Metarrhizium anisopliae(Metschn.) Sorok이 생산하는 Biopolymer YU-122의 생산과 그 특성

최용석,옥승호,유주현,배동현 한국산업미생물학회 1997 한국미생물·생명공학회지 Vol.25 No.1

토양으로부터 분리한 Metarrhizium anisopliae (Metschn.) Sorok이 생산하는 biopolymer를 정제하여 분석하고, 그의 생산조건을 검토하였다. 탄소원으로 mannitol, 질소원으로는 polypepton을 사용했을 때 가장 많은 biopolymer를 생산하였고 K_2HPO_4 및 CaCl_2를 첨가함으로서 생산량이 더욱 증가하였다. 또한 이 biopolymer를 정제하여 그 분자량과 구성성분을 검토한 결과 Metarrhizium anisopliae(Metschn.) Sorok이 생산하는 Biopolymer YU-122는 분자량이 1.7×10 exp (6)이고, C, H의 구성비가 1:2이며 미량의 N이 검출되었으나, 주 구성성분은 glucose와 galactose의 환원당으로 이루어져있는 전혀 새로운 biopolymer인 것으로 나타났다. To produce biopolymer, Metarrhizium anisopliae (Metschn.) Sorok was cultured in a medium containing glucose 1.0%, sucrose 2.0%, soluble starch 1.0%, yeast extract 0.5%, polypeptone 0.5%, K_2HPO_4 0.1%, MgSO_4·7H_2O 0.02%. The culture broth was centrifuged and the polymer was harvested by adding methanol to the culture supernatant. When three times of methanol was added, the polymer was coagulated and precipitated. Then it was further purifued through successive SK-1B, SA-20P, HP-20 column chromatographies. This polymer was designated as Biopolymer YU-122. C:H ratio of this Biopolymer YU-122 was 1:2 and small amount of N is detected by CHN analyzer. Glucose and galactose are main components of this polymer. Average molecular weight of this biopolymer was 1.7×10 exp (6) by Sepharose 4B gel permeation chromatography. Optimal condition for biopolymer production was investigated. When 5% of mannitol was used as a carbon source, and polypepton as a N source, highest productivity of biopolymer was achieved. C/N ratio as nutrient was also a major factor in polymer production and its optimal ratio was 3.

人參成分 抽出에 관한 硏究

柳洲鉉,卞裕亮,劉承坤 연세대학교 산업기술연구소 1978 논문집 Vol.8 No.1

The amount of ethanol extract from ginseng was about 20.3% (w/w)and the yield of saponins from the extract was 5.5% (w/w). The optimum concentration of solvent was 60% ethanol. The optimum extraction time and temperature were respectively 2 hr and 80℃ when 0.5g of sample (Φ:0.25∼0.42mm) was added to 300ml of solvent. The empirical equation for the extraction of components was as follow: Y(θ,T,φ)=8log(Θ??100)-45log(T??×100)-60log(Φ??×100)+38 The partical size of sample had largely influence on the extraction yield, and the extraction mechanism was considered as diffusion.

견고 구외 골신장술을 이용한 구순구개열 환아의 치험례 : CASE REPORT

유난영,박호원,,김성민,이주현,서현우 大韓小兒齒科學會 2005 大韓小兒齒科學會誌 Vol.32 No.4

구순구개열 환자에서는 이른 시기에 시행된 수술로 인한 구순이나 구개부의 반흔 형성으로 섭식장애나 발음장애를 동반한 상악의 열성장이 나타나게 된다. 때로는 집중적인 교정치료 후에도 심한 상악골 저형성증을 보이며, 이러한 경우 성장이 완료된 후에 골이식을 동반한 악교정 수술로 상악골을 전방이동시켜 안모의 개선을 도모하기도 한다. 그러나 이러한 상악골의 전방이동은 연조직의 과도한 신장으로 인한 술후회귀현상, 추가적인 골이식이 필요하다는 한계를 가지고 있다. 골신장술은 이러한 한계점을 극복하는 최신 치료방법으로 구순구개열 환자, 두개골 융합증을 나타내는 환자 등에서 상악골을 포함한 두개 안면골의 개선에 많이 이용되고 있다. 특히 구외장치를 이용하는 골신장술은 골신장기간 중에 견인 방향의 조절이 보다 쉬우며, 충분한 양의 골신장이 가능하여 보다 좋은 결과를 얻을 수 있다. 본 증례는 상악 열성장을 보이는 구순구개열 6세 7개월의 여자 환아로, 변형된 구내 프로씨 1형 골전단술(modified Le Fort I osteotomy) 후 두개골을 고정원으로 이용하는 견고 구외 골신장술을 통해 상악골을 전진시켜 양호한 결과를 얻었기에 이를 보고하는 바이다. Patients with cleft lip and palate present severe maxillary hypoplasia due to scar of lip and palate, often accompanied by compromised mastication, speech abnormalities. Sometimes maxillary hypoplasia persist even though active orthodontic treatment was done. In theses cases, patients born with cleft lip and palate will be potential candidates for maxillary advancement with bone grafting after growth to correct the functional deformities and improve aesthetic facial proportions. But, maxillary advancement using standarf surgical approaches has several limitations: increased relapse tendency after maxillary advancement, necessity of additional bone graft and mandibular setback surgery. Distraction osteogenesis is current treatment modality to overcome these limitations, thus has become popular for treatment of maxillary hypoplasia associated cleft lip and palate, craniosyntosis. Especoally, rigid external distraction, contrary to internal device, has adbantages; better vector control of osteotomized segment, effective traction of the bony segments, the ease of the application and removal the distraction device. This study showed that relatively successful result could be generated by using rigid external distraction osteogenesis(RED) in the case of cleft lip and palate with severe maxillary hypoplasia, 6 years 7 months old.

소의 시상하부내에서 Vasopressin과 Oxytion분비세포의 분포에 관한 면역조직화학적 연구

유명철,김종중,장인엽,문정석,정주현,김흥중,조사선 朝鮮大學校 附設 醫學硏究所 1991 The Medical Journal of Chosun University Vol.16 No.1

This study was done to observe the distributions of Oxytocinergic and Vasopressinergic neurons in the hypothalamus of the cow. For the immunohistochemical staining, sections were reacted with the following sequence of solutions: monoclonal anti-vasopressin and anti-oxytocin, biotinylated anti-mouse IgG, avidin-biotin peroxidase complex, and Ni-DAB. The results observed under light microscope were summarized as follows. Our immunohistochemical investigations reveal both vasopressinergic and oxytocinergic neurons in the supraoptic nucleus(SON), paraventricular nucleus(PVN), accessory supraoptic nucleus (ASN), periventricular nucleus(PN), suprachiasmatic nucleus(SCN), and arcuate nucleus(AN), but these two types of neurons were mainly distributed in the PVN and SON and the ratio of the number of vasopressinergic neurons to that of oxytocinergic neurons was 1:1.30 in the PVN, and 1:1.00 in the SON. These two types of neurons were round, oval and spindle-shaped and cell size was 30-55um.

구강내 미생물에 대한 서양산 고추냉이(Armoracia rusticana) 뿌리 추출물의 항균효과

유난영,박호원,이주현,서현우 대한소아치과학회 2006 大韓小兒齒科學會誌 Vol.33 No.3

Verticillium sp.가 생산하는 cellulase의 정제 및 그 성질

유주현,홍대희,양융,진효상 연세대학교 산업기술연구소 1986 논문집 Vol.18 No.1

The cellulase of verticillium sp. was purified to investigate its some properties. The enzyme purification was carried out by ammonium sulfate fractionation, and column chromatography of Sephadex G-50, G-100 and DEAE Sephadex A-25, with its specific activity increased to 17 fold. The molecular weight was estimated to be 46,000 by gel filtration. The optimum pH and temperature were pH 7 and 50℃, respectively. The enzyme was stable over the range of pH5-7 at 50℃ for 1 hour. But half of its activity was lost after 2 hour. The km value was calculated to be 1.2mg of CMC/㎖.

대전광역 시민의 골다공증 검진에 관한 고찰

유은주,이관우,박현모,이수배,김광현,강희구,박성오 한국건강관리협회 1999 건협 Report Vol.2 No.-

로타바이러스 원내 감염과 지역사회 획득 감염의 비교 연구

유재성,진현주,강호석,오지은 고신대학교 의과대학 2010 고신대학교 의과대학 학술지 Vol.25 No.2

Purpose: This study was performed to investigate the epidemiologic and clinical characteristics of rotaviral infection, the most common nosocomial gastroenteritis. Method: We retrospectively reviewed the records of 983 patients who were diagnosed as rotavirus gastroenteritis from January 2006 to March 2010. We divided them into two groups: group A for community-acquired rotavirus infection (N=810), group B for nosocomial infection (N=173). We investigated the characteristics of both groups. Results: The percentage of nosocomial infection among rotavirus gastroenteritis was 18% in our study. Rotaviral infection in both groups was more common in winter. The average age (±SD) of group A and B was 25.8±23.3 and 17.8±15.7 months old, respectively. The average hospital days (±SD) were 4.3±2.0 days for group A and 9.0±3.5 days for group B. Nosocomial infection symptoms of group B started at 4.6±1.7 hospital days on average. Fever, vomiting, abdominal pain, convulsion, elevation of serum CRP (C-reactive protein) or AST (Aspartate aminotransferase) were observed more common in group A than B. Conclusion: The nosocomial rotavirus infection rate did not decrease as we expected in spite of our effort of strict hygiene control. Nosocomial infection showed more favorable prognosis than community-acquired infection but demanded longer days of hospitalization and more cost.

면접조사를 통한 초등학생의 영양교육 실태 및 실천도 조사

오유진,이영미,김정현,안홍석,김정원,박혜련,서정숙,김경원,권오란,박혜경,이은주,성현이 대한지역사회영양학회 2008 대한지역사회영양학회지 Vol.13 No.4

This study investigated the experience and practice of elementary school students on nutrition education. The data were collected from 217 male and female students attending 5-6th grade elementary schools in Seoul and Kyunggi-Do from March to June 2007, interviewing face to face by a nutrition teacher and 3 interns of a nutrition teacher. The results were as follows: 86.5% of the subjects learned about ‘Table etiquette’, ‘Reasons for eating fruits and vegetables’ (78.7%), ‘Food waste and environment’ (72.3%), ‘Healthy snacks’ (55.7%), ‘Food sanitation’ (52.3%), ‘Food culture of foreign countries’ (48.1%). Nutrition education experience was significantly different by gender. A total of 43.5% boys responded that they never learned about ‘basic food preperation’ (p < 0.01). They had learned ‘Nutrients for body’ and ‘Food waste and environment’ in school, ‘Healthy weight loss’, ‘Food culture of foreign countries’, ‘Food circulation’ on television, Most content (‘Table etiquette’, ‘Simple cooking’, ‘Food sanitati n’, ‘Eating behaviors for health’, ‘Reasons for eating fruits and vegetables’, ‘Healthy snacks’) was learned from parents. The practice after nutrition education was higher in ‘Table etiquette’ (2.14), ‘Eating fruits and vegetables’ (2.07) than others compared with education experience. The most reason of non-practice on nutrition information was ‘Troublesome’. In ‘Nutrients for body’, a boy answered ‘Difficult for practice’ 20.0%, a girl answered ‘Difficult to understand’ 32.6%, showing a significant difference between the gender groups (p < 0.001). They remembered the ‘Nutrients for body’ (49.6%), ‘Food sanitation’ (44.5%) because of ‘important content’,‘Basic food preparation’ (40.6%), ‘Food culture of foreign countries’ (36.3%) because of ‘interesting content’, ‘Healthy weight loss’ (52.0%), ‘Eating behavior for health’ (44.5%) and ‘Healthy snacks’ (33.7%) because of ‘need for my health’.

무선 ATM에서의 동적 슬롯 할당 알고리즘

이지현,유소영,서주하 江原大學校 産業技術硏究所 2001 産業技術硏究 Vol.21 No.A

In recent years, the predominant part in telecommunications is mobile communications. The next generation network is extending today's voice-only mobile networks to multi-service networks. ATM Network is possible to carry such multi-media traffic and it will be expect to use wireless ATM for the future mobile access network. One of manly important aspects for the performance of wireless ATM is the Medium Access Control (MAC) protocol. The MAC protocol must be able to satisfyingly handle the different ATM services (CBR, VBR, ABR and UBR) with their radically different performance requirements. Additionally, the MAC protocol must be able to cope with the complex radio environment where fading, multi-path propagation interference and burst-errors further complicate the situation. In this paper, a dynamic slot allocation algorithm in wireless ATM is proposed for an efficient channel sharing/media access at the MAC layer. We use equivalent capacity in the allocation of slots for VBR traffic which is variable along the time. It is simple and effective slot allocation method for VBR service. In particular, we consider the slot allocation of a session consisted of several connections for requirement of multimedia traffic. Simulation shows that the cell loss ratio is reduced by re-allocation of extra slots in Mobile Terminal (MT).