Mongolian Gerbil과 CJU/A Mouse의 와우에서 βNADPH Diaphorase의 발현 양상

추무진,김진섭,송창근,신시옥,진홍률 대한이비인후과학회 1999 대한이비인후과학회지 두경부외과학 Vol.42 No.11

先加力 후 補修-補强한 鐵筋콘크리트 壓縮部材의 構造特性에 관한 實驗的 硏究

신용석,최진석,김판선,조철희,손순채,김정섭 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2(구조계)

This study examined the Stress and structural characteristics of reinforced concrete compressive member repair and strengthening by CFS and GFS after pre-loading. The following results were obtained. The results of compressive testing by the kind of fiber suggested that: In CFS, Specimen increased by about 26.9% and strengthening after pre-loading, by about 111.8%, compared with after strengthening, In GFS, Specimen increased by about 69.0% and repaired and models after pre-loading, by about 76.7%, compared with after strengthening. In the compressive testing, strengthen with CFS-Specimen showed a brittle fracture and strengthen with GFS-Specimen represented ductile fracture. More increasing in stress of Specimen, and repair and Strengthening specimen after pre-loading than loading specimen after strengthening suggested the strengthen effect of fiber.

흰쥐에 對한 Phosphamidon 및 Endosulfan의 亞急性毒性에 關한 硏究

申鎭燮,崔承允,李昌業,崔仁厚 서울大學校 農科大學 1984 서울대농학연구지 Vol.9 No.2

Subacute toxicity tests of phosphamidon and endosulfan were carried out to examine the adverse effects resulting from repeated daily oral administration of low dose levels (1, 2 and 5 mg/kg/day) to rats for 28 days. The results were summarized as follows: 1. Rats showed clinical signs of salivation hyperactivity, ataxia at 2mg/kg/day dosage of phosphamidon and, in addition, lachrymation, tremor, exophthalmus and diarrhea at the highest level. On the other hand, endosulfan caused only hyperactivity in rats treated with the highest dosage. There was no mortality at any dosage of either pesticide. 2. Rats gained significantly lower body weight, when treated at the 5mg/kg/day dose levels of phosphamidon and endosulfan, in comparison to normal, nontreated rats. 3. Plasma cholinesterase activity was significantly depressed at all levels of phosphamidon during the administration period, but returned to normal within 2 weeks after the last administration. Endosulfan did not change plasma cholinesterase activity at any levels. 4. Leukopenia was detected only in rats treated with phosphamidon at 5mg/kg/day level. No other adverse changes in hematology were observed in rats treated either with phosphamidon at lower levels or with endosulfan at any levels. 5. Cloudy swelling of liver was the only histological change in rats treated with phosphamidon or endosulfan at the highest level. No other histopathological change in rats treated with phosphamidon or endosulfan at the highest level. No other histopathological symptoms were found in spleen, heart, kidney, and bone marrow of rats treated. 6. It may be concluded from the results above that the "no effect level" is 1 mg/kg/day and 2 mg/kg/day for phosphamidon and endosulfan, respectively.

대관령의 일조시간을 이용한 월별 일사량의 추정

박진기, 오시영, 백신철, 김봉섭, 신형섭, 박종화 忠北大學校 農業科學硏究所 2013 農業科學硏究 Vol.29 No.1

The monthly mean daily global solar radiation and sunshine duration, have been employed in this study to develop correlation model for Daegwallyeong during 2011. The corresponding monthly value of extraterrestrial solar radiation and possible duration of sunshine are calculated for Daegwallyeong meteorological data. Forecasting performance parameters such as coefficient of determination (R2), mean bias error (MBE) and root mean square error (RMSE) are presented as analyzed for the model. The model with multi-linear regression has the highest R2 value of 0.95 and average MBE and RMSE values of −0.11 MJ/m2day and 1.63 MJ/m2day, respectively. The model exhibited negative MBE which shows that the model resulted in underestimation of the global solar radiation relative to the meteorological measured value. The equation could be employed in the estimation of solar radiation at Daegwallyeong, other locations with similar meteorological characteristics.

혈액암환자에서 조혈모세포 이식 후 따르는 헤르페스 바이러스 감영 양상

이호섭,탁희상,신성훈,김양수,남성진,김혜수,박진희,정수현,김성빈,김예나 고신대학교 의과대학 2010 고신대학교 의과대학 학술지 Vol.25 No.1

배경 : 헤르페스바이러스과에는 단순포진 바이러스(HERPES SIMPLEX VIRUS, HSV), 수두대상포진 바이러스 (VARICELLA-ZOSTER VIRUS, VZV), 엡스타인-바 바이러스 (EPSTEIN-BARR VIRUS, EBV), 그리고 거대세포 바이러스 (CYTOMEGALOVIRUS, CMV) 등이 있다. 헤르페스바이러스 감염은 혈액암환자에서 조혈모세포 이식 환자에서 전파 (dissemination), 이차감염, 세균 중복 감염의 심각한 합병증을 일으킬 수 있다. 하지만 항암약물치료를 받는 림파종 환자에서 항 바이러스 약제의 사용 용량과 기간에 대한 concensus는 없다. 헤르페스바이러스 감염에 대한 발생율과 위험인자를 후향성으로 분석하였다. 방법 : 고신대학교 복음 병원에서 1995년 6월부터 2009 년 2월 사이에 새롭게 진단되어 조혈모세포 이식을 받은 전체 58명의 환자들이 후향적으로 현 연구에 등록했다. 헤르페스 바이러스 감염은 임상적 진단, 혈청학적 검사 또는 병리학적 진단에 기초하여 확진한다. 환자들의 특성은 다음과 같다: 평균 연령은 44세 (범위 19-62세)였고 남녀비는 30:28이었다. 등록된 질환은 백혈병 (n=9, 15.5%), 림프종 (n=30, 51.7%), 다발성 골수종 (n=12, 20.7%), 재생불량성 빈혈 (n=6, 10.3%) 그리고 골수이형성증후군 (n=1, 1.7%)이 포함되었다. 결과는 chi-squre test나 independent samples T test를 사용하여 분석되었다. Multivariate analysises에 대해 logistic regression test를 사용하였다. 결과 : 15명의 환자들 (25.9%)에서 조혈모세포 이식후 헤르페스 바이러스 감염이 발생하였다. 조혈모세포 이식 5년간 누적 발생율은 53.9%였다. Univariate analyses에서 헤르페스 바이러스 감염에 대한 유의한 위험 인자는 없었다. 하지만 조혈모세포 이식후 생존 기간(40.18 ± 30.14 months vs 20.06 ± 26.67 months, p=0.018)은 헤르페스 바이러스 감염의 낮은 발병율과 유의한 관련성이 있었다. 이식 편대 숙주질환 (GVHD)의 존재 (37.5% in developed GVHD vs 0% in no GVHD, p=0.200), 동종조혈 모세포 이식에서 면역억제요법의 기간 (15.98 ± 14.02 months vs 6.78 ± 3.67 months, p = 0.374)은 헤르페스 바이러스 감염에 대한 위험인자가 아니었다. 결론 : 조혈모세포 이식 후 생존 기간은 어사이클로비어 예방을 받은 혈액암 환자에서 헤르페스 바이러스 감염의 낮은 발병율과 연관성이 있는 것으로 보인다. 저용량의 어사이클로비어 예방은 헤르페스 바이러스 감염의 고위험군인 조혈모세포 이식을 받은 혈액암 환자에 필요하리라 생각된다. Background : Herpesviridae family includes herpes simplex virus, varicella zoster virus, Epstein-Barr virus, and cytomegalovirus, etc. Herpesviridae viral infection (HVI) can lead to serious complications including dissemination, secondary infection, bacterial superinfection in patients with hematologic malignancy following hematopoietic stem cell transplantation (HSCT). But there was no consensus on the dose and duration of antiviral agents prophylaxis in patients undergoing chemotherapy. We retrospectively analyzed the incidence and the risk factors for HVI. Method : A total of 58 patients who newly diagnosed and received HSCT with prophylaxis of acyclovir at the Kosin University Gospel Hospital, Busan, Korea between June 1995 and February 2009 were enrolled retrospectively in the current study. HVI was confirmed based on clinical diagnosis, serologic test or pathologic diagnosis. The characteristics of the patients were as follows: the median age was 44 years (range 19-62 years) with a female-to-male ratio of 30:28. The enrolled diseases included leukemia (n=9, 15.5%), lymphoma (n=30, 51.7%), multiple myeloma (n=12, 20.7%), aplastic anemia (n=6, 10.3%) and myelodysplastic syndrome (n=1, 1.7%). The results were analyzed using a chi-square test and independent samples T test. For the multivariate analysis, we used logistic regression test. Results : Fifteen patients (25.9%) developed HVI after HSCT. The cumulative incidence of HVI was 53.8% at 5 years after HSCT. In univariate analysis, there was no significant risk factor for HVI. The presence of graft-versus-host disease (GVHD) (37.5% in developed GVHD vs. 0% in no GVHD, p=0.200), duration of immunosuppressive therapy (IST) in allo-SCT (15.98 ± 14.02 months vs. 6.78 ± 3.67 months, p = 0.374) were not risk factors for HVI. Conclusion : The incidence of HVI was similar to that in historical other studies. There was no risk factor associated with development of HVI. Most of the HVI occurred within the first 24 months after transplantation. So long term use of antiviral prophylaxis may be needed to prevention of HVI after HSCT.

자주달개비 미세핵 분석법을 이용한 비스페놀 에이 및 감마선의 생물학적 영향 비교 연구

신해식,송희섭,현성희,이진홍,김진규 한국환경생물학회 2002 환경생물 : 환경생물학회지 Vol.20 No.2

합성화학물질의 몇몇은 고등동물의 내분비계를 교란하는 물질로 작용하는 것이 알려져 있다. 다이옥신,디디티,피시비 및 비스페놀 에이 등이 내분비계 장애물질로 분류되어 선진 각국에서는 엄격한 통제하에 관리되고 있다. 본 연구는 자주달개비 미세핵 분석법을 이용하여 염색체이상 유발에 있어서의 BPA와 방사선의 상대적 효과를 비교하기 위하여 시행되었다 흡수처리를 위하여 자주달개비 4430클론의 절취화서를 각각의 농도로 조제된 BPA용액에 여섯시간 동안 침지하고 폭기를 실시하였다. 한편 방사선의 영향을 평가하기 위하여 동일 식물체의 화서군을 양액에 침지한 상태로 O∼0.5 Gy까지 선량별로 조사하였다. 실험선량의 범위에서 미세핵 생성률은 분명한 선량-반응 관계를 나타내었으며 BPA 실험농도 범위에 대해서도 농도-반응 관계를 나타내었다. 이들 두가지 반응 관계식을 이용하여 동일한 미세핵 빈도를 유발하는 방사선량과 비스페놀 에이 농도를 산출할 수가 있는데, BPAll.8μM은 방사선 피폭후 임상증상이 나타나기 시작하는 선량인 0.25Gy에 상응하는 염색체 이상을 유발하는 것으로 확인되었다. 본 연구의 결과를 통하여 자주달개비 화분모세포는 비스페놀 에이, 욱틸페놀 또는 노닐페놀 등의 내분비계 장애물질의 생물학적 독성을 평가하는데 유용하게 활용할 수 있는 생물말단 점이라는 것이 입증되었다. Some of synthetic chemicals can act as an endocrine disrupting substance in higher animals. Dioxins, DDT, PCBs and bisphenol A (BPA) are classified into endocrine disruptors and are under a strict control in many countries. This research was designed to compare the clastogenic effects of BPA to those of ionizng radiation to establish the relative effectiveness of BPA by means of Tradescantia micronucleus assay. For the uptake of the BPA through the stems, groups of fresh cuttings of Tradescantia BNL 4430 were placed in BPA solutions of 0 to 4 ??M for 6 hours under continuous aeration. The other groups of the cuttings were irradiated with 0 to 0.5 Gy of gamma-rays. The frequencies of micronucleus showed a positive dose-response relationship in the range of 0 to 0.5 Gy, and a clear concentration-response relationship in the experimental range of BPA concentrations. By comparing the two experimental results, it is possible to estimate the BPA concentration and its equivalent radiation dose for a fixed value of MCN frequency. BPA of 11.8 ??M can give rise to 53.3 MCN/100 tetrads, which is the same frequency induced by 25 cGy of gamma-rays. It is of biological importance that clinical symptoms start to develop after a whole body exposure to radiation higher than 25 cGy. The results indicate that the pollen mother cells are an excellent biological end-point for toxicity test of suspected endocrine disrupting chemicals such as bisphenol A, cotylphenol and nonylphenol.

건강인의 비인강에서 황색포도구균의 분리와 Methicillin 내성 Staphylococcus Aureus의 항균제 감수성

김신무,정수진,정재옥,신용섭 원광보건전문대 1995 원광보건전문대학논문집 Vol.18 No.-

鹽化物 含有梁에 따른 鐵分 腐蝕에 관한 實驗的 硏究

최진석,조철희,신용석,고송균,손순채,김정섭 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1(구조계)

As a result of separating river sand and sea sand into the corrosion increased according to the of thinnss or the coating and the Chloride Content and Corrosion of Steel Bar through the experiment, the following conclusions are obtained. 1. There was no corrosion in the case of treating the steel bar with anticorrosive among test specimens cured in the air for 60 days, corrosion was found in the test specimens not treated with anticorrosive and for 60 days of age. 2. The area of corrosion increased according to the thinnss of the coating and the chloride content wher exposed for are more than 60 days. Therefore, it was determined in general that the corrosion progressed more rapidly in water cured specimens than in air cured ones and this was due to the influence of C1_(￣) in the sea sand.

Lidocaine, Thrombin, Epinephrine 의 항균효과

김진우,이동건,전혜선,김승준,김석찬,안중현,김치홍,권순석,김영균,김관형,문화식,신완식,송정섭,박성학 대한감염학회 2005 감염과 화학요법 Vol.37 No.6

목적 : 기관지내시경 검사에서 흔히 사용되는 국소마취제인 lidocaine과 내시경시 지혈목적으로 사용되는 thrombin과 epinephrine이 각종 균주에 미치는 항균효과에 대해 알아보고자 하였다. 재료 및 방법 : 균주는 가톨릭대학교 성모병원에서 2004년 3월부터 2004년 9월까지 임상검체 에서 동정된 S. aureus, S. pneumoniae, K. pneumoniae, P. aeruginosa 각각 42, 42, 42, 43주를 대상으로 하였다. Lidocaine, thrombin, epinephrine 감수성 검사는 National Committee for Clinical Laboratory Standards (NCCLS, 2002)의 기준에 따랐다. 결과 : Lidocaine은 S. aureus, S. pneumoniae, P. aeruginosa에서 MIC_(50), MIC_(90) 모두 20,000 ㎍/mL 이었다. K. pneumoniae는 각각 10,000 ㎍/mL이었다. Thrombin은 S. aureus와 P. aeruginosa에서 MIC50 500 lU/mL 과 MIC_(90) 500 IU/mL 이상이었고, K. pneumoniae에서는 MIC_(50)과 MIC_(90)이 모두 500 lU/mL이상이었으나 S. pneumoniae에서는 MIC_(50)과 MIC_(90)은 125 IU/mL이었다. Epinephrine은 K. pneumoniae, S. pneumoniae에 대한 MIC_(50), MIC_(90)가 모두 >500 ㎍/mL이었고, S. aureus와 P. aeruginosa에 대한 MIC_(50), MIC_(90)가 모두 500 ㎍/mL이었다. 결론 : 기관지 내시경 검사에서 흔히 쓰이는 lidocaine, thrombin, epinephrine 등의 약제들이 호흡기 질환의 흔한 병원균인 S. aureus, S. pneumoniae, K. pneumoniae, P. aeruginosa 균주들에 대해 항균 효과가 있을 수 있어 세균배양검사에 영향을 미칠 수 있겠다. Background : We performed this stody to find out about antimicrobial effect of lidocaine which is commonly used local anesthetic, and thrombin and epinephrine used for hemostasis during bronchoscopic procedures. Materials and Methods : The microorganisms that were cultured from specimens obtained during bronchoscopy were Staphylococcus aureus (n=42), Streptococcus pneumoniae (n=42), Klebsiella pneumoniae (n=42), and Pseudomonas aeruginosa (n=43) collected from St. Mary's Hospital, from March to Sep 2004 were used for susceptibity testing. Susceptibility to lidocaine, thrombin, and epinephrine were tested according to the National Committee for Clinical Laboratory Standards. Result : MIC_(50) and MIC_(90) of lidocaine for S. aureus, S. pneumoniae, P. aeruginosa were all 20,000 ㎍/mL and that for K. pneumoniae were 10,000 ㎍/mL. MIC_(50) and MIC_(90) of thrombin for both S. aureus and P. aeruginosa was 500 IU/mL and above 500 IU/mL, respectively; that for K. pneumoniae were all above 500 IU/mL and for S. pneumoniae they were 125 IU/mL, MIC_(50) and MIC_(90) of epinephrine for K. pneumoniae and S. pneumoniae were above 500 ㎍/mL; that for S. aureus and P. aeruginosa were 500 ㎍/mL. Conclusion : We observed possible antimicrobial effect of lidocaine, thrombin, and epinephrine in vitro against pathogens such as S. aureus, S. pneumoniae, K. pneumoniae, P. aeruginosa, which are common respiratory microorganisms. The use of these agants could affect the result of bacterial culture.

미니에멀젼 중합을 이용한 상전이 물질의 나노 캡슐화 및 열적특성 연구

김경래,백경현,신진섭,김중현 한국공업화학회 2004 응용화학 Vol.8 No.2

The nanocapsule containing phase change materials (PCM) such as octadecane were prepare by miniemulsion polymerization. The size, shape, and encapsulation efficiency of PCM nanocapsule was analyzed by scanning electron microscope (SEM), transmission electron microscope (TEM), hydrodynamic fractionation (CHDF), differential scanning calorimeter (DSC) and thermogravimetric analysis (TGA). PCM nanocapsule size range from 50 to 100 ㎚, and its morphology was shapes. The maximum encapsulation efficiency of PCM materials were around 90%.