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유주현,박연규 아시아교정포럼 2022 교정담론 Vol.16 No.1
본 연구는 자살시도를 한 환자들 대상으로 죽음 관에 대하여 자살위기 개입 상담경험 토대로 현상학 방법으로 살펴보고자 한다. 이를 위하여 최근 1년 사이에 실제 자살시도를 한 후, 최소 병원 중환자 응급실에서 치료를 받은 경험이 있는 내담자 대상으로 표본을 추출 하였다. 연구 참여에 동의한 6명의 참여자 들과 3회의 면담을 실시한 후 면담자료를 토대로 분석 및 해석 하였다. 면담자료 이외에도 연구주제와 관련된 문헌 자료를 살펴 보고 내담자들의 상담과 관련된 다양한 자료들을 수집하여 분석 및 해석을 하는데 활용하였다. 연구 결과로는 자살 시도자의 상담경험은 “죽음 결정”이라는 핵심 주제 아래 “존재의 부정”과 관련된 주제와 “현존의 실체”에 대해서 나누고 “부활의미‘ 와 같은 네 가지의 본질적 주제를 살펴보았다. 그리고 자살 위험성 의 면담 내용인 자살생각, 자살계획, 자살의도에 대해서 살펴 보고 ‘육신이 소멸되면 영혼도 사라진다.’,‘나를 부정하고 죽음 으로 내 던지다.’,‘나는 죽었지만 여전히 살아 있다.’,‘죽음을 통해서 나를 찾다.’에 관한 하위 주제로 나누었다. 이와 같은 결과를 토대로 죽음을 결정한 내담자에 대한 이해와 자살 예방 상담에 시사 하는 것을 논의하였고 후속 연구를 제안하였다. In order to understand what counseling for suicide crisis intervention meant to the clients who decided to death, this study aims to examine the counseling experience of the clients who decided to death in a hermeneutic phenomenological method. Methods: To this end, samples were collected from clients who were treated at least in the emergency room of the hospital's intensive care unit after attempting an actual suicide last year, and analysis and interpretation were conducted based on the interview data after three interviews. Results: It was found that the client who made a suicide attempt in the past and decided to die has a view on death that has the concept of 'nothing', in which my body and soul disappear when I die, rather than on me. Conclusion: Understanding of the client who decided to die and suggesting suicide prevention counseling were discussed, and follow-up studies were proposed.
Cellulase생산을 위한 Verticillium sp.의 배양조건의 검토
홍대희,진효상,오두환,유주현 연세대학교 산업기술연구소 1986 논문집 Vol.18 No.1
The cultivation conditions on wheat bran of verticillium sp. were optimized to produce cellulase as much as possible. The highest cellulase activity was shown, as verticillium sp. was cultivated at 27℃ for 5 days on wheat bran media with 1.2㎖/g wheat bran of water content and supplementation of NaNo and FeSo, in concentration of 0.1% and 0.2-0.3%, respectively.
微生物凝乳酵素 Mucor-renin이 k-casein에 對한 作用
柳洲鉉,有馬啓 연세대학교 대학원 1976 延世論叢 Vol.13 No.2
k-casein was reacted with Mucor rennin(MR) or Calf rennin(CR). Each para-k-casein and macropeptide were separated from the hydrozed k-casein, and the casein were analyzed to elemental composition, composition of amino acids and terminal amino acids. N-terminal of para-k-casien by MR reaction was not confirmed, but Phe and Leu in C-terminal were identified on paper chromatography by examination of para-k-casein with carboxypeptidase. N-terminal of macropeptide was Met. Therefore, MR was hydrolyzed between linkage of Phe in C-terminal of para-k-casein and Met in N-terminal in macropeptide. Results of substrate specificity on k-casein with CR was corresponded to above results of MR.
미생물을 이용한 생리활성 물질의 생산 Coenzyme Q 발효에 관한 연구
박인수,오두환,변유량,유주현 연세대학교 산업기술연구실 1979 논문집 Vol.11 No.1
Candida 속 13주 Saccharomyces속 13주, Rhodotorula속 2주,Debaromyces속 1주, Hansenula속 1주 등의 균주를 이용하여 Coenzyme Q를 생산하는 Rhodotorula rubra 1526을 선정하였다. 그리고 이 미생물을 이용하여 Coenzyme Q 생산량을 높이기 위한 배지조성과 배양조건을 검토하였다. 배지속의 탄소원으로는 glucose 6.0W/V%, 질소원은 urea 0.2w/v(N기준)이었으며 Mg는 MgCl₂·6H₂O 0.04W/v%, 천연물로는 yeast extract 0.4W/v%, pH5.0이었다. 또한 Coenzyme Q의 전구물질로 알려져 있는 p-hydroxybenzoic acid는 배지 1ℓ당 10mg을 가했을 때 약간의 증대가 있을뿐 그다지 효과가 크지 않았고 그밖의 생육인자도 요구하지 않았다. 이와같이 배양한 결과 처음 screening 당시보다 35.11mg/ ℓ-broth의 Coenzyme Q 생산의 증대를 보여 brothℓ당 41.11mg의 Coenzyme Q를 생산하였다. The microorganisms that produces Coenzyme Q, have been selected from 13 strains of genera Candida, 13 strains of genera Saccharomyces, 2 strains of genera Rhodotorula 1 strain of genera Debaramyces, 1 strains of Hansenula. Five strains of yeast, Candida tropicalis Candida parakrusei 1529, Rhodotorula rubra 1526, Saccharomyces cerevisiae 1531 and Schizosaccharomyces pombe 1524 were produced relatively large amounts of Coenzyme Q. Especially Rhodotorula rubra 1526 was showed the highest Coenzyme Q productivity. By using Rhodotorula rubra 1526, the optimum conditions for the production of Coenzyme Q on the rotary shaker were pH, 5.0; glucose 6.0w/v%; urea 0.2w/v%; MgCl₂·6H₂O 0.04w/v% ·yeast extract 0.4w/v%; at 30℃, for 36hrs, respectively. On the study of time course revealed that there were two peaks in the Coenzyme Q production: one at the late exponential growth phase and other at the stationary phase.
폐기물 소각시 생성되는 유해 중금속물질과 연소실내 비산재와의 응축, 응집 현상에 대한 연구
유주현,황정호,Yu, Ju-Hyeon,Hwang, Jeong-Ho 대한기계학회 1997 大韓機械學會論文集B Vol.21 No.2
A numerical analysis on condensation and coagulation of the metallic species with fly ash particles pre-existing in an incinerator was performed. Waste was simplified as a mixture of methane, chlorine, and small amounts of Pb and Sn. Vapor-phase amounts of Pb- and Sn -compounds were first calculated assuming a thermodynamic equilibrium state. Then theories on vapor-to-particle conversion, vapor condensation onto the fly ash particles, and particle-particle interaction were examined and incorporated into equations of aerosol dynamics and vapor continuity. It was assumed that the particles followed a log-normal size distribution and thus a moment model was developed in order to predict the particle concentration and the particle size distribution simultaneously. Distributions of metallic vapor concentration (or vapor pressure) were also obtained. Temperature drop rate of combustion gas, fly ash concentration and its size were selected as parameters influencing the discharged amount of metallic species. In general, the coagulation between the newly formed metal particles and the fly ash particles was much greater than that between the metal particles themselves or between the fly ash particles themselves. It was also found that the amount of metallic species discharged into the atmosphere was increased due to coagulation. While most of PbO vapors produced from the combustion were eliminated due to combined effect of condensation and coagulation, the highly volatile species, PbCl$_{2}$ and SnCl$_{4}$ vapors tended to discharge into the atmosphere without experiencing either the condensation or the coagulation. For Sn vapors the tendency was between that of PbO vapors and that of PbCl$_{2}$ or SnCl$_{4}$. To restrain the discharged amount of hazardous metallic species, the coagulation should be restrained, the number concentration and the size of pre-existing fly ash particles should be increased, and the temperature drop rate of combustion gas should be kept low.