국산 Lidocaine 알칼리화를 위한 Bicarbonate 혼합시 침전유발 최소량에 대한 고찰

이선호,김현수,곽인숙,안원식,최관호,김광민,손민제 대한마취과학회 2000 Korean Journal of Anesthesiology Vol.39 No.5

Background : When local anesthetics for regional anesthesia is used, usually small amounts of bicar-bonate are added for rapid onset. This addition gives the mixed solution a more alkaline pH. The following result is an increased unionized form of the local anesthetic and rapid penetration of the drug into tiissue. Unfortunately, no data about adequate mixing volumes of domestic lidocaine and bicarbonate is available. Methods : We examined six mixing pairs of two kinds of 2% lidocaines and three kinds of 8.4% bicarbonates for minimum volumes of bicarbonate to cause a precipitation of 2% 20 ml lidocaine. Results : The mean volumes of bicarbonate to cause precipitation were 1.54 ml for Kwang-Myung lidocaine with Kwang-Myung bicarbonate, 2.90 ml for Kwang-Myung lidocaine with Dae-Won bicarbonate, 2.73 ml for Kwang-Myung lidocaine with Je-Il bicarbonate, 0.97 ml for Je-Il lidocaine with Kwang-Myung bicarbonate, 1.26ml for Je-Il lidocaine with Dae-Won bicarbonate and 1.39 ml for Je-Il lidocaine with Je-Il bicarbonate. Conclusions : We conclude that the Kwang-Myung lidocaine and the Je-Il lidocaine could cause precipitation when mixing with a smaller bicarbonate volume than foreign textbook recommended. (Korean J Anesthesiol 2000; 39: 726-729)

Fluphenazine Decanoate의 治療經驗 第 3 篇 錐體外路症床과 經口用 抗精神病藥物의 治療經歷

金光日,金容植 大韓神經精神醫學會 1980 신경정신의학 Vol.19 No.4

The authors have tried to evaluate the incidence of extrapyramidal side reactions between acute schizophrenic and chronic schizophrenic groups in converting of oral antipsychotic medications into fluphenazine decanoate injection. The acute group consisted of 15 acute schizophrenics whose history of previous oral antipsychotic medication was less than 3 months. And the chronic group was 24 chronic schizophrenics whose history of, oral medication was more than one year. Ayd's method was applied to the all patients; in converting of oral antipsychotics into the depot form, the full estimated dose of fluphenazine decanoate was given for the initial shot after two days of clearance of oral medications. DiMascio's rating scale was used for evaluation of extrapyramidal symptoms. Extrapyramidal symptoms such as rigid facial expression, tremor, akinesia, rigidity, akathisia and dystonia were noticed to be 80 percent in the acute group and 45.8 percent in the chronic group. This difference was significant on the level of 0.02. It was proved that the patient group with a long-term history of previous oral antipsychotic medication revealed a lower incidence of extrapyramidal side reactions compared to the group with a shorter history of previous oral medication. For a safer dosage regimen even in the chronic patient group, however, it is recommended to start the regimen with very small test dose and to increase gradually up to 1cc as required, because this low-dose regimen manifested extrapyramidal symptoms in 10 or 19 percent from the previous trials(Kim-1979, Kim & Kim-1980).

유명산의 식물상 및 산림군락분류에 관한 연구

김흥식,김인수,홍금희,김남영,박광서,김정유,박완근 江原大學校 森林科學硏究所 2003 Journal of Forest Science Vol.19 No.-

2002년 3월부터 2003년 10월까지 유명산 자연휴양림을 대상으로 식물상과 산림군락구조를 조사한 결과, 관속식물이 98과 331속 570종 1아종 81변종 4품종 2교잡종으로 총 658종류로 나타났으며, 한국특산식물은 10과 14속 13종 2변종 1교잡종 총 16종류. 희귀 및 멸종위기식물은 9과 10속 11종 총 11종류, 귀화식물은 12과 22속 28종 총 28종류로 나타났다. 산림군락은 신갈나무-철쭉군목, 신갈나무-생강나무군단, 소나무-산거울군락, 잣나무-주름조개풀군락, 물푸레나무-신갈나무군락, 층층나무-신갈나무군락, 굴참나무-신갈나무군락, 싸리-신갈나무군락으로 전체 1군목, 1군단, 6군락이 분포하는 것으로 나타났다. The study was carried out to investigate the flora and community classification of forest vegetation in Mt. Yumyeong from March, 2002 to October, 2003. As a result of that, the vascular plants were 98 families, 331 genera, 570 species. 1 subspecies, 81 varieties, 4 formae, 2 hybrid, 658 taxa in all. Korean endemic plants were 10 families, 14 genera, 13 species, 2 variaties, 1 hybrid. 16 taxa in all ;, rare and endangered plants were 9 families, 10 genera. 11 species, 11 taxa in all ; naturalized plants were 12 families, 22 genera, 28 species, 28 taxa in all. The forest vegetation was classified into 1 order, 1 alliance. 6 communities : Rhododendro - Quercetalia mongolicae (Kim, 1990) Lindero - Quercion mongolicae (Kim, 1990) A. Carex humilis var. nana - Pinus densiflora Community B. Oplismenus undulatifolius - Pinus koraiensis Community C. Fraxinius rhynchophylla - Quercus mongolica Community D. Cornus controversa - Quercus mongolica Community E. Quercus variabilis - Quercus mongolica Community F. Lespedeza bicolor - Quercus mongolica Community

서로 다른 CNC 환경을 갖는 공작기계의 인터넷 기반 점검

김동훈,김선호,김경돈,김찬봉,고광식 한국공작기계학회 2004 한국공작기계학회 춘계학술대회논문집 Vol.2004 No.-

This paper proposes Internet-based checking scheme for machine-tools with a variant CNC. Checking points defined in this research are classified into two categories such as structured point and operational point. The formal includes the vibration of bearing, temperature of spindle unit and another periodical management. And the latter includes oil checking, clamp locking/unlocking and machining on/off status. Through this research, for the global management of variant CNC machines interspersed with CAC and OAC in manufacturing system, the suitable environment for Internet-based checking of variant CNC machines was designed and the checking methods for CAC and OAC machines were compared with each other.

랫트의 생체내 카드뮴 단독투여 및 카드뮴과 셀레늄 병용투여에 따른 필수원소의 변화

김동현,변정식,조영채,이범식,김광환,김정윤 忠南大學校 環境問題硏究所 1995 環境硏究 Vol.13 No.-

This study was performed to find out the changes of essential elements as Cu, Fe, Zn, and Ca in blood, liver, kidney and testis of rats. The experimental rats were divided into 4 groups that were consisted of control group(groupⅠ), 3.0㎎Cd/kg group(groupⅡ), 3.0㎎Cd +1.0㎎Se/kg group(groupⅢ) and 3.0mgCd+2.5mgSe/kg group(groupⅣ). Each group was intraperitoneal injection with difference dose of cadmium and selenium such as 3.0mgCd/kg, 3.0mgCd+1.0mgSe/kg and 3.0mgCd+2.5mgSe/kg. Rats were killed at the time of 3 day and 28 day, and Cd, Se, Cu, Zn and Ca concentration in several organs were determined by Atomic Absorption Spectrophotometry and inductively Coupled Plasma. Cadmium content in blood, liver, kidney and testis were significantly increased in the Cd alone injection group than Cd mixed with selenium injection groups. But, copper content were not significantly differences in control and each experimental group. Iron content were significantly increased in the Cd alone injection group than others groups. Zinc and calcium content were significantly decreased in the Cd alone injection group than other groups. On the other hand the essential element content in Cd mixed with selenium injection group were almost equaled with those of control group than Cd alone injection group.

Mushroom堆肥의 飼料化에 關한 硏究 : 第1報 Mushroom 堆肥腐熟過程中 몇가지 有機成分과 腐植化度 Amino酸 및 酵素活性의 經時的 變化 THE VARIATION OF GENERAL ORGANIC COMPOSITION, DEGREE OF HUMIFICATION, FREE AMINO ACID AND ACTIVITY OF AMYLASE IN THE COMPOST BEFOR CULTIVATION OF MUSHROOM AND IN MUSHROOM COMPOST

李殷喆,金銅淵,金容植,金廣植 全南大學校 農漁村開發硏究所 1966 農業科學技術硏究 Vol.4 No.-

In order to use mushroom compost as feedstuffs to exeriments were carried out to determin the variation of general organic composition, degree of htunification, free amino acid and activity of amylase in the compost before cultivation of mushroom and mushroom compost. The results obtained were summarized as follows; 1. Contents of crude protein and soluble protein of the compost for mushroom cultivation were increased. In the mushroom compost crude protein contents were 10.9% and soluble proteins were 1.95%. The contents of nitrogen free extract were decrcased in both compost. Crude fiber contents were increased during fermentation but in mushroom compost contents of crude fiber were markedly decreased from 23.7% to 12.4%. 2. Degree of humification during fermentation was increased but in mushroom compost was decreased. 3. The free amino acid which were extrated with alfohol in mushroom compost were identified. that is cysteine, Serine, glycine, threonine. alanine, tynosine, methionine and isoleusine. 4. The value of amylase activity from mushroom compost was about two tims higher than 10 days fermented compost.

세포질내 정자주입법(ICSI)에 있어서 정자흡입 및 난자내 주입방법에 관한 연구

이택후,김항진,송건호,김대근,전상식,박윤규,서태광,전병균,류은경,이은숙,문진수,김광철 경북대학교 의학연구소 2000 경북대학교병원의학연구소논문집 Vol.4 No.1

Study on Method of Sperm Aspiration and Injection into an Oocyte in Intracytoplasmic Sperm Injection(ICSI) Immobilization of spermatozoa prior to intracytoplasmic sperm iniection(ICSI) sometimes results in crooked tail and this makes it difficult to aspirate sperm into an injection pipette tail first. Head-first sperm aspiration into an injection pipette avoid this problem due to the bigger size of the sperm head. The effect of head or tail-first sperm injection into an oocyte on fertilization cleavage, percentage of grade I embryos and development to blastocyst stage in ICSI program has been studied. A single living immobilized spermatozoa from oligoasthenozoospermic patient was injected into an oocyte head-first or tail-first according to the treatment. Eighteen hours after microinjection, oocytes ware inspected for survival and fertilization Fertilized oocytes with two pronuclei were cultured in 30μl drop of mHTF supplemented with 10% heat-inactivated follicular fluid(FF) at 37℃. On day 2. embryo transfer was performed with cleaved embryos. The remaining 2-8 cell stage embryos were co-cultured with BRL cells in mHTF + 10% FF for 72 hours and the developmental stage was observed. The data were analyzed by Analysis of Variance. A total of 164 oocytes from 36 cycles were assigned to earth treatment and ICSI was performed(88 head-first, tail-first). The rates of normal fertilization were 81.8% and 76.3% for head-first and tail-first, respectively. Of the fertilized oocytes, the percentage of cleaved embryos and the percentage of grade 1 embryo among cleaved embryos were 88.9% and 68.8%, 93.1% and 74.1% for head-first and tail-first, respectively. Of the 2-8 cell embryos cultured, 44.4%(16/36) and 50.0%(10/20) for head first and tail first, respectively developed to blastocyst stage. There were no differences in fertilization, cleavage, rates of grade 1 embryos, and development to blastocyst stage. In conclusion, head-first or tail-first sperm injection into an oocyte in ICSI program does not affect fertilization and subsequent embryo development to blastocyst stage in vitro.

독성물질의 세포사 기전 및 세포사 유발물질의 검색법 개발에 관한 연구(Ⅰ) : 독성물질로 인한 파킨슨병 모델에서의 세포사 기전 연구 Study on the cell-death mechanisms of toxin-induced parkinsonism

강태석,김종민,서경원,김영옥,김준규,오재호,이윤동,김규봉,오정자,송연정,임종준,전범석,문전옥,최광식 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-

MPTP 독성물질이 도파민성 신경세포에 선택적으로 작용하여 산화성 손상에 의한 신경세포사를 일으키는 것을 이용하여 파킨슨병의 동물모델을 만들고, 이를 통해서 아폼토시스를 비롯한 포사의 기전에 대한 연구 및 너코틴의 신경세포 보호효과 여부를 판정하는 실험을 병행하고자 하였다. 파킨슨꾐의 동물모델을 MPTf 독성 물질을 이용하여 확립하였으며, MPTP(30mgag, i.p.)를 투여한 후 1, 2,3, 4, 5일째 흑질 조직을 채춰하여 tarm로 박걸하여 tyrosine hydroxylase 면역조직화학염색을 수행하여 cell countif우한 결과, control은 57.635ce11s, 1일째 친.OfDells,2일째 57.9±6cells,3일릴 없.3±죠ells, 4일째 49.0츠3cells, 5일째 39.4±Scells료 4, 3일째 뚜렷한 신경세포 수의 감소를 보였다. 신경세포사 기전 규명을 위한 아폼토시스 분걱에서는 벼PTP 투여 후 1, 2, 3, 4, 5일째 조직을 채취하여 Hoechst staining, TUNEL staining을 수곡하였는데 양성 반응을 보인 신경세포는 관찰되지 않아. 아폼토시스로 인한 세포사가 관찰되지 않았다. bIPTP 파킨슨병 동물모델에서 nicotine 보호효과 탐색에 관한 실험은 nicat푸e 0.2mgAg을 5일 퐁안 투여 후 리『fP(30mgag)를 CS7Bt/6 마은스에 복강 내주사로 nicotine과 병용 투여한 후 1, 2, 3, 4, 5일째 뇌를 적출하땄다. 신경세포사가 뚜렷이 관찰되기 시작하는 4, 5일째의 신경세포 수의 감소 정도를 20. 30% 정도 약화시키는 경향을 보였으나, nicotine 보호효과에 대한 추가 실헝이 현재 수행 중에 있다. The cause of Parkinson's disease (PD) is largely unknown. However, free radical toxicit? may plaf a role ip. the degeneration of substantia nigra, which is the Hajorfocus of pathological damages in PD. Recently, a neuroprotective effect of nicotine in PD has been suggested. Therefore, the mechanism of neurodegenerafion and protective potential o( nicotine in PD were investigated in the experimental modeB of Pll using a neurotoxin, C57BL/6mice were administered with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP, 30 mg/kg,j.p.). The degree of neurodegenerafion was determined by immunohistochemical stainiHB oftyrosine hydroxylase (TH). TH-positive cells on nigral sections were found 56.0 ±4, 57.9 ±6,52.315ce11s, 49.0±3cells, and 39,4±Scells at days 1, 2, 3, 4, 5, respectively (controls : 57.6±Scells). Hoechst and TUNEL staining showed no evidence of apoptosis. The exandnation on themice co-adrunistered with nicotine(0.2mgAg) and MPTP(30mgag) revealed a tendency ofnicotine protective effects. At days 4 and 5, the degree of TH-positive cells was decreased by20-30%, In corclusiffn, the role of apoptosis was not evidenced in this MPTP modeB of PB.The possible proteccon by nicotine should be elucidated with further studies.

당뇨병 백서의 간세포에서 Glucokinase 활성도 및 유전자 발현에 대한 인슐린의 영향

강성이,팽정령,서광식,안규정,우정택,김성운,양인명,김진우,김영설,김광원,최영길 경희대학교 유전공학연구소 1993 遺傳工學論文集 Vol.5 No.-

목적 당대사의 조절 상태에 따른 생체 변화를 분자 수준에서 이해하고자 식이 조건을 달리한 정상 백서와 화학적으로 유도된 당뇨병 백서의 간조직에서 혈당수준과 인슐린치료 정도에 따라 나타나는 글루코키나제 활성도 및 유전자 발현을 분석하였다. 방법 스트렙토조토신 정맥투여 후 당뇨병의 유발을 확인하고, 인슐린을 1일 3회 3일간 복강내로 투여하여 상태를 안정시킨 후, 인슐린 투여군은 인슐린 투여 6시간이내에 그리고 인슐린 비투여군은 24시간 후 단두하여 채혈하고 복강을 열어 간조직을 채취하였다. 채취한 간조직에서 글루코키나제 활성도는 인산화된 포도당에서 NADH의 형성을 형광분광계로 측정하였으며, 글루코키나제 유전자 mRNA발현은 Northern 분석법을 이용하였다. 성적 정상 백서에서 공복상태와 식이를 섭취한 경우에 간조직의 글루코키나제 효소의 활성은 차이가 없었으나, 글루코키나제 유전자 mRNA 발현은 증가되었다. 당뇨병이 유발된 백서의 간조직에서 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA 발현은 정상 백서에 비하여 낮았다. 인슐린 투쳐 후 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA 발현이 증가되었고, 특히 혈당이 정상화된 경우에서 글루코키나제 유전자의 mRNA 발현이 증가도었다. 결론 인슐린에 의한 간조직에서 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA을 증가를 볼수 있었다. 당뇨병 백서에서 인슐린 투여 후에 혈당조절이 안된 경우 간조직의 글루코키나제 유전자의 mRNA 발현이 증가가 없는 것으로 보아 글루코키나제 mRNA의 발현에는 인슐린 이외의 다른 요소가 관여할 것으로 생각된다. The liver-specific hexokinase isoenzyme, referred to as glucokinase, is thought to play a key reglulatory role in hepatic glucose metabolism. The glucokinase gene is, therefore, of interest both because of its tissue-specific expression and because of the several regulatory processes that can be analyzed. The level of hepatic glucokinase activity appears to be determined essentially by regulation of the rate of enzyme synthesis, with insulin playing a leading role as an inducer. We investigated the role of insulin for the induction of glucokinase in the liver of diabetic rats. Experimental diabetes was induced by injection of streptozotocin 7 days before the experiment. Regular insulin was given by three days intraperitoneal injection at 8-h interval. The glucokinase mRNA in the liver was estimated by Nothern blot assay, as well as by fluorometric enzyme activity assay. Glucokinase activity was not reduced in the liver of normal fasting rats as compared to normal fed rats. And glucokinase activity was reduced in the liver of diabetic rats as compared to normal rats. In diabetic rats treated with insulin, glucokinase enzyme activity were increased. But glucokinase mRNA expression was only increased in normoglycemic diabetic rat with treated with insulin as compared to hyperglycemic rat. These data indicate that insulin stimulates hepatic glucokinase enzyme activity and mRNA expression. But other hormonal or metabolic factors may be contribute to regulation of glucokinase mRNA expression.

Craves병 환자에서 백혈구와 갑상선 조직의 HLA-DR β 유전자 부위의 비교

양인명,우정택,팽정령,서광식,김성운,김영설,김광원,최영길 경희대학교 유전공학연구소 1989 遺傳工學論文集 Vol.1 No.-

정상 갑상선세포에서는 HLA-DR 항원이 발현되지 않았으나, 그레이브스병 환자의 갑상선세포 표면에는 HLA-DR 항원이 발현됨이 보고되어, 이러한 현상은 이 질환의 자가면역 기전에 중요한 역할을 하고 있을 것으로 생각되고 있다. 한편 최근 DR이나 DQ 유전자의 상부 159kb 이내에는 이들의 발현을 조절하는 유전자가 존재함이 알려져 있고, 이 부위의 구조적인 변화로 인하여 DR DQ 유전자의 발현을 유도하는 여러 가지 핵내 인자들과 interferon-r 와 같은 외부인 자들이 보고되고 있다. 그러나 그레이브스병에서 이들 유전자 부위의 구조적인 변화에 관해서는 아직 보고가 없다. 이에 본 연구자 등은 이러한 가능성 여부를 규명하고자 2명의 전형적인 그레이브스병 환자의 수술 우 얻어낸 갑상선 조직에서 RNA를 분리한 우 DR β유전자를 소식자로 northem blotting을 하여 mRNA의 발현을 관찰하였으며, 말초혈액 백혈구와 갑상선 조직으로부터 분리된 DNA를 EcoRI BamHI. HindⅢ PvuⅡ TaqI, PstI등의 6가지 제한효소로 소화한 후 DR β유전자를 소식자로 하여 RFLP 양상을 비교한 결과, 환자 모두에서 mRNA의 발현이 관찰되었으나, 환자 모두에서 6가지 제한효소에 의한 RFLP 양상이 동일하였다. 이러한 결과는 Graves병 환자의 DR 유전자의 발현에 있어서 이 유전자 부위의 구조적인 변화가 관여할 가능성이 적음을 시사하는 사실이라고 사료되나 향후 더 많은 예와 더 많은 제한효소를 이용한 주시가 필요할 것이다. The requirement for major histocompatibility antigen class Ⅱ molecules in the recognition of antigen by helper T cells suggests that the expression of class Ⅱ antigen may be important in the initiation and prolongation of immunopathology. HLA class Ⅱ antigenes are expressed on the surface of thyrocytes of the patients with graves disease. The increased expression of class Ⅱgene can be induced by trans acting factor such as interferon However the possibility of rearrangement of their regulatory genes has not been explored so far. We studied the mRNA expression in the thyrocytes of 2 patients with Graves' disease and compared the restriction fragment length polymorphism (RFLP) between thyroid and peripheral leukocyte DNA. The prominent expression of mRNA was observed in the thyroid tissues of all the two patients. But we did not find any difference in RFLP pattern in both patients. These results suggest the possibility that the rearrangement of the regulatory gene located in the upstream of DR- β gene can be a role in expression of DR antigen is less likely.