유제놀에 의한 즉시형 알레르기 반응의 억제

김상현,신태용,김형룡,이영미,이은희,신보경,김윤철,안년형,김형민 우석대학교 의약품개발연구소 1996 藥學硏究誌 Vol.1 No.-

The current study evaluates the capacity of eugenol to regulate immediate allergic reaction by control of histamine release. Administrations of eugenol (1 M/kg. i.p.) at 60 min before and 5.10 min after the compound 48/80 treatment (8 mg/kg.i.p.) were shown the mortality rates as 0.44.4. and 77.8%. respectively. A 60 min before administered group revealed a singificant inhibition of serum his-tamine release compared with those of 5 and 10 min after the compound 48/80 injection. Eugenol (6-48mM) was also showed a dose-dependent activity on the compound 48/80-induced histamine release from the highly purified population of Alcian Blue-positive peritoneal mast cells. These results indicate that in vitro treatment with exogenous eugenol inhibited the active response of mast cell populations and modulated its characteristics.

박의 RAPD 분석을 위한 PCR 최적 조건 연구

김보경,김성만,김용철,이충열,박현철,최인수 밀양대학교 농업기술개발연구소 2000 農業技術開發硏究所報 Vol.4 No.1

This study was conducted to identify the best combinations of factors(template DNA concentration, MgCl2 concentration, and amount of taq polymerase) for the optimization of PCR in gourd. 3×3×3 factorial experiment for template DNA concentration, MgCl2 concentration, and amount of taq polymerase was conducted. Another factorial experiment for denature, annealing, extension temperature on the optimal PCR condition was also conducted. The most clear and reproducible bands were appeared in 40ng(1unit taq polymerase and 2.5mM MgCl2, and lunit taq polymerase and 4.5mM MgCl2, 1unit taq polymerase and 7.0mM MgCl2), 60ng(0.5unit taq polymerase and 2.5mM MgCl2, 0.5unit taq polymerase and 4.5mM MgCl2, 1unit taq polymerase and 2.5mM MgCl2, 1unit taq polymerase and 4.5mM MgCl2, and 1unit taq polymerase and 7.0mM MgCl2). The best MgCl2 concentration was 4.5mM(40ng template DNA and 1unit taq polymerase, 60ng template DNA and 0.5unit taq polymerase, and 60ng template DNA and lunit taq polymerase), and 7.0mM(40ng template DNA and 0.5unit taq polymerase DNA). The combinations which showed the best bands from the amount of taq polymerase were 0.5unit(60ng template DNA and 4.5mM MgCl2) and lunit(40ng template DNA and 2.5mM MgCl2, 40ng template DNA and 4.5mM MgCl2, 40ng template DNA and 7.0mM MgCl2, 60ng template DNA and 2.5mM MgCl2, 60ng template DNA and 4.5mM MgCl2, 60ng template DNA and 7.0mM MgCl2). When we consider results from template DNA concentration, MgCl2 concentration, and amount of taq polymerase, 1 condition(40ng of template DNA, 4.5mM MgCl2 and 1unit taq polymerase) was the best combination for the optimal PCR condition. Reaction temperatures for the optimal PCR condition were 84℃, 32℃, 62℃ and 86℃, 35℃, 64℃.

유색미에 관한 연구 : Ⅲ. 유색미 유망계통에 대한 주요 생육 특성

김광수,최윤표,김선택,최현구,정종태,김보경,유지홍,이희봉 충남대학교 농업과학연구소 2007 농업과학연구 Vol.34 No.2

국내외 유색미 수집종을 돌연변이원에 의해 유기된 우수 계통과 수집종간 상호교배에 의해 선발된 CNU 20계통을 파종하여 얻어진 주요 작물학적 특성을 살펴보면 다음과 같다. 1. 간장은 유색 메벼인 CNU126 계통이 대조품종인 동진벼와 같이 가장 컸고 메성인 CNU 128계통에서 가장 작았다. 2. 주당 분얼수는 CNU3, CNU50. CNU56, CNU112 계통에서 가장 많았고 대조품종과 CNU 128 계통에서 10개 미만으로 가장 적게 나타났다. 3. 수장은 육성 계통 중 CNU88 계통에서 가장 길었으나 대조구보다 짧고, 육성계통 중에서는 CNU 126 계통이 가장 짧았다. 4. 주당 영화수는 대조구인 동진벼보다 CNU50 계통에서 2배 이상 많았고, CNU158 계통에서 가장 적게 나타났다. 5. 천립중은 CNU113 계통에서 30g이상으로 대조구와 같이 가장 무거웠고, CNU128 계통에서 20g으로 가장 적었다. 6. 주당 수량은 대조구의 26.6g에 비해 CNU50, CNU112 계통에서 두배정도 높았고, CNU128, CNU158, CNU200 계통에서 가장 낮게 타나났다. Aims of this study were carried out to develop the useful lines induced from mutation and pedigree breeding methods among the collected genetic resources from national and domestic areas. In this study, Stem height of CNU126 line and check among them were high, while CNU128 was lower than other lines. Spiklet length of CNU88 was longer, but that of CNU126 was shorter than check. Number of spiklets per plant of CNU50 among lines have twice time than check. 1,000 grains weight of CNU113 was higher than check. In yield per plant, CNU50 and CNU112 were higher than check, Dongjinbyeo.

토마토의 PCR 적정 조건 연구

김보경,김용철,최인수 밀양대학교 농업기술개발연구소 2000 農業技術開發硏究所報 Vol.4 No.1

The most fundamental step for the experiments such as RAPD is to optimize the PCR condition. The purpose of this study was to optimize PCR condition in tomato. 3×3×3 factorial experiment for template DNA concentration, MgCl2 concentration, and amount of taq polymerase was conducted. Another factorial experiment for reaction temperature(denature, annealing, extension) was also conducted. The most clear and reproducible bands were observed from 60ng(0.5unit taq polymerase and 2.5mM MgCl2, 0.5unit taq polymerase and 4.5mM MgCl2). The best MgCl2 concentration was 2.5mM(60ng template DNA and 0.5unit taq polymerase, 60ng template DNA and 1unit taq polymerase), and 4.5mM(40ng template DNA and 1unit taq polymerase and 60ng template DNA and 0.5unit taq polymerase). And, the amount of taq polymerase for the optimal PCR condition was 0.5unit(60ng template DNA and 2.5mM MgCl2, 60ng template DNA and 4.5mM MgCl2), and 1unit(40ng template DNA and 2.5mM MgCl2, 40ng template DNA and 4.5mM MgCl2, 40ng template DNA and 7.0mM MgCl2, and 60ng template DNA and 2.5mM MgCl2). When we consider results from template DNA concentration, MgCl2 concentration, and amount of taq polymerase, 4 conditions(60ng of template DNA, 2.5mM MgCl2 and 0.5unit taq polymerase; 60ng of template DNA, 4.5mM MgCl2 and 0.5unit taq polymerase; and 60ng of template DNA, 2.5mM MgCl2 and 1unit taq polymerase; and 40ng of template DNA, 4.5mM MgCl2 and 1unit taq polymerase) were best combinations for the optimal PCR condition. Reaction temperature for the optimal PCR condition was 92℃, 36℃, 72℃.

겨울철 제주연안 열수지 특성 : 강설현상 snowfall

김경보,장승민,김태희,우종규,윤용훈 濟州大學校 海洋硏究所 2004 해양과환경연구소 연구논문집 Vol.28 No.1

In other to know the characteristics of the air sea heat flux at on the coastal of Jeju Island. when there was a snowfall in winter. It is used as the basic data are Automatic Synoptic Observation System(ASOS) compiled by Jeju Regional Meteorological Office(JRMO) from 1993 to 2002, and SST data derived from National Oceanographic Research Institute(NORI) and National Fisheries Research & Development Institute(NFRDI) observations. the heat flux of the coastal of Jeju Island shows its maximum in January(latent heat flux : 35Wm^(-2) , sensible heat flux : 85Wm^(-2)), and shows its minimum in July(latent heat flux : -l3Wm^(-2), sensible heat flux : -5Wm^(-2)) the more the temperature gap between the air and SST is large and the stronger a wind is, the more it supplies the heat from the sea to the air. it shows that the north-western areas is about three times higher than other areas in Jeju Island. when fresh snow cover is 0.0∼0.9 ㎝, the mean bowen's ratio(for 1993∼2002) is 0.63∼0.67. when it is 1.0∼4.9 ㎝, the mean bowen's ratio(for 1993∼2002) is over 0.72 or more. When bowen's ratio increased upto 0.63∼0.80. fresh snow cover is increased gradually, therefore, it is researched the there is proportion between the fresh snow cover and the bowen's ratio.

적외선 열화상 카메라를 이용한 Plume 형상에 따른 Nd:YAG 레이저 용접 결함 평가

김재열,유영태,양동조,송경석,노경보 한국비파괴검사학회 2003 학술대회 논문집 Vol.- No.1

In Nd:YAG laser welding, evaluation methods of welding flaw are various. But, the method due to plume shape is difficult to classification of welding flaw. The Nd:YAG laser process it known to have high speed and deep penetration capability to become one of the most advanced welding technologies. At the present time, some methods are studied for measurement of plume shape by using high-speed camera and photo diode. This paper describes the machining characteristics of SM45C carbon steel welding by use of an Nd:YAG laser. In spite of its good mechanical characteristics, SM45C carbon steel has a high carbon contents and suffers a limitation in the industrial application due to the poor welding properties. In this study, plume shape was measured by infrared thermal camera that is non-contact /non-destructive thermal measurement equipment through change of laser generating power, speed, focus. Weld was performed on bead-on method. Measurement results are compared as two equipments. Here, two results are composed of measurement results of plume quantities due to plume shape by infrared thermal camera and inspection results of weld bead include weld flaws by ultrasonic inspector.

분무 열분해에 의한 Ba1-xSrxFe12O19계 자성 미립자의 제조 및 특성 변화

김동현,김광만,김경남,최세영,심인보,이용근 大韓齒科器材學會 2004 대한치과재료학회지 Vol.31 No.1

Magnetic particles with alternating magnetic field are expected to be useful as thermoseeds in hyperthermic cancer treatment, since they can be targeted and confined to the cancer site. Hard magnetic ferrites such as barium ferrite and strontium ferrite are good candidates for thermoseeds of hyperthermia because of their great hysteresis loss. In order to verify the effect of Sr-substitution to Ba-site, synthesized Ba1-xSrxFe12O19 microspheres with various compositions through spray pyrolysis followed by sol synthesis using barium nitrate, strontium acetate and iron nitrate. Coercive force was increased with increasing substitution amount of Sr whereas magnetic saturation was almost constant. Spherical microspheres with average diameter of 11.7~17.0 ㎛ were produced by spray pyrolysis at 400~1000℃. The mean size was dependent on the pyrolysis temperature and flow rate of carrier gas. In order to obtain a single phase of SrFe12O19 crystal, the spray pyrolyzed spheres were undertaken subsequent heat-treatment above 1000℃. During the subsequent heat-treatment, however, spherical microspheres were agglomerated because of necking between particles. Further study has to be continued working on synthesis of pure SrFe12O19 crystal by means of improvement of pyrolysis temperature scheme and extend the heating period.

서울 시내 일부 대학생의 성지식과 성태도에 관한 연구

김보배,노은경,박서영,박영,오승희,전영란,차현진,윤희상,김수지,이자형 이화여자대학교 간호과학대학 2004 이화간호학회지 Vol.- No.38

This study surveyed and analyzed comparatively college students-knowledge' attitude, was to cultivate the right knowledge and attitude on sex, and serve the foundational data for the suitable sex education. The purpose of the study was to identify and examine: 1) students; sexual knowledge and sexual attitude; 2) the differences between variables of gender, age, and self-perceived sexual knowledge in relation to sexual knowledge and sexual attitude; and 3) the relationship between sexual knowledge and sexual attitude. For this study the questionaires were given to 300 students from five different universities located seoul areas. the questionaires were collected Jenuary 8th to 15th in 2004. Two hundred and nigty one samples were analyzed for the study. The adapted SKAT(saxual knowledge & attitude test; Lief & Reed, 1986) was used to determine sexual knowledge and the students' sexual attitude. Collected data were analyzed statistically by means of percentage, t-test, ANOVA test, and pearson correlation analysis with SPSS 12.0 package program. The main results of this analysis are as follows: Participants consisted of male(48.1%) and female(51.9%) which were mainly between the ages of 21.25(71.8%) with an age range of 19 to 30. Knowledge of sexual disease was scored highest of 92.4%, and konwledge of the knowledge of the male and female's anatomy was the lowest at 60.9%. The respondents' level of sexual attitude was tolerant as on average 3.66(on the basis of 5) Relating to the items, the respondents' score of the sexual curiosity was 4.16, sexual behavior 3.85, self-consolation 3.81, aborthon 3.23, virgine 3.19, extramarital experiences 3.17. There were no significant differences in sexual knowledge between male and females, between younger and older students, residence, sexual experience, and how students mainly obtained their sexual knowledge. But a significant difference was also found religion in their sexual attitude(p<.005). In addition, results showed the relationship between sexual knowledge and sexual attitude(P=0.332).

Lutembacher 증후군 환자 증례 보고

김성범,서정주,곽철훈,김상민,이보라,민선경,황은구,김용인,조욱현,최석구 인제대학교 2008 仁濟醫學 Vol.29 No.-

Lutembacher's syndrome is rare combination of mitral stenosis (MS) and atrial septal defect (ASD). The hemodynamic interplay between the MS and ASD leads to wide variation of clinical presentation. Here we describe a 43-year-old female with Lutembacher's syndrome and tricuspid regurgitation with pulmonary hypertension, who underwent direct closure of ASD and tricuspid valvuloplasty successfully. We also reviewed other literatures in an effort to increase awareness of this condition.

배추의 RAPD 적정 조건 구명

김보경,최인수 밀양대학교 농업기술개발연구소 2000 農業技術開發硏究所報 Vol.4 No.1

It is very important to identify the optimized PCR condition for analyses using PCR. The objective of this study was to optimize PCR condition for RAPD analysis in chinese cabbage. 3×3×3 factorial experiment far template DNA concentration, MgCl2 concentration, and amount of taq polymerase was conducted. Another factorial experiment for denature, annealing, extension temperature on the optimal PCR condition was also conducted. The best template DNA concentration was 40ng(1unit taq polymerase and 2.5mM MgCl2, and 1unit taq polymerase and 4.5mM MgCl2, 1unit taq polymerase and 7.0mM MgCl2), 60ng(0.5unit taq polymerase and 2.5mM MgCl2, 0.5unit taq polymerase and 4.5mM MgCl2, 1unit taq polymerase and 2.5mM MgCl2, 1unit taq polymerase and 4.5mM MgCl2, and 1unit taq polymerase and 7.0mM MgCl2). Clear bands were observed from 4.5mM(40ng template DNA and 1unit taq polymerase, 60ng template DNA and 1unit taq polymerase and 60ng template DNA and lunit taq polymerase), and 7.0mM(60ng template DNA and 1unit taq polymerase). The best amount of taq polymerase was 0.5unit(60ng template DNA and 2.5mM MgCl2, 60ng template DNA and 4.5mM MgCl2), and 1unit(40ng template DNA and 2.5mM MgCl2. 40ng template DNA and 4.5mM MgCl2, 40ng template DNA and 7.0mM MgCl2, 60ng template DNA and 2.5mM MgCl2, 60ng template DNA and 4.5mM MgCl2, 60ng template DNA and 7.0mM MgCl2). When we consider results from template DNA concentration, MgCl2 concentration, and amount of taq polymerase, 3 conditions(40ng of template DNA, 4.5mM MgCl2 and 1unit taq polymerase; 60ng of template DNA, 4.5mM MgCl2 and 1unit taq polymerase; 60ng of template DNA, 7.0mM MgCl2 and 1unit taq polymerase) were best combinations for the optimal PCR condition. Reaction temperatures for the optimal PCR condition were 84℃, 32℃, 62℃ and 86℃, 35℃, 64℃.