80年代 農村새마을運動과 行政指導

李鍾益,李殷鎬,李光鍾 청주대학교 새마을연구소 1984 새마을硏究論文集 Vol.1 No.-

The basic ideas of the new community movement were based on the autonomous and voluntary participation of local residents. Since the beginning of the Saemaul Undong in the early 1970, Korean government has been played a major role in igniting and pushing ahead with the new community movement. From intellectual, economic, social, cultural points of view, the new community movement with strong administrative guidlines has attained a lot of achievements during the past 10 years. What is more important, the voluntary participation of local people rather than the administrative directions are urgently needed to activate the rich and affluent villages in the new community movement of the '80. The directions of the desirable administrative guildines in the eighties are as follows; o To convert the directive points into the assistant one in planning functions of local development. o To formulate the group cooperation system in the decision making of local people. o To develop the inventive and suggestive systems of local residents on the new community movement. o To improve the understanding of the mass to make both the national and local development planning together. o To make a suggestion and publish the blue-print of long time development planning.

Helicobacter pylori 편모 유전자의 클로닝 및 염기서열 분석

이광호,이우곤,조명제,도영미,백승철,강경희,박필성,이상룡 경상대학교 유전공학연구소 1993 遺傳工學硏究所報 Vol.12 No.-

A λgt11 expression libary of H. pylori DNA in E. coli Y1090 was screened with flagellin-specific rabbit antiserum for molecular cloning of the flagellin gene of H. pylori. A positive clone, λHPF4, was obtained and the recombinant antigen expressed from λHPF4 was a fusion protein with the molecular weight of 168kd. Sequence analysis of antigen-encoding DNA showed that an open reading frame composed of 1,536 nucleotides encodes a polypepride with a oredicted molecular size of 54kd. This open reading frame did not show the homology with flaA gene encoding 56kd protein of H. pylori and was confirmed as a unique sequence through homoligy searching. Therefore, the cloned antigen is supposed to be the carboxy-terminal region of the other flagellin protein of H. pylori, flaB, with the molecular weight of 58kd.

급성 근골격질환에 있어서 피록시캄 근육주사제의 임상적 연구 : An Open, Non-Comparative Trial

윤승호,이광진,김학영,문형석,조성일 충남대학교 의과대학 지역사회의학연구소 1987 충남의대잡지 Vol.14 No.1

The authors have conducted a clinical study to evaluate the efficacy and toleration with newly developed piroxicam injectable (I. M.) to thirty-one cases of the patients of musculoskeletal disorders during five months from Oct. 1986 to Feb. 1987. Treatment for the patients was initiated with two amples of piroxicam 20mg I. M. daily for first two days and followed administration one ample of 20mg piroxicam daily for three to seven days. Results were as follows: 1. Out of 31 cases, male were 16 (50.5%), female 15(49.5%), and predominant age group was 20-29 years old 11(35.4%). 2. Major indications were sprained ankle and sprained PIP joint disorders 11(35.7%), 12(38.7 %), respectively. 3. Overall efficacy, more than good was 83.8% by patients and 96.8% by physician. 4. Overall tolerance, more than good was 90.3% by patients and 90.3% by physician (excluded four cases). 5. Side effects were seen in 4 patients such as I case of constipation, 2 cases of abdominal pain and 1 case of vomiting. 6. Conclusion: The piroxicam I. M. was very effective and well tolerated for the treatment of musculoskeletal disorders and side effects were few and generally mild.

심근침범을 동반한 Becker형 근이영양증 환자에서 확인한 Dystrophin 유전자 Exon결실

김광일,오병희,이무용,채인호,신수,박성섭,김효수,손대원,이명묵,박영배,최윤식,이영우 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.5

조직항원이 다른 순계동물간의 집락억제현상에 관한 연구 : 종양세포의 집락형성에 대하여 Report on the colony formation of MCA-induced tumor cell

장우현,이광호 최신의학사 1975 最新醫學 Vol.18 No.6

Sarcomas in C3H/HeN, C57BL/6 and DDO mouse strains were induced by subcutaneous injections of 20-methylcholanthrene and cultivated in vitro. Results on tumor transplantation and colony formation on Petri dishes are summarized as follows; 1. l mg of subcutaneous injection of methylcholanthrene induced tumors in C3H/HeN, C57BL/6 and DDO mice after 8 weeks. The frequency of tumor induction in C3H/HeN was the highest. Histologically, the tumors appeared as sarcomas with numerous mitotic figures. 2. Trypsinized cell suspension of MCA-induced sarcoma of C3H/HeN were transplanted to 5 mice of C,H/HeN and palpable tumors appeared in 20 days among all of the mice. Histological finding were identical with those of the original tumor. 3. Trypsinized monolayer cell suspensions of C3H/HeN MCA-induced sarcoma culture were transplanted to 4 mice of C3H/HeN and palpable tumors arose among all of the mice within 30 days. Histological findings were identical to that of the original tumor. 4. Complete Waymouth's Medium has been satisfactory for the establishment of MCA sarcoma cell line in vitro and maintenance of the subculture at 37°C, 5% CO2 incubator. 5. Cloning efficiencies in the cases of 104 cells/dish, 103 cells/dish and 102 cells/dish were 2.7~2.8%, 7~9% and 8~10%, respectively. The diameter of colonies was 3~6 mm as a whole.

圓光醫大 附屬 第2病院 神經精神科 患者에 對한 統計的 考察(Ⅱ)

박민철,장광철,김상원,김한주,유미경,김훈,유용진,김현정,박남진,오상우,이귀행,노승호,백영석 圓光大學校 醫科大學 神經精神科學敎室 1992 圓光精神醫學 Vol.8 No.1

본 연구는 1986년 7월 11일부터 1991년 7월 10일까지 원광의대 부속 제2병원 신경정신과에서 의래 및 입원치료를 받았던 6,072명의 환자를 대상으로 병원 역학조사를 실시하고 그 결과를 1차조사 (박민철 등1986)와 비교했다. 1.전체 환자 중 남자가 54.4%로 여자보다 많았고, 이리와 이리 인접지역이 84.0%로 대부분의 지역을 나타났다. 의료보장상태는 보험환자가 57.1%, 의료보호환자21.8%, 일반환자 21.1%였으며 연령별로는 20대 24.6%, 30대 22.8%로 20대 30대가 절반정도를 차지했다. 2.외래환자에서는 신경중성장애가 46.0%로 가장 많고 정동장애, 경련성장애 순이며, 입원환자에서는 정신분열증이 30.5%로 가장 많고 기질성정신장애, 정동장애의 순이었다. 3.남자는 신경증성쟝애(23.4%), 정신분열증, 기질성정신장애 순이고 여자는 신경증성장애(39.9%), 정동장애, 정신분열증의 순이었다. 남자가 여자보다 많은 진단은 알코올정신장애(94.6%), 기질성정신장애, 소아정신장애 순이고, 여자가 남자보다 많은 진단은 정동장애(64.5%), 신경증성장애였다. 4.진단별 연령분포에서 신경증성장에는 30대(25.3%), 20대, 40대 순이고, 정신분열증은 20대(40.5%), 30대, 40대 순이며, 정동장애는 20대, 30대, 50대 순이나 비교적 비슷한 융이었다. 5.진단별 발병연령에서 신경증성장에는 20대, 30대가 절반이고,정신분열증은 20대(51.3%), 10대, 30대 순이며 정동장애는 20대(30.7%), 30대, 50대 순이었다. 진단별 평균 발병연령은 신경증성장애 32.7세, 정동장애 36.1세, 정신분열증 23.6세였다. 6.진단별 이병기간은 2년이상이 신경증성장에 28.3%, 정신분열증 68.5% 정동장애 43.9%인데 전체적으로 41.0%였다. 진단별 평균 이병기간은 정신분열증 73.2개월, 정동장애 45.5개월, 신경증성장애 29.5개월이었다. We examined epidemiologic study of 6,072 patients who visited Wonkwang Neuropsychiatric hospital since July 11 of 1986 until July 10 of 1991 and compared this results with previous study(Park et al 1986). The study revealed as follows:1. Of total patients males visited this hosptial more than females as 57.1%, Most of them(84.0%) resided Iri and neighbouring Iri. In the point of insurance, insured patients, medicaid patients and uninsured patients were 57.1%, 21.8% and 21.1% respectively. On the distribution of age, the twenties, the thirties were 24.6%, 22.8% respectively and twenties and thirties were about half of total patients. 2. Of outpatients neurotic disorder was the most as 46.0%, secondly mood disorder and thirdly convulsive disorder, but of inpatients schizophrenia was the most as 30.5%, secondly organic mental disorder and thirdly mood disorder. 3. Of males neurotic disorder was the most as 23.4%, secondly schizophrenia, and thirdly organic mental disorder, but of females neurotic disorder was the most as 39.9%, secondly mood disorder and thirdly schizophrenia. alcoholic mental disorder(94.6%), organic mental disorder, child mental disorder were mostly found in males,but mood disorder(64.5%) and neurotic disorder were mostly found in females. 4. On the distribution of age neurotic disorder was found the most in the thirties(25.3%), next in the twenties and in the forties, and schizophrenia was found the most in the twenties(40.5%), next in the thirties and in the forties, but mood disorder was found the most in the twenties, next in the thirties and in the forties but revealed similar rates relatively. 5. On the distribution of age of onset neurotic disorder developed nearly half during the twrnties and the thirties, schizophrenia developed mostly during the twenties (51.3%), secondly the teens and thirdly the thirties, but mood disorder developed firstly the twenties(30.7%), secondly the thirties and thirdly the fifties. on the average age of onset of illness neurotic disorder, mood disorder and schizophrenia were 32.7 years, 36.1 years and 23.6 years respectively. 6. On the duration of illness more than two years was 28.3% in neurotic disorder, 68.5% in schizophrenia and 3.9% in mood disorder and on the average duration of illness schizophrenia, mood disorder and neurotic disorder were 73.2 months, 45.5 months and 29.5 months respectively.

優良室素固定菌의 探索分離 및 生理的 特性

朴愚喆,李光熙,金進鎬,李麟九,曺晋基 慶北大學校出版部 1987 慶北大農學誌 Vol.5 No.-

This experiment was conducted to search for Rhizobia with good nitrogen fixation abilities and to investigate their physiological characteristics isolated from 12 soybean cultivars and the affinities of root nodule bacteria with soybeans. The results obtained were as follows ; Based on colors, Rhizobia grown on YMA medium were divided into 3 groups, i. e., white, translucent and transparent, amounting to 60, 30 and 10%, respectively. In litmus milk reation, the strains which produced alkali, acid serum, alkaline serum and acid reached to 51, 29, 9 and 11%, respectively. Strains, S022, and S096 were slow-growers and produced alkaili, while strains, S080, S090, and S118 were fastgrowers and produced acid. The growth of root nodule bacteria on YMA medium was favorable between the initial pHs of 6.0~7.0. Glutamine, asparagine and allantoin as nitrogen sources enhanced the growth of root nodule bacteria. All the strains tested formed nodules on the soybean roots, and the strains with good symbiotic nitrogen fixation abilities that had white color, small colony, nitrate reduction abilities and no nitrite reduction abilities showed comparatively high nitrogen fixing activities. Some strains varied in nitrogen fixing activities according to soybean cultivars, and a few strains formed ineffective nodules which showed no nitrogen fixing activity.

정형회과영역에서의 Cefmetazole(Cefmetazon^(�)) 임상적 효과

이정웅,문형석,안상로,이준규,이광진,윤승호 최신의학사 1988 最新醫學 Vol.31 No.5

정형외과 영역에서 Sulperazon(Sulbactam/cefoperzon)의 Non-Comparative study

신현대,임상덕,안상로,이준규,이광진,윤승호 최신의학사 1989 最新醫學 Vol.32 No.2

Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.