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금은화 Ethyl Acetate분획이 인체 구강유상피암종세포에 미치는 항암효과
백경현,한두석 원광대학교 생체재료·매식연구소 1997 원광생체재료·매식 Vol.6 No.2
This study was carried out to develop antitumor agents based on effects of the ethyl acetate soluble fraction of methanolic extract of Lonicerae flos on human oral epitheloid carcinoma cells. Human oral epitheloid carcinoma cells were cultured in RPMI-1640 media containing 10% fetal bovine serum, antibiotic, and fungizone. After incubation for 24 hr, the cells were treated with each fraction for 48 hr under the same condition. MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide), NR (Neutral red) and SRB (Sulforhodamine B protein) assay were performed. The light microscopic findings were observed by inverted microscope. These results were obtained as follows; 1. In MTT assay, fraction B was shown significant antitumor activity (p<0.01), fraction E was shown significant antitumor activities (p<0.05), but the other fractions were not shown. 2. In NR assay, fraction B was shown significant antiumor activity (p<0.001). 3. In SRB assay, fraction B was shown significant antitumor activities (p<0.01), fractions A and D were shown significant antitumor activities (p<0.05), but the other fractions were not shown. 4. In light microscopy, fraction B of the ethyl acetate soluble fraction showed the highest antitumor activity on Lonicerae flos. These findings suggested that fraction B possessed the most antitumorous agent.
백경현 한국전통문화대학교 한국전통문화연구소 2014 한국전통문화연구 Vol.13 No.-
마미체는 전통공예로서 그 전승이 불투명한 귀중한 무형문화재이다. 그 역사와 의미 그리고 세계 속의 위치로 볼 때 기존에 이미 사라져간 여러 가지 공예기술과는 구별되 게 그 존재가 특별하다. 기록상으로도 다양한 근거를 찾을 수 있으며, 그 실용성 또한 우리 삶의 가운데 자리 잡고 있었다. 특히 세계 거의 모든 나라에 마미체라는 공예가 존재해 왔으며 그 일부는 스스로 전통공예라 하여 전승되는 원형을 볼 수 있으나 그 기능면이나 예술성, 조형성, 심미성에서 우리의 전통공예 마미체와 비교를 거부한다. 실로 다양한 종류가 존재했으며 사용 또한 다양하게 하였다. 세시풍속에 나타날 정도 로 생활 깊숙이 그 존재감을 보인다. 더불어 전승을 고민하며 대안을 제시했다. Horsetail hair sieve is one of famous traditional handicrafts technologies in world wide. As an important intangible cultual technology, nobody commit to transmit to next generations in Korea. It has very long history and many records in book. Even having very useful practical uses in kitchen, it turn over to plastics and/or stainless steel sieve, etc. Horsetail hair sieve of Korea is very special like frame, artistry, and good design, And it has 100% environment friendly materials, horsetail hair as mash, pine tree as wheel frame, pine tree roots as cord, bamboo as nail, and Korea lacquer as painting like that. It means there is no potential chemical reaction and some allergies. Also, we have some legends in customs like Rome and Japan, too. With this research, I would like to study how to transmit to next generations this kind of important intangible properties technologies and preservation as global intangible properties.