at 30-min, 1- and 2-hour, compared to control group. Hepatic, intestinal and muscular uptake were not different. Absolute percentage uptake and tumor to blood ratios of T1-201 were lower in pinacidil treated mice than in the control group at all time points measured. Whole body retention ratio of T1-201 was lower in pinacidil treated mice (58±4%), than in the control group (67±3%) at 24 hours after with injection of 100 □g pinacidil. Conclusion: K-opener did not enhance, but rather decreased absolute tumoral uptake and tumor-to-blood ratios of T1-201 Decreased whole body retention ratio and renal uptake were observed with pinacidil treatment in tumor-bearing mice. (Korean J Nucl Med 2000;34:303-11)

鹽生植物 나문재의 生育特性과 利用可能性

이봉호,문윤호,정병춘,김관수,류수노 한국국제농업개발학회 2002 韓國國際農業開發學會誌 Vol.14 No.2

기능성식품 원료, 채소, 식물성 소금 재료 및 제염식물 등으로 개발이 유망시되는 염생식물인 나문재의 육지부 재배 가능성을 조사하고, 내륙지방에서의 작물화 가능성을 검토코자 전남 서해안의 염전 부근에 자생하는 나문재의 종자와 식물체를 공시하여 내륙지방인 전남 무안군 소재의 『목포시험장』포장에서 시험하였던 바 그 결과를 요약하면 다음과 같다. 가. 나문재는 식물체를 이식하여도 생육이 양호할 뿐만 아니라 2년 묵은 종 자에서도 발아가 잘되었으며 발아소요 기간은 9일 정도 였고, 나문재의 생육은 소금기가 부족한 내륙에서도 비교적 양호하였으며, 병충해나 기타 재해도 거의 없어 내륙에서의 재배화 가능성이 높은 것으로 인정되었다. 나. 나문재의 생육에 미치는 소금물의 공급 효과를 검토하기 위해 1주일 간격으로 바닷물 농도의 소금물을 공급한 결과, 초장은 소금물을 공급한 것이 공급하지 않은 것 보다 양호하였으나 줄기의 굵기와 분지발생 수 는 소금물 공급효과가 크지 않았다. 다. 나문재의 생체중과 건물중은 종자파종의 경우 7월 10일 현재 소금물 처리구는 각각 주당 33.75g, 2.62g인데 비해 무처리구는 각각 23.52g, 1.84g으로서 소금물 처리구가 무겁고, 생체중에 대한 건물중의 비율은 대체로 7.14∼11.58% 범위로서 수분함량은 92.86∼88.42%정도 되는 다육식물 이었다. 라. 결론적으로 나문재는 소금기가 많은 곳에서 잘 생육할 뿐만 아니라 소금 기가 적은 곳에서도 생육이 비교적 양호하며, 식물전체에 짠맛을 가지고 있으므로 식물성 소금, 기능성 식품, 채소, 그리고 간척지나 음식물쓰레기의 염분제거 식물로서의 이용 가능성 등에 대한 보다 면밀한 검토가 필요하다고 보여진다. The plant of halophyte, Suaeda asparagoides MIQ, which is mainly inhabit on tidal flat was investigated on their growth characteristics and cultural traits on inland condition, in order to get some informations on it's possible use for functional food, vegetables, medicinal herb, and salt removing plant from tideland, or food-waste. The plant, Suaeda asparagoides M_IQ., is well grown on inland condition. But the salt applicated plants were better than those of the non-treated plants in its vegetable growth. The plant height is about 78.5cm in salt application, compared to the 64.5cm in non-treated plot. But the stem diameter and the number of branches are not better in salt application compared to the non-treated ones. However, the fresh weight and dry weight of the plant in salt-application are better than those of the non-treated ones, which means the total biomass is more productive by the salt application. As a conclusion, the plant Suaeda asparagoides M_IQ, seems to be an available plant and need to more discussion for possible use mentioned in the above sectors, from the results of inland cultivation of which well grown and characteristics.

동결건조 장미의 화색과 형태적 특성

서춘순,박윤점,이일병,허북구 한국화훼연구회 2001 화훼연구 Vol.9 No.2

Rosa hybrida L. 'Sandra' was freeze dried and investigated the length fur drying, color change of petal, leaf and stem, and the change of flower diameter, 12 days were needed to dry flower completely by natural drying method, otherwise 2 days needed by freeze drying. There was significant difference between fresh flower and natural dried flower in terms of L, a, b value of petal color, otherwise there was no significant difference between fresh flower and freeze-dried flower. L, a and b value of leaf and stem of freeze-dried flower was different comparing with those of fresh flower, however the degree of difference was not higher than these of natural-dried flower, There was no significant difference between fresh flower and freeze-dried flower in terms of anthocyanin content in petal. The change of flower diameter of natural-dried flower and freeze-dried flower comparing with fresh flower was respectively 52.0% and 9.9%.

大麻 低마약형 신품종 "청삼"의 육성경위 및 주요특성

문윤호,이봉호,정병춘,김용웅,김길용 한국국제농업개발학회 2002 韓國國際農業開發學會誌 Vol.14 No.2

대마의 저 마약형 신품종을 육성하고자 대마초의 환각성분에 대한 국내의 문헌을 수집하여 고찰하여 저마약형 대마 신품종 "청삼(Cheungsam)"을 육성하였던 바 이것들을 요약하면 다음과 같다. 가. 목포시험장에서 1997년 네덜란드 CPRO에서 도입한 44품종의 저 마약형 유전자원중 IH3이 THC 함량이 낮고 개화일수도 한국재래종과 비슷하여 경장과 섬유수량도 다른 도입품종들에 비해 크고 많아 육종재료로 유망시 되었다. 나. 저 마약형인 IH3를 모본으로 하고 한국 재래종을 부본으로 교배한 1대잡종 품종을 육성하여 무안 등 4개 지역에서 지역적응시험을 실시한 결과 특성이 우수하고 저 마약형으로 분류되어 이를 "청삼"으로 명명하여 신품종으로 등록하였다. 다. "첨상" 품종은 섬유수량은 190kg/10a로 재래종의 177kg/10a에 비하여 7% 중수되었고, 환각성분인 THC(Δ-9-Tetrahydrocannabinol)함량이 0.34%로 재래종의 1.74%보다 적고 환각억제성분인 CBD(Cannabidiol)함량은 1.34%로 재래종의 0.57%보다 많아 저 마약형으로 분류할 수 있다. 라. "청삼" 종자를 IH3의 자웅이주종자를 이용하여 채종하면 종실수량이 103kg/10a이지만 자성종자 채종기술을 이용하여 채종하면 146kg/10a로 채종량이 42% 증가하였다. In order to develop a new variety of fiber hemp(Cannabis sativa L.) with non-drug type, the authors reviewed several literatures about marihuana or hemp, and bred a new variety with non-drug type. This variety was named as "Cheungsam" and resitered as a recommening variety in Korea. This paper introduce the breeding history and characteristics of the fiber hemp variety "Cheungsam" with non-drug type. Among the 44 introduced germplasms from CPRO, Netherland, only IH3 was suitable variety because of it's good traits including low THC content, similar flowering days to Korean local variety and good fiber yield. IH3 and Korean local variety were crossed to develop a new non-drug type hemp, and we developed a new variety named "Cheungsam". This variety have good plant characteristics and fiber yield with low THC content. The average fiber yield of "Cheungsam" was 190kg/10a in regional fiber yield trials, which was 7% higher than that of "Korean local variety". "Korean local variety" contains 1.74% of THC which is the psychoactive ingredient and 0.57% of CBD the anti-psychoactive ingredient, thus that cultivar was sorted to drug type hemp. But "Cheungsam" contains 0.34% of THC and 1.34% of CBD so that sorted as a non-drug type hemp. If farmers want to harvest more seed of "Cheungsam", using female seed of IH3 is better than that of the dioecious seed because of the high seed yield.

관절 내시경술에 의한 퇴행성 슬관절염의 치료

김동헌,장병춘,신규철,이재성 건국대학교 의과학연구소 1999 건국의과학학술지 Vol.9 No.-

The arthroscopic surgery is an increasingly accepted procedure for the treatment of symptomatic arthritic knee. Authors reviewed the operative results of arthroscopic surgery in 35 knee joints of 27 patients from 1993 to 1997. 1. The average age of patients was 64 years old and the primary diagnosis was degenerative osteoarthritis in all patients. 2. Preoperative symptomes and signs were pain(98%), tenderness(85%), swelling(85%), decreased range of motion(71%) and effusion(55%). 3. The operative procedures were irrigation and synovectomy(20%), abrasion arthroplasty(57%), partial menisectomy and abrasion arthroplasty(23%). 4. The symptoms and signs were improved postoperatively: according to Nine point scale, the good results were recorded at postoperative 6 months and follow-up 2 years in nearly all patients. 5. The complications were minimal. Authors concluded that arthroscopic surgery is considered as a reliable method in the treatment of degenerative osteoarthritis of the knee but not as a curable method, needing a long term observation.

버섯 중독 환자에서의 ^(99m)Tc-galactosyl human serum albumin (GSA) scintigraphy 소견 : 간초음파 소견과의 비교 Comparison with Liver Ultrasonography

정신영,이재태,배진호,천경아,안병철,강영모,정재민,이규보 대한핵의학회 2003 핵의학 분자영상 Vol.37 No.4

광대버섯과 같은 독버섯 중독시 간세포에 독성을 나타내어 간 기능의 이상을 유발할 수 있다. 저자들은 개나리광대버섯을 섭취한 3명의 환자에서 간 기능을 평가하기 위하여 간세포에 선택적으로 섭취되어 간기능을 평가하는데 사용되어지고 있는 99mTc-galactosyl human serum albumin (Tc-GSA)을 이용한 간 스캔을 시행하였고 간 초음파 검사와 비교하여 보았다. Tc-GSA 185MBq (3mg of GSA)을 정맥주사후 30분동안 동적 영상과 간과 심장 부위의 시간 방사능 곡선을 얻었다. 간기능의 정도를 평가하기 위하여 Tc-GSA의 간섭취 정도와 혈중 정체정도를 시각적인 평가와 간과 심장 부위의 시간 방사능 곡선을 이용한 반정량적인 평가를 시행하여 간섭취지수와 혈중제거지수를 구하였다. 시각적인 평가에서 2명의 환자에서 Tc-GSA의 경미한 정도의 간섭취 감소와 혈중 정체를 보였고 1명에서는 중등도의 간섭취 감소와 혈중 정체를 보였다. 반정량적인 평가에서는 2명의 환자에서 경미한 정도의 간섭취지수의 감소와 혈중제거지수의 증가를 보였고 1명에서 중등도의 간섭취지수의 감소와 혈중제거지수의 증가가 관찰되었다. 간초음파상에서는 1명의 환자는 지방침윤이 관찰되었으며 1명의 환자에서는 미약한 정도의 지방간이 관찰되어 급성 간기능 장애를 적절히 반영하지 못하였다. 본 증례를 통해 저자들은 간초음파 검사가 특이한 소견을 나타내지 못하여 간세포에 영향을 주는 독소나 약물 중독시에 간 영상화에는 Tc-GSA 간스캔이 유용하게 사용될 수 있을 것으로 추측할 수 있었다. ^(99m)Tc-galactosyl human serum albumin (Tc-GSA) is a radiopharmaceutical that binds to asialoglycoprotein receptors, which are specifically present in the hepatocyte membrane. Because these receptors are decreased in hepatic parenchymal damage, the degree of Tc-GSA accumulation in the liver correlates with findings of liver function test. Hepatic images were performed with Tc-GSA in patients with acute hepatic dysfunction by Amantia Subjunquillea poisoning, and compared with these of liver ultrasonography (USG). Tc-GSA (185 MBq, 3 mg of GSA) was injected intravenously, and dynamic images were recorded for 30minutes. Time-activity curves for the heart and liver were generated from regions of interest for the whole liver and precordium. Degree of hepatic uptake and clearance rate of Tc-GSA were generated by visual interpretation and semiquantitative analysis parameters (receptor index :LHL15 and index of blood clearance :HH15). Visual assessment of GSA scintigraphy revealed mildly decreased liver uptake in all of subjects. The mean LHL15 and HH15 were 0.886 and 0.621, graded as mild dysfunction in 2, and mild to moderate dysfunction in 1 subject. In contrast, liver USG showed no remarkable changes of hepatic parenchyme. Tc-GSA scintigraphy was considered as a useful imaging modality in the assessment of the hepatic dysfunction.